Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiC and thiE overlap by 1 nucleotides thiE and thiF overlap by 8 nucleotides thiF and thiS overlap by 17 nucleotides thiS and thiG are separated by 1 nucleotides thiG and thiH overlap by 4 nucleotides
MCAODC_03875: thiC - phosphomethylpyrimidine synthase ThiC, at 781,228 to 783,123
thiC
MCAODC_03880: thiE - thiamine phosphate synthase, at 783,123 to 783,758
thiE
MCAODC_03885: thiF - thiazole biosynthesis adenylyltransferase ThiF, at 783,751 to 784,506
thiF
MCAODC_03890: thiS - sulfur carrier protein ThiS, at 784,490 to 784,690
thiS
MCAODC_03895: thiG - thiazole synthase, at 784,692 to 785,462
thiG
MCAODC_03900: thiH - 2-iminoacetate synthase ThiH, at 785,459 to 786,592
thiH
Position (kb) 783
784
785 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 782.758 kb on - strand, within thiC at 782.758 kb on - strand, within thiC at 782.769 kb on + strand, within thiC at 782.769 kb on + strand, within thiC at 782.769 kb on + strand, within thiC at 782.770 kb on - strand, within thiC at 782.770 kb on - strand, within thiC at 782.776 kb on + strand, within thiC at 782.776 kb on + strand, within thiC at 782.776 kb on + strand, within thiC at 782.777 kb on - strand, within thiC at 782.777 kb on - strand, within thiC at 782.777 kb on - strand, within thiC at 782.777 kb on - strand, within thiC at 782.777 kb on - strand, within thiC at 782.778 kb on + strand, within thiC at 782.778 kb on + strand, within thiC at 782.778 kb on + strand, within thiC at 782.778 kb on + strand, within thiC at 782.778 kb on + strand, within thiC at 782.778 kb on + strand, within thiC at 782.778 kb on + strand, within thiC at 782.779 kb on - strand, within thiC at 782.779 kb on - strand, within thiC at 782.886 kb on + strand, within thiC at 782.886 kb on + strand, within thiC at 782.887 kb on - strand, within thiC at 782.952 kb on + strand at 782.953 kb on - strand at 783.042 kb on + strand at 783.042 kb on + strand at 783.056 kb on + strand at 783.063 kb on + strand at 783.063 kb on + strand at 783.064 kb on - strand at 783.100 kb on + strand at 783.126 kb on + strand at 783.126 kb on + strand at 783.126 kb on + strand at 783.127 kb on - strand at 783.127 kb on - strand at 783.148 kb on + strand at 783.148 kb on + strand at 783.187 kb on + strand, within thiE at 783.187 kb on + strand, within thiE at 783.187 kb on + strand, within thiE at 783.187 kb on + strand, within thiE at 783.188 kb on - strand, within thiE at 783.188 kb on - strand, within thiE at 783.188 kb on - strand, within thiE at 783.188 kb on - strand, within thiE at 783.188 kb on - strand, within thiE at 783.220 kb on + strand, within thiE at 783.220 kb on + strand, within thiE at 783.220 kb on + strand, within thiE at 783.220 kb on + strand, within thiE at 783.220 kb on + strand, within thiE at 783.221 kb on - strand, within thiE at 783.221 kb on - strand, within thiE at 783.221 kb on - strand, within thiE at 783.221 kb on - strand, within thiE at 783.224 kb on + strand, within thiE at 783.225 kb on - strand, within thiE at 783.236 kb on - strand, within thiE at 783.307 kb on - strand, within thiE at 783.495 kb on + strand, within thiE at 783.495 kb on + strand, within thiE at 783.495 kb on + strand, within thiE at 783.495 kb on + strand, within thiE at 783.495 kb on + strand, within thiE at 783.495 kb on + strand, within thiE at 783.495 kb on + strand, within thiE at 783.495 kb on + strand, within thiE at 783.496 kb on - strand, within thiE at 783.496 kb on - strand, within thiE at 783.496 kb on - strand, within thiE at 783.496 kb on - strand, within thiE at 783.496 kb on - strand, within thiE at 783.496 kb on - strand, within thiE at 783.496 kb on - strand, within thiE at 783.497 kb on + strand, within thiE at 783.598 kb on - strand, within thiE at 783.689 kb on + strand, within thiE at 783.690 kb on - strand, within thiE at 783.776 kb on - strand at 783.777 kb on + strand at 783.870 kb on + strand, within thiF at 783.870 kb on + strand, within thiF at 783.886 kb on + strand, within thiF at 783.886 kb on + strand, within thiF at 783.944 kb on + strand, within thiF at 783.945 kb on - strand, within thiF at 784.058 kb on + strand, within thiF at 784.058 kb on + strand, within thiF at 784.058 kb on + strand, within thiF at 784.058 kb on + strand, within thiF at 784.058 kb on + strand, within thiF at 784.058 kb on + strand, within thiF at 784.059 kb on - strand, within thiF at 784.059 kb on - strand, within thiF at 784.059 kb on - strand, within thiF at 784.059 kb on - strand, within thiF at 784.059 kb on - strand, within thiF at 784.059 kb on - strand, within thiF at 784.059 kb on - strand, within thiF at 784.059 kb on - strand, within thiF at 784.059 kb on - strand, within thiF at 784.085 kb on + strand, within thiF at 784.085 kb on + strand, within thiF at 784.086 kb on - strand, within thiF at 784.135 kb on - strand, within thiF at 784.135 kb on - strand, within thiF at 784.135 kb on - strand, within thiF at 784.155 kb on + strand, within thiF at 784.188 kb on + strand, within thiF at 784.229 kb on + strand, within thiF at 784.230 kb on - strand, within thiF at 784.230 kb on - strand, within thiF at 784.368 kb on + strand, within thiF at 784.373 kb on + strand, within thiF at 784.373 kb on + strand, within thiF at 784.384 kb on - strand, within thiF at 784.385 kb on + strand, within thiF at 784.386 kb on - strand, within thiF at 784.551 kb on + strand, within thiS at 784.552 kb on - strand, within thiS at 784.552 kb on - strand, within thiS at 784.636 kb on + strand, within thiS at 784.636 kb on + strand, within thiS at 784.636 kb on + strand, within thiS at 784.636 kb on + strand, within thiS at 784.636 kb on + strand, within thiS at 784.637 kb on - strand, within thiS at 784.663 kb on - strand, within thiS at 784.696 kb on + strand at 784.696 kb on + strand at 784.697 kb on - strand at 784.697 kb on - strand at 784.700 kb on + strand at 784.700 kb on + strand at 784.700 kb on + strand at 784.771 kb on + strand, within thiG at 784.772 kb on - strand, within thiG at 784.895 kb on + strand, within thiG at 785.102 kb on + strand, within thiG at 785.198 kb on + strand, within thiG at 785.201 kb on + strand, within thiG at 785.296 kb on + strand, within thiG at 785.410 kb on - strand at 785.410 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 782,758 - thiC MCAODC_03875 0.81 -1.4 782,758 - thiC MCAODC_03875 0.81 -2.6 782,769 + thiC MCAODC_03875 0.81 -1.1 782,769 + thiC MCAODC_03875 0.81 -5.5 782,769 + thiC MCAODC_03875 0.81 -4.1 782,770 - thiC MCAODC_03875 0.81 +1.5 782,770 - thiC MCAODC_03875 0.81 -2.2 782,776 + thiC MCAODC_03875 0.82 -3.2 782,776 + thiC MCAODC_03875 0.82 -4.0 782,776 + thiC MCAODC_03875 0.82 -1.6 782,777 - thiC MCAODC_03875 0.82 -2.8 782,777 - thiC MCAODC_03875 0.82 -3.5 782,777 - thiC MCAODC_03875 0.82 +1.8 782,777 - thiC MCAODC_03875 0.82 -4.1 782,777 - thiC MCAODC_03875 0.82 -2.0 782,778 + thiC MCAODC_03875 0.82 -1.4 782,778 + thiC MCAODC_03875 0.82 -2.4 782,778 + thiC MCAODC_03875 0.82 -0.1 782,778 + thiC MCAODC_03875 0.82 -4.5 782,778 + thiC MCAODC_03875 0.82 -1.6 782,778 + thiC MCAODC_03875 0.82 -1.5 782,778 + thiC MCAODC_03875 0.82 +0.4 782,779 - thiC MCAODC_03875 0.82 -1.2 782,779 - thiC MCAODC_03875 0.82 -2.4 782,886 + thiC MCAODC_03875 0.87 -0.7 782,886 + thiC MCAODC_03875 0.87 +0.2 782,887 - thiC MCAODC_03875 0.88 -1.0 782,952 + -0.5 782,953 - -0.2 783,042 + -4.0 783,042 + -0.1 783,056 + -0.3 783,063 + +0.3 783,063 + -4.1 783,064 - +0.2 783,100 + -0.3 783,126 + -1.0 783,126 + -0.3 783,126 + -0.8 783,127 - -0.1 783,127 - -3.9 783,148 + +0.0 783,148 + -2.4 783,187 + thiE MCAODC_03880 0.10 -2.5 783,187 + thiE MCAODC_03880 0.10 +0.0 783,187 + thiE MCAODC_03880 0.10 -0.2 783,187 + thiE MCAODC_03880 0.10 -4.0 783,188 - thiE MCAODC_03880 0.10 -0.7 783,188 - thiE MCAODC_03880 0.10 -0.1 783,188 - thiE MCAODC_03880 0.10 -0.3 783,188 - thiE MCAODC_03880 0.10 -2.3 783,188 - thiE MCAODC_03880 0.10 -3.2 783,220 + thiE MCAODC_03880 0.15 -3.8 783,220 + thiE MCAODC_03880 0.15 -0.3 783,220 + thiE MCAODC_03880 0.15 -2.4 783,220 + thiE MCAODC_03880 0.15 -1.3 783,220 + thiE MCAODC_03880 0.15 -2.2 783,221 - thiE MCAODC_03880 0.15 -2.9 783,221 - thiE MCAODC_03880 0.15 +0.9 783,221 - thiE MCAODC_03880 0.15 -0.4 783,221 - thiE MCAODC_03880 0.15 -2.4 783,224 + thiE MCAODC_03880 0.16 +0.9 783,225 - thiE MCAODC_03880 0.16 -1.4 783,236 - thiE MCAODC_03880 0.18 -0.7 783,307 - thiE MCAODC_03880 0.29 -4.3 783,495 + thiE MCAODC_03880 0.58 -2.6 783,495 + thiE MCAODC_03880 0.58 -3.5 783,495 + thiE MCAODC_03880 0.58 -0.3 783,495 + thiE MCAODC_03880 0.58 -2.2 783,495 + thiE MCAODC_03880 0.58 -1.5 783,495 + thiE MCAODC_03880 0.58 -1.2 783,495 + thiE MCAODC_03880 0.58 -4.1 783,495 + thiE MCAODC_03880 0.58 -0.5 783,496 - thiE MCAODC_03880 0.59 -2.4 783,496 - thiE MCAODC_03880 0.59 -2.5 783,496 - thiE MCAODC_03880 0.59 -0.4 783,496 - thiE MCAODC_03880 0.59 -2.0 783,496 - thiE MCAODC_03880 0.59 -0.2 783,496 - thiE MCAODC_03880 0.59 +0.3 783,496 - thiE MCAODC_03880 0.59 -1.2 783,497 + thiE MCAODC_03880 0.59 +0.1 783,598 - thiE MCAODC_03880 0.75 +0.4 783,689 + thiE MCAODC_03880 0.89 -0.6 783,690 - thiE MCAODC_03880 0.89 +0.3 783,776 - -0.1 783,777 + +2.9 783,870 + thiF MCAODC_03885 0.16 -2.2 783,870 + thiF MCAODC_03885 0.16 -0.0 783,886 + thiF MCAODC_03885 0.18 -1.8 783,886 + thiF MCAODC_03885 0.18 -0.2 783,944 + thiF MCAODC_03885 0.26 -4.5 783,945 - thiF MCAODC_03885 0.26 -0.3 784,058 + thiF MCAODC_03885 0.41 +0.7 784,058 + thiF MCAODC_03885 0.41 -1.4 784,058 + thiF MCAODC_03885 0.41 -3.6 784,058 + thiF MCAODC_03885 0.41 -2.5 784,058 + thiF MCAODC_03885 0.41 -3.1 784,058 + thiF MCAODC_03885 0.41 -0.4 784,059 - thiF MCAODC_03885 0.41 -1.2 784,059 - thiF MCAODC_03885 0.41 -3.7 784,059 - thiF MCAODC_03885 0.41 -0.2 784,059 - thiF MCAODC_03885 0.41 -2.4 784,059 - thiF MCAODC_03885 0.41 -1.5 784,059 - thiF MCAODC_03885 0.41 -0.1 784,059 - thiF MCAODC_03885 0.41 -1.2 784,059 - thiF MCAODC_03885 0.41 -0.6 784,059 - thiF MCAODC_03885 0.41 -3.5 784,085 + thiF MCAODC_03885 0.44 -0.6 784,085 + thiF MCAODC_03885 0.44 -4.0 784,086 - thiF MCAODC_03885 0.44 -2.7 784,135 - thiF MCAODC_03885 0.51 +0.1 784,135 - thiF MCAODC_03885 0.51 -0.3 784,135 - thiF MCAODC_03885 0.51 -3.0 784,155 + thiF MCAODC_03885 0.53 -4.2 784,188 + thiF MCAODC_03885 0.58 -0.8 784,229 + thiF MCAODC_03885 0.63 -2.8 784,230 - thiF MCAODC_03885 0.63 +1.0 784,230 - thiF MCAODC_03885 0.63 -0.7 784,368 + thiF MCAODC_03885 0.82 -0.1 784,373 + thiF MCAODC_03885 0.82 -2.0 784,373 + thiF MCAODC_03885 0.82 -1.1 784,384 - thiF MCAODC_03885 0.84 -0.9 784,385 + thiF MCAODC_03885 0.84 -1.4 784,386 - thiF MCAODC_03885 0.84 -1.2 784,551 + thiS MCAODC_03890 0.30 -3.0 784,552 - thiS MCAODC_03890 0.31 -1.0 784,552 - thiS MCAODC_03890 0.31 -1.0 784,636 + thiS MCAODC_03890 0.73 -2.4 784,636 + thiS MCAODC_03890 0.73 -2.9 784,636 + thiS MCAODC_03890 0.73 +1.1 784,636 + thiS MCAODC_03890 0.73 -0.2 784,636 + thiS MCAODC_03890 0.73 -0.0 784,637 - thiS MCAODC_03890 0.73 -2.6 784,663 - thiS MCAODC_03890 0.86 +0.7 784,696 + -0.8 784,696 + +0.2 784,697 - -1.6 784,697 - -0.6 784,700 + -1.0 784,700 + -1.3 784,700 + -1.5 784,771 + thiG MCAODC_03895 0.10 -4.8 784,772 - thiG MCAODC_03895 0.10 -2.0 784,895 + thiG MCAODC_03895 0.26 -1.6 785,102 + thiG MCAODC_03895 0.53 -1.1 785,198 + thiG MCAODC_03895 0.66 -1.7 785,201 + thiG MCAODC_03895 0.66 -3.0 785,296 + thiG MCAODC_03895 0.78 -2.9 785,410 - -1.0 785,410 - -3.8
Or see this region's nucleotide sequence