Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt purD and zraR overlap by 4 nucleotides zraR and zraS overlap by 4 nucleotides
MCAODC_03815: purD - Phosphoribosylamine--glycine ligase, at 771,417 to 772,706
purD
MCAODC_03820: zraR - sigma-54-dependent response regulator transcription factor ZraR, at 772,703 to 774,028
zraR
MCAODC_03825: zraS - two-component system sensor histidine kinase ZraS, at 774,025 to 775,401
zraS
Position (kb) 772
773
774
775 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 771.737 kb on + strand, within purD at 771.738 kb on - strand, within purD at 771.761 kb on + strand, within purD at 771.762 kb on - strand, within purD at 771.816 kb on + strand, within purD at 772.008 kb on - strand, within purD at 772.082 kb on + strand, within purD at 772.113 kb on + strand, within purD at 772.114 kb on - strand, within purD at 772.114 kb on - strand, within purD at 772.114 kb on - strand, within purD at 772.166 kb on + strand, within purD at 772.166 kb on + strand, within purD at 772.166 kb on + strand, within purD at 772.166 kb on + strand, within purD at 772.166 kb on + strand, within purD at 772.167 kb on - strand, within purD at 772.167 kb on - strand, within purD at 772.167 kb on - strand, within purD at 772.167 kb on - strand, within purD at 772.212 kb on + strand, within purD at 772.212 kb on + strand, within purD at 772.212 kb on + strand, within purD at 772.227 kb on + strand, within purD at 772.278 kb on - strand, within purD at 772.314 kb on - strand, within purD at 772.372 kb on + strand, within purD at 772.621 kb on - strand at 772.621 kb on - strand at 772.627 kb on + strand at 772.704 kb on + strand at 772.705 kb on - strand at 772.900 kb on + strand, within zraR at 772.901 kb on - strand, within zraR at 772.901 kb on - strand, within zraR at 772.901 kb on - strand, within zraR at 772.901 kb on - strand, within zraR at 772.901 kb on - strand, within zraR at 772.901 kb on - strand, within zraR at 772.958 kb on - strand, within zraR at 772.974 kb on + strand, within zraR at 772.975 kb on - strand, within zraR at 773.006 kb on - strand, within zraR at 773.006 kb on - strand, within zraR at 773.015 kb on + strand, within zraR at 773.016 kb on - strand, within zraR at 773.029 kb on + strand, within zraR at 773.030 kb on - strand, within zraR at 773.030 kb on - strand, within zraR at 773.075 kb on - strand, within zraR at 773.075 kb on - strand, within zraR at 773.102 kb on + strand, within zraR at 773.130 kb on + strand, within zraR at 773.134 kb on - strand, within zraR at 773.218 kb on + strand, within zraR at 773.650 kb on + strand, within zraR at 773.650 kb on + strand, within zraR at 773.651 kb on - strand, within zraR at 773.651 kb on - strand, within zraR at 773.660 kb on - strand, within zraR at 773.722 kb on - strand, within zraR at 773.772 kb on + strand, within zraR at 773.772 kb on + strand, within zraR at 773.772 kb on + strand, within zraR at 773.782 kb on + strand, within zraR at 773.782 kb on + strand, within zraR at 773.782 kb on + strand, within zraR at 773.783 kb on - strand, within zraR at 773.826 kb on - strand, within zraR at 773.826 kb on - strand, within zraR at 773.826 kb on - strand, within zraR at 773.940 kb on + strand at 773.940 kb on + strand at 773.941 kb on - strand at 773.972 kb on - strand at 773.972 kb on - strand at 773.972 kb on - strand at 774.008 kb on - strand at 774.013 kb on + strand at 774.014 kb on - strand at 774.017 kb on - strand at 774.017 kb on - strand at 774.043 kb on - strand at 774.048 kb on + strand at 774.051 kb on + strand at 774.052 kb on - strand at 774.052 kb on - strand at 774.114 kb on + strand at 774.114 kb on + strand at 774.114 kb on + strand at 774.115 kb on - strand at 774.115 kb on - strand at 774.135 kb on + strand at 774.135 kb on + strand at 774.135 kb on + strand at 774.135 kb on + strand at 774.135 kb on + strand at 774.136 kb on - strand at 774.138 kb on + strand at 774.138 kb on + strand at 774.138 kb on + strand at 774.138 kb on + strand at 774.139 kb on - strand at 774.139 kb on - strand at 774.139 kb on - strand at 774.139 kb on - strand at 774.188 kb on + strand, within zraS at 774.189 kb on - strand, within zraS at 774.288 kb on + strand, within zraS at 774.319 kb on - strand, within zraS at 774.319 kb on - strand, within zraS at 774.319 kb on - strand, within zraS at 774.439 kb on + strand, within zraS at 774.440 kb on - strand, within zraS at 774.449 kb on - strand, within zraS at 774.508 kb on - strand, within zraS at 774.727 kb on + strand, within zraS at 774.775 kb on + strand, within zraS at 774.776 kb on - strand, within zraS at 774.813 kb on + strand, within zraS at 774.856 kb on - strand, within zraS at 774.858 kb on + strand, within zraS at 774.858 kb on + strand, within zraS at 774.859 kb on - strand, within zraS at 774.913 kb on - strand, within zraS at 774.913 kb on - strand, within zraS
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 771,737 + purD MCAODC_03815 0.25 -4.6 771,738 - purD MCAODC_03815 0.25 -3.8 771,761 + purD MCAODC_03815 0.27 -6.0 771,762 - purD MCAODC_03815 0.27 -1.2 771,816 + purD MCAODC_03815 0.31 -3.9 772,008 - purD MCAODC_03815 0.46 -2.0 772,082 + purD MCAODC_03815 0.52 -5.7 772,113 + purD MCAODC_03815 0.54 -4.9 772,114 - purD MCAODC_03815 0.54 -0.4 772,114 - purD MCAODC_03815 0.54 -3.7 772,114 - purD MCAODC_03815 0.54 -3.9 772,166 + purD MCAODC_03815 0.58 -3.5 772,166 + purD MCAODC_03815 0.58 -2.9 772,166 + purD MCAODC_03815 0.58 -4.5 772,166 + purD MCAODC_03815 0.58 -4.8 772,166 + purD MCAODC_03815 0.58 -5.2 772,167 - purD MCAODC_03815 0.58 -1.8 772,167 - purD MCAODC_03815 0.58 -3.3 772,167 - purD MCAODC_03815 0.58 -6.0 772,167 - purD MCAODC_03815 0.58 -2.3 772,212 + purD MCAODC_03815 0.62 -0.1 772,212 + purD MCAODC_03815 0.62 -1.5 772,212 + purD MCAODC_03815 0.62 -3.8 772,227 + purD MCAODC_03815 0.63 -3.8 772,278 - purD MCAODC_03815 0.67 -2.2 772,314 - purD MCAODC_03815 0.70 -2.0 772,372 + purD MCAODC_03815 0.74 -3.4 772,621 - -5.0 772,621 - -2.4 772,627 + -3.2 772,704 + +1.0 772,705 - +0.3 772,900 + zraR MCAODC_03820 0.15 -1.4 772,901 - zraR MCAODC_03820 0.15 -0.3 772,901 - zraR MCAODC_03820 0.15 +0.1 772,901 - zraR MCAODC_03820 0.15 +0.1 772,901 - zraR MCAODC_03820 0.15 +0.5 772,901 - zraR MCAODC_03820 0.15 +2.3 772,901 - zraR MCAODC_03820 0.15 -4.0 772,958 - zraR MCAODC_03820 0.19 +0.6 772,974 + zraR MCAODC_03820 0.20 -2.7 772,975 - zraR MCAODC_03820 0.21 -1.8 773,006 - zraR MCAODC_03820 0.23 -0.3 773,006 - zraR MCAODC_03820 0.23 +0.6 773,015 + zraR MCAODC_03820 0.24 +0.8 773,016 - zraR MCAODC_03820 0.24 -0.4 773,029 + zraR MCAODC_03820 0.25 -1.0 773,030 - zraR MCAODC_03820 0.25 +0.8 773,030 - zraR MCAODC_03820 0.25 +0.4 773,075 - zraR MCAODC_03820 0.28 -3.9 773,075 - zraR MCAODC_03820 0.28 -1.6 773,102 + zraR MCAODC_03820 0.30 -1.8 773,130 + zraR MCAODC_03820 0.32 +1.0 773,134 - zraR MCAODC_03820 0.33 -0.3 773,218 + zraR MCAODC_03820 0.39 +1.4 773,650 + zraR MCAODC_03820 0.71 -1.0 773,650 + zraR MCAODC_03820 0.71 +0.1 773,651 - zraR MCAODC_03820 0.71 -1.8 773,651 - zraR MCAODC_03820 0.71 -0.2 773,660 - zraR MCAODC_03820 0.72 -2.9 773,722 - zraR MCAODC_03820 0.77 +0.3 773,772 + zraR MCAODC_03820 0.81 -1.0 773,772 + zraR MCAODC_03820 0.81 +0.4 773,772 + zraR MCAODC_03820 0.81 +0.2 773,782 + zraR MCAODC_03820 0.81 +1.6 773,782 + zraR MCAODC_03820 0.81 -0.3 773,782 + zraR MCAODC_03820 0.81 +0.4 773,783 - zraR MCAODC_03820 0.81 -0.1 773,826 - zraR MCAODC_03820 0.85 +0.3 773,826 - zraR MCAODC_03820 0.85 -0.4 773,826 - zraR MCAODC_03820 0.85 +0.7 773,940 + -0.0 773,940 + -1.7 773,941 - -0.1 773,972 - -2.3 773,972 - -0.9 773,972 - +1.1 774,008 - +0.8 774,013 + -1.8 774,014 - -4.4 774,017 - +0.3 774,017 - -1.8 774,043 - -0.3 774,048 + -0.7 774,051 + +1.6 774,052 - -0.1 774,052 - -0.2 774,114 + -0.4 774,114 + +1.8 774,114 + -0.9 774,115 - +0.5 774,115 - +0.6 774,135 + -0.0 774,135 + +2.5 774,135 + +0.5 774,135 + -1.1 774,135 + -0.5 774,136 - -1.0 774,138 + -1.8 774,138 + +1.1 774,138 + +1.1 774,138 + +0.6 774,139 - +0.8 774,139 - +1.6 774,139 - -0.2 774,139 - -0.8 774,188 + zraS MCAODC_03825 0.12 +1.7 774,189 - zraS MCAODC_03825 0.12 +0.7 774,288 + zraS MCAODC_03825 0.19 +0.5 774,319 - zraS MCAODC_03825 0.21 -1.9 774,319 - zraS MCAODC_03825 0.21 +1.4 774,319 - zraS MCAODC_03825 0.21 +0.4 774,439 + zraS MCAODC_03825 0.30 -0.2 774,440 - zraS MCAODC_03825 0.30 +0.3 774,449 - zraS MCAODC_03825 0.31 -0.5 774,508 - zraS MCAODC_03825 0.35 -2.9 774,727 + zraS MCAODC_03825 0.51 +0.8 774,775 + zraS MCAODC_03825 0.54 -1.5 774,776 - zraS MCAODC_03825 0.55 -3.2 774,813 + zraS MCAODC_03825 0.57 +0.6 774,856 - zraS MCAODC_03825 0.60 +0.7 774,858 + zraS MCAODC_03825 0.60 +0.5 774,858 + zraS MCAODC_03825 0.60 +0.4 774,859 - zraS MCAODC_03825 0.61 +0.4 774,913 - zraS MCAODC_03825 0.64 -0.0 774,913 - zraS MCAODC_03825 0.64 +0.6
Or see this region's nucleotide sequence