Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt uvrA and yjbR are separated by 34 nucleotides yjbR and yjbQ are separated by 3 nucleotides yjbQ and aphA are separated by 110 nucleotides
MCAODC_03405: uvrA - excinuclease ABC subunit UvrA, at 687,047 to 689,869
uvrA
MCAODC_03410: yjbR - Uncharacterized protein YjbR, at 689,904 to 690,260
yjbR
MCAODC_03415: yjbQ - UPF0047 protein YjbQ, at 690,264 to 690,680
yjbQ
MCAODC_03420: aphA - acid phosphatase AphA, at 690,791 to 691,504
aphA
Position (kb) 689
690
691 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 688.930 kb on - strand, within uvrA at 688.952 kb on - strand, within uvrA at 689.132 kb on - strand, within uvrA at 689.156 kb on + strand, within uvrA at 689.156 kb on + strand, within uvrA at 689.156 kb on + strand, within uvrA at 689.157 kb on - strand, within uvrA at 689.157 kb on - strand, within uvrA at 689.157 kb on - strand, within uvrA at 689.157 kb on - strand, within uvrA at 689.157 kb on - strand, within uvrA at 689.222 kb on + strand, within uvrA at 689.222 kb on + strand, within uvrA at 689.224 kb on + strand, within uvrA at 689.224 kb on + strand, within uvrA at 689.224 kb on + strand, within uvrA at 689.224 kb on + strand, within uvrA at 689.225 kb on - strand, within uvrA at 689.246 kb on - strand, within uvrA at 689.324 kb on + strand, within uvrA at 689.324 kb on + strand, within uvrA at 689.324 kb on + strand, within uvrA at 689.324 kb on + strand, within uvrA at 689.324 kb on + strand, within uvrA at 689.351 kb on - strand, within uvrA at 689.351 kb on - strand, within uvrA at 689.357 kb on + strand, within uvrA at 689.357 kb on + strand, within uvrA at 689.358 kb on - strand, within uvrA at 689.358 kb on - strand, within uvrA at 689.358 kb on - strand, within uvrA at 689.381 kb on - strand, within uvrA at 689.384 kb on + strand, within uvrA at 689.384 kb on + strand, within uvrA at 689.385 kb on - strand, within uvrA at 689.385 kb on - strand, within uvrA at 689.417 kb on - strand, within uvrA at 689.504 kb on + strand, within uvrA at 689.505 kb on - strand, within uvrA at 689.606 kb on + strand at 689.606 kb on + strand at 689.606 kb on + strand at 689.606 kb on + strand at 689.607 kb on - strand at 689.607 kb on - strand at 689.608 kb on + strand at 689.608 kb on + strand at 689.608 kb on + strand at 689.609 kb on - strand at 689.674 kb on + strand at 689.731 kb on + strand at 689.731 kb on + strand at 689.839 kb on + strand at 689.840 kb on - strand at 689.924 kb on + strand at 689.943 kb on + strand, within yjbR at 689.944 kb on - strand, within yjbR at 689.962 kb on + strand, within yjbR at 689.981 kb on - strand, within yjbR at 689.981 kb on - strand, within yjbR at 689.983 kb on + strand, within yjbR at 689.984 kb on - strand, within yjbR at 689.984 kb on - strand, within yjbR at 690.017 kb on - strand, within yjbR at 690.017 kb on - strand, within yjbR at 690.017 kb on - strand, within yjbR at 690.038 kb on + strand, within yjbR at 690.039 kb on - strand, within yjbR at 690.136 kb on - strand, within yjbR at 690.136 kb on - strand, within yjbR at 690.159 kb on + strand, within yjbR at 690.160 kb on - strand, within yjbR at 690.160 kb on - strand, within yjbR at 690.160 kb on - strand, within yjbR at 690.160 kb on - strand, within yjbR at 690.198 kb on - strand, within yjbR at 690.235 kb on + strand at 690.240 kb on + strand at 690.240 kb on + strand at 690.240 kb on + strand at 690.240 kb on + strand at 690.240 kb on + strand at 690.240 kb on + strand at 690.240 kb on + strand at 690.240 kb on + strand at 690.240 kb on + strand at 690.241 kb on - strand at 690.241 kb on - strand at 690.241 kb on - strand at 690.276 kb on + strand at 690.365 kb on + strand, within yjbQ at 690.378 kb on + strand, within yjbQ at 690.378 kb on + strand, within yjbQ at 690.378 kb on + strand, within yjbQ at 690.378 kb on + strand, within yjbQ at 690.379 kb on - strand, within yjbQ at 690.379 kb on - strand, within yjbQ at 690.411 kb on - strand, within yjbQ at 690.411 kb on - strand, within yjbQ at 690.422 kb on + strand, within yjbQ at 690.439 kb on + strand, within yjbQ at 690.439 kb on + strand, within yjbQ at 690.439 kb on + strand, within yjbQ at 690.451 kb on + strand, within yjbQ at 690.452 kb on - strand, within yjbQ at 690.504 kb on + strand, within yjbQ at 690.505 kb on - strand, within yjbQ at 690.527 kb on + strand, within yjbQ at 690.528 kb on - strand, within yjbQ at 690.528 kb on - strand, within yjbQ at 690.528 kb on - strand, within yjbQ at 690.528 kb on - strand, within yjbQ at 690.548 kb on + strand, within yjbQ at 690.548 kb on + strand, within yjbQ at 690.548 kb on + strand, within yjbQ at 690.549 kb on - strand, within yjbQ at 690.549 kb on - strand, within yjbQ at 690.549 kb on - strand, within yjbQ at 690.549 kb on - strand, within yjbQ at 690.570 kb on + strand, within yjbQ at 690.593 kb on + strand, within yjbQ at 690.674 kb on - strand at 690.696 kb on - strand at 690.853 kb on - strand at 690.853 kb on - strand at 690.857 kb on - strand at 690.857 kb on - strand at 690.917 kb on - strand, within aphA at 690.917 kb on - strand, within aphA at 691.124 kb on + strand, within aphA at 691.124 kb on + strand, within aphA at 691.125 kb on - strand, within aphA at 691.125 kb on - strand, within aphA at 691.125 kb on - strand, within aphA at 691.125 kb on - strand, within aphA at 691.125 kb on - strand, within aphA at 691.125 kb on - strand, within aphA at 691.132 kb on + strand, within aphA at 691.132 kb on + strand, within aphA at 691.133 kb on - strand, within aphA at 691.205 kb on + strand, within aphA at 691.221 kb on + strand, within aphA at 691.221 kb on + strand, within aphA at 691.222 kb on - strand, within aphA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 688,930 - uvrA MCAODC_03405 0.67 -3.7 688,952 - uvrA MCAODC_03405 0.67 +0.8 689,132 - uvrA MCAODC_03405 0.74 -0.4 689,156 + uvrA MCAODC_03405 0.75 -3.1 689,156 + uvrA MCAODC_03405 0.75 -1.6 689,156 + uvrA MCAODC_03405 0.75 +0.3 689,157 - uvrA MCAODC_03405 0.75 +1.4 689,157 - uvrA MCAODC_03405 0.75 +3.0 689,157 - uvrA MCAODC_03405 0.75 -2.5 689,157 - uvrA MCAODC_03405 0.75 -2.3 689,157 - uvrA MCAODC_03405 0.75 -0.6 689,222 + uvrA MCAODC_03405 0.77 +1.0 689,222 + uvrA MCAODC_03405 0.77 +1.2 689,224 + uvrA MCAODC_03405 0.77 +2.4 689,224 + uvrA MCAODC_03405 0.77 +0.9 689,224 + uvrA MCAODC_03405 0.77 -0.3 689,224 + uvrA MCAODC_03405 0.77 -0.6 689,225 - uvrA MCAODC_03405 0.77 +0.8 689,246 - uvrA MCAODC_03405 0.78 -1.9 689,324 + uvrA MCAODC_03405 0.81 -1.2 689,324 + uvrA MCAODC_03405 0.81 -0.6 689,324 + uvrA MCAODC_03405 0.81 -2.0 689,324 + uvrA MCAODC_03405 0.81 -0.4 689,324 + uvrA MCAODC_03405 0.81 +0.2 689,351 - uvrA MCAODC_03405 0.82 -0.4 689,351 - uvrA MCAODC_03405 0.82 +1.3 689,357 + uvrA MCAODC_03405 0.82 +2.1 689,357 + uvrA MCAODC_03405 0.82 -0.2 689,358 - uvrA MCAODC_03405 0.82 -2.6 689,358 - uvrA MCAODC_03405 0.82 -0.7 689,358 - uvrA MCAODC_03405 0.82 +1.8 689,381 - uvrA MCAODC_03405 0.83 +0.6 689,384 + uvrA MCAODC_03405 0.83 +0.4 689,384 + uvrA MCAODC_03405 0.83 +0.6 689,385 - uvrA MCAODC_03405 0.83 -0.7 689,385 - uvrA MCAODC_03405 0.83 -2.5 689,417 - uvrA MCAODC_03405 0.84 +1.3 689,504 + uvrA MCAODC_03405 0.87 -3.1 689,505 - uvrA MCAODC_03405 0.87 +0.7 689,606 + +0.5 689,606 + +1.9 689,606 + +0.6 689,606 + -0.6 689,607 - -1.5 689,607 - +2.5 689,608 + -3.9 689,608 + -3.4 689,608 + +0.5 689,609 - +0.1 689,674 + +0.3 689,731 + -1.9 689,731 + -2.3 689,839 + +2.9 689,840 - +1.1 689,924 + +1.4 689,943 + yjbR MCAODC_03410 0.11 +0.9 689,944 - yjbR MCAODC_03410 0.11 +2.3 689,962 + yjbR MCAODC_03410 0.16 +0.4 689,981 - yjbR MCAODC_03410 0.22 +1.1 689,981 - yjbR MCAODC_03410 0.22 +2.5 689,983 + yjbR MCAODC_03410 0.22 +1.3 689,984 - yjbR MCAODC_03410 0.22 +1.1 689,984 - yjbR MCAODC_03410 0.22 +0.9 690,017 - yjbR MCAODC_03410 0.32 -1.9 690,017 - yjbR MCAODC_03410 0.32 +0.9 690,017 - yjbR MCAODC_03410 0.32 +1.2 690,038 + yjbR MCAODC_03410 0.38 +0.4 690,039 - yjbR MCAODC_03410 0.38 +0.5 690,136 - yjbR MCAODC_03410 0.65 -0.5 690,136 - yjbR MCAODC_03410 0.65 +1.0 690,159 + yjbR MCAODC_03410 0.71 +1.6 690,160 - yjbR MCAODC_03410 0.72 +0.5 690,160 - yjbR MCAODC_03410 0.72 +0.9 690,160 - yjbR MCAODC_03410 0.72 +0.2 690,160 - yjbR MCAODC_03410 0.72 -0.0 690,198 - yjbR MCAODC_03410 0.82 +0.6 690,235 + -2.3 690,240 + -0.6 690,240 + -0.5 690,240 + +1.0 690,240 + +0.4 690,240 + -2.3 690,240 + +0.5 690,240 + +1.4 690,240 + +0.5 690,240 + +1.0 690,241 - -1.0 690,241 - -1.2 690,241 - +0.4 690,276 + +1.7 690,365 + yjbQ MCAODC_03415 0.24 +0.4 690,378 + yjbQ MCAODC_03415 0.27 -0.2 690,378 + yjbQ MCAODC_03415 0.27 +0.0 690,378 + yjbQ MCAODC_03415 0.27 +0.9 690,378 + yjbQ MCAODC_03415 0.27 +0.7 690,379 - yjbQ MCAODC_03415 0.28 -1.5 690,379 - yjbQ MCAODC_03415 0.28 +0.1 690,411 - yjbQ MCAODC_03415 0.35 +0.5 690,411 - yjbQ MCAODC_03415 0.35 +1.8 690,422 + yjbQ MCAODC_03415 0.38 +0.2 690,439 + yjbQ MCAODC_03415 0.42 +0.6 690,439 + yjbQ MCAODC_03415 0.42 -0.1 690,439 + yjbQ MCAODC_03415 0.42 +0.2 690,451 + yjbQ MCAODC_03415 0.45 +0.1 690,452 - yjbQ MCAODC_03415 0.45 +0.8 690,504 + yjbQ MCAODC_03415 0.58 +0.2 690,505 - yjbQ MCAODC_03415 0.58 -2.1 690,527 + yjbQ MCAODC_03415 0.63 +0.7 690,528 - yjbQ MCAODC_03415 0.63 +2.5 690,528 - yjbQ MCAODC_03415 0.63 -0.1 690,528 - yjbQ MCAODC_03415 0.63 -0.5 690,528 - yjbQ MCAODC_03415 0.63 +0.2 690,548 + yjbQ MCAODC_03415 0.68 -0.2 690,548 + yjbQ MCAODC_03415 0.68 -0.8 690,548 + yjbQ MCAODC_03415 0.68 +0.3 690,549 - yjbQ MCAODC_03415 0.68 -2.3 690,549 - yjbQ MCAODC_03415 0.68 -1.4 690,549 - yjbQ MCAODC_03415 0.68 +1.7 690,549 - yjbQ MCAODC_03415 0.68 -0.6 690,570 + yjbQ MCAODC_03415 0.73 -2.6 690,593 + yjbQ MCAODC_03415 0.79 -1.6 690,674 - -0.0 690,696 - +0.5 690,853 - -1.2 690,853 - +1.1 690,857 - -2.0 690,857 - +0.2 690,917 - aphA MCAODC_03420 0.18 +1.7 690,917 - aphA MCAODC_03420 0.18 -1.5 691,124 + aphA MCAODC_03420 0.47 +1.5 691,124 + aphA MCAODC_03420 0.47 -0.7 691,125 - aphA MCAODC_03420 0.47 -1.1 691,125 - aphA MCAODC_03420 0.47 +0.9 691,125 - aphA MCAODC_03420 0.47 +0.5 691,125 - aphA MCAODC_03420 0.47 +1.2 691,125 - aphA MCAODC_03420 0.47 +1.4 691,125 - aphA MCAODC_03420 0.47 -1.3 691,132 + aphA MCAODC_03420 0.48 -0.4 691,132 + aphA MCAODC_03420 0.48 -0.0 691,133 - aphA MCAODC_03420 0.48 +1.7 691,205 + aphA MCAODC_03420 0.58 +0.7 691,221 + aphA MCAODC_03420 0.60 -0.2 691,221 + aphA MCAODC_03420 0.60 -0.4 691,222 - aphA MCAODC_03420 0.60 -4.1
Or see this region's nucleotide sequence