Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt phnO and phnP are separated by 1 nucleotides phnP and yjdP are separated by 76 nucleotides yjdP and MCAODC_03215 are separated by 68 nucleotides
MCAODC_03200: phnO - aminoalkylphosphonate N-acetyltransferase, at 643,682 to 644,107
phnO
MCAODC_03205: phnP - phosphonate metabolism protein PhnP, at 644,109 to 644,867
phnP
MCAODC_03210: yjdP - Uncharacterized protein YjdP, at 644,944 to 645,273
yjdP
MCAODC_03215: MCAODC_03215 - hybrid sensor histidine kinase/response regulator, at 645,342 to 647,612
_03215
Position (kb) 644
645
646 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 643.957 kb on + strand, within phnO at 643.957 kb on + strand, within phnO at 643.963 kb on - strand, within phnO at 644.055 kb on - strand, within phnO at 644.105 kb on + strand at 644.105 kb on + strand at 644.105 kb on + strand at 644.105 kb on + strand at 644.105 kb on + strand at 644.106 kb on - strand at 644.106 kb on - strand at 644.106 kb on - strand at 644.106 kb on - strand at 644.246 kb on + strand, within phnP at 644.342 kb on + strand, within phnP at 644.342 kb on + strand, within phnP at 644.343 kb on - strand, within phnP at 644.343 kb on - strand, within phnP at 644.343 kb on - strand, within phnP at 644.510 kb on - strand, within phnP at 644.657 kb on - strand, within phnP at 644.716 kb on + strand, within phnP at 644.716 kb on + strand, within phnP at 644.716 kb on + strand, within phnP at 644.717 kb on - strand, within phnP at 644.954 kb on + strand at 644.954 kb on + strand at 644.955 kb on - strand at 644.986 kb on + strand, within yjdP at 644.986 kb on + strand, within yjdP at 644.987 kb on - strand, within yjdP at 645.007 kb on - strand, within yjdP at 645.071 kb on + strand, within yjdP at 645.071 kb on + strand, within yjdP at 645.071 kb on + strand, within yjdP at 645.072 kb on - strand, within yjdP at 645.072 kb on - strand, within yjdP at 645.072 kb on - strand, within yjdP at 645.153 kb on + strand, within yjdP at 645.154 kb on - strand, within yjdP at 645.154 kb on - strand, within yjdP at 645.154 kb on - strand, within yjdP at 645.180 kb on + strand, within yjdP at 645.181 kb on - strand, within yjdP at 645.202 kb on - strand, within yjdP at 645.296 kb on + strand at 645.297 kb on - strand at 645.299 kb on + strand at 645.299 kb on + strand at 645.299 kb on + strand at 645.299 kb on + strand at 645.299 kb on + strand at 645.299 kb on + strand at 645.300 kb on - strand at 645.300 kb on - strand at 645.323 kb on + strand at 645.459 kb on + strand at 645.460 kb on - strand at 645.535 kb on - strand at 645.546 kb on - strand at 645.620 kb on - strand, within MCAODC_03215 at 645.681 kb on + strand, within MCAODC_03215 at 645.682 kb on - strand, within MCAODC_03215 at 645.682 kb on - strand, within MCAODC_03215 at 645.682 kb on - strand, within MCAODC_03215 at 645.682 kb on - strand, within MCAODC_03215 at 645.717 kb on + strand, within MCAODC_03215 at 645.841 kb on + strand, within MCAODC_03215 at 645.841 kb on + strand, within MCAODC_03215 at 645.841 kb on + strand, within MCAODC_03215 at 645.890 kb on + strand, within MCAODC_03215 at 645.907 kb on - strand, within MCAODC_03215 at 645.932 kb on + strand, within MCAODC_03215 at 646.176 kb on + strand, within MCAODC_03215 at 646.177 kb on - strand, within MCAODC_03215 at 646.185 kb on + strand, within MCAODC_03215 at 646.227 kb on + strand, within MCAODC_03215 at 646.228 kb on - strand, within MCAODC_03215 at 646.228 kb on - strand, within MCAODC_03215
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 643,957 + phnO MCAODC_03200 0.65 -0.7 643,957 + phnO MCAODC_03200 0.65 +0.7 643,963 - phnO MCAODC_03200 0.66 +0.3 644,055 - phnO MCAODC_03200 0.88 +2.2 644,105 + +0.9 644,105 + +0.9 644,105 + +2.9 644,105 + +0.4 644,105 + -0.5 644,106 - +0.3 644,106 - -1.5 644,106 - -0.9 644,106 - -1.4 644,246 + phnP MCAODC_03205 0.18 -2.0 644,342 + phnP MCAODC_03205 0.31 +2.3 644,342 + phnP MCAODC_03205 0.31 -1.9 644,343 - phnP MCAODC_03205 0.31 +1.3 644,343 - phnP MCAODC_03205 0.31 -0.3 644,343 - phnP MCAODC_03205 0.31 +0.1 644,510 - phnP MCAODC_03205 0.53 +0.5 644,657 - phnP MCAODC_03205 0.72 -0.6 644,716 + phnP MCAODC_03205 0.80 -2.7 644,716 + phnP MCAODC_03205 0.80 +0.9 644,716 + phnP MCAODC_03205 0.80 +1.0 644,717 - phnP MCAODC_03205 0.80 +1.1 644,954 + +1.0 644,954 + -2.5 644,955 - -1.0 644,986 + yjdP MCAODC_03210 0.13 +0.7 644,986 + yjdP MCAODC_03210 0.13 +0.0 644,987 - yjdP MCAODC_03210 0.13 +1.0 645,007 - yjdP MCAODC_03210 0.19 -0.8 645,071 + yjdP MCAODC_03210 0.38 +0.4 645,071 + yjdP MCAODC_03210 0.38 +2.0 645,071 + yjdP MCAODC_03210 0.38 -1.9 645,072 - yjdP MCAODC_03210 0.39 -0.7 645,072 - yjdP MCAODC_03210 0.39 +0.2 645,072 - yjdP MCAODC_03210 0.39 -2.8 645,153 + yjdP MCAODC_03210 0.63 +0.3 645,154 - yjdP MCAODC_03210 0.64 -3.8 645,154 - yjdP MCAODC_03210 0.64 +0.4 645,154 - yjdP MCAODC_03210 0.64 -0.7 645,180 + yjdP MCAODC_03210 0.72 +0.6 645,181 - yjdP MCAODC_03210 0.72 +1.4 645,202 - yjdP MCAODC_03210 0.78 -3.8 645,296 + -0.7 645,297 - +1.3 645,299 + -1.5 645,299 + +0.5 645,299 + +0.4 645,299 + +0.1 645,299 + +0.6 645,299 + -0.1 645,300 - +0.7 645,300 - +0.9 645,323 + +0.2 645,459 + +0.6 645,460 - -1.2 645,535 - -1.9 645,546 - -1.5 645,620 - MCAODC_03215 0.12 -0.4 645,681 + MCAODC_03215 0.15 -1.2 645,682 - MCAODC_03215 0.15 +0.8 645,682 - MCAODC_03215 0.15 +0.7 645,682 - MCAODC_03215 0.15 +1.0 645,682 - MCAODC_03215 0.15 +1.1 645,717 + MCAODC_03215 0.17 -3.2 645,841 + MCAODC_03215 0.22 +0.7 645,841 + MCAODC_03215 0.22 -1.3 645,841 + MCAODC_03215 0.22 +1.4 645,890 + MCAODC_03215 0.24 -3.7 645,907 - MCAODC_03215 0.25 -0.4 645,932 + MCAODC_03215 0.26 +0.0 646,176 + MCAODC_03215 0.37 +0.4 646,177 - MCAODC_03215 0.37 -0.2 646,185 + MCAODC_03215 0.37 +1.9 646,227 + MCAODC_03215 0.39 +0.1 646,228 - MCAODC_03215 0.39 -0.9 646,228 - MCAODC_03215 0.39 +0.9
Or see this region's nucleotide sequence