Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pmbA and yjgA are separated by 93 nucleotides yjgA and mpl are separated by 155 nucleotides mpl and fbp are separated by 175 nucleotides
MCAODC_02430: pmbA - metalloprotease PmbA, at 499,291 to 500,643
pmbA
MCAODC_02435: yjgA - ribosome-associated protein, at 500,737 to 501,288
yjgA
MCAODC_02445: mpl - UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-me so-diaminopimelate ligase, at 501,444 to 502,817
mpl
MCAODC_02450: fbp - class 1 fructose-bisphosphatase, at 502,993 to 503,991
fbp
Position (kb) 501
502
503 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 500.490 kb on - strand, within pmbA at 500.501 kb on - strand, within pmbA at 500.501 kb on - strand, within pmbA at 500.506 kb on - strand, within pmbA at 500.623 kb on + strand at 500.623 kb on + strand at 500.624 kb on - strand at 500.624 kb on - strand at 500.643 kb on + strand at 500.643 kb on + strand at 500.644 kb on - strand at 500.648 kb on + strand at 500.649 kb on - strand at 500.669 kb on - strand at 500.698 kb on + strand at 500.699 kb on - strand at 500.699 kb on - strand at 500.699 kb on - strand at 500.699 kb on - strand at 500.712 kb on - strand at 500.742 kb on + strand at 500.742 kb on + strand at 500.742 kb on + strand at 500.743 kb on - strand at 500.743 kb on - strand at 500.808 kb on + strand, within yjgA at 500.808 kb on + strand, within yjgA at 500.821 kb on - strand, within yjgA at 500.821 kb on - strand, within yjgA at 500.852 kb on + strand, within yjgA at 500.852 kb on + strand, within yjgA at 500.852 kb on + strand, within yjgA at 500.853 kb on - strand, within yjgA at 500.912 kb on + strand, within yjgA at 500.955 kb on + strand, within yjgA at 500.955 kb on + strand, within yjgA at 500.955 kb on + strand, within yjgA at 500.956 kb on - strand, within yjgA at 501.017 kb on + strand, within yjgA at 501.018 kb on - strand, within yjgA at 501.084 kb on + strand, within yjgA at 501.084 kb on + strand, within yjgA at 501.085 kb on - strand, within yjgA at 501.146 kb on + strand, within yjgA at 501.147 kb on - strand, within yjgA at 501.147 kb on - strand, within yjgA at 501.147 kb on - strand, within yjgA at 501.222 kb on + strand, within yjgA at 501.223 kb on - strand, within yjgA at 501.256 kb on + strand at 501.257 kb on - strand at 501.257 kb on - strand at 501.287 kb on - strand at 501.308 kb on + strand at 501.309 kb on - strand at 501.309 kb on - strand at 501.336 kb on - strand at 501.345 kb on - strand at 501.374 kb on - strand at 501.374 kb on - strand at 501.374 kb on - strand at 501.424 kb on + strand at 501.425 kb on - strand at 501.425 kb on - strand at 501.483 kb on + strand at 501.530 kb on + strand at 501.531 kb on - strand at 501.563 kb on + strand at 501.564 kb on - strand at 501.564 kb on - strand at 501.564 kb on - strand at 501.684 kb on + strand, within mpl at 501.684 kb on + strand, within mpl at 501.685 kb on - strand, within mpl at 501.755 kb on + strand, within mpl at 501.755 kb on + strand, within mpl at 501.755 kb on + strand, within mpl at 501.756 kb on - strand, within mpl at 501.838 kb on + strand, within mpl at 501.839 kb on - strand, within mpl at 501.840 kb on + strand, within mpl at 501.840 kb on + strand, within mpl at 501.840 kb on + strand, within mpl at 501.840 kb on + strand, within mpl at 501.840 kb on + strand, within mpl at 501.841 kb on - strand, within mpl at 501.841 kb on - strand, within mpl at 501.841 kb on - strand, within mpl at 501.891 kb on - strand, within mpl at 501.894 kb on - strand, within mpl at 501.977 kb on + strand, within mpl at 501.978 kb on - strand, within mpl at 501.980 kb on + strand, within mpl at 501.981 kb on - strand, within mpl at 501.981 kb on - strand, within mpl at 501.981 kb on - strand, within mpl at 502.167 kb on - strand, within mpl at 502.167 kb on - strand, within mpl at 502.167 kb on - strand, within mpl at 502.169 kb on + strand, within mpl at 502.169 kb on + strand, within mpl at 502.169 kb on + strand, within mpl at 502.170 kb on - strand, within mpl at 502.170 kb on - strand, within mpl at 502.281 kb on - strand, within mpl at 502.333 kb on + strand, within mpl at 502.334 kb on - strand, within mpl at 502.334 kb on - strand, within mpl at 502.414 kb on - strand, within mpl at 502.429 kb on + strand, within mpl at 502.430 kb on - strand, within mpl at 502.553 kb on + strand, within mpl at 502.553 kb on + strand, within mpl at 502.553 kb on + strand, within mpl at 502.553 kb on + strand, within mpl at 502.553 kb on + strand, within mpl at 502.553 kb on + strand, within mpl at 502.553 kb on + strand, within mpl at 502.554 kb on - strand, within mpl at 502.554 kb on - strand, within mpl at 502.555 kb on + strand, within mpl at 502.555 kb on + strand, within mpl at 502.555 kb on + strand, within mpl at 502.555 kb on + strand, within mpl at 502.555 kb on + strand, within mpl at 502.555 kb on + strand, within mpl at 502.555 kb on + strand, within mpl at 502.555 kb on + strand, within mpl at 502.555 kb on + strand, within mpl at 502.555 kb on + strand, within mpl at 502.556 kb on - strand, within mpl at 502.556 kb on - strand, within mpl at 502.556 kb on - strand, within mpl at 502.556 kb on - strand, within mpl at 502.556 kb on - strand, within mpl at 502.556 kb on - strand, within mpl at 502.619 kb on + strand, within mpl at 502.661 kb on - strand, within mpl at 502.661 kb on - strand, within mpl at 502.661 kb on - strand, within mpl at 502.696 kb on - strand at 502.696 kb on - strand at 502.696 kb on - strand at 502.696 kb on - strand at 502.696 kb on - strand at 502.714 kb on + strand at 502.738 kb on - strand at 502.750 kb on - strand at 502.750 kb on - strand at 502.750 kb on - strand at 502.800 kb on + strand at 502.835 kb on + strand at 502.852 kb on + strand at 502.852 kb on + strand at 502.858 kb on + strand at 502.858 kb on + strand at 502.858 kb on + strand at 502.858 kb on + strand at 502.859 kb on - strand at 502.859 kb on - strand at 502.859 kb on - strand at 502.859 kb on - strand at 502.859 kb on - strand at 502.897 kb on + strand at 502.937 kb on + strand at 502.993 kb on - strand at 503.003 kb on + strand at 503.004 kb on - strand at 503.004 kb on - strand at 503.013 kb on + strand at 503.014 kb on - strand at 503.078 kb on + strand at 503.079 kb on - strand at 503.098 kb on - strand, within fbp at 503.235 kb on + strand, within fbp at 503.305 kb on + strand, within fbp at 503.305 kb on + strand, within fbp at 503.306 kb on - strand, within fbp at 503.306 kb on - strand, within fbp at 503.383 kb on + strand, within fbp at 503.384 kb on - strand, within fbp at 503.394 kb on + strand, within fbp at 503.394 kb on + strand, within fbp at 503.395 kb on - strand, within fbp at 503.395 kb on - strand, within fbp at 503.473 kb on + strand, within fbp at 503.473 kb on + strand, within fbp at 503.473 kb on + strand, within fbp at 503.473 kb on + strand, within fbp at 503.497 kb on + strand, within fbp at 503.497 kb on + strand, within fbp at 503.497 kb on + strand, within fbp at 503.526 kb on + strand, within fbp at 503.526 kb on + strand, within fbp at 503.530 kb on + strand, within fbp at 503.530 kb on + strand, within fbp at 503.530 kb on + strand, within fbp at 503.599 kb on + strand, within fbp at 503.599 kb on + strand, within fbp at 503.600 kb on - strand, within fbp at 503.620 kb on + strand, within fbp at 503.620 kb on + strand, within fbp at 503.621 kb on - strand, within fbp at 503.625 kb on + strand, within fbp at 503.625 kb on + strand, within fbp at 503.625 kb on + strand, within fbp at 503.625 kb on + strand, within fbp at 503.625 kb on + strand, within fbp at 503.626 kb on - strand, within fbp at 503.650 kb on + strand, within fbp at 503.650 kb on + strand, within fbp at 503.650 kb on + strand, within fbp at 503.676 kb on + strand, within fbp at 503.698 kb on - strand, within fbp at 503.698 kb on - strand, within fbp at 503.698 kb on - strand, within fbp at 503.698 kb on - strand, within fbp at 503.707 kb on + strand, within fbp at 503.707 kb on + strand, within fbp at 503.708 kb on - strand, within fbp at 503.710 kb on - strand, within fbp at 503.757 kb on + strand, within fbp at 503.813 kb on - strand, within fbp
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 500,490 - pmbA MCAODC_02430 0.89 +0.5 500,501 - pmbA MCAODC_02430 0.89 +0.9 500,501 - pmbA MCAODC_02430 0.89 +0.6 500,506 - pmbA MCAODC_02430 0.90 -1.1 500,623 + +2.1 500,623 + +0.7 500,624 - -1.5 500,624 - +0.2 500,643 + +0.1 500,643 + +0.5 500,644 - +1.0 500,648 + -1.6 500,649 - -2.0 500,669 - -1.3 500,698 + -0.1 500,699 - +1.0 500,699 - +0.9 500,699 - -0.8 500,699 - +1.0 500,712 - -2.3 500,742 + +0.3 500,742 + +0.2 500,742 + +0.8 500,743 - -1.9 500,743 - -1.6 500,808 + yjgA MCAODC_02435 0.13 -1.4 500,808 + yjgA MCAODC_02435 0.13 +0.3 500,821 - yjgA MCAODC_02435 0.15 -1.7 500,821 - yjgA MCAODC_02435 0.15 -2.3 500,852 + yjgA MCAODC_02435 0.21 +0.6 500,852 + yjgA MCAODC_02435 0.21 -0.7 500,852 + yjgA MCAODC_02435 0.21 +0.6 500,853 - yjgA MCAODC_02435 0.21 +0.8 500,912 + yjgA MCAODC_02435 0.32 -0.7 500,955 + yjgA MCAODC_02435 0.39 +0.9 500,955 + yjgA MCAODC_02435 0.39 +1.8 500,955 + yjgA MCAODC_02435 0.39 -0.8 500,956 - yjgA MCAODC_02435 0.40 -1.2 501,017 + yjgA MCAODC_02435 0.51 -0.3 501,018 - yjgA MCAODC_02435 0.51 +1.6 501,084 + yjgA MCAODC_02435 0.63 +0.3 501,084 + yjgA MCAODC_02435 0.63 -0.6 501,085 - yjgA MCAODC_02435 0.63 -1.6 501,146 + yjgA MCAODC_02435 0.74 -0.9 501,147 - yjgA MCAODC_02435 0.74 -3.0 501,147 - yjgA MCAODC_02435 0.74 +1.5 501,147 - yjgA MCAODC_02435 0.74 +1.5 501,222 + yjgA MCAODC_02435 0.88 +1.1 501,223 - yjgA MCAODC_02435 0.88 -0.9 501,256 + -0.1 501,257 - +0.8 501,257 - -1.9 501,287 - -0.4 501,308 + +0.2 501,309 - -1.3 501,309 - +0.5 501,336 - -0.7 501,345 - -0.5 501,374 - -0.2 501,374 - +0.6 501,374 - +0.1 501,424 + -0.9 501,425 - +0.9 501,425 - +0.6 501,483 + +0.1 501,530 + +2.2 501,531 - -0.4 501,563 + +0.6 501,564 - -1.5 501,564 - +0.3 501,564 - -0.8 501,684 + mpl MCAODC_02445 0.17 +0.5 501,684 + mpl MCAODC_02445 0.17 +1.2 501,685 - mpl MCAODC_02445 0.18 -0.2 501,755 + mpl MCAODC_02445 0.23 +0.5 501,755 + mpl MCAODC_02445 0.23 -3.5 501,755 + mpl MCAODC_02445 0.23 -0.3 501,756 - mpl MCAODC_02445 0.23 +0.4 501,838 + mpl MCAODC_02445 0.29 +0.0 501,839 - mpl MCAODC_02445 0.29 +1.2 501,840 + mpl MCAODC_02445 0.29 +0.1 501,840 + mpl MCAODC_02445 0.29 +0.5 501,840 + mpl MCAODC_02445 0.29 -1.9 501,840 + mpl MCAODC_02445 0.29 -2.1 501,840 + mpl MCAODC_02445 0.29 +0.4 501,841 - mpl MCAODC_02445 0.29 -1.3 501,841 - mpl MCAODC_02445 0.29 -0.2 501,841 - mpl MCAODC_02445 0.29 +0.5 501,891 - mpl MCAODC_02445 0.33 -2.4 501,894 - mpl MCAODC_02445 0.33 -0.2 501,977 + mpl MCAODC_02445 0.39 +0.4 501,978 - mpl MCAODC_02445 0.39 +0.9 501,980 + mpl MCAODC_02445 0.39 -0.4 501,981 - mpl MCAODC_02445 0.39 +0.0 501,981 - mpl MCAODC_02445 0.39 -0.2 501,981 - mpl MCAODC_02445 0.39 -1.5 502,167 - mpl MCAODC_02445 0.53 -0.9 502,167 - mpl MCAODC_02445 0.53 +0.6 502,167 - mpl MCAODC_02445 0.53 -3.1 502,169 + mpl MCAODC_02445 0.53 +1.1 502,169 + mpl MCAODC_02445 0.53 +0.4 502,169 + mpl MCAODC_02445 0.53 +0.4 502,170 - mpl MCAODC_02445 0.53 +0.1 502,170 - mpl MCAODC_02445 0.53 -0.1 502,281 - mpl MCAODC_02445 0.61 +0.8 502,333 + mpl MCAODC_02445 0.65 -3.0 502,334 - mpl MCAODC_02445 0.65 +0.9 502,334 - mpl MCAODC_02445 0.65 -0.5 502,414 - mpl MCAODC_02445 0.71 +0.6 502,429 + mpl MCAODC_02445 0.72 +1.5 502,430 - mpl MCAODC_02445 0.72 -0.1 502,553 + mpl MCAODC_02445 0.81 -0.1 502,553 + mpl MCAODC_02445 0.81 +0.1 502,553 + mpl MCAODC_02445 0.81 +1.4 502,553 + mpl MCAODC_02445 0.81 +0.2 502,553 + mpl MCAODC_02445 0.81 -4.2 502,553 + mpl MCAODC_02445 0.81 -0.3 502,553 + mpl MCAODC_02445 0.81 +0.7 502,554 - mpl MCAODC_02445 0.81 +0.6 502,554 - mpl MCAODC_02445 0.81 -0.3 502,555 + mpl MCAODC_02445 0.81 -2.4 502,555 + mpl MCAODC_02445 0.81 +2.4 502,555 + mpl MCAODC_02445 0.81 +0.8 502,555 + mpl MCAODC_02445 0.81 +0.5 502,555 + mpl MCAODC_02445 0.81 -4.1 502,555 + mpl MCAODC_02445 0.81 +2.5 502,555 + mpl MCAODC_02445 0.81 +1.0 502,555 + mpl MCAODC_02445 0.81 -1.2 502,555 + mpl MCAODC_02445 0.81 +0.8 502,555 + mpl MCAODC_02445 0.81 -1.1 502,556 - mpl MCAODC_02445 0.81 +0.3 502,556 - mpl MCAODC_02445 0.81 +1.1 502,556 - mpl MCAODC_02445 0.81 +0.8 502,556 - mpl MCAODC_02445 0.81 -1.0 502,556 - mpl MCAODC_02445 0.81 +0.8 502,556 - mpl MCAODC_02445 0.81 -0.9 502,619 + mpl MCAODC_02445 0.86 +0.0 502,661 - mpl MCAODC_02445 0.89 -3.9 502,661 - mpl MCAODC_02445 0.89 -2.4 502,661 - mpl MCAODC_02445 0.89 +0.3 502,696 - -0.2 502,696 - -1.7 502,696 - +0.0 502,696 - -0.1 502,696 - +0.6 502,714 + +0.3 502,738 - -2.0 502,750 - -2.4 502,750 - -1.0 502,750 - -0.6 502,800 + +0.8 502,835 + +1.5 502,852 + -4.2 502,852 + -3.7 502,858 + +0.3 502,858 + +0.7 502,858 + -3.0 502,858 + -3.0 502,859 - +3.9 502,859 - -3.8 502,859 - +0.8 502,859 - +0.4 502,859 - +0.1 502,897 + +1.8 502,937 + -0.7 502,993 - -4.6 503,003 + -4.1 503,004 - -2.7 503,004 - -4.0 503,013 + -3.2 503,014 - -2.9 503,078 + -2.7 503,079 - -3.3 503,098 - fbp MCAODC_02450 0.11 -0.4 503,235 + fbp MCAODC_02450 0.24 -4.7 503,305 + fbp MCAODC_02450 0.31 -3.4 503,305 + fbp MCAODC_02450 0.31 -2.6 503,306 - fbp MCAODC_02450 0.31 -1.1 503,306 - fbp MCAODC_02450 0.31 -2.5 503,383 + fbp MCAODC_02450 0.39 -4.1 503,384 - fbp MCAODC_02450 0.39 -3.9 503,394 + fbp MCAODC_02450 0.40 -4.1 503,394 + fbp MCAODC_02450 0.40 -2.2 503,395 - fbp MCAODC_02450 0.40 -2.5 503,395 - fbp MCAODC_02450 0.40 -2.9 503,473 + fbp MCAODC_02450 0.48 -2.6 503,473 + fbp MCAODC_02450 0.48 -3.5 503,473 + fbp MCAODC_02450 0.48 -1.2 503,473 + fbp MCAODC_02450 0.48 -3.4 503,497 + fbp MCAODC_02450 0.50 -3.2 503,497 + fbp MCAODC_02450 0.50 -1.7 503,497 + fbp MCAODC_02450 0.50 +0.4 503,526 + fbp MCAODC_02450 0.53 -0.2 503,526 + fbp MCAODC_02450 0.53 -2.4 503,530 + fbp MCAODC_02450 0.54 -4.5 503,530 + fbp MCAODC_02450 0.54 -3.7 503,530 + fbp MCAODC_02450 0.54 -3.3 503,599 + fbp MCAODC_02450 0.61 -4.0 503,599 + fbp MCAODC_02450 0.61 -3.5 503,600 - fbp MCAODC_02450 0.61 -3.1 503,620 + fbp MCAODC_02450 0.63 -2.7 503,620 + fbp MCAODC_02450 0.63 -3.3 503,621 - fbp MCAODC_02450 0.63 -1.2 503,625 + fbp MCAODC_02450 0.63 -4.4 503,625 + fbp MCAODC_02450 0.63 -3.0 503,625 + fbp MCAODC_02450 0.63 -2.0 503,625 + fbp MCAODC_02450 0.63 -3.1 503,625 + fbp MCAODC_02450 0.63 -1.1 503,626 - fbp MCAODC_02450 0.63 -2.4 503,650 + fbp MCAODC_02450 0.66 -4.0 503,650 + fbp MCAODC_02450 0.66 -0.6 503,650 + fbp MCAODC_02450 0.66 -1.3 503,676 + fbp MCAODC_02450 0.68 -3.1 503,698 - fbp MCAODC_02450 0.71 -3.1 503,698 - fbp MCAODC_02450 0.71 -2.3 503,698 - fbp MCAODC_02450 0.71 -3.3 503,698 - fbp MCAODC_02450 0.71 -3.9 503,707 + fbp MCAODC_02450 0.71 -2.4 503,707 + fbp MCAODC_02450 0.71 -1.2 503,708 - fbp MCAODC_02450 0.72 -1.3 503,710 - fbp MCAODC_02450 0.72 -2.3 503,757 + fbp MCAODC_02450 0.76 -3.5 503,813 - fbp MCAODC_02450 0.82 -3.7
Or see this region's nucleotide sequence