Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt argF and MCAODC_02345 are separated by 56 nucleotides MCAODC_02345 and tabA are separated by 122 nucleotides tabA and bdcR are separated by 144 nucleotides
MCAODC_02340: argF - ornithine carbamoyltransferase, at 480,310 to 481,314
argF
MCAODC_02345: MCAODC_02345 - Uncharacterized protein YjgL, at 481,371 to 483,107
_02345
MCAODC_02350: tabA - toxin-antitoxin biofilm protein TabA, at 483,230 to 483,682
tabA
MCAODC_02355: bdcR - HTH-type transcriptional repressor BdcR, at 483,827 to 484,420
bdcR
Position (kb) 481
482
483
484 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 480.408 kb on + strand at 480.444 kb on + strand, within argF at 480.445 kb on - strand, within argF at 480.511 kb on + strand, within argF at 480.511 kb on + strand, within argF at 480.512 kb on - strand, within argF at 480.531 kb on + strand, within argF at 480.535 kb on + strand, within argF at 480.535 kb on + strand, within argF at 480.535 kb on + strand, within argF at 480.536 kb on - strand, within argF at 480.536 kb on - strand, within argF at 480.536 kb on - strand, within argF at 480.567 kb on + strand, within argF at 480.567 kb on + strand, within argF at 480.610 kb on + strand, within argF at 480.610 kb on + strand, within argF at 480.610 kb on + strand, within argF at 480.610 kb on + strand, within argF at 480.611 kb on - strand, within argF at 480.611 kb on - strand, within argF at 480.611 kb on - strand, within argF at 480.667 kb on + strand, within argF at 480.668 kb on - strand, within argF at 480.668 kb on - strand, within argF at 480.791 kb on - strand, within argF at 480.791 kb on - strand, within argF at 480.791 kb on - strand, within argF at 480.808 kb on - strand, within argF at 480.808 kb on - strand, within argF at 480.808 kb on - strand, within argF at 480.948 kb on + strand, within argF at 480.965 kb on + strand, within argF at 480.966 kb on - strand, within argF at 480.966 kb on - strand, within argF at 480.966 kb on - strand, within argF at 480.993 kb on + strand, within argF at 480.993 kb on + strand, within argF at 480.993 kb on + strand, within argF at 480.994 kb on - strand, within argF at 480.994 kb on - strand, within argF at 480.998 kb on - strand, within argF at 480.998 kb on - strand, within argF at 481.000 kb on - strand, within argF at 481.007 kb on + strand, within argF at 481.007 kb on + strand, within argF at 481.007 kb on + strand, within argF at 481.007 kb on + strand, within argF at 481.008 kb on - strand, within argF at 481.008 kb on - strand, within argF at 481.008 kb on - strand, within argF at 481.059 kb on - strand, within argF at 481.059 kb on - strand, within argF at 481.193 kb on + strand, within argF at 481.193 kb on + strand, within argF at 481.193 kb on + strand, within argF at 481.193 kb on + strand, within argF at 481.194 kb on - strand, within argF at 481.194 kb on - strand, within argF at 481.194 kb on - strand, within argF at 481.194 kb on - strand, within argF at 481.308 kb on + strand at 481.417 kb on - strand at 481.419 kb on - strand at 481.419 kb on - strand at 481.441 kb on - strand at 481.655 kb on + strand, within MCAODC_02345 at 481.776 kb on - strand, within MCAODC_02345 at 481.836 kb on + strand, within MCAODC_02345 at 482.139 kb on - strand, within MCAODC_02345 at 482.225 kb on + strand, within MCAODC_02345 at 482.259 kb on + strand, within MCAODC_02345 at 482.279 kb on + strand, within MCAODC_02345 at 482.279 kb on + strand, within MCAODC_02345 at 482.317 kb on - strand, within MCAODC_02345 at 482.325 kb on + strand, within MCAODC_02345 at 482.460 kb on + strand, within MCAODC_02345 at 482.547 kb on - strand, within MCAODC_02345 at 482.729 kb on + strand, within MCAODC_02345 at 483.137 kb on + strand at 483.212 kb on - strand at 483.298 kb on + strand, within tabA at 483.347 kb on - strand, within tabA at 483.399 kb on - strand, within tabA at 483.484 kb on - strand, within tabA at 483.495 kb on + strand, within tabA at 483.529 kb on + strand, within tabA at 483.546 kb on + strand, within tabA at 483.546 kb on + strand, within tabA at 483.613 kb on + strand, within tabA at 483.613 kb on + strand, within tabA at 483.613 kb on + strand, within tabA at 483.614 kb on - strand, within tabA at 483.614 kb on - strand, within tabA at 483.633 kb on - strand, within tabA at 483.667 kb on + strand at 483.668 kb on - strand at 483.769 kb on - strand at 483.776 kb on + strand at 483.841 kb on + strand at 483.849 kb on - strand at 483.937 kb on + strand, within bdcR at 483.943 kb on + strand, within bdcR at 483.944 kb on - strand, within bdcR at 483.988 kb on + strand, within bdcR at 483.990 kb on + strand, within bdcR at 483.990 kb on + strand, within bdcR at 483.990 kb on + strand, within bdcR at 483.990 kb on + strand, within bdcR at 483.990 kb on + strand, within bdcR at 483.990 kb on + strand, within bdcR at 483.991 kb on - strand, within bdcR at 483.996 kb on + strand, within bdcR at 483.996 kb on + strand, within bdcR at 483.996 kb on + strand, within bdcR at 483.996 kb on + strand, within bdcR at 483.996 kb on + strand, within bdcR at 483.997 kb on - strand, within bdcR at 484.021 kb on - strand, within bdcR at 484.021 kb on - strand, within bdcR at 484.021 kb on - strand, within bdcR at 484.023 kb on + strand, within bdcR at 484.037 kb on - strand, within bdcR at 484.061 kb on - strand, within bdcR at 484.061 kb on - strand, within bdcR at 484.061 kb on - strand, within bdcR at 484.061 kb on - strand, within bdcR at 484.082 kb on - strand, within bdcR
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 480,408 + +1.1 480,444 + argF MCAODC_02340 0.13 +0.2 480,445 - argF MCAODC_02340 0.13 +1.0 480,511 + argF MCAODC_02340 0.20 +0.1 480,511 + argF MCAODC_02340 0.20 +1.3 480,512 - argF MCAODC_02340 0.20 +1.4 480,531 + argF MCAODC_02340 0.22 +1.5 480,535 + argF MCAODC_02340 0.22 -0.9 480,535 + argF MCAODC_02340 0.22 -0.3 480,535 + argF MCAODC_02340 0.22 -1.7 480,536 - argF MCAODC_02340 0.22 +1.0 480,536 - argF MCAODC_02340 0.22 +1.5 480,536 - argF MCAODC_02340 0.22 -1.3 480,567 + argF MCAODC_02340 0.26 +0.3 480,567 + argF MCAODC_02340 0.26 +0.6 480,610 + argF MCAODC_02340 0.30 +0.2 480,610 + argF MCAODC_02340 0.30 +0.3 480,610 + argF MCAODC_02340 0.30 +0.4 480,610 + argF MCAODC_02340 0.30 -0.7 480,611 - argF MCAODC_02340 0.30 +0.4 480,611 - argF MCAODC_02340 0.30 +0.8 480,611 - argF MCAODC_02340 0.30 +0.1 480,667 + argF MCAODC_02340 0.36 -0.1 480,668 - argF MCAODC_02340 0.36 -0.8 480,668 - argF MCAODC_02340 0.36 +0.3 480,791 - argF MCAODC_02340 0.48 +0.6 480,791 - argF MCAODC_02340 0.48 +2.5 480,791 - argF MCAODC_02340 0.48 -0.2 480,808 - argF MCAODC_02340 0.50 -2.1 480,808 - argF MCAODC_02340 0.50 -2.4 480,808 - argF MCAODC_02340 0.50 -0.2 480,948 + argF MCAODC_02340 0.63 -1.0 480,965 + argF MCAODC_02340 0.65 +0.7 480,966 - argF MCAODC_02340 0.65 +1.7 480,966 - argF MCAODC_02340 0.65 -0.2 480,966 - argF MCAODC_02340 0.65 +2.2 480,993 + argF MCAODC_02340 0.68 +0.2 480,993 + argF MCAODC_02340 0.68 -3.3 480,993 + argF MCAODC_02340 0.68 +1.0 480,994 - argF MCAODC_02340 0.68 +0.1 480,994 - argF MCAODC_02340 0.68 +1.6 480,998 - argF MCAODC_02340 0.68 -3.5 480,998 - argF MCAODC_02340 0.68 +1.2 481,000 - argF MCAODC_02340 0.69 -0.9 481,007 + argF MCAODC_02340 0.69 -0.4 481,007 + argF MCAODC_02340 0.69 +0.7 481,007 + argF MCAODC_02340 0.69 -3.2 481,007 + argF MCAODC_02340 0.69 +0.4 481,008 - argF MCAODC_02340 0.69 -2.1 481,008 - argF MCAODC_02340 0.69 -2.0 481,008 - argF MCAODC_02340 0.69 +0.3 481,059 - argF MCAODC_02340 0.75 -1.8 481,059 - argF MCAODC_02340 0.75 -3.9 481,193 + argF MCAODC_02340 0.88 +2.7 481,193 + argF MCAODC_02340 0.88 +0.3 481,193 + argF MCAODC_02340 0.88 +1.7 481,193 + argF MCAODC_02340 0.88 +1.5 481,194 - argF MCAODC_02340 0.88 +0.2 481,194 - argF MCAODC_02340 0.88 +1.3 481,194 - argF MCAODC_02340 0.88 +0.4 481,194 - argF MCAODC_02340 0.88 -2.8 481,308 + +0.5 481,417 - +0.5 481,419 - +0.6 481,419 - -0.0 481,441 - -2.8 481,655 + MCAODC_02345 0.16 +2.3 481,776 - MCAODC_02345 0.23 -2.4 481,836 + MCAODC_02345 0.27 -0.6 482,139 - MCAODC_02345 0.44 +2.4 482,225 + MCAODC_02345 0.49 -1.1 482,259 + MCAODC_02345 0.51 +0.6 482,279 + MCAODC_02345 0.52 +1.8 482,279 + MCAODC_02345 0.52 +1.0 482,317 - MCAODC_02345 0.54 +1.4 482,325 + MCAODC_02345 0.55 +0.9 482,460 + MCAODC_02345 0.63 -0.9 482,547 - MCAODC_02345 0.68 +1.2 482,729 + MCAODC_02345 0.78 +0.1 483,137 + +0.2 483,212 - -3.0 483,298 + tabA MCAODC_02350 0.15 -3.8 483,347 - tabA MCAODC_02350 0.26 +0.5 483,399 - tabA MCAODC_02350 0.37 -2.1 483,484 - tabA MCAODC_02350 0.56 +0.7 483,495 + tabA MCAODC_02350 0.58 -3.1 483,529 + tabA MCAODC_02350 0.66 +0.6 483,546 + tabA MCAODC_02350 0.70 -0.5 483,546 + tabA MCAODC_02350 0.70 +0.5 483,613 + tabA MCAODC_02350 0.85 +0.7 483,613 + tabA MCAODC_02350 0.85 +0.7 483,613 + tabA MCAODC_02350 0.85 +0.5 483,614 - tabA MCAODC_02350 0.85 -0.2 483,614 - tabA MCAODC_02350 0.85 +0.3 483,633 - tabA MCAODC_02350 0.89 -0.2 483,667 + -0.5 483,668 - +0.6 483,769 - +0.2 483,776 + -2.2 483,841 + +1.7 483,849 - +0.7 483,937 + bdcR MCAODC_02355 0.19 +0.6 483,943 + bdcR MCAODC_02355 0.20 -1.7 483,944 - bdcR MCAODC_02355 0.20 -0.9 483,988 + bdcR MCAODC_02355 0.27 +1.2 483,990 + bdcR MCAODC_02355 0.27 -1.2 483,990 + bdcR MCAODC_02355 0.27 -3.9 483,990 + bdcR MCAODC_02355 0.27 -0.4 483,990 + bdcR MCAODC_02355 0.27 +2.2 483,990 + bdcR MCAODC_02355 0.27 +1.9 483,990 + bdcR MCAODC_02355 0.27 +0.6 483,991 - bdcR MCAODC_02355 0.28 +2.3 483,996 + bdcR MCAODC_02355 0.28 +1.4 483,996 + bdcR MCAODC_02355 0.28 +1.1 483,996 + bdcR MCAODC_02355 0.28 +1.2 483,996 + bdcR MCAODC_02355 0.28 +0.6 483,996 + bdcR MCAODC_02355 0.28 +0.6 483,997 - bdcR MCAODC_02355 0.29 +0.7 484,021 - bdcR MCAODC_02355 0.33 +0.9 484,021 - bdcR MCAODC_02355 0.33 -0.2 484,021 - bdcR MCAODC_02355 0.33 +1.6 484,023 + bdcR MCAODC_02355 0.33 +0.4 484,037 - bdcR MCAODC_02355 0.35 +0.2 484,061 - bdcR MCAODC_02355 0.39 +0.2 484,061 - bdcR MCAODC_02355 0.39 +0.4 484,061 - bdcR MCAODC_02355 0.39 +3.3 484,061 - bdcR MCAODC_02355 0.39 -0.4 484,082 - bdcR MCAODC_02355 0.43 -0.6
Or see this region's nucleotide sequence