Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt iadA and hypT are separated by 247 nucleotides hypT and iraD overlap by 8 nucleotides iraD and idlP overlap by 4 nucleotides idlP and MCAODC_02060 are separated by 237 nucleotides
MCAODC_02040: iadA - beta-aspartyl-peptidase, at 401,269 to 402,441
iadA
MCAODC_02045: hypT - hypochlorite stress DNA-binding transcriptional regulator HypT, at 402,689 to 403,417
hypT
MCAODC_02050: iraD - anti-adapter protein IraD, at 403,410 to 403,802
iraD
MCAODC_02055: idlP - iraD leader peptide, at 403,799 to 403,882
idlP
MCAODC_02060: MCAODC_02060 - hypothetical protein, at 404,120 to 404,221
_02060
Position (kb) 402
403
404 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 401.794 kb on + strand, within iadA at 401.795 kb on - strand, within iadA at 401.795 kb on - strand, within iadA at 401.884 kb on - strand, within iadA at 401.884 kb on - strand, within iadA at 401.894 kb on + strand, within iadA at 401.894 kb on + strand, within iadA at 401.894 kb on + strand, within iadA at 401.902 kb on - strand, within iadA at 401.902 kb on - strand, within iadA at 401.902 kb on - strand, within iadA at 401.902 kb on - strand, within iadA at 401.902 kb on - strand, within iadA at 401.906 kb on - strand, within iadA at 401.906 kb on - strand, within iadA at 401.906 kb on - strand, within iadA at 401.906 kb on - strand, within iadA at 401.906 kb on - strand, within iadA at 401.972 kb on + strand, within iadA at 401.972 kb on + strand, within iadA at 401.973 kb on - strand, within iadA at 401.973 kb on - strand, within iadA at 401.973 kb on - strand, within iadA at 401.973 kb on - strand, within iadA at 402.229 kb on + strand, within iadA at 402.336 kb on - strand at 402.336 kb on - strand at 402.371 kb on + strand at 402.373 kb on + strand at 402.373 kb on + strand at 402.373 kb on + strand at 402.373 kb on + strand at 402.373 kb on + strand at 402.374 kb on - strand at 402.374 kb on - strand at 402.440 kb on - strand at 402.460 kb on - strand at 402.497 kb on + strand at 402.504 kb on + strand at 402.504 kb on + strand at 402.505 kb on - strand at 402.532 kb on + strand at 402.760 kb on - strand at 402.760 kb on - strand at 402.760 kb on - strand at 402.808 kb on + strand, within hypT at 402.808 kb on + strand, within hypT at 402.809 kb on - strand, within hypT at 402.886 kb on + strand, within hypT at 402.886 kb on + strand, within hypT at 403.012 kb on - strand, within hypT at 403.012 kb on - strand, within hypT at 403.104 kb on - strand, within hypT at 403.119 kb on - strand, within hypT at 403.136 kb on - strand, within hypT at 403.136 kb on - strand, within hypT at 403.207 kb on - strand, within hypT at 403.253 kb on + strand, within hypT at 403.253 kb on + strand, within hypT at 403.254 kb on - strand, within hypT at 403.412 kb on - strand at 403.500 kb on + strand, within iraD at 403.500 kb on + strand, within iraD at 403.501 kb on - strand, within iraD at 403.581 kb on + strand, within iraD at 403.581 kb on + strand, within iraD at 403.582 kb on - strand, within iraD at 403.610 kb on - strand, within iraD at 403.674 kb on + strand, within iraD at 403.674 kb on + strand, within iraD at 403.674 kb on + strand, within iraD at 403.674 kb on + strand, within iraD at 403.674 kb on + strand, within iraD at 403.675 kb on - strand, within iraD at 403.684 kb on - strand, within iraD at 403.703 kb on + strand, within iraD at 403.822 kb on + strand, within idlP at 403.823 kb on - strand, within idlP at 403.830 kb on + strand, within idlP at 403.830 kb on + strand, within idlP at 403.831 kb on - strand, within idlP at 403.831 kb on - strand, within idlP at 403.864 kb on - strand, within idlP at 403.864 kb on - strand, within idlP at 403.903 kb on + strand at 403.904 kb on - strand at 403.904 kb on - strand at 403.904 kb on - strand at 403.904 kb on - strand at 404.044 kb on + strand at 404.066 kb on + strand at 404.067 kb on - strand at 404.067 kb on - strand at 404.070 kb on + strand at 404.071 kb on - strand at 404.071 kb on - strand at 404.225 kb on + strand at 404.326 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 401,794 + iadA MCAODC_02040 0.45 +2.7 401,795 - iadA MCAODC_02040 0.45 +0.3 401,795 - iadA MCAODC_02040 0.45 +0.5 401,884 - iadA MCAODC_02040 0.52 +1.0 401,884 - iadA MCAODC_02040 0.52 +1.3 401,894 + iadA MCAODC_02040 0.53 -0.6 401,894 + iadA MCAODC_02040 0.53 +0.9 401,894 + iadA MCAODC_02040 0.53 +0.5 401,902 - iadA MCAODC_02040 0.54 -0.0 401,902 - iadA MCAODC_02040 0.54 +0.9 401,902 - iadA MCAODC_02040 0.54 -0.3 401,902 - iadA MCAODC_02040 0.54 -1.1 401,902 - iadA MCAODC_02040 0.54 +0.5 401,906 - iadA MCAODC_02040 0.54 +0.2 401,906 - iadA MCAODC_02040 0.54 -1.4 401,906 - iadA MCAODC_02040 0.54 +0.7 401,906 - iadA MCAODC_02040 0.54 -1.1 401,906 - iadA MCAODC_02040 0.54 -0.8 401,972 + iadA MCAODC_02040 0.60 +0.7 401,972 + iadA MCAODC_02040 0.60 +0.9 401,973 - iadA MCAODC_02040 0.60 +1.0 401,973 - iadA MCAODC_02040 0.60 -1.3 401,973 - iadA MCAODC_02040 0.60 +0.1 401,973 - iadA MCAODC_02040 0.60 -1.9 402,229 + iadA MCAODC_02040 0.82 -2.1 402,336 - -0.9 402,336 - -0.2 402,371 + +0.7 402,373 + +0.9 402,373 + -1.5 402,373 + +0.5 402,373 + +2.3 402,373 + +1.6 402,374 - -2.3 402,374 - -0.8 402,440 - -0.5 402,460 - +0.1 402,497 + -1.5 402,504 + -1.1 402,504 + +1.1 402,505 - +0.7 402,532 + -0.8 402,760 - -0.6 402,760 - -3.3 402,760 - +0.2 402,808 + hypT MCAODC_02045 0.16 -0.1 402,808 + hypT MCAODC_02045 0.16 -2.0 402,809 - hypT MCAODC_02045 0.16 +0.0 402,886 + hypT MCAODC_02045 0.27 -0.6 402,886 + hypT MCAODC_02045 0.27 +0.3 403,012 - hypT MCAODC_02045 0.44 -0.8 403,012 - hypT MCAODC_02045 0.44 -0.6 403,104 - hypT MCAODC_02045 0.57 -2.4 403,119 - hypT MCAODC_02045 0.59 -1.4 403,136 - hypT MCAODC_02045 0.61 +0.3 403,136 - hypT MCAODC_02045 0.61 +2.2 403,207 - hypT MCAODC_02045 0.71 -0.3 403,253 + hypT MCAODC_02045 0.77 -3.4 403,253 + hypT MCAODC_02045 0.77 -1.7 403,254 - hypT MCAODC_02045 0.78 -1.3 403,412 - -4.1 403,500 + iraD MCAODC_02050 0.23 +0.3 403,500 + iraD MCAODC_02050 0.23 -3.8 403,501 - iraD MCAODC_02050 0.23 +0.0 403,581 + iraD MCAODC_02050 0.44 +2.0 403,581 + iraD MCAODC_02050 0.44 +1.1 403,582 - iraD MCAODC_02050 0.44 +0.9 403,610 - iraD MCAODC_02050 0.51 +0.1 403,674 + iraD MCAODC_02050 0.67 +1.0 403,674 + iraD MCAODC_02050 0.67 -0.3 403,674 + iraD MCAODC_02050 0.67 +2.4 403,674 + iraD MCAODC_02050 0.67 -1.1 403,674 + iraD MCAODC_02050 0.67 +0.6 403,675 - iraD MCAODC_02050 0.67 +0.1 403,684 - iraD MCAODC_02050 0.70 +0.9 403,703 + iraD MCAODC_02050 0.75 +1.0 403,822 + idlP MCAODC_02055 0.27 -0.7 403,823 - idlP MCAODC_02055 0.29 +1.3 403,830 + idlP MCAODC_02055 0.37 -2.5 403,830 + idlP MCAODC_02055 0.37 +0.5 403,831 - idlP MCAODC_02055 0.38 -1.1 403,831 - idlP MCAODC_02055 0.38 -2.2 403,864 - idlP MCAODC_02055 0.77 +1.7 403,864 - idlP MCAODC_02055 0.77 +0.2 403,903 + +1.2 403,904 - +0.2 403,904 - -0.1 403,904 - -3.4 403,904 - -0.9 404,044 + -1.3 404,066 + +0.7 404,067 - -1.9 404,067 - -0.0 404,070 + +0.1 404,071 - -2.6 404,071 - -0.1 404,225 + -0.2 404,326 - -2.2
Or see this region's nucleotide sequence