Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt deoC and yjjI are separated by 257 nucleotides yjjI and MCAODC_01755 are separated by 35 nucleotides MCAODC_01755 and yjjW overlap by 29 nucleotides
MCAODC_01745: deoC - deoxyribose-phosphate aldolase, at 339,697 to 340,476
deoC
MCAODC_01750: yjjI - Glycine radical enzyme, YjjI family, at 340,734 to 342,002
yjjI
MCAODC_01755: MCAODC_01755 - Glycine radical enzyme, YjjI family, at 342,038 to 342,283
_01755
MCAODC_01760: yjjW - Putative glycyl-radical enzyme activating enzyme YjjW, at 342,255 to 343,118
yjjW
Position (kb) 340
341
342
343 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 339.780 kb on - strand, within deoC at 339.833 kb on + strand, within deoC at 339.834 kb on - strand, within deoC at 339.896 kb on - strand, within deoC at 339.904 kb on - strand, within deoC at 339.904 kb on - strand, within deoC at 340.076 kb on - strand, within deoC at 340.076 kb on - strand, within deoC at 340.102 kb on - strand, within deoC at 340.102 kb on - strand, within deoC at 340.332 kb on - strand, within deoC at 340.335 kb on + strand, within deoC at 340.335 kb on + strand, within deoC at 340.335 kb on + strand, within deoC at 340.335 kb on + strand, within deoC at 340.336 kb on - strand, within deoC at 340.336 kb on - strand, within deoC at 340.342 kb on - strand, within deoC at 340.342 kb on - strand, within deoC at 340.342 kb on - strand, within deoC at 340.532 kb on + strand at 340.540 kb on - strand at 340.559 kb on + strand at 340.559 kb on + strand at 340.560 kb on - strand at 340.560 kb on - strand at 340.561 kb on + strand at 340.561 kb on + strand at 340.561 kb on + strand at 340.561 kb on + strand at 340.562 kb on - strand at 340.562 kb on - strand at 340.562 kb on - strand at 340.562 kb on - strand at 340.562 kb on - strand at 340.562 kb on - strand at 340.582 kb on + strand at 340.583 kb on - strand at 340.583 kb on - strand at 340.587 kb on - strand at 340.602 kb on + strand at 340.602 kb on + strand at 340.602 kb on + strand at 340.603 kb on - strand at 340.603 kb on - strand at 340.603 kb on - strand at 340.603 kb on - strand at 340.603 kb on - strand at 340.603 kb on - strand at 340.604 kb on + strand at 340.605 kb on - strand at 340.624 kb on - strand at 340.695 kb on + strand at 340.695 kb on + strand at 340.696 kb on - strand at 340.696 kb on - strand at 340.719 kb on + strand at 340.799 kb on + strand at 340.800 kb on - strand at 340.900 kb on + strand, within yjjI at 340.901 kb on - strand, within yjjI at 340.901 kb on - strand, within yjjI at 340.901 kb on - strand, within yjjI at 340.901 kb on - strand, within yjjI at 340.910 kb on + strand, within yjjI at 340.944 kb on + strand, within yjjI at 340.944 kb on + strand, within yjjI at 341.049 kb on + strand, within yjjI at 341.049 kb on + strand, within yjjI at 341.059 kb on + strand, within yjjI at 341.060 kb on - strand, within yjjI at 341.086 kb on - strand, within yjjI at 341.086 kb on - strand, within yjjI at 341.086 kb on - strand, within yjjI at 341.443 kb on - strand, within yjjI at 341.548 kb on + strand, within yjjI at 341.549 kb on - strand, within yjjI at 341.556 kb on + strand, within yjjI at 341.556 kb on + strand, within yjjI at 341.556 kb on + strand, within yjjI at 341.557 kb on - strand, within yjjI at 341.604 kb on + strand, within yjjI at 341.604 kb on + strand, within yjjI at 341.630 kb on + strand, within yjjI at 341.631 kb on - strand, within yjjI at 341.691 kb on + strand, within yjjI at 341.691 kb on + strand, within yjjI at 341.692 kb on - strand, within yjjI at 341.692 kb on - strand, within yjjI at 341.692 kb on - strand, within yjjI at 341.692 kb on - strand, within yjjI at 341.776 kb on + strand, within yjjI at 341.776 kb on + strand, within yjjI at 341.777 kb on - strand, within yjjI at 341.777 kb on - strand, within yjjI at 341.777 kb on - strand, within yjjI at 341.777 kb on - strand, within yjjI at 341.777 kb on - strand, within yjjI at 341.777 kb on - strand, within yjjI at 341.832 kb on + strand, within yjjI at 341.832 kb on + strand, within yjjI at 341.832 kb on + strand, within yjjI at 341.832 kb on + strand, within yjjI at 341.832 kb on + strand, within yjjI at 341.857 kb on + strand, within yjjI at 341.857 kb on + strand, within yjjI at 341.972 kb on + strand at 341.972 kb on + strand at 341.972 kb on + strand at 341.972 kb on + strand at 341.973 kb on - strand at 341.973 kb on - strand at 341.975 kb on + strand at 341.975 kb on + strand at 341.975 kb on + strand at 341.976 kb on - strand at 341.976 kb on - strand at 341.978 kb on + strand at 342.026 kb on - strand at 342.043 kb on - strand at 342.085 kb on + strand, within MCAODC_01755 at 342.128 kb on + strand, within MCAODC_01755 at 342.129 kb on - strand, within MCAODC_01755 at 342.129 kb on - strand, within MCAODC_01755 at 342.130 kb on + strand, within MCAODC_01755 at 342.130 kb on + strand, within MCAODC_01755 at 342.130 kb on + strand, within MCAODC_01755 at 342.130 kb on + strand, within MCAODC_01755 at 342.130 kb on + strand, within MCAODC_01755 at 342.130 kb on + strand, within MCAODC_01755 at 342.130 kb on + strand, within MCAODC_01755 at 342.130 kb on + strand, within MCAODC_01755 at 342.131 kb on - strand, within MCAODC_01755 at 342.131 kb on - strand, within MCAODC_01755 at 342.131 kb on - strand, within MCAODC_01755 at 342.131 kb on - strand, within MCAODC_01755 at 342.131 kb on - strand, within MCAODC_01755 at 342.249 kb on + strand, within MCAODC_01755 at 342.275 kb on - strand at 342.281 kb on + strand at 342.494 kb on + strand, within yjjW at 342.495 kb on - strand, within yjjW at 342.495 kb on - strand, within yjjW at 342.644 kb on - strand, within yjjW at 342.656 kb on + strand, within yjjW at 342.663 kb on - strand, within yjjW at 342.710 kb on + strand, within yjjW at 342.710 kb on + strand, within yjjW at 342.716 kb on + strand, within yjjW at 342.716 kb on + strand, within yjjW at 342.717 kb on - strand, within yjjW at 342.717 kb on - strand, within yjjW at 342.825 kb on - strand, within yjjW at 342.836 kb on + strand, within yjjW at 342.836 kb on + strand, within yjjW at 342.837 kb on - strand, within yjjW at 342.870 kb on - strand, within yjjW
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 339,780 - deoC MCAODC_01745 0.11 -3.0 339,833 + deoC MCAODC_01745 0.17 -1.7 339,834 - deoC MCAODC_01745 0.18 -2.2 339,896 - deoC MCAODC_01745 0.26 +0.4 339,904 - deoC MCAODC_01745 0.27 -0.0 339,904 - deoC MCAODC_01745 0.27 -1.1 340,076 - deoC MCAODC_01745 0.49 +0.6 340,076 - deoC MCAODC_01745 0.49 +2.1 340,102 - deoC MCAODC_01745 0.52 +0.0 340,102 - deoC MCAODC_01745 0.52 +0.3 340,332 - deoC MCAODC_01745 0.81 +0.8 340,335 + deoC MCAODC_01745 0.82 -0.8 340,335 + deoC MCAODC_01745 0.82 +0.3 340,335 + deoC MCAODC_01745 0.82 -0.2 340,335 + deoC MCAODC_01745 0.82 -2.7 340,336 - deoC MCAODC_01745 0.82 -2.9 340,336 - deoC MCAODC_01745 0.82 -3.2 340,342 - deoC MCAODC_01745 0.83 +1.2 340,342 - deoC MCAODC_01745 0.83 +2.2 340,342 - deoC MCAODC_01745 0.83 +1.5 340,532 + -0.4 340,540 - +0.6 340,559 + +0.6 340,559 + +1.2 340,560 - +0.1 340,560 - +0.7 340,561 + -0.3 340,561 + +0.7 340,561 + -0.6 340,561 + -2.7 340,562 - +0.3 340,562 - +1.7 340,562 - -2.0 340,562 - +0.2 340,562 - -2.4 340,562 - +0.1 340,582 + -2.4 340,583 - -2.8 340,583 - -0.3 340,587 - -0.6 340,602 + +0.4 340,602 + +0.0 340,602 + +1.0 340,603 - -2.0 340,603 - -0.0 340,603 - -3.3 340,603 - -1.2 340,603 - -0.6 340,603 - -0.3 340,604 + -0.0 340,605 - +0.9 340,624 - +0.9 340,695 + -1.3 340,695 + -0.7 340,696 - +0.7 340,696 - -1.0 340,719 + -3.2 340,799 + -1.4 340,800 - -0.1 340,900 + yjjI MCAODC_01750 0.13 -3.7 340,901 - yjjI MCAODC_01750 0.13 +0.0 340,901 - yjjI MCAODC_01750 0.13 +0.6 340,901 - yjjI MCAODC_01750 0.13 +1.2 340,901 - yjjI MCAODC_01750 0.13 -1.2 340,910 + yjjI MCAODC_01750 0.14 -3.0 340,944 + yjjI MCAODC_01750 0.17 -0.6 340,944 + yjjI MCAODC_01750 0.17 +2.9 341,049 + yjjI MCAODC_01750 0.25 -1.4 341,049 + yjjI MCAODC_01750 0.25 +1.7 341,059 + yjjI MCAODC_01750 0.26 -0.2 341,060 - yjjI MCAODC_01750 0.26 +1.4 341,086 - yjjI MCAODC_01750 0.28 +0.5 341,086 - yjjI MCAODC_01750 0.28 +0.3 341,086 - yjjI MCAODC_01750 0.28 +0.2 341,443 - yjjI MCAODC_01750 0.56 -0.3 341,548 + yjjI MCAODC_01750 0.64 +0.5 341,549 - yjjI MCAODC_01750 0.64 +1.0 341,556 + yjjI MCAODC_01750 0.65 +1.4 341,556 + yjjI MCAODC_01750 0.65 -3.2 341,556 + yjjI MCAODC_01750 0.65 +2.6 341,557 - yjjI MCAODC_01750 0.65 +1.8 341,604 + yjjI MCAODC_01750 0.69 +0.4 341,604 + yjjI MCAODC_01750 0.69 -1.4 341,630 + yjjI MCAODC_01750 0.71 -1.8 341,631 - yjjI MCAODC_01750 0.71 -2.6 341,691 + yjjI MCAODC_01750 0.75 -0.0 341,691 + yjjI MCAODC_01750 0.75 +0.3 341,692 - yjjI MCAODC_01750 0.75 -4.2 341,692 - yjjI MCAODC_01750 0.75 +0.4 341,692 - yjjI MCAODC_01750 0.75 +0.5 341,692 - yjjI MCAODC_01750 0.75 -0.3 341,776 + yjjI MCAODC_01750 0.82 -3.0 341,776 + yjjI MCAODC_01750 0.82 -1.7 341,777 - yjjI MCAODC_01750 0.82 -1.4 341,777 - yjjI MCAODC_01750 0.82 +0.5 341,777 - yjjI MCAODC_01750 0.82 -3.6 341,777 - yjjI MCAODC_01750 0.82 +1.2 341,777 - yjjI MCAODC_01750 0.82 -0.4 341,777 - yjjI MCAODC_01750 0.82 +1.4 341,832 + yjjI MCAODC_01750 0.87 +0.1 341,832 + yjjI MCAODC_01750 0.87 -3.5 341,832 + yjjI MCAODC_01750 0.87 -0.8 341,832 + yjjI MCAODC_01750 0.87 +1.5 341,832 + yjjI MCAODC_01750 0.87 -1.0 341,857 + yjjI MCAODC_01750 0.88 -2.0 341,857 + yjjI MCAODC_01750 0.88 +0.4 341,972 + +1.5 341,972 + -0.8 341,972 + -0.5 341,972 + +0.2 341,973 - +0.2 341,973 - +0.6 341,975 + +0.3 341,975 + +0.8 341,975 + -3.1 341,976 - -0.0 341,976 - -0.5 341,978 + +1.1 342,026 - +1.0 342,043 - +1.7 342,085 + MCAODC_01755 0.19 -0.4 342,128 + MCAODC_01755 0.37 +2.5 342,129 - MCAODC_01755 0.37 -1.8 342,129 - MCAODC_01755 0.37 +2.3 342,130 + MCAODC_01755 0.37 -0.1 342,130 + MCAODC_01755 0.37 +0.1 342,130 + MCAODC_01755 0.37 -1.2 342,130 + MCAODC_01755 0.37 -0.5 342,130 + MCAODC_01755 0.37 -0.4 342,130 + MCAODC_01755 0.37 +1.4 342,130 + MCAODC_01755 0.37 -2.2 342,130 + MCAODC_01755 0.37 -2.1 342,131 - MCAODC_01755 0.38 +0.1 342,131 - MCAODC_01755 0.38 +0.6 342,131 - MCAODC_01755 0.38 +2.4 342,131 - MCAODC_01755 0.38 -3.6 342,131 - MCAODC_01755 0.38 +1.8 342,249 + MCAODC_01755 0.86 +0.8 342,275 - +0.0 342,281 + +0.4 342,494 + yjjW MCAODC_01760 0.28 -3.5 342,495 - yjjW MCAODC_01760 0.28 -0.7 342,495 - yjjW MCAODC_01760 0.28 -0.5 342,644 - yjjW MCAODC_01760 0.45 +0.9 342,656 + yjjW MCAODC_01760 0.46 +1.2 342,663 - yjjW MCAODC_01760 0.47 +1.0 342,710 + yjjW MCAODC_01760 0.53 -0.3 342,710 + yjjW MCAODC_01760 0.53 -1.9 342,716 + yjjW MCAODC_01760 0.53 +0.6 342,716 + yjjW MCAODC_01760 0.53 +1.6 342,717 - yjjW MCAODC_01760 0.53 +0.6 342,717 - yjjW MCAODC_01760 0.53 +1.0 342,825 - yjjW MCAODC_01760 0.66 -0.2 342,836 + yjjW MCAODC_01760 0.67 +0.8 342,836 + yjjW MCAODC_01760 0.67 +1.7 342,837 - yjjW MCAODC_01760 0.67 +0.8 342,870 - yjjW MCAODC_01760 0.71 -4.3
Or see this region's nucleotide sequence