Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nhaR and nhaA are separated by 65 nucleotides nhaA and mokC are separated by 528 nucleotides mokC and dnaJ are separated by 103 nucleotides
MCAODC_01540: nhaR - transcriptional activator NhaR, at 298,902 to 299,801
nhaR
MCAODC_01545: nhaA - Na+/H+ antiporter NhaA, at 299,867 to 301,033
nhaA
MCAODC_01555: mokC - Regulatory protein MokC, at 301,562 to 301,771
mokC
MCAODC_01560: dnaJ - molecular chaperone DnaJ, at 301,875 to 303,005
dnaJ
Position (kb) 299
300
301
302 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 298.867 kb on + strand at 298.868 kb on - strand at 298.868 kb on - strand at 298.881 kb on + strand at 298.882 kb on - strand at 298.920 kb on - strand at 298.992 kb on - strand, within nhaR at 298.992 kb on - strand, within nhaR at 298.999 kb on + strand, within nhaR at 299.054 kb on - strand, within nhaR at 299.068 kb on + strand, within nhaR at 299.069 kb on - strand, within nhaR at 299.155 kb on - strand, within nhaR at 299.160 kb on + strand, within nhaR at 299.174 kb on + strand, within nhaR at 299.183 kb on + strand, within nhaR at 299.183 kb on + strand, within nhaR at 299.184 kb on - strand, within nhaR at 299.187 kb on + strand, within nhaR at 299.188 kb on - strand, within nhaR at 299.188 kb on - strand, within nhaR at 299.280 kb on - strand, within nhaR at 299.388 kb on + strand, within nhaR at 299.388 kb on + strand, within nhaR at 299.451 kb on + strand, within nhaR at 299.451 kb on + strand, within nhaR at 299.496 kb on + strand, within nhaR at 299.497 kb on - strand, within nhaR at 299.542 kb on + strand, within nhaR at 299.576 kb on + strand, within nhaR at 299.599 kb on + strand, within nhaR at 299.649 kb on - strand, within nhaR at 299.649 kb on - strand, within nhaR at 299.652 kb on + strand, within nhaR at 299.652 kb on + strand, within nhaR at 299.653 kb on - strand, within nhaR at 299.712 kb on + strand at 299.716 kb on + strand at 299.716 kb on + strand at 299.717 kb on - strand at 299.753 kb on + strand at 299.753 kb on + strand at 299.754 kb on - strand at 299.755 kb on + strand at 299.755 kb on + strand at 299.756 kb on - strand at 299.770 kb on + strand at 299.773 kb on + strand at 299.773 kb on + strand at 299.773 kb on + strand at 299.773 kb on + strand at 299.773 kb on + strand at 299.774 kb on - strand at 299.774 kb on - strand at 299.774 kb on - strand at 299.786 kb on - strand at 299.927 kb on - strand at 299.927 kb on - strand at 299.975 kb on + strand at 299.976 kb on - strand at 299.981 kb on - strand at 300.001 kb on + strand, within nhaA at 300.007 kb on + strand, within nhaA at 300.007 kb on + strand, within nhaA at 300.007 kb on + strand, within nhaA at 300.013 kb on + strand, within nhaA at 300.013 kb on + strand, within nhaA at 300.013 kb on + strand, within nhaA at 300.013 kb on + strand, within nhaA at 300.014 kb on - strand, within nhaA at 300.014 kb on - strand, within nhaA at 300.016 kb on + strand, within nhaA at 300.017 kb on - strand, within nhaA at 300.017 kb on - strand, within nhaA at 300.017 kb on - strand, within nhaA at 300.061 kb on - strand, within nhaA at 300.061 kb on - strand, within nhaA at 300.121 kb on + strand, within nhaA at 300.122 kb on - strand, within nhaA at 300.223 kb on + strand, within nhaA at 300.224 kb on - strand, within nhaA at 300.253 kb on - strand, within nhaA at 300.326 kb on - strand, within nhaA at 300.326 kb on - strand, within nhaA at 300.396 kb on - strand, within nhaA at 300.396 kb on - strand, within nhaA at 300.396 kb on - strand, within nhaA at 300.411 kb on + strand, within nhaA at 300.497 kb on + strand, within nhaA at 300.505 kb on + strand, within nhaA at 300.510 kb on + strand, within nhaA at 300.526 kb on + strand, within nhaA at 300.685 kb on - strand, within nhaA at 300.685 kb on - strand, within nhaA at 300.688 kb on + strand, within nhaA at 300.689 kb on - strand, within nhaA at 300.689 kb on - strand, within nhaA at 300.700 kb on - strand, within nhaA at 300.700 kb on - strand, within nhaA at 300.985 kb on + strand at 300.985 kb on + strand at 300.985 kb on + strand at 301.039 kb on - strand at 301.057 kb on + strand at 301.058 kb on - strand at 301.058 kb on - strand at 301.069 kb on - strand at 301.076 kb on + strand at 301.109 kb on + strand at 301.207 kb on + strand at 301.208 kb on - strand at 301.218 kb on - strand at 301.228 kb on + strand at 301.252 kb on - strand at 301.252 kb on - strand at 301.264 kb on + strand at 301.272 kb on - strand at 301.318 kb on + strand at 301.338 kb on - strand at 301.354 kb on + strand at 301.354 kb on + strand at 301.370 kb on - strand at 301.381 kb on + strand at 301.457 kb on + strand at 301.457 kb on + strand at 301.459 kb on + strand at 301.459 kb on + strand at 301.534 kb on - strand at 301.559 kb on + strand at 301.559 kb on + strand at 301.560 kb on - strand at 301.561 kb on + strand at 301.562 kb on - strand at 301.562 kb on - strand at 301.562 kb on - strand at 301.576 kb on + strand at 301.576 kb on + strand at 301.577 kb on - strand at 301.577 kb on - strand at 301.577 kb on - strand at 301.630 kb on + strand, within mokC at 301.630 kb on + strand, within mokC at 301.630 kb on + strand, within mokC at 301.630 kb on + strand, within mokC at 301.630 kb on + strand, within mokC at 301.631 kb on - strand, within mokC at 301.631 kb on - strand, within mokC at 301.631 kb on - strand, within mokC at 301.631 kb on - strand, within mokC at 301.663 kb on + strand, within mokC at 301.664 kb on - strand, within mokC at 301.664 kb on - strand, within mokC at 301.675 kb on - strand, within mokC at 301.769 kb on + strand at 301.818 kb on - strand at 301.818 kb on - strand at 301.839 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 298,867 + +0.5 298,868 - -2.3 298,868 - -2.3 298,881 + +1.1 298,882 - -1.6 298,920 - +0.0 298,992 - nhaR MCAODC_01540 0.10 +1.2 298,992 - nhaR MCAODC_01540 0.10 -0.4 298,999 + nhaR MCAODC_01540 0.11 +0.6 299,054 - nhaR MCAODC_01540 0.17 -0.4 299,068 + nhaR MCAODC_01540 0.18 -1.0 299,069 - nhaR MCAODC_01540 0.19 +1.0 299,155 - nhaR MCAODC_01540 0.28 -0.3 299,160 + nhaR MCAODC_01540 0.29 +0.9 299,174 + nhaR MCAODC_01540 0.30 +0.4 299,183 + nhaR MCAODC_01540 0.31 -0.2 299,183 + nhaR MCAODC_01540 0.31 +0.5 299,184 - nhaR MCAODC_01540 0.31 -1.2 299,187 + nhaR MCAODC_01540 0.32 +0.4 299,188 - nhaR MCAODC_01540 0.32 +1.3 299,188 - nhaR MCAODC_01540 0.32 -0.8 299,280 - nhaR MCAODC_01540 0.42 -1.1 299,388 + nhaR MCAODC_01540 0.54 +0.6 299,388 + nhaR MCAODC_01540 0.54 +1.2 299,451 + nhaR MCAODC_01540 0.61 -1.9 299,451 + nhaR MCAODC_01540 0.61 -0.8 299,496 + nhaR MCAODC_01540 0.66 -1.3 299,497 - nhaR MCAODC_01540 0.66 -3.7 299,542 + nhaR MCAODC_01540 0.71 +0.1 299,576 + nhaR MCAODC_01540 0.75 -3.9 299,599 + nhaR MCAODC_01540 0.77 -0.8 299,649 - nhaR MCAODC_01540 0.83 +1.2 299,649 - nhaR MCAODC_01540 0.83 -1.0 299,652 + nhaR MCAODC_01540 0.83 +0.9 299,652 + nhaR MCAODC_01540 0.83 +2.8 299,653 - nhaR MCAODC_01540 0.83 +3.5 299,712 + +0.9 299,716 + -2.0 299,716 + -1.4 299,717 - +0.5 299,753 + +1.1 299,753 + -0.6 299,754 - +1.0 299,755 + -2.6 299,755 + +1.2 299,756 - +0.4 299,770 + +0.5 299,773 + -0.9 299,773 + +0.8 299,773 + +1.2 299,773 + +0.4 299,773 + -0.7 299,774 - -2.2 299,774 - +0.3 299,774 - +1.9 299,786 - +0.3 299,927 - -0.4 299,927 - +0.2 299,975 + -3.4 299,976 - +0.9 299,981 - +1.7 300,001 + nhaA MCAODC_01545 0.11 -2.1 300,007 + nhaA MCAODC_01545 0.12 -1.6 300,007 + nhaA MCAODC_01545 0.12 -2.5 300,007 + nhaA MCAODC_01545 0.12 +1.5 300,013 + nhaA MCAODC_01545 0.13 -1.6 300,013 + nhaA MCAODC_01545 0.13 +2.4 300,013 + nhaA MCAODC_01545 0.13 +1.7 300,013 + nhaA MCAODC_01545 0.13 -0.3 300,014 - nhaA MCAODC_01545 0.13 +1.1 300,014 - nhaA MCAODC_01545 0.13 -0.2 300,016 + nhaA MCAODC_01545 0.13 +0.4 300,017 - nhaA MCAODC_01545 0.13 +0.6 300,017 - nhaA MCAODC_01545 0.13 +2.7 300,017 - nhaA MCAODC_01545 0.13 -0.4 300,061 - nhaA MCAODC_01545 0.17 +0.4 300,061 - nhaA MCAODC_01545 0.17 -1.5 300,121 + nhaA MCAODC_01545 0.22 -0.2 300,122 - nhaA MCAODC_01545 0.22 -2.0 300,223 + nhaA MCAODC_01545 0.31 +0.6 300,224 - nhaA MCAODC_01545 0.31 -2.6 300,253 - nhaA MCAODC_01545 0.33 +0.5 300,326 - nhaA MCAODC_01545 0.39 -2.3 300,326 - nhaA MCAODC_01545 0.39 +1.0 300,396 - nhaA MCAODC_01545 0.45 -2.3 300,396 - nhaA MCAODC_01545 0.45 -2.5 300,396 - nhaA MCAODC_01545 0.45 -0.4 300,411 + nhaA MCAODC_01545 0.47 -2.8 300,497 + nhaA MCAODC_01545 0.54 -0.5 300,505 + nhaA MCAODC_01545 0.55 -3.9 300,510 + nhaA MCAODC_01545 0.55 +2.1 300,526 + nhaA MCAODC_01545 0.56 +1.6 300,685 - nhaA MCAODC_01545 0.70 +0.5 300,685 - nhaA MCAODC_01545 0.70 -1.5 300,688 + nhaA MCAODC_01545 0.70 -1.9 300,689 - nhaA MCAODC_01545 0.70 -3.5 300,689 - nhaA MCAODC_01545 0.70 -3.7 300,700 - nhaA MCAODC_01545 0.71 -0.3 300,700 - nhaA MCAODC_01545 0.71 +1.9 300,985 + -2.3 300,985 + +0.0 300,985 + +0.3 301,039 - +0.3 301,057 + -0.6 301,058 - +1.2 301,058 - +1.4 301,069 - -0.3 301,076 + -2.0 301,109 + -0.5 301,207 + +0.2 301,208 - -0.6 301,218 - +0.7 301,228 + +0.4 301,252 - -0.7 301,252 - +0.6 301,264 + -1.9 301,272 - -0.3 301,318 + -2.2 301,338 - +0.8 301,354 + +0.5 301,354 + -2.2 301,370 - -3.8 301,381 + -2.2 301,457 + +0.0 301,457 + +0.4 301,459 + +1.5 301,459 + -4.0 301,534 - -0.8 301,559 + -0.7 301,559 + +1.2 301,560 - +0.4 301,561 + +0.7 301,562 - -0.1 301,562 - -0.1 301,562 - +1.7 301,576 + +0.1 301,576 + -1.1 301,577 - +2.0 301,577 - +0.6 301,577 - -0.8 301,630 + mokC MCAODC_01555 0.32 +0.7 301,630 + mokC MCAODC_01555 0.32 +0.1 301,630 + mokC MCAODC_01555 0.32 +0.7 301,630 + mokC MCAODC_01555 0.32 +0.1 301,630 + mokC MCAODC_01555 0.32 +0.5 301,631 - mokC MCAODC_01555 0.33 +0.9 301,631 - mokC MCAODC_01555 0.33 -2.4 301,631 - mokC MCAODC_01555 0.33 +1.8 301,631 - mokC MCAODC_01555 0.33 +0.8 301,663 + mokC MCAODC_01555 0.48 -0.5 301,664 - mokC MCAODC_01555 0.49 -1.3 301,664 - mokC MCAODC_01555 0.49 -3.7 301,675 - mokC MCAODC_01555 0.54 +1.0 301,769 + -0.8 301,818 - +0.9 301,818 - -2.1 301,839 + -1.3
Or see this region's nucleotide sequence