Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt espY1 and djlA are separated by 294 nucleotides djlA and lptD are separated by 254 nucleotides
MCAODC_01325: espY1 - Type III secretion system effector EspY1, at 253,009 to 253,746
espY1
MCAODC_01330: djlA - co-chaperone DjlA, at 254,041 to 254,856
djlA
MCAODC_01335: lptD - LPS assembly protein LptD, at 255,111 to 257,465
lptD
Position (kb) 254
255 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 253.047 kb on + strand at 253.048 kb on - strand at 253.107 kb on + strand, within espY1 at 253.108 kb on - strand, within espY1 at 253.108 kb on - strand, within espY1 at 253.112 kb on - strand, within espY1 at 253.121 kb on + strand, within espY1 at 253.122 kb on - strand, within espY1 at 253.123 kb on + strand, within espY1 at 253.123 kb on + strand, within espY1 at 253.123 kb on + strand, within espY1 at 253.124 kb on - strand, within espY1 at 253.124 kb on - strand, within espY1 at 253.209 kb on + strand, within espY1 at 253.209 kb on + strand, within espY1 at 253.271 kb on + strand, within espY1 at 253.272 kb on - strand, within espY1 at 253.272 kb on - strand, within espY1 at 253.272 kb on - strand, within espY1 at 253.281 kb on + strand, within espY1 at 253.300 kb on - strand, within espY1 at 253.336 kb on + strand, within espY1 at 253.369 kb on + strand, within espY1 at 253.408 kb on - strand, within espY1 at 253.408 kb on - strand, within espY1 at 253.477 kb on - strand, within espY1 at 253.518 kb on + strand, within espY1 at 253.683 kb on + strand at 253.683 kb on + strand at 253.686 kb on - strand at 253.686 kb on - strand at 253.686 kb on - strand at 253.705 kb on - strand at 253.738 kb on + strand at 253.752 kb on + strand at 253.795 kb on - strand at 253.795 kb on - strand at 253.798 kb on + strand at 253.825 kb on + strand at 253.826 kb on - strand at 253.888 kb on - strand at 253.906 kb on - strand at 253.935 kb on + strand at 253.935 kb on + strand at 254.035 kb on - strand at 254.047 kb on - strand at 254.047 kb on - strand at 254.064 kb on + strand at 254.065 kb on - strand at 254.178 kb on + strand, within djlA at 254.179 kb on - strand, within djlA at 254.179 kb on - strand, within djlA at 254.179 kb on - strand, within djlA at 254.236 kb on - strand, within djlA at 254.236 kb on - strand, within djlA at 254.280 kb on + strand, within djlA at 254.281 kb on - strand, within djlA at 254.281 kb on - strand, within djlA at 254.374 kb on + strand, within djlA at 254.393 kb on + strand, within djlA at 254.394 kb on - strand, within djlA at 254.394 kb on - strand, within djlA at 254.394 kb on - strand, within djlA at 254.394 kb on - strand, within djlA at 254.474 kb on - strand, within djlA at 254.531 kb on + strand, within djlA at 254.532 kb on - strand, within djlA at 254.622 kb on + strand, within djlA at 254.628 kb on + strand, within djlA at 254.628 kb on + strand, within djlA at 254.629 kb on - strand, within djlA at 254.629 kb on - strand, within djlA at 254.740 kb on - strand, within djlA at 254.764 kb on - strand, within djlA at 254.764 kb on - strand, within djlA at 254.764 kb on - strand, within djlA at 254.774 kb on - strand, within djlA at 254.774 kb on - strand, within djlA at 254.782 kb on + strand at 254.783 kb on - strand at 254.783 kb on - strand at 254.816 kb on - strand at 254.816 kb on - strand at 254.850 kb on - strand at 254.856 kb on + strand at 254.856 kb on + strand at 254.856 kb on + strand at 254.856 kb on + strand at 254.856 kb on + strand at 254.856 kb on + strand at 254.856 kb on + strand at 254.857 kb on - strand at 254.857 kb on - strand at 254.857 kb on - strand at 254.857 kb on - strand at 254.858 kb on + strand at 254.858 kb on + strand at 254.858 kb on + strand at 254.859 kb on - strand at 254.859 kb on - strand at 254.859 kb on - strand at 254.859 kb on - strand at 254.859 kb on - strand at 254.859 kb on - strand at 254.859 kb on - strand at 254.859 kb on - strand at 254.859 kb on - strand at 254.859 kb on - strand at 254.890 kb on + strand at 254.902 kb on + strand at 254.903 kb on - strand at 254.903 kb on - strand at 254.903 kb on - strand at 254.904 kb on + strand at 254.904 kb on + strand at 254.904 kb on + strand at 254.904 kb on + strand at 254.905 kb on - strand at 254.905 kb on - strand at 254.905 kb on - strand at 254.905 kb on - strand at 254.905 kb on - strand at 254.905 kb on - strand at 254.955 kb on - strand at 254.956 kb on + strand at 254.966 kb on + strand at 254.971 kb on + strand at 254.971 kb on + strand at 254.971 kb on + strand at 254.971 kb on + strand at 254.971 kb on + strand at 254.972 kb on - strand at 254.972 kb on - strand at 255.009 kb on + strand at 255.023 kb on + strand at 255.023 kb on + strand at 255.034 kb on + strand at 255.047 kb on + strand at 255.047 kb on + strand at 255.047 kb on + strand at 255.047 kb on + strand at 255.052 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 253,047 + +0.1 253,048 - +0.2 253,107 + espY1 MCAODC_01325 0.13 +0.2 253,108 - espY1 MCAODC_01325 0.13 -1.5 253,108 - espY1 MCAODC_01325 0.13 -0.5 253,112 - espY1 MCAODC_01325 0.14 +0.3 253,121 + espY1 MCAODC_01325 0.15 +0.2 253,122 - espY1 MCAODC_01325 0.15 +0.9 253,123 + espY1 MCAODC_01325 0.15 -0.3 253,123 + espY1 MCAODC_01325 0.15 +0.0 253,123 + espY1 MCAODC_01325 0.15 -0.8 253,124 - espY1 MCAODC_01325 0.16 +1.2 253,124 - espY1 MCAODC_01325 0.16 -1.4 253,209 + espY1 MCAODC_01325 0.27 -3.6 253,209 + espY1 MCAODC_01325 0.27 -1.9 253,271 + espY1 MCAODC_01325 0.36 -1.6 253,272 - espY1 MCAODC_01325 0.36 -3.4 253,272 - espY1 MCAODC_01325 0.36 +2.2 253,272 - espY1 MCAODC_01325 0.36 -0.7 253,281 + espY1 MCAODC_01325 0.37 -0.6 253,300 - espY1 MCAODC_01325 0.39 -1.2 253,336 + espY1 MCAODC_01325 0.44 -0.7 253,369 + espY1 MCAODC_01325 0.49 -0.5 253,408 - espY1 MCAODC_01325 0.54 -0.0 253,408 - espY1 MCAODC_01325 0.54 -0.7 253,477 - espY1 MCAODC_01325 0.63 -3.0 253,518 + espY1 MCAODC_01325 0.69 +1.0 253,683 + -0.1 253,683 + +1.6 253,686 - -0.2 253,686 - +0.9 253,686 - -0.7 253,705 - +1.1 253,738 + +0.8 253,752 + +0.1 253,795 - -0.1 253,795 - +0.8 253,798 + +1.2 253,825 + +1.1 253,826 - -2.1 253,888 - +0.4 253,906 - -0.1 253,935 + +0.6 253,935 + -2.8 254,035 - +1.3 254,047 - +0.3 254,047 - +0.8 254,064 + +0.6 254,065 - +0.4 254,178 + djlA MCAODC_01330 0.17 +2.0 254,179 - djlA MCAODC_01330 0.17 +1.1 254,179 - djlA MCAODC_01330 0.17 -0.4 254,179 - djlA MCAODC_01330 0.17 -0.1 254,236 - djlA MCAODC_01330 0.24 -2.8 254,236 - djlA MCAODC_01330 0.24 -2.1 254,280 + djlA MCAODC_01330 0.29 -0.7 254,281 - djlA MCAODC_01330 0.29 +3.3 254,281 - djlA MCAODC_01330 0.29 +1.0 254,374 + djlA MCAODC_01330 0.41 -1.0 254,393 + djlA MCAODC_01330 0.43 +2.3 254,394 - djlA MCAODC_01330 0.43 -0.5 254,394 - djlA MCAODC_01330 0.43 +0.4 254,394 - djlA MCAODC_01330 0.43 +0.9 254,394 - djlA MCAODC_01330 0.43 +0.5 254,474 - djlA MCAODC_01330 0.53 -1.4 254,531 + djlA MCAODC_01330 0.60 -2.2 254,532 - djlA MCAODC_01330 0.60 +1.6 254,622 + djlA MCAODC_01330 0.71 +0.5 254,628 + djlA MCAODC_01330 0.72 +0.6 254,628 + djlA MCAODC_01330 0.72 +1.5 254,629 - djlA MCAODC_01330 0.72 -1.3 254,629 - djlA MCAODC_01330 0.72 -0.6 254,740 - djlA MCAODC_01330 0.86 +1.0 254,764 - djlA MCAODC_01330 0.89 +2.3 254,764 - djlA MCAODC_01330 0.89 -2.5 254,764 - djlA MCAODC_01330 0.89 +0.5 254,774 - djlA MCAODC_01330 0.90 +0.7 254,774 - djlA MCAODC_01330 0.90 +0.5 254,782 + -2.2 254,783 - -0.7 254,783 - +0.7 254,816 - -0.2 254,816 - +1.5 254,850 - +1.9 254,856 + +0.0 254,856 + +1.2 254,856 + +1.4 254,856 + -0.9 254,856 + -3.2 254,856 + +0.9 254,856 + +1.5 254,857 - +0.1 254,857 - +0.8 254,857 - -0.8 254,857 - -1.8 254,858 + +0.2 254,858 + -1.3 254,858 + +0.1 254,859 - -1.8 254,859 - +1.1 254,859 - -0.9 254,859 - -0.5 254,859 - +0.7 254,859 - -2.7 254,859 - +0.6 254,859 - -0.8 254,859 - +1.7 254,859 - +0.4 254,890 + +1.0 254,902 + +0.7 254,903 - -0.7 254,903 - -1.2 254,903 - +1.5 254,904 + -1.5 254,904 + -1.6 254,904 + +1.4 254,904 + -0.2 254,905 - -1.2 254,905 - +1.8 254,905 - +0.0 254,905 - -0.0 254,905 - -1.6 254,905 - -2.8 254,955 - +0.8 254,956 + -0.1 254,966 + -0.5 254,971 + -0.2 254,971 + -0.2 254,971 + +1.2 254,971 + +0.1 254,971 + -1.5 254,972 - +0.7 254,972 - +0.8 255,009 + -2.0 255,023 + -0.9 255,023 + +1.4 255,034 + +0.0 255,047 + -0.8 255,047 + +1.0 255,047 + +0.0 255,047 + -2.3 255,052 + -0.5
Or see this region's nucleotide sequence