Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hrpB and thpR are separated by 73 nucleotides thpR and sfsA are separated by 14 nucleotides sfsA and dksA are separated by 177 nucleotides
MCAODC_00845: hrpB - ATP-dependent helicase HrpB, at 148,354 to 150,783
hrpB
MCAODC_00850: thpR - RNA 2',3'-cyclic phosphodiesterase, at 150,857 to 151,387
thpR
MCAODC_00855: sfsA - DNA/RNA nuclease SfsA, at 151,402 to 152,106
sfsA
MCAODC_00860: dksA - RNA polymerase-binding protein DksA, at 152,284 to 152,739
dksA
Position (kb) 150
151
152 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 149.934 kb on + strand, within hrpB at 150.047 kb on + strand, within hrpB at 150.064 kb on + strand, within hrpB at 150.064 kb on + strand, within hrpB at 150.064 kb on + strand, within hrpB at 150.064 kb on + strand, within hrpB at 150.064 kb on + strand, within hrpB at 150.064 kb on + strand, within hrpB at 150.064 kb on + strand, within hrpB at 150.064 kb on + strand, within hrpB at 150.064 kb on + strand, within hrpB at 150.064 kb on + strand, within hrpB at 150.064 kb on + strand, within hrpB at 150.064 kb on + strand, within hrpB at 150.065 kb on - strand, within hrpB at 150.065 kb on - strand, within hrpB at 150.065 kb on - strand, within hrpB at 150.065 kb on - strand, within hrpB at 150.065 kb on - strand, within hrpB at 150.065 kb on - strand, within hrpB at 150.065 kb on - strand, within hrpB at 150.134 kb on - strand, within hrpB at 150.134 kb on - strand, within hrpB at 150.220 kb on + strand, within hrpB at 150.220 kb on + strand, within hrpB at 150.221 kb on - strand, within hrpB at 150.224 kb on + strand, within hrpB at 150.224 kb on + strand, within hrpB at 150.321 kb on + strand, within hrpB at 150.322 kb on - strand, within hrpB at 150.322 kb on - strand, within hrpB at 150.322 kb on - strand, within hrpB at 150.333 kb on + strand, within hrpB at 150.333 kb on + strand, within hrpB at 150.333 kb on + strand, within hrpB at 150.334 kb on - strand, within hrpB at 150.334 kb on - strand, within hrpB at 150.491 kb on - strand, within hrpB at 150.491 kb on - strand, within hrpB at 150.630 kb on + strand at 150.709 kb on + strand at 150.709 kb on + strand at 150.709 kb on + strand at 150.709 kb on + strand at 150.709 kb on + strand at 150.709 kb on + strand at 150.709 kb on + strand at 150.710 kb on - strand at 150.710 kb on - strand at 150.710 kb on - strand at 150.710 kb on - strand at 150.713 kb on - strand at 150.713 kb on - strand at 150.716 kb on - strand at 150.716 kb on - strand at 150.741 kb on + strand at 150.741 kb on + strand at 150.742 kb on - strand at 150.742 kb on - strand at 150.745 kb on + strand at 150.745 kb on + strand at 150.745 kb on + strand at 150.745 kb on + strand at 150.746 kb on - strand at 150.746 kb on - strand at 150.746 kb on - strand at 150.754 kb on + strand at 150.801 kb on + strand at 150.823 kb on + strand at 150.823 kb on + strand at 150.823 kb on + strand at 150.824 kb on - strand at 150.824 kb on - strand at 150.824 kb on - strand at 150.824 kb on - strand at 150.824 kb on - strand at 150.824 kb on - strand at 150.895 kb on - strand at 150.977 kb on - strand, within thpR at 151.168 kb on + strand, within thpR at 151.169 kb on - strand, within thpR at 151.169 kb on - strand, within thpR at 151.231 kb on + strand, within thpR at 151.231 kb on + strand, within thpR at 151.231 kb on + strand, within thpR at 151.231 kb on + strand, within thpR at 151.231 kb on + strand, within thpR at 151.231 kb on + strand, within thpR at 151.231 kb on + strand, within thpR at 151.231 kb on + strand, within thpR at 151.232 kb on - strand, within thpR at 151.232 kb on - strand, within thpR at 151.232 kb on - strand, within thpR at 151.232 kb on - strand, within thpR at 151.232 kb on - strand, within thpR at 151.232 kb on - strand, within thpR at 151.232 kb on - strand, within thpR at 151.232 kb on - strand, within thpR at 151.232 kb on - strand, within thpR at 151.234 kb on + strand, within thpR at 151.234 kb on + strand, within thpR at 151.234 kb on + strand, within thpR at 151.234 kb on + strand, within thpR at 151.234 kb on + strand, within thpR at 151.234 kb on + strand, within thpR at 151.235 kb on - strand, within thpR at 151.235 kb on - strand, within thpR at 151.235 kb on - strand, within thpR at 151.235 kb on - strand, within thpR at 151.235 kb on - strand, within thpR at 151.235 kb on - strand, within thpR at 151.235 kb on - strand, within thpR at 151.235 kb on - strand, within thpR at 151.239 kb on + strand, within thpR at 151.239 kb on + strand, within thpR at 151.240 kb on - strand, within thpR at 151.286 kb on - strand, within thpR at 151.295 kb on + strand, within thpR at 151.295 kb on + strand, within thpR at 151.296 kb on - strand, within thpR at 151.296 kb on - strand, within thpR at 151.320 kb on - strand, within thpR at 151.352 kb on + strand at 151.352 kb on + strand at 151.352 kb on + strand at 151.352 kb on + strand at 151.385 kb on + strand at 151.421 kb on + strand at 151.421 kb on + strand at 151.421 kb on + strand at 151.422 kb on - strand at 151.499 kb on + strand, within sfsA at 151.499 kb on + strand, within sfsA at 151.499 kb on + strand, within sfsA at 151.500 kb on - strand, within sfsA at 151.513 kb on - strand, within sfsA at 151.588 kb on + strand, within sfsA at 151.588 kb on + strand, within sfsA at 151.588 kb on + strand, within sfsA at 151.589 kb on - strand, within sfsA at 151.589 kb on - strand, within sfsA at 151.608 kb on - strand, within sfsA at 151.677 kb on + strand, within sfsA at 151.683 kb on + strand, within sfsA at 151.712 kb on - strand, within sfsA at 151.713 kb on + strand, within sfsA at 151.713 kb on + strand, within sfsA at 151.713 kb on + strand, within sfsA at 151.714 kb on - strand, within sfsA at 151.714 kb on - strand, within sfsA at 151.714 kb on - strand, within sfsA at 151.759 kb on - strand, within sfsA at 151.767 kb on + strand, within sfsA at 151.767 kb on + strand, within sfsA at 151.768 kb on - strand, within sfsA at 151.768 kb on - strand, within sfsA at 151.798 kb on + strand, within sfsA at 151.799 kb on - strand, within sfsA at 151.799 kb on - strand, within sfsA at 151.799 kb on - strand, within sfsA at 151.799 kb on - strand, within sfsA at 151.799 kb on - strand, within sfsA at 151.799 kb on - strand, within sfsA at 151.801 kb on - strand, within sfsA at 151.801 kb on - strand, within sfsA at 151.818 kb on + strand, within sfsA at 151.819 kb on - strand, within sfsA at 151.819 kb on - strand, within sfsA at 151.843 kb on + strand, within sfsA at 151.957 kb on - strand, within sfsA at 151.993 kb on + strand, within sfsA at 151.994 kb on - strand, within sfsA at 151.994 kb on - strand, within sfsA at 152.003 kb on + strand, within sfsA at 152.003 kb on + strand, within sfsA at 152.003 kb on + strand, within sfsA at 152.004 kb on - strand, within sfsA at 152.004 kb on - strand, within sfsA at 152.004 kb on - strand, within sfsA at 152.004 kb on - strand, within sfsA at 152.004 kb on - strand, within sfsA at 152.044 kb on + strand at 152.045 kb on - strand at 152.080 kb on - strand at 152.104 kb on + strand at 152.104 kb on + strand at 152.104 kb on + strand at 152.104 kb on + strand at 152.104 kb on + strand at 152.105 kb on - strand at 152.105 kb on - strand at 152.105 kb on - strand at 152.105 kb on - strand at 152.108 kb on - strand at 152.112 kb on + strand at 152.112 kb on + strand at 152.113 kb on - strand at 152.116 kb on + strand at 152.117 kb on - strand at 152.153 kb on - strand at 152.182 kb on + strand at 152.183 kb on - strand at 152.188 kb on + strand at 152.219 kb on - strand at 152.222 kb on + strand at 152.222 kb on + strand at 152.222 kb on + strand at 152.222 kb on + strand at 152.223 kb on - strand at 152.224 kb on + strand at 152.224 kb on + strand at 152.374 kb on + strand, within dksA at 152.374 kb on + strand, within dksA at 152.375 kb on - strand, within dksA at 152.376 kb on + strand, within dksA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 149,934 + hrpB MCAODC_00845 0.65 -0.3 150,047 + hrpB MCAODC_00845 0.70 -0.5 150,064 + hrpB MCAODC_00845 0.70 -0.9 150,064 + hrpB MCAODC_00845 0.70 -0.8 150,064 + hrpB MCAODC_00845 0.70 +0.3 150,064 + hrpB MCAODC_00845 0.70 -2.4 150,064 + hrpB MCAODC_00845 0.70 +0.8 150,064 + hrpB MCAODC_00845 0.70 -0.4 150,064 + hrpB MCAODC_00845 0.70 -0.6 150,064 + hrpB MCAODC_00845 0.70 +0.6 150,064 + hrpB MCAODC_00845 0.70 -0.7 150,064 + hrpB MCAODC_00845 0.70 +0.8 150,064 + hrpB MCAODC_00845 0.70 +2.0 150,064 + hrpB MCAODC_00845 0.70 +1.1 150,065 - hrpB MCAODC_00845 0.70 +0.5 150,065 - hrpB MCAODC_00845 0.70 -0.7 150,065 - hrpB MCAODC_00845 0.70 -0.9 150,065 - hrpB MCAODC_00845 0.70 +0.8 150,065 - hrpB MCAODC_00845 0.70 +0.7 150,065 - hrpB MCAODC_00845 0.70 +1.0 150,065 - hrpB MCAODC_00845 0.70 +0.7 150,134 - hrpB MCAODC_00845 0.73 -0.6 150,134 - hrpB MCAODC_00845 0.73 +0.6 150,220 + hrpB MCAODC_00845 0.77 -1.2 150,220 + hrpB MCAODC_00845 0.77 +1.5 150,221 - hrpB MCAODC_00845 0.77 -1.2 150,224 + hrpB MCAODC_00845 0.77 -2.3 150,224 + hrpB MCAODC_00845 0.77 -0.1 150,321 + hrpB MCAODC_00845 0.81 +0.8 150,322 - hrpB MCAODC_00845 0.81 +0.5 150,322 - hrpB MCAODC_00845 0.81 +0.3 150,322 - hrpB MCAODC_00845 0.81 +0.3 150,333 + hrpB MCAODC_00845 0.81 +0.1 150,333 + hrpB MCAODC_00845 0.81 +0.8 150,333 + hrpB MCAODC_00845 0.81 +1.3 150,334 - hrpB MCAODC_00845 0.81 +0.7 150,334 - hrpB MCAODC_00845 0.81 -0.3 150,491 - hrpB MCAODC_00845 0.88 -1.3 150,491 - hrpB MCAODC_00845 0.88 -1.9 150,630 + +0.5 150,709 + +3.5 150,709 + +1.3 150,709 + +0.6 150,709 + +0.0 150,709 + -2.4 150,709 + -1.1 150,709 + +0.1 150,710 - -2.6 150,710 - +1.8 150,710 - +0.8 150,710 - -0.0 150,713 - +0.5 150,713 - -3.2 150,716 - -2.8 150,716 - -0.3 150,741 + +0.2 150,741 + -0.1 150,742 - -0.6 150,742 - -0.0 150,745 + +0.8 150,745 + +1.3 150,745 + +1.5 150,745 + +0.1 150,746 - -2.6 150,746 - -1.5 150,746 - -0.1 150,754 + -2.2 150,801 + -1.8 150,823 + -2.8 150,823 + -0.5 150,823 + -0.7 150,824 - +0.7 150,824 - +0.5 150,824 - +0.7 150,824 - +0.2 150,824 - +0.2 150,824 - +1.2 150,895 - +0.1 150,977 - thpR MCAODC_00850 0.23 -1.6 151,168 + thpR MCAODC_00850 0.59 -0.4 151,169 - thpR MCAODC_00850 0.59 -1.2 151,169 - thpR MCAODC_00850 0.59 -1.4 151,231 + thpR MCAODC_00850 0.70 -0.8 151,231 + thpR MCAODC_00850 0.70 -1.1 151,231 + thpR MCAODC_00850 0.70 +0.1 151,231 + thpR MCAODC_00850 0.70 -1.4 151,231 + thpR MCAODC_00850 0.70 -0.0 151,231 + thpR MCAODC_00850 0.70 -1.5 151,231 + thpR MCAODC_00850 0.70 -1.7 151,231 + thpR MCAODC_00850 0.70 +0.9 151,232 - thpR MCAODC_00850 0.71 +2.9 151,232 - thpR MCAODC_00850 0.71 -2.2 151,232 - thpR MCAODC_00850 0.71 -1.4 151,232 - thpR MCAODC_00850 0.71 +0.3 151,232 - thpR MCAODC_00850 0.71 -0.2 151,232 - thpR MCAODC_00850 0.71 -3.1 151,232 - thpR MCAODC_00850 0.71 -0.3 151,232 - thpR MCAODC_00850 0.71 +0.3 151,232 - thpR MCAODC_00850 0.71 +0.6 151,234 + thpR MCAODC_00850 0.71 +0.3 151,234 + thpR MCAODC_00850 0.71 +0.5 151,234 + thpR MCAODC_00850 0.71 -0.2 151,234 + thpR MCAODC_00850 0.71 +0.0 151,234 + thpR MCAODC_00850 0.71 -0.8 151,234 + thpR MCAODC_00850 0.71 -1.5 151,235 - thpR MCAODC_00850 0.71 -1.7 151,235 - thpR MCAODC_00850 0.71 +0.0 151,235 - thpR MCAODC_00850 0.71 +0.0 151,235 - thpR MCAODC_00850 0.71 +0.3 151,235 - thpR MCAODC_00850 0.71 -0.3 151,235 - thpR MCAODC_00850 0.71 -0.1 151,235 - thpR MCAODC_00850 0.71 -0.4 151,235 - thpR MCAODC_00850 0.71 +1.1 151,239 + thpR MCAODC_00850 0.72 +0.4 151,239 + thpR MCAODC_00850 0.72 +0.8 151,240 - thpR MCAODC_00850 0.72 -0.0 151,286 - thpR MCAODC_00850 0.81 +0.6 151,295 + thpR MCAODC_00850 0.82 -0.1 151,295 + thpR MCAODC_00850 0.82 +1.7 151,296 - thpR MCAODC_00850 0.83 -0.2 151,296 - thpR MCAODC_00850 0.83 +0.2 151,320 - thpR MCAODC_00850 0.87 +1.2 151,352 + +1.0 151,352 + -1.1 151,352 + -1.7 151,352 + +0.9 151,385 + -1.7 151,421 + +1.3 151,421 + -0.2 151,421 + -0.1 151,422 - +1.7 151,499 + sfsA MCAODC_00855 0.14 -0.6 151,499 + sfsA MCAODC_00855 0.14 +0.6 151,499 + sfsA MCAODC_00855 0.14 -1.4 151,500 - sfsA MCAODC_00855 0.14 +1.7 151,513 - sfsA MCAODC_00855 0.16 -2.5 151,588 + sfsA MCAODC_00855 0.26 -0.4 151,588 + sfsA MCAODC_00855 0.26 -0.2 151,588 + sfsA MCAODC_00855 0.26 -3.1 151,589 - sfsA MCAODC_00855 0.27 +0.5 151,589 - sfsA MCAODC_00855 0.27 -0.5 151,608 - sfsA MCAODC_00855 0.29 +0.2 151,677 + sfsA MCAODC_00855 0.39 -1.9 151,683 + sfsA MCAODC_00855 0.40 +1.0 151,712 - sfsA MCAODC_00855 0.44 +2.1 151,713 + sfsA MCAODC_00855 0.44 -2.8 151,713 + sfsA MCAODC_00855 0.44 -1.0 151,713 + sfsA MCAODC_00855 0.44 +0.4 151,714 - sfsA MCAODC_00855 0.44 -2.6 151,714 - sfsA MCAODC_00855 0.44 -1.7 151,714 - sfsA MCAODC_00855 0.44 -0.3 151,759 - sfsA MCAODC_00855 0.51 +2.2 151,767 + sfsA MCAODC_00855 0.52 +0.2 151,767 + sfsA MCAODC_00855 0.52 -0.8 151,768 - sfsA MCAODC_00855 0.52 +0.8 151,768 - sfsA MCAODC_00855 0.52 -0.6 151,798 + sfsA MCAODC_00855 0.56 +0.3 151,799 - sfsA MCAODC_00855 0.56 +0.7 151,799 - sfsA MCAODC_00855 0.56 +1.4 151,799 - sfsA MCAODC_00855 0.56 -0.9 151,799 - sfsA MCAODC_00855 0.56 +0.6 151,799 - sfsA MCAODC_00855 0.56 +0.2 151,799 - sfsA MCAODC_00855 0.56 -0.3 151,801 - sfsA MCAODC_00855 0.57 +2.6 151,801 - sfsA MCAODC_00855 0.57 +1.0 151,818 + sfsA MCAODC_00855 0.59 -0.0 151,819 - sfsA MCAODC_00855 0.59 +0.6 151,819 - sfsA MCAODC_00855 0.59 -0.5 151,843 + sfsA MCAODC_00855 0.63 +1.6 151,957 - sfsA MCAODC_00855 0.79 -2.4 151,993 + sfsA MCAODC_00855 0.84 -0.0 151,994 - sfsA MCAODC_00855 0.84 -1.6 151,994 - sfsA MCAODC_00855 0.84 +0.6 152,003 + sfsA MCAODC_00855 0.85 -0.3 152,003 + sfsA MCAODC_00855 0.85 -0.5 152,003 + sfsA MCAODC_00855 0.85 +0.8 152,004 - sfsA MCAODC_00855 0.85 +1.2 152,004 - sfsA MCAODC_00855 0.85 +0.8 152,004 - sfsA MCAODC_00855 0.85 +0.5 152,004 - sfsA MCAODC_00855 0.85 -0.7 152,004 - sfsA MCAODC_00855 0.85 -0.5 152,044 + +0.3 152,045 - +0.3 152,080 - +1.9 152,104 + -0.6 152,104 + -3.4 152,104 + +0.0 152,104 + -1.4 152,104 + -0.3 152,105 - -0.9 152,105 - +0.3 152,105 - -0.4 152,105 - -0.7 152,108 - -1.3 152,112 + +0.6 152,112 + -0.6 152,113 - +1.9 152,116 + +0.9 152,117 - +0.5 152,153 - +0.4 152,182 + +1.9 152,183 - -0.2 152,188 + +0.1 152,219 - -0.7 152,222 + -0.3 152,222 + -0.7 152,222 + -0.1 152,222 + -0.7 152,223 - -3.2 152,224 + -0.1 152,224 + -3.8 152,374 + dksA MCAODC_00860 0.20 -4.2 152,374 + dksA MCAODC_00860 0.20 -2.7 152,375 - dksA MCAODC_00860 0.20 -1.6 152,376 + dksA MCAODC_00860 0.20 -0.1
Or see this region's nucleotide sequence