Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rcsF and tsaA overlap by 4 nucleotides tsaA and proS are separated by 110 nucleotides proS and yaeF are separated by 52 nucleotides
MCAODC_00580: rcsF - Rcs stress response system protein RcsF, at 93,885 to 94,289
rcsF
MCAODC_00585: tsaA - tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO, at 94,286 to 94,993
tsaA
MCAODC_00590: proS - proline--tRNA ligase, at 95,104 to 96,822
proS
MCAODC_00595: yaeF - YaeF family permuted papain-like enzyme, at 96,875 to 97,699
yaeF
Position (kb) 95
96
97 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 94.195 kb on + strand, within rcsF at 94.195 kb on + strand, within rcsF at 94.198 kb on + strand, within rcsF at 94.198 kb on + strand, within rcsF at 94.198 kb on + strand, within rcsF at 94.198 kb on + strand, within rcsF at 94.199 kb on - strand, within rcsF at 94.199 kb on - strand, within rcsF at 94.199 kb on - strand, within rcsF at 94.199 kb on - strand, within rcsF at 94.205 kb on + strand, within rcsF at 94.205 kb on + strand, within rcsF at 94.206 kb on - strand, within rcsF at 94.217 kb on + strand, within rcsF at 94.240 kb on - strand, within rcsF at 94.252 kb on + strand at 94.252 kb on + strand at 94.253 kb on - strand at 94.253 kb on - strand at 94.256 kb on + strand at 94.271 kb on + strand at 94.277 kb on + strand at 94.278 kb on - strand at 94.311 kb on + strand at 94.311 kb on + strand at 94.312 kb on - strand at 94.319 kb on - strand at 94.332 kb on - strand at 94.332 kb on - strand at 94.332 kb on - strand at 94.392 kb on + strand, within tsaA at 94.392 kb on + strand, within tsaA at 94.392 kb on + strand, within tsaA at 94.392 kb on + strand, within tsaA at 94.393 kb on - strand, within tsaA at 94.393 kb on - strand, within tsaA at 94.409 kb on + strand, within tsaA at 94.409 kb on + strand, within tsaA at 94.410 kb on - strand, within tsaA at 94.410 kb on - strand, within tsaA at 94.589 kb on - strand, within tsaA at 94.589 kb on - strand, within tsaA at 94.589 kb on - strand, within tsaA at 94.672 kb on + strand, within tsaA at 94.673 kb on - strand, within tsaA at 94.697 kb on + strand, within tsaA at 94.698 kb on - strand, within tsaA at 94.739 kb on + strand, within tsaA at 94.739 kb on + strand, within tsaA at 94.739 kb on + strand, within tsaA at 94.739 kb on + strand, within tsaA at 94.740 kb on - strand, within tsaA at 94.740 kb on - strand, within tsaA at 94.833 kb on + strand, within tsaA at 94.855 kb on - strand, within tsaA at 94.885 kb on + strand, within tsaA at 94.886 kb on - strand, within tsaA at 94.924 kb on - strand at 96.813 kb on + strand at 96.881 kb on - strand at 96.893 kb on + strand at 96.937 kb on + strand at 96.978 kb on + strand, within yaeF at 96.979 kb on - strand, within yaeF at 96.979 kb on - strand, within yaeF at 97.045 kb on - strand, within yaeF at 97.045 kb on - strand, within yaeF at 97.045 kb on - strand, within yaeF at 97.054 kb on - strand, within yaeF at 97.059 kb on + strand, within yaeF at 97.163 kb on + strand, within yaeF at 97.163 kb on + strand, within yaeF at 97.164 kb on - strand, within yaeF at 97.178 kb on - strand, within yaeF at 97.240 kb on + strand, within yaeF at 97.240 kb on + strand, within yaeF at 97.241 kb on - strand, within yaeF at 97.241 kb on - strand, within yaeF at 97.241 kb on - strand, within yaeF at 97.261 kb on - strand, within yaeF at 97.261 kb on - strand, within yaeF at 97.327 kb on + strand, within yaeF at 97.328 kb on - strand, within yaeF at 97.328 kb on - strand, within yaeF at 97.334 kb on + strand, within yaeF at 97.337 kb on - strand, within yaeF at 97.337 kb on - strand, within yaeF at 97.340 kb on + strand, within yaeF at 97.340 kb on + strand, within yaeF at 97.370 kb on - strand, within yaeF at 97.370 kb on - strand, within yaeF at 97.565 kb on - strand, within yaeF at 97.585 kb on + strand, within yaeF at 97.585 kb on + strand, within yaeF at 97.585 kb on + strand, within yaeF at 97.586 kb on - strand, within yaeF at 97.586 kb on - strand, within yaeF at 97.586 kb on - strand, within yaeF at 97.586 kb on - strand, within yaeF at 97.592 kb on - strand, within yaeF at 97.629 kb on + strand at 97.630 kb on - strand at 97.630 kb on - strand at 97.634 kb on + strand at 97.634 kb on + strand at 97.635 kb on - strand at 97.661 kb on + strand at 97.661 kb on + strand at 97.661 kb on + strand at 97.662 kb on - strand at 97.662 kb on - strand at 97.701 kb on - strand at 97.705 kb on + strand at 97.706 kb on - strand at 97.706 kb on - strand at 97.706 kb on - strand at 97.723 kb on + strand at 97.728 kb on + strand at 97.728 kb on + strand at 97.728 kb on + strand at 97.728 kb on + strand at 97.743 kb on - strand at 97.752 kb on - strand at 97.752 kb on - strand at 97.752 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 94,195 + rcsF MCAODC_00580 0.77 -0.3 94,195 + rcsF MCAODC_00580 0.77 +1.9 94,198 + rcsF MCAODC_00580 0.77 +1.1 94,198 + rcsF MCAODC_00580 0.77 -0.0 94,198 + rcsF MCAODC_00580 0.77 +1.2 94,198 + rcsF MCAODC_00580 0.77 -1.3 94,199 - rcsF MCAODC_00580 0.78 -0.5 94,199 - rcsF MCAODC_00580 0.78 +2.3 94,199 - rcsF MCAODC_00580 0.78 -3.8 94,199 - rcsF MCAODC_00580 0.78 +1.0 94,205 + rcsF MCAODC_00580 0.79 +0.5 94,205 + rcsF MCAODC_00580 0.79 +1.1 94,206 - rcsF MCAODC_00580 0.79 +0.8 94,217 + rcsF MCAODC_00580 0.82 +2.8 94,240 - rcsF MCAODC_00580 0.88 +0.1 94,252 + -1.3 94,252 + -2.7 94,253 - +0.7 94,253 - -2.7 94,256 + -0.6 94,271 + +1.0 94,277 + +0.3 94,278 - +0.7 94,311 + -0.3 94,311 + -0.3 94,312 - -0.6 94,319 - +0.5 94,332 - -0.4 94,332 - +1.5 94,332 - -2.3 94,392 + tsaA MCAODC_00585 0.15 -3.1 94,392 + tsaA MCAODC_00585 0.15 -0.6 94,392 + tsaA MCAODC_00585 0.15 -2.1 94,392 + tsaA MCAODC_00585 0.15 +0.8 94,393 - tsaA MCAODC_00585 0.15 +0.4 94,393 - tsaA MCAODC_00585 0.15 +3.5 94,409 + tsaA MCAODC_00585 0.17 -1.0 94,409 + tsaA MCAODC_00585 0.17 -1.4 94,410 - tsaA MCAODC_00585 0.18 +0.9 94,410 - tsaA MCAODC_00585 0.18 -2.0 94,589 - tsaA MCAODC_00585 0.43 +1.4 94,589 - tsaA MCAODC_00585 0.43 -0.2 94,589 - tsaA MCAODC_00585 0.43 -0.6 94,672 + tsaA MCAODC_00585 0.55 -0.7 94,673 - tsaA MCAODC_00585 0.55 -0.5 94,697 + tsaA MCAODC_00585 0.58 +0.4 94,698 - tsaA MCAODC_00585 0.58 +1.0 94,739 + tsaA MCAODC_00585 0.64 -0.6 94,739 + tsaA MCAODC_00585 0.64 +0.5 94,739 + tsaA MCAODC_00585 0.64 +1.1 94,739 + tsaA MCAODC_00585 0.64 -3.7 94,740 - tsaA MCAODC_00585 0.64 -1.8 94,740 - tsaA MCAODC_00585 0.64 -1.9 94,833 + tsaA MCAODC_00585 0.77 -1.0 94,855 - tsaA MCAODC_00585 0.80 -2.0 94,885 + tsaA MCAODC_00585 0.85 -1.8 94,886 - tsaA MCAODC_00585 0.85 +2.0 94,924 - +0.3 96,813 + +0.4 96,881 - +0.8 96,893 + +0.1 96,937 + +1.2 96,978 + yaeF MCAODC_00595 0.12 +1.9 96,979 - yaeF MCAODC_00595 0.13 +0.4 96,979 - yaeF MCAODC_00595 0.13 -2.2 97,045 - yaeF MCAODC_00595 0.21 +0.6 97,045 - yaeF MCAODC_00595 0.21 +0.4 97,045 - yaeF MCAODC_00595 0.21 -3.2 97,054 - yaeF MCAODC_00595 0.22 -1.6 97,059 + yaeF MCAODC_00595 0.22 +1.8 97,163 + yaeF MCAODC_00595 0.35 -2.8 97,163 + yaeF MCAODC_00595 0.35 -0.8 97,164 - yaeF MCAODC_00595 0.35 -2.1 97,178 - yaeF MCAODC_00595 0.37 +1.8 97,240 + yaeF MCAODC_00595 0.44 +0.6 97,240 + yaeF MCAODC_00595 0.44 -0.2 97,241 - yaeF MCAODC_00595 0.44 +2.1 97,241 - yaeF MCAODC_00595 0.44 -0.4 97,241 - yaeF MCAODC_00595 0.44 +0.7 97,261 - yaeF MCAODC_00595 0.47 -3.5 97,261 - yaeF MCAODC_00595 0.47 -0.1 97,327 + yaeF MCAODC_00595 0.55 +1.1 97,328 - yaeF MCAODC_00595 0.55 -3.1 97,328 - yaeF MCAODC_00595 0.55 +1.7 97,334 + yaeF MCAODC_00595 0.56 +0.2 97,337 - yaeF MCAODC_00595 0.56 -3.8 97,337 - yaeF MCAODC_00595 0.56 -1.0 97,340 + yaeF MCAODC_00595 0.56 -0.3 97,340 + yaeF MCAODC_00595 0.56 -0.1 97,370 - yaeF MCAODC_00595 0.60 -0.1 97,370 - yaeF MCAODC_00595 0.60 +1.3 97,565 - yaeF MCAODC_00595 0.84 -0.7 97,585 + yaeF MCAODC_00595 0.86 +0.6 97,585 + yaeF MCAODC_00595 0.86 +1.8 97,585 + yaeF MCAODC_00595 0.86 +1.3 97,586 - yaeF MCAODC_00595 0.86 -0.6 97,586 - yaeF MCAODC_00595 0.86 +0.7 97,586 - yaeF MCAODC_00595 0.86 +1.2 97,586 - yaeF MCAODC_00595 0.86 -0.3 97,592 - yaeF MCAODC_00595 0.87 -0.5 97,629 + +1.7 97,630 - +0.1 97,630 - +2.2 97,634 + +2.0 97,634 + -2.3 97,635 - -3.2 97,661 + -0.6 97,661 + +0.3 97,661 + +1.2 97,662 - -3.1 97,662 - -2.7 97,701 - +2.0 97,705 + +1.1 97,706 - +0.1 97,706 - +0.2 97,706 - -1.5 97,723 + +1.3 97,728 + +0.5 97,728 + -0.7 97,728 + +1.7 97,728 + +0.2 97,743 - -0.3 97,752 - -3.1 97,752 - +0.5 97,752 - -2.4
Or see this region's nucleotide sequence