Strain Fitness in Escherichia coli ECOR38 around HEPCGN_16995

Experiment: Bas38

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntinfB and rbfA are separated by 163 nucleotidesrbfA and truB overlap by 1 nucleotides HEPCGN_16985: infB - Translation initiation factor IF-2, at 2,145,829 to 2,148,501 infB HEPCGN_16995: rbfA - 30S ribosome-binding factor RbfA, at 2,148,665 to 2,149,066 rbfA HEPCGN_17000: truB - tRNA pseudouridine(55) synthase TruB, at 2,149,066 to 2,150,010 truB Position (kb) 2148 2149 2150Strain fitness (log2 ratio) -3 -2 -1 0 1at 2148.489 kb on + strandat 2148.499 kb on + strandat 2149.120 kb on - strandat 2149.171 kb on + strand, within truBat 2149.172 kb on - strand, within truBat 2149.268 kb on - strand, within truBat 2149.456 kb on + strand, within truBat 2149.457 kb on - strand, within truBat 2149.480 kb on + strand, within truBat 2149.545 kb on + strand, within truBat 2149.545 kb on + strand, within truBat 2149.600 kb on + strand, within truBat 2149.600 kb on + strand, within truBat 2149.600 kb on + strand, within truBat 2149.600 kb on + strand, within truBat 2149.600 kb on + strand, within truBat 2149.600 kb on + strand, within truBat 2149.601 kb on - strand, within truBat 2149.601 kb on - strand, within truBat 2149.601 kb on - strand, within truBat 2149.656 kb on + strand, within truBat 2149.656 kb on + strand, within truBat 2149.657 kb on - strand, within truBat 2149.657 kb on - strand, within truBat 2149.683 kb on + strand, within truBat 2149.684 kb on - strand, within truBat 2149.684 kb on - strand, within truBat 2149.772 kb on + strand, within truBat 2149.772 kb on + strand, within truBat 2149.878 kb on + strand, within truB

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas38
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2,148,489 + -0.1
2,148,499 + -0.1
2,149,120 - -0.6
2,149,171 + truB HEPCGN_17000 0.11 -2.0
2,149,172 - truB HEPCGN_17000 0.11 -0.4
2,149,268 - truB HEPCGN_17000 0.21 -0.0
2,149,456 + truB HEPCGN_17000 0.41 +0.7
2,149,457 - truB HEPCGN_17000 0.41 -0.3
2,149,480 + truB HEPCGN_17000 0.44 +1.4
2,149,545 + truB HEPCGN_17000 0.51 +0.2
2,149,545 + truB HEPCGN_17000 0.51 -0.0
2,149,600 + truB HEPCGN_17000 0.57 -0.0
2,149,600 + truB HEPCGN_17000 0.57 -0.6
2,149,600 + truB HEPCGN_17000 0.57 +0.7
2,149,600 + truB HEPCGN_17000 0.57 +0.4
2,149,600 + truB HEPCGN_17000 0.57 -0.2
2,149,600 + truB HEPCGN_17000 0.57 -3.0
2,149,601 - truB HEPCGN_17000 0.57 -0.6
2,149,601 - truB HEPCGN_17000 0.57 -0.3
2,149,601 - truB HEPCGN_17000 0.57 +0.1
2,149,656 + truB HEPCGN_17000 0.62 +0.6
2,149,656 + truB HEPCGN_17000 0.62 -0.5
2,149,657 - truB HEPCGN_17000 0.63 -0.5
2,149,657 - truB HEPCGN_17000 0.63 +1.0
2,149,683 + truB HEPCGN_17000 0.65 +0.2
2,149,684 - truB HEPCGN_17000 0.65 -0.3
2,149,684 - truB HEPCGN_17000 0.65 -0.1
2,149,772 + truB HEPCGN_17000 0.75 -2.2
2,149,772 + truB HEPCGN_17000 0.75 +1.0
2,149,878 + truB HEPCGN_17000 0.86 +0.4

Or see this region's nucleotide sequence