Experiment: Bas38
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yiiR and yiiQ are separated by 100 nucleotides yiiQ and tpiA are separated by 107 nucleotides tpiA and cdh are separated by 54 nucleotides cdh and sbp are separated by 106 nucleotides
HEPCGN_12445: yiiR - Uncharacterized protein YiiR, at 1,238,451 to 1,238,891
yiiR
HEPCGN_12450: yiiQ - Uncharacterized protein YiiQ, at 1,238,992 to 1,239,591
yiiQ
HEPCGN_12455: tpiA - triose-phosphate isomerase, at 1,239,699 to 1,240,466
tpiA
HEPCGN_12460: cdh - CDP-diacylglycerol diphosphatase, at 1,240,521 to 1,241,276
cdh
HEPCGN_12465: sbp - sulfate/thiosulfate ABC transporter substrate-binding protein Sbp, at 1,241,383 to 1,242,372
sbp
Position (kb)
1239
1240
1241 Strain fitness (log2 ratio)
-2
-1
0
1 at 1238.853 kb on - strand at 1238.861 kb on + strand at 1238.862 kb on - strand at 1238.862 kb on - strand at 1238.872 kb on - strand at 1238.884 kb on - strand at 1238.884 kb on - strand at 1238.893 kb on + strand at 1238.894 kb on - strand at 1238.926 kb on + strand at 1238.927 kb on - strand at 1238.974 kb on + strand at 1238.985 kb on - strand at 1239.006 kb on + strand at 1239.023 kb on + strand at 1239.023 kb on + strand at 1239.023 kb on + strand at 1239.024 kb on - strand at 1239.026 kb on - strand at 1239.150 kb on + strand, within yiiQ at 1239.257 kb on - strand, within yiiQ at 1239.257 kb on - strand, within yiiQ at 1239.257 kb on - strand, within yiiQ at 1239.257 kb on - strand, within yiiQ at 1239.265 kb on - strand, within yiiQ at 1239.440 kb on + strand, within yiiQ at 1239.444 kb on - strand, within yiiQ at 1239.466 kb on + strand, within yiiQ at 1239.470 kb on - strand, within yiiQ at 1239.470 kb on - strand, within yiiQ at 1239.497 kb on - strand, within yiiQ at 1239.625 kb on - strand at 1239.680 kb on - strand at 1239.680 kb on - strand at 1239.695 kb on + strand at 1240.204 kb on + strand, within tpiA at 1240.465 kb on - strand at 1240.465 kb on - strand at 1240.465 kb on - strand at 1240.501 kb on - strand at 1240.501 kb on - strand at 1240.501 kb on - strand at 1240.501 kb on - strand at 1240.584 kb on + strand at 1240.648 kb on + strand, within cdh at 1240.658 kb on + strand, within cdh at 1240.698 kb on - strand, within cdh at 1240.698 kb on - strand, within cdh at 1240.747 kb on + strand, within cdh at 1240.850 kb on + strand, within cdh at 1240.850 kb on + strand, within cdh at 1241.024 kb on + strand, within cdh at 1241.033 kb on - strand, within cdh at 1241.050 kb on + strand, within cdh at 1241.171 kb on + strand, within cdh at 1241.172 kb on - strand, within cdh at 1241.175 kb on + strand, within cdh at 1241.176 kb on - strand, within cdh at 1241.176 kb on - strand, within cdh
Per-strain Table
Position Strand Gene LocusTag Fraction Bas38 remove 1,238,853 - +0.3 1,238,861 + +0.3 1,238,862 - -0.3 1,238,862 - +1.1 1,238,872 - +0.1 1,238,884 - +0.3 1,238,884 - +0.2 1,238,893 + +0.2 1,238,894 - +0.1 1,238,926 + +0.1 1,238,927 - +0.8 1,238,974 + +0.2 1,238,985 - +0.6 1,239,006 + -0.2 1,239,023 + -0.5 1,239,023 + -1.2 1,239,023 + -0.7 1,239,024 - +0.2 1,239,026 - +0.3 1,239,150 + yiiQ HEPCGN_12450 0.26 -0.6 1,239,257 - yiiQ HEPCGN_12450 0.44 +0.6 1,239,257 - yiiQ HEPCGN_12450 0.44 -2.0 1,239,257 - yiiQ HEPCGN_12450 0.44 -0.4 1,239,257 - yiiQ HEPCGN_12450 0.44 +0.1 1,239,265 - yiiQ HEPCGN_12450 0.46 -0.4 1,239,440 + yiiQ HEPCGN_12450 0.75 +0.2 1,239,444 - yiiQ HEPCGN_12450 0.75 +1.1 1,239,466 + yiiQ HEPCGN_12450 0.79 -0.4 1,239,470 - yiiQ HEPCGN_12450 0.80 +0.2 1,239,470 - yiiQ HEPCGN_12450 0.80 -0.3 1,239,497 - yiiQ HEPCGN_12450 0.84 +1.5 1,239,625 - -0.2 1,239,680 - -0.5 1,239,680 - -1.7 1,239,695 + +0.8 1,240,204 + tpiA HEPCGN_12455 0.66 +0.3 1,240,465 - -0.7 1,240,465 - +0.6 1,240,465 - +0.2 1,240,501 - -0.1 1,240,501 - -0.1 1,240,501 - -0.4 1,240,501 - +0.4 1,240,584 + +0.5 1,240,648 + cdh HEPCGN_12460 0.17 +0.5 1,240,658 + cdh HEPCGN_12460 0.18 -0.2 1,240,698 - cdh HEPCGN_12460 0.23 -0.1 1,240,698 - cdh HEPCGN_12460 0.23 -1.7 1,240,747 + cdh HEPCGN_12460 0.30 -0.5 1,240,850 + cdh HEPCGN_12460 0.44 -0.5 1,240,850 + cdh HEPCGN_12460 0.44 +0.7 1,241,024 + cdh HEPCGN_12460 0.67 -0.1 1,241,033 - cdh HEPCGN_12460 0.68 +0.5 1,241,050 + cdh HEPCGN_12460 0.70 +0.3 1,241,171 + cdh HEPCGN_12460 0.86 -2.2 1,241,172 - cdh HEPCGN_12460 0.86 -0.6 1,241,175 + cdh HEPCGN_12460 0.87 -1.1 1,241,176 - cdh HEPCGN_12460 0.87 -0.2 1,241,176 - cdh HEPCGN_12460 0.87 +0.5
Or see this region's nucleotide sequence