Experiment: Guanosine 0.35306 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS05905 and A4249_RS05910 overlap by 4 nucleotides A4249_RS05910 and A4249_RS05915 overlap by 4 nucleotides A4249_RS05915 and A4249_RS05920 are separated by 39 nucleotides A4249_RS05920 and A4249_RS05925 are separated by 101 nucleotides A4249_RS05925 and A4249_RS05930 are separated by 2 nucleotides
A4249_RS05905: A4249_RS05905 - class I poly(R)-hydroxyalkanoic acid synthase, at 1,207,992 to 1,209,932
_RS05905
A4249_RS05910: A4249_RS05910 - hypothetical protein, at 1,209,929 to 1,210,306
_RS05910
A4249_RS05915: A4249_RS05915 - peptide-methionine (R)-S-oxide reductase MsrB, at 1,210,303 to 1,210,806
_RS05915
A4249_RS05920: A4249_RS05920 - N-acetyl-gamma-glutamyl-phosphate reductase, at 1,210,846 to 1,211,796
_RS05920
A4249_RS05925: A4249_RS05925 - 30S ribosomal protein S9, at 1,211,898 to 1,212,392
_RS05925
A4249_RS05930: A4249_RS05930 - 50S ribosomal protein L13, at 1,212,395 to 1,212,871
_RS05930
Position (kb)
1210
1211
1212 Strain fitness (log2 ratio)
-2
-1
0
1 at 1209.912 kb on + strand at 1209.912 kb on + strand at 1209.912 kb on + strand at 1209.913 kb on - strand at 1209.913 kb on - strand at 1209.913 kb on - strand at 1209.913 kb on - strand at 1209.913 kb on - strand at 1210.037 kb on + strand, within A4249_RS05910 at 1210.037 kb on + strand, within A4249_RS05910 at 1210.037 kb on + strand, within A4249_RS05910 at 1210.037 kb on + strand, within A4249_RS05910 at 1210.037 kb on + strand, within A4249_RS05910 at 1210.037 kb on + strand, within A4249_RS05910 at 1210.038 kb on - strand, within A4249_RS05910 at 1210.038 kb on - strand, within A4249_RS05910 at 1210.038 kb on - strand, within A4249_RS05910 at 1210.038 kb on - strand, within A4249_RS05910 at 1210.038 kb on - strand, within A4249_RS05910 at 1210.038 kb on - strand, within A4249_RS05910 at 1210.038 kb on - strand, within A4249_RS05910 at 1210.038 kb on - strand, within A4249_RS05910 at 1210.038 kb on - strand, within A4249_RS05910 at 1210.150 kb on + strand, within A4249_RS05910 at 1210.150 kb on + strand, within A4249_RS05910 at 1210.150 kb on + strand, within A4249_RS05910 at 1210.151 kb on - strand, within A4249_RS05910 at 1210.151 kb on - strand, within A4249_RS05910 at 1210.179 kb on + strand, within A4249_RS05910 at 1210.179 kb on + strand, within A4249_RS05910 at 1210.179 kb on + strand, within A4249_RS05910 at 1210.180 kb on - strand, within A4249_RS05910 at 1210.229 kb on + strand, within A4249_RS05910 at 1210.229 kb on + strand, within A4249_RS05910 at 1210.230 kb on - strand, within A4249_RS05910 at 1210.415 kb on + strand, within A4249_RS05915 at 1210.415 kb on + strand, within A4249_RS05915 at 1210.415 kb on + strand, within A4249_RS05915 at 1210.416 kb on - strand, within A4249_RS05915 at 1210.416 kb on - strand, within A4249_RS05915 at 1210.416 kb on - strand, within A4249_RS05915 at 1210.416 kb on - strand, within A4249_RS05915 at 1210.481 kb on + strand, within A4249_RS05915 at 1210.481 kb on + strand, within A4249_RS05915 at 1210.481 kb on + strand, within A4249_RS05915 at 1210.481 kb on + strand, within A4249_RS05915 at 1210.481 kb on + strand, within A4249_RS05915 at 1210.481 kb on + strand, within A4249_RS05915 at 1210.481 kb on + strand, within A4249_RS05915 at 1210.481 kb on + strand, within A4249_RS05915 at 1210.481 kb on + strand, within A4249_RS05915 at 1210.482 kb on - strand, within A4249_RS05915 at 1210.482 kb on - strand, within A4249_RS05915 at 1210.482 kb on - strand, within A4249_RS05915 at 1210.482 kb on - strand, within A4249_RS05915 at 1210.482 kb on - strand, within A4249_RS05915 at 1210.482 kb on - strand, within A4249_RS05915 at 1210.482 kb on - strand, within A4249_RS05915 at 1210.598 kb on + strand, within A4249_RS05915 at 1210.599 kb on - strand, within A4249_RS05915 at 1210.648 kb on + strand, within A4249_RS05915 at 1210.649 kb on - strand, within A4249_RS05915 at 1210.700 kb on + strand, within A4249_RS05915 at 1210.701 kb on - strand, within A4249_RS05915 at 1210.701 kb on - strand, within A4249_RS05915 at 1210.815 kb on + strand at 1210.816 kb on - strand at 1210.816 kb on - strand at 1210.816 kb on - strand at 1210.816 kb on - strand at 1211.084 kb on + strand, within A4249_RS05920 at 1211.084 kb on + strand, within A4249_RS05920 at 1211.084 kb on + strand, within A4249_RS05920 at 1211.085 kb on - strand, within A4249_RS05920 at 1211.085 kb on - strand, within A4249_RS05920 at 1211.085 kb on - strand, within A4249_RS05920 at 1211.085 kb on - strand, within A4249_RS05920 at 1211.180 kb on + strand, within A4249_RS05920 at 1211.180 kb on + strand, within A4249_RS05920 at 1211.180 kb on + strand, within A4249_RS05920 at 1211.180 kb on + strand, within A4249_RS05920 at 1211.181 kb on - strand, within A4249_RS05920 at 1211.200 kb on - strand, within A4249_RS05920 at 1211.224 kb on + strand, within A4249_RS05920 at 1211.224 kb on + strand, within A4249_RS05920 at 1211.224 kb on + strand, within A4249_RS05920 at 1211.224 kb on + strand, within A4249_RS05920 at 1211.224 kb on + strand, within A4249_RS05920 at 1211.224 kb on + strand, within A4249_RS05920 at 1211.224 kb on + strand, within A4249_RS05920 at 1211.224 kb on + strand, within A4249_RS05920 at 1211.224 kb on + strand, within A4249_RS05920 at 1211.225 kb on - strand, within A4249_RS05920 at 1211.225 kb on - strand, within A4249_RS05920 at 1211.225 kb on - strand, within A4249_RS05920 at 1211.225 kb on - strand, within A4249_RS05920 at 1211.225 kb on - strand, within A4249_RS05920 at 1211.225 kb on - strand, within A4249_RS05920 at 1211.225 kb on - strand, within A4249_RS05920 at 1211.225 kb on - strand, within A4249_RS05920 at 1211.225 kb on - strand, within A4249_RS05920 at 1211.225 kb on - strand, within A4249_RS05920 at 1211.225 kb on - strand, within A4249_RS05920 at 1211.225 kb on - strand, within A4249_RS05920 at 1211.225 kb on - strand, within A4249_RS05920 at 1211.225 kb on - strand, within A4249_RS05920 at 1211.225 kb on - strand, within A4249_RS05920 at 1211.225 kb on - strand, within A4249_RS05920 at 1211.225 kb on - strand, within A4249_RS05920 at 1211.225 kb on - strand, within A4249_RS05920 at 1211.225 kb on - strand, within A4249_RS05920 at 1211.273 kb on + strand, within A4249_RS05920 at 1211.273 kb on + strand, within A4249_RS05920 at 1211.282 kb on + strand, within A4249_RS05920 at 1211.282 kb on + strand, within A4249_RS05920 at 1211.357 kb on + strand, within A4249_RS05920 at 1211.387 kb on + strand, within A4249_RS05920 at 1211.387 kb on + strand, within A4249_RS05920 at 1211.388 kb on - strand, within A4249_RS05920 at 1211.388 kb on - strand, within A4249_RS05920 at 1211.388 kb on - strand, within A4249_RS05920 at 1211.388 kb on - strand, within A4249_RS05920 at 1211.450 kb on + strand, within A4249_RS05920 at 1211.450 kb on + strand, within A4249_RS05920 at 1211.451 kb on - strand, within A4249_RS05920 at 1211.451 kb on - strand, within A4249_RS05920 at 1211.451 kb on - strand, within A4249_RS05920 at 1211.525 kb on + strand, within A4249_RS05920 at 1211.525 kb on + strand, within A4249_RS05920 at 1211.525 kb on + strand, within A4249_RS05920 at 1211.525 kb on + strand, within A4249_RS05920 at 1211.526 kb on - strand, within A4249_RS05920 at 1211.823 kb on - strand at 1211.823 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Guanosine 0.35306 mM (C) remove 1,209,912 + +0.2 1,209,912 + -0.5 1,209,912 + +0.1 1,209,913 - -0.4 1,209,913 - -0.9 1,209,913 - +0.2 1,209,913 - -1.7 1,209,913 - +0.9 1,210,037 + A4249_RS05910 0.29 -1.8 1,210,037 + A4249_RS05910 0.29 -0.1 1,210,037 + A4249_RS05910 0.29 +1.1 1,210,037 + A4249_RS05910 0.29 -0.3 1,210,037 + A4249_RS05910 0.29 -0.7 1,210,037 + A4249_RS05910 0.29 +0.7 1,210,038 - A4249_RS05910 0.29 +0.4 1,210,038 - A4249_RS05910 0.29 -0.6 1,210,038 - A4249_RS05910 0.29 -0.9 1,210,038 - A4249_RS05910 0.29 -0.1 1,210,038 - A4249_RS05910 0.29 -1.4 1,210,038 - A4249_RS05910 0.29 -0.4 1,210,038 - A4249_RS05910 0.29 -0.4 1,210,038 - A4249_RS05910 0.29 +0.3 1,210,038 - A4249_RS05910 0.29 +0.6 1,210,150 + A4249_RS05910 0.58 -0.3 1,210,150 + A4249_RS05910 0.58 -0.6 1,210,150 + A4249_RS05910 0.58 -0.3 1,210,151 - A4249_RS05910 0.59 +0.2 1,210,151 - A4249_RS05910 0.59 -0.7 1,210,179 + A4249_RS05910 0.66 -0.3 1,210,179 + A4249_RS05910 0.66 -1.1 1,210,179 + A4249_RS05910 0.66 +1.6 1,210,180 - A4249_RS05910 0.66 -0.3 1,210,229 + A4249_RS05910 0.79 -0.6 1,210,229 + A4249_RS05910 0.79 -0.1 1,210,230 - A4249_RS05910 0.80 +1.2 1,210,415 + A4249_RS05915 0.22 -0.0 1,210,415 + A4249_RS05915 0.22 +0.4 1,210,415 + A4249_RS05915 0.22 -0.9 1,210,416 - A4249_RS05915 0.22 -1.5 1,210,416 - A4249_RS05915 0.22 -1.3 1,210,416 - A4249_RS05915 0.22 -1.8 1,210,416 - A4249_RS05915 0.22 -0.1 1,210,481 + A4249_RS05915 0.35 -0.0 1,210,481 + A4249_RS05915 0.35 +0.5 1,210,481 + A4249_RS05915 0.35 +1.2 1,210,481 + A4249_RS05915 0.35 -0.7 1,210,481 + A4249_RS05915 0.35 +1.1 1,210,481 + A4249_RS05915 0.35 +0.9 1,210,481 + A4249_RS05915 0.35 -0.2 1,210,481 + A4249_RS05915 0.35 +0.6 1,210,481 + A4249_RS05915 0.35 +0.0 1,210,482 - A4249_RS05915 0.36 -0.1 1,210,482 - A4249_RS05915 0.36 +0.5 1,210,482 - A4249_RS05915 0.36 +0.6 1,210,482 - A4249_RS05915 0.36 +0.1 1,210,482 - A4249_RS05915 0.36 -0.8 1,210,482 - A4249_RS05915 0.36 -0.5 1,210,482 - A4249_RS05915 0.36 -0.3 1,210,598 + A4249_RS05915 0.59 +0.5 1,210,599 - A4249_RS05915 0.59 -1.0 1,210,648 + A4249_RS05915 0.68 +0.4 1,210,649 - A4249_RS05915 0.69 +1.4 1,210,700 + A4249_RS05915 0.79 -0.1 1,210,701 - A4249_RS05915 0.79 -0.2 1,210,701 - A4249_RS05915 0.79 +0.0 1,210,815 + -0.2 1,210,816 - -0.6 1,210,816 - +0.5 1,210,816 - +0.4 1,210,816 - +0.3 1,211,084 + A4249_RS05920 0.25 -1.1 1,211,084 + A4249_RS05920 0.25 -1.4 1,211,084 + A4249_RS05920 0.25 -0.3 1,211,085 - A4249_RS05920 0.25 +0.3 1,211,085 - A4249_RS05920 0.25 -1.2 1,211,085 - A4249_RS05920 0.25 -0.6 1,211,085 - A4249_RS05920 0.25 -0.7 1,211,180 + A4249_RS05920 0.35 +0.0 1,211,180 + A4249_RS05920 0.35 -0.4 1,211,180 + A4249_RS05920 0.35 +0.5 1,211,180 + A4249_RS05920 0.35 -0.6 1,211,181 - A4249_RS05920 0.35 +0.1 1,211,200 - A4249_RS05920 0.37 -2.5 1,211,224 + A4249_RS05920 0.40 +0.0 1,211,224 + A4249_RS05920 0.40 +0.6 1,211,224 + A4249_RS05920 0.40 -0.5 1,211,224 + A4249_RS05920 0.40 -0.4 1,211,224 + A4249_RS05920 0.40 -0.6 1,211,224 + A4249_RS05920 0.40 -0.4 1,211,224 + A4249_RS05920 0.40 -0.2 1,211,224 + A4249_RS05920 0.40 -0.6 1,211,224 + A4249_RS05920 0.40 +0.1 1,211,225 - A4249_RS05920 0.40 -0.6 1,211,225 - A4249_RS05920 0.40 -0.2 1,211,225 - A4249_RS05920 0.40 -0.2 1,211,225 - A4249_RS05920 0.40 +0.5 1,211,225 - A4249_RS05920 0.40 +0.2 1,211,225 - A4249_RS05920 0.40 +0.3 1,211,225 - A4249_RS05920 0.40 -0.2 1,211,225 - A4249_RS05920 0.40 -0.4 1,211,225 - A4249_RS05920 0.40 -1.5 1,211,225 - A4249_RS05920 0.40 -0.3 1,211,225 - A4249_RS05920 0.40 -0.1 1,211,225 - A4249_RS05920 0.40 -0.4 1,211,225 - A4249_RS05920 0.40 -0.2 1,211,225 - A4249_RS05920 0.40 +0.4 1,211,225 - A4249_RS05920 0.40 -0.1 1,211,225 - A4249_RS05920 0.40 -0.4 1,211,225 - A4249_RS05920 0.40 -0.1 1,211,225 - A4249_RS05920 0.40 +0.4 1,211,225 - A4249_RS05920 0.40 -0.4 1,211,273 + A4249_RS05920 0.45 +0.9 1,211,273 + A4249_RS05920 0.45 +0.2 1,211,282 + A4249_RS05920 0.46 -0.8 1,211,282 + A4249_RS05920 0.46 +0.2 1,211,357 + A4249_RS05920 0.54 -0.5 1,211,387 + A4249_RS05920 0.57 -0.5 1,211,387 + A4249_RS05920 0.57 +0.1 1,211,388 - A4249_RS05920 0.57 -0.8 1,211,388 - A4249_RS05920 0.57 -0.4 1,211,388 - A4249_RS05920 0.57 +0.4 1,211,388 - A4249_RS05920 0.57 -0.7 1,211,450 + A4249_RS05920 0.64 -0.5 1,211,450 + A4249_RS05920 0.64 -0.8 1,211,451 - A4249_RS05920 0.64 +0.0 1,211,451 - A4249_RS05920 0.64 -0.4 1,211,451 - A4249_RS05920 0.64 -0.3 1,211,525 + A4249_RS05920 0.71 -0.0 1,211,525 + A4249_RS05920 0.71 -0.1 1,211,525 + A4249_RS05920 0.71 -1.2 1,211,525 + A4249_RS05920 0.71 +0.4 1,211,526 - A4249_RS05920 0.72 -0.1 1,211,823 - -1.1 1,211,823 - -0.5
Or see this region's nucleotide sequence