Experiment: Guanosine 0.35306 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS01005 and A4249_RS01010 are separated by 114 nucleotides A4249_RS01010 and A4249_RS01015 are separated by 52 nucleotides A4249_RS01015 and A4249_RS01020 overlap by 1 nucleotides
A4249_RS01005: A4249_RS01005 - beta-ketoacyl-ACP synthase III, at 189,334 to 190,488
_RS01005
A4249_RS01010: A4249_RS01010 - capsular polysaccharide biosynthesis protein, at 190,603 to 192,657
_RS01010
A4249_RS01015: A4249_RS01015 - CoA ester lyase, at 192,710 to 193,546
_RS01015
A4249_RS01020: A4249_RS01020 - SDR family NAD(P)-dependent oxidoreductase, at 193,546 to 194,223
_RS01020
Position (kb) 190
191
192
193 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 189.783 kb on + strand, within A4249_RS01005 at 189.783 kb on + strand, within A4249_RS01005 at 189.784 kb on - strand, within A4249_RS01005 at 189.784 kb on - strand, within A4249_RS01005 at 189.956 kb on - strand, within A4249_RS01005 at 189.979 kb on + strand, within A4249_RS01005 at 190.027 kb on + strand, within A4249_RS01005 at 190.027 kb on + strand, within A4249_RS01005 at 190.027 kb on + strand, within A4249_RS01005 at 190.027 kb on + strand, within A4249_RS01005 at 190.027 kb on + strand, within A4249_RS01005 at 190.027 kb on + strand, within A4249_RS01005 at 190.028 kb on - strand, within A4249_RS01005 at 190.028 kb on - strand, within A4249_RS01005 at 190.028 kb on - strand, within A4249_RS01005 at 190.028 kb on - strand, within A4249_RS01005 at 190.028 kb on - strand, within A4249_RS01005 at 190.028 kb on - strand, within A4249_RS01005 at 190.094 kb on + strand, within A4249_RS01005 at 190.409 kb on + strand at 190.409 kb on + strand at 190.409 kb on + strand at 190.409 kb on + strand at 190.409 kb on + strand at 190.409 kb on + strand at 190.409 kb on + strand at 190.409 kb on + strand at 190.410 kb on - strand at 190.410 kb on - strand at 190.410 kb on - strand at 190.410 kb on - strand at 190.410 kb on - strand at 190.410 kb on - strand at 190.410 kb on - strand at 190.410 kb on - strand at 190.410 kb on - strand at 190.410 kb on - strand at 190.410 kb on - strand at 190.500 kb on + strand at 190.500 kb on + strand at 190.545 kb on - strand at 190.607 kb on + strand at 190.608 kb on - strand at 190.608 kb on - strand at 190.608 kb on - strand at 190.763 kb on + strand at 190.763 kb on + strand at 190.763 kb on + strand at 190.764 kb on - strand at 190.764 kb on - strand at 190.764 kb on - strand at 190.764 kb on - strand at 190.764 kb on - strand at 190.772 kb on + strand at 190.868 kb on + strand, within A4249_RS01010 at 190.868 kb on + strand, within A4249_RS01010 at 190.869 kb on - strand, within A4249_RS01010 at 190.869 kb on - strand, within A4249_RS01010 at 190.883 kb on + strand, within A4249_RS01010 at 190.884 kb on - strand, within A4249_RS01010 at 190.884 kb on - strand, within A4249_RS01010 at 191.073 kb on - strand, within A4249_RS01010 at 191.079 kb on + strand, within A4249_RS01010 at 191.079 kb on + strand, within A4249_RS01010 at 191.080 kb on - strand, within A4249_RS01010 at 191.080 kb on - strand, within A4249_RS01010 at 191.244 kb on + strand, within A4249_RS01010 at 191.244 kb on + strand, within A4249_RS01010 at 191.244 kb on + strand, within A4249_RS01010 at 191.244 kb on + strand, within A4249_RS01010 at 191.244 kb on + strand, within A4249_RS01010 at 191.244 kb on + strand, within A4249_RS01010 at 191.244 kb on + strand, within A4249_RS01010 at 191.244 kb on + strand, within A4249_RS01010 at 191.245 kb on - strand, within A4249_RS01010 at 191.246 kb on + strand, within A4249_RS01010 at 191.246 kb on + strand, within A4249_RS01010 at 191.246 kb on + strand, within A4249_RS01010 at 191.246 kb on + strand, within A4249_RS01010 at 191.247 kb on - strand, within A4249_RS01010 at 191.247 kb on - strand, within A4249_RS01010 at 191.247 kb on - strand, within A4249_RS01010 at 191.366 kb on + strand, within A4249_RS01010 at 191.366 kb on + strand, within A4249_RS01010 at 191.366 kb on + strand, within A4249_RS01010 at 191.367 kb on - strand, within A4249_RS01010 at 191.367 kb on - strand, within A4249_RS01010 at 191.369 kb on + strand, within A4249_RS01010 at 191.369 kb on + strand, within A4249_RS01010 at 191.369 kb on + strand, within A4249_RS01010 at 191.369 kb on + strand, within A4249_RS01010 at 191.369 kb on + strand, within A4249_RS01010 at 191.369 kb on + strand, within A4249_RS01010 at 191.370 kb on - strand, within A4249_RS01010 at 191.370 kb on - strand, within A4249_RS01010 at 191.370 kb on - strand, within A4249_RS01010 at 191.370 kb on - strand, within A4249_RS01010 at 191.582 kb on + strand, within A4249_RS01010 at 191.582 kb on + strand, within A4249_RS01010 at 191.757 kb on - strand, within A4249_RS01010 at 191.765 kb on + strand, within A4249_RS01010 at 191.861 kb on + strand, within A4249_RS01010 at 191.862 kb on - strand, within A4249_RS01010 at 191.862 kb on - strand, within A4249_RS01010 at 191.862 kb on - strand, within A4249_RS01010 at 191.936 kb on + strand, within A4249_RS01010 at 191.936 kb on + strand, within A4249_RS01010 at 191.936 kb on + strand, within A4249_RS01010 at 191.936 kb on + strand, within A4249_RS01010 at 191.936 kb on + strand, within A4249_RS01010 at 191.936 kb on + strand, within A4249_RS01010 at 191.936 kb on + strand, within A4249_RS01010 at 191.936 kb on + strand, within A4249_RS01010 at 191.936 kb on + strand, within A4249_RS01010 at 191.936 kb on + strand, within A4249_RS01010 at 191.937 kb on - strand, within A4249_RS01010 at 191.937 kb on - strand, within A4249_RS01010 at 191.937 kb on - strand, within A4249_RS01010 at 191.937 kb on - strand, within A4249_RS01010 at 191.937 kb on - strand, within A4249_RS01010 at 192.225 kb on + strand, within A4249_RS01010 at 192.225 kb on + strand, within A4249_RS01010 at 192.226 kb on - strand, within A4249_RS01010 at 192.356 kb on + strand, within A4249_RS01010 at 192.356 kb on + strand, within A4249_RS01010 at 192.356 kb on + strand, within A4249_RS01010 at 192.357 kb on - strand, within A4249_RS01010 at 192.357 kb on - strand, within A4249_RS01010 at 192.357 kb on - strand, within A4249_RS01010 at 192.357 kb on - strand, within A4249_RS01010 at 192.359 kb on + strand, within A4249_RS01010 at 192.359 kb on + strand, within A4249_RS01010 at 192.359 kb on + strand, within A4249_RS01010 at 192.360 kb on - strand, within A4249_RS01010 at 192.360 kb on - strand, within A4249_RS01010 at 192.360 kb on - strand, within A4249_RS01010 at 192.360 kb on - strand, within A4249_RS01010 at 192.360 kb on - strand, within A4249_RS01010 at 192.360 kb on - strand, within A4249_RS01010 at 192.360 kb on - strand, within A4249_RS01010 at 192.360 kb on - strand, within A4249_RS01010 at 192.360 kb on - strand, within A4249_RS01010 at 192.452 kb on + strand at 192.452 kb on + strand at 192.452 kb on + strand at 192.452 kb on + strand at 192.452 kb on + strand at 192.453 kb on - strand at 192.453 kb on - strand at 192.689 kb on - strand at 192.689 kb on - strand at 192.711 kb on + strand at 192.711 kb on + strand at 192.712 kb on - strand at 192.712 kb on - strand at 192.712 kb on - strand at 192.712 kb on - strand at 192.782 kb on + strand at 192.934 kb on - strand, within A4249_RS01015 at 192.934 kb on - strand, within A4249_RS01015 at 192.934 kb on - strand, within A4249_RS01015 at 193.272 kb on - strand, within A4249_RS01015 at 193.496 kb on + strand at 193.497 kb on - strand at 193.497 kb on - strand at 193.515 kb on + strand at 193.515 kb on + strand at 193.515 kb on + strand at 193.515 kb on + strand at 193.515 kb on + strand at 193.515 kb on + strand at 193.515 kb on + strand at 193.516 kb on - strand at 193.516 kb on - strand at 193.516 kb on - strand at 193.516 kb on - strand at 193.516 kb on - strand at 193.516 kb on - strand at 193.516 kb on - strand at 193.516 kb on - strand at 193.516 kb on - strand at 193.516 kb on - strand at 193.516 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Guanosine 0.35306 mM (C) remove 189,783 + A4249_RS01005 0.39 +2.0 189,783 + A4249_RS01005 0.39 +0.2 189,784 - A4249_RS01005 0.39 -0.1 189,784 - A4249_RS01005 0.39 +0.7 189,956 - A4249_RS01005 0.54 -0.0 189,979 + A4249_RS01005 0.56 -0.9 190,027 + A4249_RS01005 0.60 +0.9 190,027 + A4249_RS01005 0.60 +1.6 190,027 + A4249_RS01005 0.60 -0.2 190,027 + A4249_RS01005 0.60 +0.0 190,027 + A4249_RS01005 0.60 +1.3 190,027 + A4249_RS01005 0.60 -0.2 190,028 - A4249_RS01005 0.60 -1.0 190,028 - A4249_RS01005 0.60 -1.2 190,028 - A4249_RS01005 0.60 +0.6 190,028 - A4249_RS01005 0.60 -0.4 190,028 - A4249_RS01005 0.60 -0.2 190,028 - A4249_RS01005 0.60 -0.7 190,094 + A4249_RS01005 0.66 +0.5 190,409 + -0.6 190,409 + -0.3 190,409 + +0.2 190,409 + -0.0 190,409 + +0.6 190,409 + -0.2 190,409 + -0.6 190,409 + +0.2 190,410 - +0.8 190,410 - -0.1 190,410 - +1.2 190,410 - +1.7 190,410 - +0.4 190,410 - -0.1 190,410 - -0.2 190,410 - +0.1 190,410 - +1.4 190,410 - -0.2 190,410 - +0.1 190,500 + -0.8 190,500 + -0.6 190,545 - -0.2 190,607 + -0.4 190,608 - +1.7 190,608 - -0.5 190,608 - +0.2 190,763 + +1.0 190,763 + +0.4 190,763 + -0.4 190,764 - +0.6 190,764 - -0.3 190,764 - +0.5 190,764 - -1.5 190,764 - +1.4 190,772 + +0.9 190,868 + A4249_RS01010 0.13 +0.0 190,868 + A4249_RS01010 0.13 +0.0 190,869 - A4249_RS01010 0.13 +0.6 190,869 - A4249_RS01010 0.13 +0.8 190,883 + A4249_RS01010 0.14 +0.8 190,884 - A4249_RS01010 0.14 +0.8 190,884 - A4249_RS01010 0.14 -0.4 191,073 - A4249_RS01010 0.23 +1.8 191,079 + A4249_RS01010 0.23 +0.0 191,079 + A4249_RS01010 0.23 -0.2 191,080 - A4249_RS01010 0.23 +1.8 191,080 - A4249_RS01010 0.23 +0.8 191,244 + A4249_RS01010 0.31 +1.5 191,244 + A4249_RS01010 0.31 -0.5 191,244 + A4249_RS01010 0.31 +0.6 191,244 + A4249_RS01010 0.31 +0.6 191,244 + A4249_RS01010 0.31 +2.0 191,244 + A4249_RS01010 0.31 +1.0 191,244 + A4249_RS01010 0.31 -0.8 191,244 + A4249_RS01010 0.31 +1.2 191,245 - A4249_RS01010 0.31 +0.2 191,246 + A4249_RS01010 0.31 +0.5 191,246 + A4249_RS01010 0.31 +0.6 191,246 + A4249_RS01010 0.31 -0.2 191,246 + A4249_RS01010 0.31 -1.4 191,247 - A4249_RS01010 0.31 -0.4 191,247 - A4249_RS01010 0.31 +0.6 191,247 - A4249_RS01010 0.31 +0.2 191,366 + A4249_RS01010 0.37 +0.8 191,366 + A4249_RS01010 0.37 +0.0 191,366 + A4249_RS01010 0.37 -1.4 191,367 - A4249_RS01010 0.37 -0.8 191,367 - A4249_RS01010 0.37 -0.4 191,369 + A4249_RS01010 0.37 -1.0 191,369 + A4249_RS01010 0.37 -1.2 191,369 + A4249_RS01010 0.37 -0.1 191,369 + A4249_RS01010 0.37 -1.8 191,369 + A4249_RS01010 0.37 +1.0 191,369 + A4249_RS01010 0.37 -0.4 191,370 - A4249_RS01010 0.37 +0.8 191,370 - A4249_RS01010 0.37 +0.2 191,370 - A4249_RS01010 0.37 +0.2 191,370 - A4249_RS01010 0.37 +0.5 191,582 + A4249_RS01010 0.48 -0.8 191,582 + A4249_RS01010 0.48 -0.5 191,757 - A4249_RS01010 0.56 +0.5 191,765 + A4249_RS01010 0.57 +0.6 191,861 + A4249_RS01010 0.61 +1.0 191,862 - A4249_RS01010 0.61 +0.0 191,862 - A4249_RS01010 0.61 -0.9 191,862 - A4249_RS01010 0.61 +0.4 191,936 + A4249_RS01010 0.65 +0.5 191,936 + A4249_RS01010 0.65 -0.8 191,936 + A4249_RS01010 0.65 +0.0 191,936 + A4249_RS01010 0.65 +0.3 191,936 + A4249_RS01010 0.65 -1.4 191,936 + A4249_RS01010 0.65 +0.2 191,936 + A4249_RS01010 0.65 +1.0 191,936 + A4249_RS01010 0.65 +0.2 191,936 + A4249_RS01010 0.65 +0.7 191,936 + A4249_RS01010 0.65 +0.5 191,937 - A4249_RS01010 0.65 +1.0 191,937 - A4249_RS01010 0.65 -0.9 191,937 - A4249_RS01010 0.65 -1.1 191,937 - A4249_RS01010 0.65 +0.7 191,937 - A4249_RS01010 0.65 +1.4 192,225 + A4249_RS01010 0.79 -0.5 192,225 + A4249_RS01010 0.79 +1.2 192,226 - A4249_RS01010 0.79 -1.4 192,356 + A4249_RS01010 0.85 +1.8 192,356 + A4249_RS01010 0.85 +1.4 192,356 + A4249_RS01010 0.85 -0.8 192,357 - A4249_RS01010 0.85 +0.0 192,357 - A4249_RS01010 0.85 +0.8 192,357 - A4249_RS01010 0.85 -1.0 192,357 - A4249_RS01010 0.85 +2.8 192,359 + A4249_RS01010 0.85 +0.2 192,359 + A4249_RS01010 0.85 +0.5 192,359 + A4249_RS01010 0.85 +0.5 192,360 - A4249_RS01010 0.85 +2.8 192,360 - A4249_RS01010 0.85 +1.0 192,360 - A4249_RS01010 0.85 +0.0 192,360 - A4249_RS01010 0.85 -0.2 192,360 - A4249_RS01010 0.85 -1.4 192,360 - A4249_RS01010 0.85 +0.0 192,360 - A4249_RS01010 0.85 +0.8 192,360 - A4249_RS01010 0.85 -0.6 192,360 - A4249_RS01010 0.85 +1.0 192,452 + +0.9 192,452 + +1.4 192,452 + +0.4 192,452 + +1.2 192,452 + -0.4 192,453 - +0.0 192,453 - -0.6 192,689 - +1.7 192,689 - +0.4 192,711 + +0.6 192,711 + +0.7 192,712 - +1.1 192,712 - -0.5 192,712 - +0.3 192,712 - -0.2 192,782 + -0.3 192,934 - A4249_RS01015 0.27 -0.2 192,934 - A4249_RS01015 0.27 +0.1 192,934 - A4249_RS01015 0.27 -0.1 193,272 - A4249_RS01015 0.67 -0.5 193,496 + -3.3 193,497 - +0.4 193,497 - -0.1 193,515 + -0.7 193,515 + +0.2 193,515 + -0.6 193,515 + -0.2 193,515 + -0.2 193,515 + -0.2 193,515 + +0.2 193,516 - -2.4 193,516 - -1.2 193,516 - -0.3 193,516 - -0.6 193,516 - -0.4 193,516 - +0.1 193,516 - +0.8 193,516 - -2.0 193,516 - -0.6 193,516 - -3.0 193,516 - -0.4
Or see this region's nucleotide sequence