Strain Fitness in Escherichia coli ECRC102 around NIAGMN_28535

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntehxA and ehxC are separated by 1 nucleotidesehxC and tnp-IS3 are separated by 540 nucleotides NIAGMN_28530: ehxA - enterohemolysin EhxA, at 6,834 to 9,830 ehxA NIAGMN_28535: ehxC - enterohemolysin-activating lysine-acyltransferase EhxC, at 9,832 to 10,347 ehxC NIAGMN_28540: tnp-IS3 - IS3 family transposase, at 10,888 to 11,541 tnp-IS3 Position (kb) 9 10 11Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 8.858 kb on - strand, within ehxAat 8.873 kb on + strand, within ehxAat 8.888 kb on + strand, within ehxAat 8.931 kb on - strand, within ehxAat 8.931 kb on - strand, within ehxAat 9.002 kb on + strand, within ehxAat 9.003 kb on - strand, within ehxAat 9.004 kb on + strand, within ehxAat 9.005 kb on - strand, within ehxAat 9.021 kb on - strand, within ehxAat 9.021 kb on - strand, within ehxAat 9.080 kb on + strand, within ehxAat 9.080 kb on + strand, within ehxAat 9.081 kb on - strand, within ehxAat 9.081 kb on - strand, within ehxAat 9.104 kb on + strand, within ehxAat 9.117 kb on + strand, within ehxAat 9.282 kb on - strand, within ehxAat 9.293 kb on + strand, within ehxAat 9.293 kb on + strand, within ehxAat 9.294 kb on - strand, within ehxAat 9.297 kb on + strand, within ehxAat 9.321 kb on - strand, within ehxAat 9.348 kb on + strand, within ehxAat 9.462 kb on - strand, within ehxAat 9.469 kb on - strand, within ehxAat 9.495 kb on - strand, within ehxAat 9.495 kb on - strand, within ehxAat 9.618 kb on - strandat 9.618 kb on - strandat 9.640 kb on - strandat 9.681 kb on - strandat 9.889 kb on - strand, within ehxCat 10.007 kb on - strand, within ehxCat 10.039 kb on - strand, within ehxCat 10.143 kb on - strand, within ehxCat 10.252 kb on - strand, within ehxCat 10.392 kb on - strandat 10.395 kb on + strandat 10.435 kb on + strandat 10.497 kb on + strandat 10.538 kb on + strandat 10.543 kb on + strandat 10.570 kb on + strandat 10.636 kb on - strandat 10.739 kb on + strandat 10.743 kb on - strandat 10.758 kb on + strandat 10.785 kb on + strandat 10.796 kb on + strandat 10.811 kb on - strandat 10.851 kb on - strandat 10.851 kb on - strandat 10.854 kb on + strandat 10.854 kb on + strandat 10.854 kb on + strandat 10.855 kb on - strandat 10.863 kb on + strandat 10.882 kb on + strandat 10.882 kb on + strandat 10.883 kb on - strandat 10.883 kb on - strandat 10.883 kb on - strandat 10.962 kb on + strand, within tnp-IS3at 10.962 kb on + strand, within tnp-IS3at 10.964 kb on + strand, within tnp-IS3at 10.964 kb on + strand, within tnp-IS3at 10.964 kb on + strand, within tnp-IS3at 10.965 kb on - strand, within tnp-IS3at 10.965 kb on - strand, within tnp-IS3at 10.988 kb on + strand, within tnp-IS3at 10.988 kb on + strand, within tnp-IS3at 10.988 kb on + strand, within tnp-IS3at 10.989 kb on - strand, within tnp-IS3at 10.989 kb on - strand, within tnp-IS3at 11.003 kb on + strand, within tnp-IS3at 11.003 kb on + strand, within tnp-IS3at 11.004 kb on - strand, within tnp-IS3at 11.012 kb on - strand, within tnp-IS3at 11.103 kb on + strand, within tnp-IS3at 11.158 kb on + strand, within tnp-IS3at 11.158 kb on + strand, within tnp-IS3at 11.158 kb on + strand, within tnp-IS3at 11.158 kb on + strand, within tnp-IS3at 11.159 kb on - strand, within tnp-IS3at 11.159 kb on - strand, within tnp-IS3at 11.181 kb on + strand, within tnp-IS3at 11.181 kb on + strand, within tnp-IS3at 11.181 kb on + strand, within tnp-IS3at 11.181 kb on + strand, within tnp-IS3at 11.181 kb on + strand, within tnp-IS3at 11.181 kb on + strand, within tnp-IS3at 11.182 kb on - strand, within tnp-IS3at 11.182 kb on - strand, within tnp-IS3at 11.182 kb on - strand, within tnp-IS3at 11.182 kb on - strand, within tnp-IS3at 11.182 kb on - strand, within tnp-IS3at 11.182 kb on - strand, within tnp-IS3at 11.182 kb on - strand, within tnp-IS3at 11.183 kb on + strand, within tnp-IS3at 11.183 kb on + strand, within tnp-IS3at 11.184 kb on - strand, within tnp-IS3at 11.212 kb on + strand, within tnp-IS3at 11.226 kb on + strand, within tnp-IS3at 11.227 kb on - strand, within tnp-IS3at 11.342 kb on + strand, within tnp-IS3

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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8,858 - ehxA NIAGMN_28530 0.68 -1.3
8,873 + ehxA NIAGMN_28530 0.68 +0.3
8,888 + ehxA NIAGMN_28530 0.69 -0.6
8,931 - ehxA NIAGMN_28530 0.70 -1.3
8,931 - ehxA NIAGMN_28530 0.70 +0.8
9,002 + ehxA NIAGMN_28530 0.72 -0.7
9,003 - ehxA NIAGMN_28530 0.72 +1.8
9,004 + ehxA NIAGMN_28530 0.72 +2.3
9,005 - ehxA NIAGMN_28530 0.72 +0.3
9,021 - ehxA NIAGMN_28530 0.73 -1.4
9,021 - ehxA NIAGMN_28530 0.73 -0.6
9,080 + ehxA NIAGMN_28530 0.75 +0.8
9,080 + ehxA NIAGMN_28530 0.75 +4.2
9,081 - ehxA NIAGMN_28530 0.75 +2.0
9,081 - ehxA NIAGMN_28530 0.75 -0.2
9,104 + ehxA NIAGMN_28530 0.76 +1.6
9,117 + ehxA NIAGMN_28530 0.76 +0.5
9,282 - ehxA NIAGMN_28530 0.82 +1.8
9,293 + ehxA NIAGMN_28530 0.82 -0.4
9,293 + ehxA NIAGMN_28530 0.82 +1.8
9,294 - ehxA NIAGMN_28530 0.82 +2.6
9,297 + ehxA NIAGMN_28530 0.82 +3.2
9,321 - ehxA NIAGMN_28530 0.83 +3.0
9,348 + ehxA NIAGMN_28530 0.84 -0.7
9,462 - ehxA NIAGMN_28530 0.88 +0.1
9,469 - ehxA NIAGMN_28530 0.88 +1.7
9,495 - ehxA NIAGMN_28530 0.89 -0.5
9,495 - ehxA NIAGMN_28530 0.89 -1.6
9,618 - +0.0
9,618 - -1.3
9,640 - -0.0
9,681 - -0.6
9,889 - ehxC NIAGMN_28535 0.11 +2.0
10,007 - ehxC NIAGMN_28535 0.34 -0.3
10,039 - ehxC NIAGMN_28535 0.40 -1.2
10,143 - ehxC NIAGMN_28535 0.60 +0.3
10,252 - ehxC NIAGMN_28535 0.81 -1.6
10,392 - -0.9
10,395 + +0.2
10,435 + -2.4
10,497 + +3.2
10,538 + +2.5
10,543 + -0.3
10,570 + -0.9
10,636 - +0.2
10,739 + -3.0
10,743 - +0.2
10,758 + -0.3
10,785 + -2.0
10,796 + +1.6
10,811 - +1.2
10,851 - +0.4
10,851 - -0.9
10,854 + +1.4
10,854 + -2.0
10,854 + +2.9
10,855 - +2.3
10,863 + +2.4
10,882 + +2.0
10,882 + -1.2
10,883 - +0.6
10,883 - +0.5
10,883 - +0.5
10,962 + tnp-IS3 NIAGMN_28540 0.11 +1.7
10,962 + tnp-IS3 NIAGMN_28540 0.11 -0.6
10,964 + tnp-IS3 NIAGMN_28540 0.12 +0.4
10,964 + tnp-IS3 NIAGMN_28540 0.12 +1.5
10,964 + tnp-IS3 NIAGMN_28540 0.12 -1.7
10,965 - tnp-IS3 NIAGMN_28540 0.12 +0.1
10,965 - tnp-IS3 NIAGMN_28540 0.12 +1.4
10,988 + tnp-IS3 NIAGMN_28540 0.15 -1.1
10,988 + tnp-IS3 NIAGMN_28540 0.15 +0.8
10,988 + tnp-IS3 NIAGMN_28540 0.15 +1.2
10,989 - tnp-IS3 NIAGMN_28540 0.15 +0.5
10,989 - tnp-IS3 NIAGMN_28540 0.15 -1.5
11,003 + tnp-IS3 NIAGMN_28540 0.18 +0.1
11,003 + tnp-IS3 NIAGMN_28540 0.18 +0.4
11,004 - tnp-IS3 NIAGMN_28540 0.18 -1.7
11,012 - tnp-IS3 NIAGMN_28540 0.19 -0.3
11,103 + tnp-IS3 NIAGMN_28540 0.33 -0.2
11,158 + tnp-IS3 NIAGMN_28540 0.41 +0.0
11,158 + tnp-IS3 NIAGMN_28540 0.41 -0.2
11,158 + tnp-IS3 NIAGMN_28540 0.41 +1.0
11,158 + tnp-IS3 NIAGMN_28540 0.41 +2.0
11,159 - tnp-IS3 NIAGMN_28540 0.41 -1.6
11,159 - tnp-IS3 NIAGMN_28540 0.41 -0.5
11,181 + tnp-IS3 NIAGMN_28540 0.45 +0.5
11,181 + tnp-IS3 NIAGMN_28540 0.45 +0.3
11,181 + tnp-IS3 NIAGMN_28540 0.45 -0.5
11,181 + tnp-IS3 NIAGMN_28540 0.45 +0.8
11,181 + tnp-IS3 NIAGMN_28540 0.45 +0.4
11,181 + tnp-IS3 NIAGMN_28540 0.45 -0.1
11,182 - tnp-IS3 NIAGMN_28540 0.45 +0.8
11,182 - tnp-IS3 NIAGMN_28540 0.45 +1.2
11,182 - tnp-IS3 NIAGMN_28540 0.45 -1.2
11,182 - tnp-IS3 NIAGMN_28540 0.45 +0.4
11,182 - tnp-IS3 NIAGMN_28540 0.45 -0.2
11,182 - tnp-IS3 NIAGMN_28540 0.45 +1.5
11,182 - tnp-IS3 NIAGMN_28540 0.45 -0.1
11,183 + tnp-IS3 NIAGMN_28540 0.45 +1.0
11,183 + tnp-IS3 NIAGMN_28540 0.45 +1.7
11,184 - tnp-IS3 NIAGMN_28540 0.45 -1.1
11,212 + tnp-IS3 NIAGMN_28540 0.50 +1.7
11,226 + tnp-IS3 NIAGMN_28540 0.52 -0.1
11,227 - tnp-IS3 NIAGMN_28540 0.52 +0.7
11,342 + tnp-IS3 NIAGMN_28540 0.69 -2.0

Or see this region's nucleotide sequence