Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt NIAGMN_27050 and NIAGMN_27055 are separated by 278 nucleotides NIAGMN_27055 and NIAGMN_27065 are separated by 188 nucleotides NIAGMN_27065 and NIAGMN_27070 are separated by 115 nucleotides NIAGMN_27070 and NIAGMN_27075 overlap by 4 nucleotides NIAGMN_27075 and NIAGMN_27080 are separated by 35 nucleotides NIAGMN_27080 and NIAGMN_27085 are separated by 48 nucleotides
NIAGMN_27050: NIAGMN_27050 - hypothetical protein, at 5,263,706 to 5,263,873
_27050
NIAGMN_27055: NIAGMN_27055 - protein hokC, at 5,264,152 to 5,264,364
_27055
NIAGMN_27065: NIAGMN_27065 - hypothetical protein, at 5,264,553 to 5,264,657
_27065
NIAGMN_27070: NIAGMN_27070 - DUF551 domain-containing protein, at 5,264,773 to 5,265,135
_27070
NIAGMN_27075: NIAGMN_27075 - hypothetical protein, at 5,265,132 to 5,265,503
_27075
NIAGMN_27080: NIAGMN_27080 - hypothetical protein, at 5,265,539 to 5,265,751
_27080
NIAGMN_27085: NIAGMN_27085 - hypothetical protein, at 5,265,800 to 5,266,156
_27085
Position (kb) 5264
5265
5266 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 5263.828 kb on - strand, within NIAGMN_27050 at 5263.892 kb on + strand at 5263.927 kb on - strand at 5263.944 kb on - strand at 5263.944 kb on - strand at 5264.027 kb on - strand at 5264.027 kb on - strand at 5264.057 kb on - strand at 5264.364 kb on + strand at 5264.364 kb on + strand at 5264.364 kb on + strand at 5264.368 kb on + strand at 5265.758 kb on - strand at 5265.915 kb on - strand, within NIAGMN_27085
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 5,263,828 - NIAGMN_27050 0.73 -0.5 5,263,892 + +1.4 5,263,927 - -4.0 5,263,944 - +4.9 5,263,944 - -1.0 5,264,027 - +0.8 5,264,027 - -0.1 5,264,057 - -2.1 5,264,364 + +0.2 5,264,364 + -0.5 5,264,364 + -0.6 5,264,368 + +0.0 5,265,758 - +0.2 5,265,915 - NIAGMN_27085 0.32 -1.1
Or see this region's nucleotide sequence