Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt NIAGMN_27000 and NIAGMN_27005 are separated by 4 nucleotides NIAGMN_27005 and NIAGMN_27010 are separated by 76 nucleotides NIAGMN_27010 and NIAGMN_27015 are separated by 356 nucleotides NIAGMN_27015 and yhdJ are separated by 503 nucleotides
NIAGMN_27000: NIAGMN_27000 - SAR-endolysin, at 5,255,564 to 5,256,097
_27000
NIAGMN_27005: NIAGMN_27005 - holin, at 5,256,102 to 5,256,317
_27005
NIAGMN_27010: NIAGMN_27010 - DUF826 domain-containing protein, at 5,256,394 to 5,256,666
_27010
NIAGMN_27015: NIAGMN_27015 - sialate O-acetylesterase, at 5,257,023 to 5,258,969
_27015
NIAGMN_27025: yhdJ - DNA adenine methylase, at 5,259,473 to 5,260,531
yhdJ
Position (kb)
5257
5258
5259 Strain fitness (log2 ratio)
-1
0
1
2
3 at 5256.149 kb on - strand, within NIAGMN_27005 at 5256.953 kb on + strand at 5256.956 kb on + strand at 5256.956 kb on + strand at 5258.953 kb on + strand at 5258.953 kb on + strand at 5258.960 kb on + strand at 5258.994 kb on - strand at 5259.058 kb on - strand at 5259.247 kb on - strand at 5259.247 kb on - strand at 5259.776 kb on - strand, within yhdJ at 5259.776 kb on - strand, within yhdJ at 5259.778 kb on - strand, within yhdJ
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 5,256,149 - NIAGMN_27005 0.22 +0.3 5,256,953 + +0.6 5,256,956 + +0.1 5,256,956 + -1.7 5,258,953 + -0.4 5,258,953 + -0.4 5,258,960 + +0.7 5,258,994 - -0.9 5,259,058 - +1.2 5,259,247 - +1.3 5,259,247 - -1.3 5,259,776 - yhdJ NIAGMN_27025 0.29 +3.2 5,259,776 - yhdJ NIAGMN_27025 0.29 +0.4 5,259,778 - yhdJ NIAGMN_27025 0.29 -0.9
Or see this region's nucleotide sequence