Strain Fitness in Escherichia coli ECRC102 around NIAGMN_26675

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntacrA and acrR are separated by 148 nucleotidesacrR and fabI_F203V are separated by 477 nucleotides NIAGMN_26670: acrA - efflux transporter periplasmic adaptor subunit, at 5,192,453 to 5,193,574 acrA NIAGMN_26675: acrR - TetR family transcriptional regulator, at 5,193,723 to 5,194,289 acrR NIAGMN_26680: fabI_F203V - enoyl-[acyl-carrier-protein] reductase FabI, at 5,194,767 to 5,195,555 _F203V Position (kb) 5193 5194 5195Strain fitness (log2 ratio) -1 0 1 2 3 4at 5192.961 kb on + strand, within acrAat 5193.356 kb on - strand, within acrAat 5193.534 kb on - strandat 5193.567 kb on + strandat 5193.760 kb on - strandat 5194.145 kb on - strand, within acrRat 5194.191 kb on - strand, within acrRat 5194.382 kb on + strandat 5194.445 kb on - strandat 5194.447 kb on + strandat 5194.559 kb on + strandat 5194.594 kb on + strandat 5194.649 kb on + strandat 5194.674 kb on + strandat 5194.697 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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5,192,961 + acrA NIAGMN_26670 0.45 -0.4
5,193,356 - acrA NIAGMN_26670 0.80 +1.3
5,193,534 - -0.3
5,193,567 + -1.4
5,193,760 - -0.9
5,194,145 - acrR NIAGMN_26675 0.74 +0.8
5,194,191 - acrR NIAGMN_26675 0.83 -0.4
5,194,382 + +0.8
5,194,445 - +1.0
5,194,447 + +0.3
5,194,559 + +0.1
5,194,594 + +1.7
5,194,649 + -0.7
5,194,674 + +1.4
5,194,697 + +3.8

Or see this region's nucleotide sequence