Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lolE and NIAGMN_23925 are separated by 8 nucleotides NIAGMN_23925 and NIAGMN_23930 overlap by 4 nucleotides NIAGMN_23930 and ymdE are separated by 13 nucleotides ymdE and ycdU overlap by 4 nucleotides
NIAGMN_23920: lolE - ABC transporter permease, at 4,684,824 to 4,686,134
lolE
NIAGMN_23925: NIAGMN_23925 - Outer membrane lipoprotein-sorting protein, at 4,686,143 to 4,686,931
_23925
NIAGMN_23930: NIAGMN_23930 - hypothetical protein, at 4,686,928 to 4,688,316
_23930
NIAGMN_23935: ymdE - Putative uncharacterized protein YmdE, at 4,688,330 to 4,689,271
ymdE
NIAGMN_23940: ycdU - Uncharacterized protein YcdU, at 4,689,268 to 4,690,254
ycdU
Position (kb) 4686
4687
4688
4689 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 4685.994 kb on - strand, within lolE at 4686.579 kb on - strand, within NIAGMN_23925 at 4686.748 kb on - strand, within NIAGMN_23925 at 4686.910 kb on + strand at 4686.911 kb on - strand at 4688.199 kb on - strand at 4688.219 kb on - strand at 4688.290 kb on + strand at 4688.312 kb on + strand at 4688.312 kb on + strand at 4688.313 kb on - strand at 4688.313 kb on - strand at 4688.313 kb on - strand at 4688.813 kb on - strand, within ymdE at 4689.134 kb on - strand, within ymdE at 4689.167 kb on - strand, within ymdE at 4689.227 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 4,685,994 - lolE NIAGMN_23920 0.89 +0.8 4,686,579 - NIAGMN_23925 0.55 +1.3 4,686,748 - NIAGMN_23925 0.77 +2.9 4,686,910 + -2.9 4,686,911 - +2.4 4,688,199 - -0.7 4,688,219 - -0.1 4,688,290 + -1.2 4,688,312 + +1.6 4,688,312 + -1.2 4,688,313 - -1.1 4,688,313 - +1.5 4,688,313 - -2.0 4,688,813 - ymdE NIAGMN_23935 0.51 -0.8 4,689,134 - ymdE NIAGMN_23935 0.85 +0.3 4,689,167 - ymdE NIAGMN_23935 0.89 +1.1 4,689,227 - -0.4
Or see this region's nucleotide sequence