Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt stxA2c and stxB2a are separated by 11 nucleotides stxB2a and NIAGMN_23460 are separated by 486 nucleotides NIAGMN_23460 and NIAGMN_23465 are separated by 134 nucleotides NIAGMN_23465 and NIAGMN_23470 are separated by 40 nucleotides NIAGMN_23470 and NIAGMN_23475 are separated by 76 nucleotides NIAGMN_23475 and NIAGMN_23480 are separated by 4 nucleotides
NIAGMN_23440: stxA2c - Shiga toxin Stx2c subunit A, at 4,588,270 to 4,589,229
stxA2c
NIAGMN_23445: stxB2a - Shiga toxin Stx2a subunit B, at 4,589,241 to 4,589,510
stxB2a
NIAGMN_23460: NIAGMN_23460 - sialate O-acetylesterase, at 4,589,997 to 4,591,934
_23460
NIAGMN_23465: NIAGMN_23465 - DUF1378 domain-containing protein, at 4,592,069 to 4,592,248
_23465
NIAGMN_23470: NIAGMN_23470 - DUF826 domain-containing protein, at 4,592,289 to 4,592,561
_23470
NIAGMN_23475: NIAGMN_23475 - holin, at 4,592,638 to 4,592,853
_23475
NIAGMN_23480: NIAGMN_23480 - SAR-endolysin, at 4,592,858 to 4,593,391
_23480
Position (kb)
4589
4590
4591
4592 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4589.154 kb on + strand at 4589.154 kb on + strand at 4589.210 kb on + strand at 4589.215 kb on - strand at 4589.336 kb on + strand, within stxB2a at 4589.498 kb on + strand at 4589.501 kb on + strand at 4589.501 kb on + strand at 4589.531 kb on - strand at 4589.548 kb on - strand at 4589.735 kb on + strand at 4589.752 kb on + strand at 4589.752 kb on + strand at 4589.754 kb on + strand at 4589.754 kb on + strand at 4589.754 kb on + strand at 4589.755 kb on - strand at 4589.755 kb on - strand at 4589.785 kb on - strand at 4589.791 kb on - strand at 4589.791 kb on - strand at 4589.827 kb on + strand at 4589.828 kb on - strand at 4589.837 kb on - strand at 4589.867 kb on + strand at 4589.910 kb on - strand at 4589.975 kb on - strand at 4590.231 kb on + strand, within NIAGMN_23460 at 4590.270 kb on + strand, within NIAGMN_23460 at 4590.270 kb on + strand, within NIAGMN_23460 at 4590.320 kb on + strand, within NIAGMN_23460 at 4590.344 kb on + strand, within NIAGMN_23460 at 4590.344 kb on + strand, within NIAGMN_23460 at 4590.918 kb on + strand, within NIAGMN_23460 at 4591.888 kb on + strand at 4592.014 kb on + strand at 4592.043 kb on - strand at 4592.444 kb on - strand, within NIAGMN_23470
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 4,589,154 + -1.2 4,589,154 + -1.3 4,589,210 + +1.9 4,589,215 - -2.1 4,589,336 + stxB2a NIAGMN_23445 0.35 -2.4 4,589,498 + +0.8 4,589,501 + -2.9 4,589,501 + +0.4 4,589,531 - -0.1 4,589,548 - -3.5 4,589,735 + -3.1 4,589,752 + +0.2 4,589,752 + +1.6 4,589,754 + -1.8 4,589,754 + -2.1 4,589,754 + +1.9 4,589,755 - -0.4 4,589,755 - +0.4 4,589,785 - -1.0 4,589,791 - -1.0 4,589,791 - -2.3 4,589,827 + -1.5 4,589,828 - -0.3 4,589,837 - +0.5 4,589,867 + -2.2 4,589,910 - -1.2 4,589,975 - -0.2 4,590,231 + NIAGMN_23460 0.12 +0.0 4,590,270 + NIAGMN_23460 0.14 -0.6 4,590,270 + NIAGMN_23460 0.14 +1.0 4,590,320 + NIAGMN_23460 0.17 +1.8 4,590,344 + NIAGMN_23460 0.18 +0.1 4,590,344 + NIAGMN_23460 0.18 -2.1 4,590,918 + NIAGMN_23460 0.48 -2.6 4,591,888 + -1.6 4,592,014 + +0.8 4,592,043 - -3.4 4,592,444 - NIAGMN_23470 0.57 -1.2
Or see this region's nucleotide sequence