Strain Fitness in Escherichia coli ECRC102 around NIAGMN_20535

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyfcH and folX are separated by 20 nucleotidesfolX and yfcG are separated by 56 nucleotidesyfcG and yfcF are separated by 135 nucleotidesyfcF and yfcE are separated by 55 nucleotides NIAGMN_20525: yfcH - Epimerase family protein YfcH, at 4,052,900 to 4,053,793 yfcH NIAGMN_20530: folX - dihydroneopterin triphosphate 2'-epimerase, at 4,053,814 to 4,054,176 folX NIAGMN_20535: yfcG - GSH-dependent disulfide bond oxidoreductase, at 4,054,233 to 4,054,880 yfcG NIAGMN_20540: yfcF - glutathione transferase, at 4,055,016 to 4,055,660 yfcF NIAGMN_20545: yfcE - phosphodiesterase, at 4,055,716 to 4,056,267 yfcE Position (kb) 4054 4055Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 4053.432 kb on - strand, within yfcHat 4053.432 kb on - strand, within yfcHat 4053.432 kb on - strand, within yfcHat 4053.445 kb on - strand, within yfcHat 4053.445 kb on - strand, within yfcHat 4053.445 kb on - strand, within yfcHat 4053.761 kb on + strandat 4053.786 kb on - strandat 4053.786 kb on - strandat 4053.893 kb on + strand, within folXat 4053.894 kb on - strand, within folXat 4053.894 kb on - strand, within folXat 4053.894 kb on - strand, within folXat 4053.894 kb on - strand, within folXat 4053.966 kb on + strand, within folXat 4053.967 kb on - strand, within folXat 4053.985 kb on - strand, within folXat 4054.027 kb on - strand, within folXat 4054.113 kb on + strand, within folXat 4054.129 kb on - strand, within folXat 4054.147 kb on + strandat 4054.148 kb on - strandat 4054.156 kb on - strandat 4054.156 kb on - strandat 4054.197 kb on - strandat 4054.201 kb on - strandat 4054.210 kb on + strandat 4054.269 kb on + strandat 4054.333 kb on + strand, within yfcGat 4054.338 kb on - strand, within yfcGat 4054.338 kb on - strand, within yfcGat 4054.347 kb on + strand, within yfcGat 4054.453 kb on - strand, within yfcGat 4054.484 kb on - strand, within yfcGat 4054.649 kb on - strand, within yfcGat 4054.789 kb on - strand, within yfcGat 4054.918 kb on + strandat 4054.918 kb on + strandat 4054.918 kb on + strandat 4054.918 kb on + strandat 4054.937 kb on + strandat 4054.937 kb on + strandat 4054.938 kb on - strandat 4054.938 kb on - strandat 4054.945 kb on + strandat 4054.945 kb on + strandat 4054.976 kb on + strandat 4054.978 kb on + strandat 4054.978 kb on + strandat 4054.979 kb on - strandat 4054.979 kb on - strandat 4055.001 kb on - strandat 4055.015 kb on - strandat 4055.015 kb on - strandat 4055.015 kb on - strandat 4055.067 kb on + strandat 4055.067 kb on + strandat 4055.067 kb on + strandat 4055.067 kb on + strandat 4055.067 kb on + strandat 4055.172 kb on + strand, within yfcFat 4055.247 kb on + strand, within yfcFat 4055.285 kb on + strand, within yfcFat 4055.285 kb on + strand, within yfcFat 4055.286 kb on - strand, within yfcFat 4055.286 kb on - strand, within yfcFat 4055.286 kb on - strand, within yfcFat 4055.302 kb on + strand, within yfcFat 4055.416 kb on + strand, within yfcFat 4055.551 kb on + strand, within yfcFat 4055.554 kb on + strand, within yfcFat 4055.554 kb on + strand, within yfcFat 4055.554 kb on + strand, within yfcFat 4055.554 kb on + strand, within yfcFat 4055.685 kb on + strandat 4055.685 kb on + strandat 4055.685 kb on + strandat 4055.685 kb on + strandat 4055.685 kb on + strandat 4055.685 kb on + strandat 4055.685 kb on + strandat 4055.685 kb on + strandat 4055.685 kb on + strandat 4055.685 kb on + strandat 4055.685 kb on + strandat 4055.685 kb on + strandat 4055.686 kb on - strandat 4055.686 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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4,053,432 - yfcH NIAGMN_20525 0.60 -0.6
4,053,432 - yfcH NIAGMN_20525 0.60 -1.8
4,053,432 - yfcH NIAGMN_20525 0.60 +1.6
4,053,445 - yfcH NIAGMN_20525 0.61 -0.3
4,053,445 - yfcH NIAGMN_20525 0.61 +0.1
4,053,445 - yfcH NIAGMN_20525 0.61 -0.9
4,053,761 + +1.6
4,053,786 - +0.7
4,053,786 - -1.0
4,053,893 + folX NIAGMN_20530 0.22 -2.3
4,053,894 - folX NIAGMN_20530 0.22 +0.1
4,053,894 - folX NIAGMN_20530 0.22 +4.1
4,053,894 - folX NIAGMN_20530 0.22 -0.3
4,053,894 - folX NIAGMN_20530 0.22 +0.8
4,053,966 + folX NIAGMN_20530 0.42 +2.3
4,053,967 - folX NIAGMN_20530 0.42 -1.1
4,053,985 - folX NIAGMN_20530 0.47 -1.9
4,054,027 - folX NIAGMN_20530 0.59 -1.1
4,054,113 + folX NIAGMN_20530 0.82 -1.1
4,054,129 - folX NIAGMN_20530 0.87 +1.0
4,054,147 + +0.3
4,054,148 - -2.0
4,054,156 - -0.8
4,054,156 - +2.6
4,054,197 - -0.4
4,054,201 - -0.4
4,054,210 + -0.7
4,054,269 + +1.5
4,054,333 + yfcG NIAGMN_20535 0.15 +1.0
4,054,338 - yfcG NIAGMN_20535 0.16 -0.7
4,054,338 - yfcG NIAGMN_20535 0.16 +0.1
4,054,347 + yfcG NIAGMN_20535 0.18 +0.3
4,054,453 - yfcG NIAGMN_20535 0.34 +1.3
4,054,484 - yfcG NIAGMN_20535 0.39 +1.7
4,054,649 - yfcG NIAGMN_20535 0.64 +2.0
4,054,789 - yfcG NIAGMN_20535 0.86 -2.5
4,054,918 + +1.8
4,054,918 + -1.3
4,054,918 + +1.5
4,054,918 + -0.4
4,054,937 + -0.2
4,054,937 + +2.1
4,054,938 - -0.3
4,054,938 - +0.1
4,054,945 + -0.3
4,054,945 + +1.0
4,054,976 + +0.1
4,054,978 + +3.4
4,054,978 + -0.2
4,054,979 - +0.2
4,054,979 - +0.1
4,055,001 - -0.4
4,055,015 - +2.9
4,055,015 - +1.7
4,055,015 - +0.2
4,055,067 + -0.0
4,055,067 + +0.1
4,055,067 + -2.0
4,055,067 + +0.5
4,055,067 + +0.9
4,055,172 + yfcF NIAGMN_20540 0.24 +0.1
4,055,247 + yfcF NIAGMN_20540 0.36 +0.1
4,055,285 + yfcF NIAGMN_20540 0.42 +0.2
4,055,285 + yfcF NIAGMN_20540 0.42 -0.2
4,055,286 - yfcF NIAGMN_20540 0.42 -0.7
4,055,286 - yfcF NIAGMN_20540 0.42 -1.3
4,055,286 - yfcF NIAGMN_20540 0.42 +1.8
4,055,302 + yfcF NIAGMN_20540 0.44 +1.1
4,055,416 + yfcF NIAGMN_20540 0.62 +0.5
4,055,551 + yfcF NIAGMN_20540 0.83 -0.5
4,055,554 + yfcF NIAGMN_20540 0.83 +0.4
4,055,554 + yfcF NIAGMN_20540 0.83 -0.9
4,055,554 + yfcF NIAGMN_20540 0.83 -1.2
4,055,554 + yfcF NIAGMN_20540 0.83 -0.3
4,055,685 + +0.0
4,055,685 + +1.7
4,055,685 + +2.5
4,055,685 + +0.0
4,055,685 + -0.3
4,055,685 + +0.0
4,055,685 + -1.0
4,055,685 + +0.7
4,055,685 + -0.5
4,055,685 + +0.4
4,055,685 + +1.3
4,055,685 + +0.3
4,055,686 - +1.7
4,055,686 - +0.8

Or see this region's nucleotide sequence