Strain Fitness in Escherichia coli ECRC102 around NIAGMN_19195

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntera and recO are separated by 11 nucleotidesrecO and pdxJ are separated by 11 nucleotidespdxJ and acpS overlap by 1 nucleotidesacpS and shoB are separated by 421 nucleotides NIAGMN_19185: era - GTPase Era, at 3,777,901 to 3,778,806 era NIAGMN_19190: recO - DNA repair protein RecO, at 3,778,818 to 3,779,546 recO NIAGMN_19195: pdxJ - pyridoxine 5'-phosphate synthase, at 3,779,558 to 3,780,289 pdxJ NIAGMN_19200: acpS - Holo-[acyl-carrier-protein] synthase, at 3,780,289 to 3,780,669 acpS NIAGMN_19210: shoB - Small toxic protein ShoB, at 3,781,091 to 3,781,171 shoB Position (kb) 3779 3780 3781Strain fitness (log2 ratio) -2 -1 0 1at 3778.789 kb on + strandat 3778.966 kb on + strand, within recOat 3778.966 kb on + strand, within recOat 3778.967 kb on - strand, within recOat 3779.121 kb on + strand, within recOat 3779.154 kb on + strand, within recOat 3779.155 kb on - strand, within recOat 3779.357 kb on + strand, within recOat 3779.456 kb on + strand, within recOat 3779.463 kb on + strand, within recOat 3779.510 kb on + strandat 3779.510 kb on + strandat 3779.568 kb on + strandat 3779.694 kb on + strand, within pdxJat 3780.032 kb on + strand, within pdxJat 3780.156 kb on + strand, within pdxJat 3780.691 kb on - strandat 3781.045 kb on + strandat 3781.045 kb on + strandat 3781.045 kb on + strandat 3781.045 kb on + strandat 3781.046 kb on - strandat 3781.170 kb on - strandat 3781.233 kb on - strandat 3781.237 kb on - strandat 3781.256 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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3,778,789 + +1.3
3,778,966 + recO NIAGMN_19190 0.20 -2.2
3,778,966 + recO NIAGMN_19190 0.20 -2.4
3,778,967 - recO NIAGMN_19190 0.20 -2.1
3,779,121 + recO NIAGMN_19190 0.42 -0.7
3,779,154 + recO NIAGMN_19190 0.46 -2.5
3,779,155 - recO NIAGMN_19190 0.46 +0.6
3,779,357 + recO NIAGMN_19190 0.74 -1.6
3,779,456 + recO NIAGMN_19190 0.88 -2.2
3,779,463 + recO NIAGMN_19190 0.88 -0.9
3,779,510 + +0.5
3,779,510 + +0.1
3,779,568 + -0.6
3,779,694 + pdxJ NIAGMN_19195 0.19 +0.0
3,780,032 + pdxJ NIAGMN_19195 0.65 -2.6
3,780,156 + pdxJ NIAGMN_19195 0.82 -2.0
3,780,691 - +0.4
3,781,045 + +0.6
3,781,045 + -0.4
3,781,045 + -0.6
3,781,045 + +0.5
3,781,046 - +0.3
3,781,170 - -0.1
3,781,233 - +0.9
3,781,237 - +1.3
3,781,256 - -1.8

Or see this region's nucleotide sequence