Strain Fitness in Escherichia coli ECRC102 around NIAGMN_14415

Experiment: Sodium-DL-Lactate

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntNIAGMN_14410 and NIAGMN_14415 are separated by 68 nucleotidesNIAGMN_14415 and yhhT are separated by 391 nucleotides NIAGMN_14410: NIAGMN_14410 - SAM-dependent methyltransferase, at 2,867,672 to 2,868,730 _14410 NIAGMN_14415: NIAGMN_14415 - hypothetical protein, at 2,868,799 to 2,869,176 _14415 NIAGMN_14420: yhhT - Putative transport protein YhhT, at 2,869,568 to 2,870,617 yhhT Position (kb) 2868 2869 2870Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2867.812 kb on + strand, within NIAGMN_14410at 2867.813 kb on - strand, within NIAGMN_14410at 2867.817 kb on + strand, within NIAGMN_14410at 2867.817 kb on + strand, within NIAGMN_14410at 2867.912 kb on + strand, within NIAGMN_14410at 2867.912 kb on + strand, within NIAGMN_14410at 2867.934 kb on - strand, within NIAGMN_14410at 2867.938 kb on + strand, within NIAGMN_14410at 2867.939 kb on - strand, within NIAGMN_14410at 2867.939 kb on - strand, within NIAGMN_14410at 2867.942 kb on + strand, within NIAGMN_14410at 2867.942 kb on + strand, within NIAGMN_14410at 2867.943 kb on - strand, within NIAGMN_14410at 2867.990 kb on - strand, within NIAGMN_14410at 2868.020 kb on + strand, within NIAGMN_14410at 2868.020 kb on + strand, within NIAGMN_14410at 2868.021 kb on - strand, within NIAGMN_14410at 2868.044 kb on + strand, within NIAGMN_14410at 2868.087 kb on - strand, within NIAGMN_14410at 2868.087 kb on - strand, within NIAGMN_14410at 2868.087 kb on - strand, within NIAGMN_14410at 2868.110 kb on + strand, within NIAGMN_14410at 2868.110 kb on + strand, within NIAGMN_14410at 2868.111 kb on - strand, within NIAGMN_14410at 2868.111 kb on - strand, within NIAGMN_14410at 2868.111 kb on - strand, within NIAGMN_14410at 2868.111 kb on - strand, within NIAGMN_14410at 2868.111 kb on - strand, within NIAGMN_14410at 2868.111 kb on - strand, within NIAGMN_14410at 2868.111 kb on - strand, within NIAGMN_14410at 2868.118 kb on + strand, within NIAGMN_14410at 2868.118 kb on + strand, within NIAGMN_14410at 2868.119 kb on - strand, within NIAGMN_14410at 2868.164 kb on - strand, within NIAGMN_14410at 2868.180 kb on - strand, within NIAGMN_14410at 2868.180 kb on - strand, within NIAGMN_14410at 2868.186 kb on + strand, within NIAGMN_14410at 2868.186 kb on + strand, within NIAGMN_14410at 2868.186 kb on + strand, within NIAGMN_14410at 2868.186 kb on + strand, within NIAGMN_14410at 2868.186 kb on + strand, within NIAGMN_14410at 2868.187 kb on - strand, within NIAGMN_14410at 2868.187 kb on - strand, within NIAGMN_14410at 2868.212 kb on + strand, within NIAGMN_14410at 2868.212 kb on + strand, within NIAGMN_14410at 2868.212 kb on + strand, within NIAGMN_14410at 2868.212 kb on + strand, within NIAGMN_14410at 2868.212 kb on + strand, within NIAGMN_14410at 2868.213 kb on - strand, within NIAGMN_14410at 2868.213 kb on - strand, within NIAGMN_14410at 2868.216 kb on + strand, within NIAGMN_14410at 2868.216 kb on + strand, within NIAGMN_14410at 2868.216 kb on + strand, within NIAGMN_14410at 2868.216 kb on + strand, within NIAGMN_14410at 2868.216 kb on + strand, within NIAGMN_14410at 2868.217 kb on - strand, within NIAGMN_14410at 2868.232 kb on + strand, within NIAGMN_14410at 2868.249 kb on + strand, within NIAGMN_14410at 2868.249 kb on + strand, within NIAGMN_14410at 2868.250 kb on - strand, within NIAGMN_14410at 2868.252 kb on + strand, within NIAGMN_14410at 2868.252 kb on + strand, within NIAGMN_14410at 2868.252 kb on + strand, within NIAGMN_14410at 2868.253 kb on - strand, within NIAGMN_14410at 2868.293 kb on + strand, within NIAGMN_14410at 2868.313 kb on - strand, within NIAGMN_14410at 2868.314 kb on + strand, within NIAGMN_14410at 2868.315 kb on - strand, within NIAGMN_14410at 2868.315 kb on - strand, within NIAGMN_14410at 2868.315 kb on - strand, within NIAGMN_14410at 2868.315 kb on - strand, within NIAGMN_14410at 2868.335 kb on + strand, within NIAGMN_14410at 2868.336 kb on - strand, within NIAGMN_14410at 2868.342 kb on - strand, within NIAGMN_14410at 2868.397 kb on - strand, within NIAGMN_14410at 2868.436 kb on + strand, within NIAGMN_14410at 2868.436 kb on + strand, within NIAGMN_14410at 2868.437 kb on - strand, within NIAGMN_14410at 2868.437 kb on - strand, within NIAGMN_14410at 2868.453 kb on + strand, within NIAGMN_14410at 2868.454 kb on - strand, within NIAGMN_14410at 2868.454 kb on - strand, within NIAGMN_14410at 2868.454 kb on - strand, within NIAGMN_14410at 2868.474 kb on + strand, within NIAGMN_14410at 2868.474 kb on + strand, within NIAGMN_14410at 2868.475 kb on - strand, within NIAGMN_14410at 2868.478 kb on - strand, within NIAGMN_14410at 2868.490 kb on + strand, within NIAGMN_14410at 2868.496 kb on + strand, within NIAGMN_14410at 2868.499 kb on + strand, within NIAGMN_14410at 2868.500 kb on - strand, within NIAGMN_14410at 2868.500 kb on - strand, within NIAGMN_14410at 2868.500 kb on - strand, within NIAGMN_14410at 2868.500 kb on - strand, within NIAGMN_14410at 2868.500 kb on - strand, within NIAGMN_14410at 2868.500 kb on - strand, within NIAGMN_14410at 2868.520 kb on + strand, within NIAGMN_14410at 2868.521 kb on - strand, within NIAGMN_14410at 2868.538 kb on + strand, within NIAGMN_14410at 2868.543 kb on + strand, within NIAGMN_14410at 2868.543 kb on + strand, within NIAGMN_14410at 2868.543 kb on + strand, within NIAGMN_14410at 2868.543 kb on + strand, within NIAGMN_14410at 2868.544 kb on - strand, within NIAGMN_14410at 2868.553 kb on + strand, within NIAGMN_14410at 2868.554 kb on - strand, within NIAGMN_14410at 2868.559 kb on + strand, within NIAGMN_14410at 2868.559 kb on + strand, within NIAGMN_14410at 2868.559 kb on + strand, within NIAGMN_14410at 2868.560 kb on - strand, within NIAGMN_14410at 2868.560 kb on - strand, within NIAGMN_14410at 2868.560 kb on - strand, within NIAGMN_14410at 2868.613 kb on - strand, within NIAGMN_14410at 2868.620 kb on + strand, within NIAGMN_14410at 2868.621 kb on - strand, within NIAGMN_14410at 2868.621 kb on - strand, within NIAGMN_14410at 2868.621 kb on - strand, within NIAGMN_14410at 2868.632 kb on - strandat 2868.632 kb on - strandat 2868.635 kb on + strandat 2868.635 kb on + strandat 2868.636 kb on - strandat 2868.636 kb on - strandat 2868.709 kb on + strandat 2868.726 kb on + strandat 2868.726 kb on + strandat 2868.726 kb on + strandat 2868.726 kb on + strandat 2868.726 kb on + strandat 2868.726 kb on + strandat 2868.726 kb on + strandat 2868.727 kb on - strandat 2868.727 kb on - strandat 2868.748 kb on - strandat 2868.764 kb on - strandat 2868.801 kb on - strandat 2868.899 kb on - strand, within NIAGMN_14415at 2868.944 kb on + strand, within NIAGMN_14415at 2868.944 kb on + strand, within NIAGMN_14415at 2868.944 kb on + strand, within NIAGMN_14415at 2868.944 kb on + strand, within NIAGMN_14415at 2869.077 kb on + strand, within NIAGMN_14415at 2869.138 kb on + strand, within NIAGMN_14415at 2869.178 kb on + strandat 2869.179 kb on - strandat 2869.179 kb on - strandat 2869.179 kb on - strandat 2869.179 kb on - strandat 2869.189 kb on + strandat 2869.190 kb on - strandat 2869.238 kb on + strandat 2869.255 kb on - strandat 2869.266 kb on - strandat 2869.266 kb on - strandat 2869.278 kb on - strandat 2869.288 kb on + strandat 2869.331 kb on + strandat 2869.332 kb on - strandat 2869.358 kb on + strandat 2869.359 kb on - strandat 2869.445 kb on - strandat 2869.445 kb on - strandat 2869.446 kb on + strandat 2869.447 kb on - strandat 2869.448 kb on + strandat 2869.530 kb on + strandat 2869.531 kb on - strandat 2869.531 kb on - strandat 2869.566 kb on + strandat 2869.566 kb on + strandat 2869.566 kb on + strandat 2869.566 kb on + strandat 2869.566 kb on + strandat 2869.567 kb on - strandat 2869.567 kb on - strandat 2869.567 kb on - strandat 2869.567 kb on - strandat 2869.607 kb on + strandat 2869.682 kb on + strand, within yhhTat 2869.682 kb on + strand, within yhhTat 2869.682 kb on + strand, within yhhTat 2869.682 kb on + strand, within yhhTat 2869.718 kb on + strand, within yhhTat 2869.718 kb on + strand, within yhhTat 2869.733 kb on + strand, within yhhTat 2869.733 kb on + strand, within yhhTat 2869.734 kb on - strand, within yhhTat 2869.734 kb on - strand, within yhhTat 2869.734 kb on - strand, within yhhTat 2869.734 kb on - strand, within yhhTat 2869.801 kb on + strand, within yhhTat 2869.801 kb on + strand, within yhhTat 2869.834 kb on + strand, within yhhTat 2869.834 kb on + strand, within yhhTat 2869.834 kb on + strand, within yhhTat 2869.835 kb on - strand, within yhhTat 2869.835 kb on - strand, within yhhTat 2869.835 kb on - strand, within yhhTat 2869.914 kb on + strand, within yhhTat 2869.914 kb on + strand, within yhhTat 2869.914 kb on + strand, within yhhTat 2869.915 kb on - strand, within yhhTat 2869.915 kb on - strand, within yhhTat 2869.915 kb on - strand, within yhhTat 2869.915 kb on - strand, within yhhTat 2869.915 kb on - strand, within yhhTat 2869.915 kb on - strand, within yhhTat 2869.915 kb on - strand, within yhhTat 2869.915 kb on - strand, within yhhTat 2869.915 kb on - strand, within yhhTat 2869.934 kb on + strand, within yhhTat 2869.934 kb on + strand, within yhhTat 2869.959 kb on - strand, within yhhTat 2870.003 kb on + strand, within yhhTat 2870.047 kb on + strand, within yhhTat 2870.048 kb on - strand, within yhhTat 2870.048 kb on - strand, within yhhTat 2870.048 kb on - strand, within yhhTat 2870.109 kb on + strand, within yhhTat 2870.137 kb on + strand, within yhhTat 2870.144 kb on + strand, within yhhTat 2870.145 kb on - strand, within yhhT

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
remove
2,867,812 + NIAGMN_14410 0.13 -1.2
2,867,813 - NIAGMN_14410 0.13 -0.3
2,867,817 + NIAGMN_14410 0.14 +2.3
2,867,817 + NIAGMN_14410 0.14 -2.9
2,867,912 + NIAGMN_14410 0.23 -1.1
2,867,912 + NIAGMN_14410 0.23 -0.4
2,867,934 - NIAGMN_14410 0.25 -0.1
2,867,938 + NIAGMN_14410 0.25 -1.9
2,867,939 - NIAGMN_14410 0.25 -1.6
2,867,939 - NIAGMN_14410 0.25 +0.5
2,867,942 + NIAGMN_14410 0.25 -0.2
2,867,942 + NIAGMN_14410 0.25 -0.0
2,867,943 - NIAGMN_14410 0.26 -0.8
2,867,990 - NIAGMN_14410 0.30 -0.6
2,868,020 + NIAGMN_14410 0.33 -0.0
2,868,020 + NIAGMN_14410 0.33 +1.0
2,868,021 - NIAGMN_14410 0.33 +2.4
2,868,044 + NIAGMN_14410 0.35 -2.7
2,868,087 - NIAGMN_14410 0.39 -1.2
2,868,087 - NIAGMN_14410 0.39 -0.1
2,868,087 - NIAGMN_14410 0.39 -1.1
2,868,110 + NIAGMN_14410 0.41 +0.4
2,868,110 + NIAGMN_14410 0.41 -0.4
2,868,111 - NIAGMN_14410 0.41 +0.1
2,868,111 - NIAGMN_14410 0.41 +0.2
2,868,111 - NIAGMN_14410 0.41 -0.3
2,868,111 - NIAGMN_14410 0.41 -0.1
2,868,111 - NIAGMN_14410 0.41 -0.9
2,868,111 - NIAGMN_14410 0.41 +0.4
2,868,111 - NIAGMN_14410 0.41 -0.7
2,868,118 + NIAGMN_14410 0.42 +0.2
2,868,118 + NIAGMN_14410 0.42 +0.2
2,868,119 - NIAGMN_14410 0.42 -0.7
2,868,164 - NIAGMN_14410 0.46 -0.2
2,868,180 - NIAGMN_14410 0.48 +0.3
2,868,180 - NIAGMN_14410 0.48 -0.2
2,868,186 + NIAGMN_14410 0.49 +1.3
2,868,186 + NIAGMN_14410 0.49 +2.8
2,868,186 + NIAGMN_14410 0.49 +0.5
2,868,186 + NIAGMN_14410 0.49 +3.3
2,868,186 + NIAGMN_14410 0.49 -0.6
2,868,187 - NIAGMN_14410 0.49 -0.4
2,868,187 - NIAGMN_14410 0.49 +1.4
2,868,212 + NIAGMN_14410 0.51 -0.8
2,868,212 + NIAGMN_14410 0.51 +0.2
2,868,212 + NIAGMN_14410 0.51 +0.6
2,868,212 + NIAGMN_14410 0.51 +1.4
2,868,212 + NIAGMN_14410 0.51 -0.3
2,868,213 - NIAGMN_14410 0.51 +0.8
2,868,213 - NIAGMN_14410 0.51 -0.1
2,868,216 + NIAGMN_14410 0.51 +0.4
2,868,216 + NIAGMN_14410 0.51 -1.4
2,868,216 + NIAGMN_14410 0.51 -0.9
2,868,216 + NIAGMN_14410 0.51 -1.0
2,868,216 + NIAGMN_14410 0.51 +0.2
2,868,217 - NIAGMN_14410 0.51 -1.4
2,868,232 + NIAGMN_14410 0.53 +0.9
2,868,249 + NIAGMN_14410 0.54 -1.4
2,868,249 + NIAGMN_14410 0.54 -1.0
2,868,250 - NIAGMN_14410 0.55 -0.5
2,868,252 + NIAGMN_14410 0.55 -1.4
2,868,252 + NIAGMN_14410 0.55 -0.9
2,868,252 + NIAGMN_14410 0.55 +0.9
2,868,253 - NIAGMN_14410 0.55 -0.2
2,868,293 + NIAGMN_14410 0.59 -0.7
2,868,313 - NIAGMN_14410 0.61 -1.9
2,868,314 + NIAGMN_14410 0.61 -2.9
2,868,315 - NIAGMN_14410 0.61 +1.6
2,868,315 - NIAGMN_14410 0.61 +0.6
2,868,315 - NIAGMN_14410 0.61 -0.2
2,868,315 - NIAGMN_14410 0.61 -0.9
2,868,335 + NIAGMN_14410 0.63 +0.7
2,868,336 - NIAGMN_14410 0.63 -1.8
2,868,342 - NIAGMN_14410 0.63 -1.1
2,868,397 - NIAGMN_14410 0.68 -0.1
2,868,436 + NIAGMN_14410 0.72 -1.9
2,868,436 + NIAGMN_14410 0.72 +0.2
2,868,437 - NIAGMN_14410 0.72 -0.7
2,868,437 - NIAGMN_14410 0.72 +0.7
2,868,453 + NIAGMN_14410 0.74 +0.8
2,868,454 - NIAGMN_14410 0.74 -0.4
2,868,454 - NIAGMN_14410 0.74 +0.6
2,868,454 - NIAGMN_14410 0.74 +0.2
2,868,474 + NIAGMN_14410 0.76 -0.0
2,868,474 + NIAGMN_14410 0.76 -0.6
2,868,475 - NIAGMN_14410 0.76 -1.1
2,868,478 - NIAGMN_14410 0.76 +0.2
2,868,490 + NIAGMN_14410 0.77 -2.2
2,868,496 + NIAGMN_14410 0.78 +0.0
2,868,499 + NIAGMN_14410 0.78 +3.5
2,868,500 - NIAGMN_14410 0.78 +0.2
2,868,500 - NIAGMN_14410 0.78 -0.7
2,868,500 - NIAGMN_14410 0.78 +0.2
2,868,500 - NIAGMN_14410 0.78 -0.9
2,868,500 - NIAGMN_14410 0.78 -0.1
2,868,500 - NIAGMN_14410 0.78 -1.9
2,868,520 + NIAGMN_14410 0.80 +0.8
2,868,521 - NIAGMN_14410 0.80 -0.6
2,868,538 + NIAGMN_14410 0.82 -0.5
2,868,543 + NIAGMN_14410 0.82 +2.3
2,868,543 + NIAGMN_14410 0.82 -2.0
2,868,543 + NIAGMN_14410 0.82 +1.0
2,868,543 + NIAGMN_14410 0.82 +0.0
2,868,544 - NIAGMN_14410 0.82 +1.4
2,868,553 + NIAGMN_14410 0.83 +0.1
2,868,554 - NIAGMN_14410 0.83 +0.5
2,868,559 + NIAGMN_14410 0.84 -1.8
2,868,559 + NIAGMN_14410 0.84 -1.4
2,868,559 + NIAGMN_14410 0.84 -0.8
2,868,560 - NIAGMN_14410 0.84 +0.8
2,868,560 - NIAGMN_14410 0.84 -0.3
2,868,560 - NIAGMN_14410 0.84 -0.7
2,868,613 - NIAGMN_14410 0.89 +0.4
2,868,620 + NIAGMN_14410 0.90 -2.1
2,868,621 - NIAGMN_14410 0.90 -1.0
2,868,621 - NIAGMN_14410 0.90 -0.7
2,868,621 - NIAGMN_14410 0.90 +0.9
2,868,632 - +0.6
2,868,632 - +0.0
2,868,635 + +0.8
2,868,635 + -1.4
2,868,636 - -2.4
2,868,636 - +0.2
2,868,709 + +0.1
2,868,726 + -0.2
2,868,726 + -0.5
2,868,726 + -1.1
2,868,726 + -1.0
2,868,726 + +1.1
2,868,726 + +1.2
2,868,726 + +0.1
2,868,727 - +0.9
2,868,727 - +0.1
2,868,748 - +0.6
2,868,764 - -0.0
2,868,801 - -1.8
2,868,899 - NIAGMN_14415 0.26 -1.3
2,868,944 + NIAGMN_14415 0.38 +1.9
2,868,944 + NIAGMN_14415 0.38 +0.4
2,868,944 + NIAGMN_14415 0.38 +0.0
2,868,944 + NIAGMN_14415 0.38 -0.4
2,869,077 + NIAGMN_14415 0.74 -1.5
2,869,138 + NIAGMN_14415 0.90 +0.2
2,869,178 + +0.3
2,869,179 - +0.4
2,869,179 - -0.2
2,869,179 - -0.0
2,869,179 - -0.1
2,869,189 + -0.6
2,869,190 - -3.7
2,869,238 + +0.1
2,869,255 - -0.5
2,869,266 - -0.2
2,869,266 - -0.4
2,869,278 - +2.2
2,869,288 + +0.5
2,869,331 + +0.2
2,869,332 - +0.2
2,869,358 + -2.2
2,869,359 - +0.4
2,869,445 - -0.1
2,869,445 - +0.8
2,869,446 + +0.2
2,869,447 - -1.1
2,869,448 + -0.4
2,869,530 + -0.5
2,869,531 - +0.7
2,869,531 - -1.5
2,869,566 + +0.9
2,869,566 + +0.9
2,869,566 + -0.7
2,869,566 + +0.3
2,869,566 + -0.5
2,869,567 - -0.1
2,869,567 - +0.6
2,869,567 - +1.1
2,869,567 - +0.3
2,869,607 + -0.4
2,869,682 + yhhT NIAGMN_14420 0.11 +0.3
2,869,682 + yhhT NIAGMN_14420 0.11 -1.9
2,869,682 + yhhT NIAGMN_14420 0.11 -2.0
2,869,682 + yhhT NIAGMN_14420 0.11 +0.3
2,869,718 + yhhT NIAGMN_14420 0.14 -0.6
2,869,718 + yhhT NIAGMN_14420 0.14 -1.4
2,869,733 + yhhT NIAGMN_14420 0.16 +0.6
2,869,733 + yhhT NIAGMN_14420 0.16 -0.4
2,869,734 - yhhT NIAGMN_14420 0.16 -0.5
2,869,734 - yhhT NIAGMN_14420 0.16 -0.0
2,869,734 - yhhT NIAGMN_14420 0.16 +0.3
2,869,734 - yhhT NIAGMN_14420 0.16 +0.4
2,869,801 + yhhT NIAGMN_14420 0.22 +0.3
2,869,801 + yhhT NIAGMN_14420 0.22 +0.3
2,869,834 + yhhT NIAGMN_14420 0.25 -0.5
2,869,834 + yhhT NIAGMN_14420 0.25 +1.2
2,869,834 + yhhT NIAGMN_14420 0.25 +0.2
2,869,835 - yhhT NIAGMN_14420 0.25 +2.4
2,869,835 - yhhT NIAGMN_14420 0.25 +2.5
2,869,835 - yhhT NIAGMN_14420 0.25 -0.0
2,869,914 + yhhT NIAGMN_14420 0.33 +0.6
2,869,914 + yhhT NIAGMN_14420 0.33 -1.0
2,869,914 + yhhT NIAGMN_14420 0.33 -0.4
2,869,915 - yhhT NIAGMN_14420 0.33 -1.7
2,869,915 - yhhT NIAGMN_14420 0.33 +1.2
2,869,915 - yhhT NIAGMN_14420 0.33 +0.4
2,869,915 - yhhT NIAGMN_14420 0.33 -0.9
2,869,915 - yhhT NIAGMN_14420 0.33 -1.9
2,869,915 - yhhT NIAGMN_14420 0.33 -1.4
2,869,915 - yhhT NIAGMN_14420 0.33 -0.4
2,869,915 - yhhT NIAGMN_14420 0.33 -2.7
2,869,915 - yhhT NIAGMN_14420 0.33 -0.4
2,869,934 + yhhT NIAGMN_14420 0.35 +0.0
2,869,934 + yhhT NIAGMN_14420 0.35 +1.1
2,869,959 - yhhT NIAGMN_14420 0.37 +0.0
2,870,003 + yhhT NIAGMN_14420 0.41 +1.9
2,870,047 + yhhT NIAGMN_14420 0.46 +0.6
2,870,048 - yhhT NIAGMN_14420 0.46 +0.4
2,870,048 - yhhT NIAGMN_14420 0.46 +0.3
2,870,048 - yhhT NIAGMN_14420 0.46 +0.0
2,870,109 + yhhT NIAGMN_14420 0.52 +0.4
2,870,137 + yhhT NIAGMN_14420 0.54 +1.0
2,870,144 + yhhT NIAGMN_14420 0.55 +0.1
2,870,145 - yhhT NIAGMN_14420 0.55 -0.7

Or see this region's nucleotide sequence