Strain Fitness in Escherichia coli ECRC102 around NIAGMN_13435

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwaaO and rfaY are separated by 25 nucleotidesrfaY and rfaJ are separated by 24 nucleotidesrfaJ and rfaB are separated by 8 nucleotides NIAGMN_13425: waaO - lipopolysaccharide 3-alpha-galactosyltransferase, at 2,652,758 to 2,653,765 waaO NIAGMN_13430: rfaY - lipopolysaccharide core heptose(II) kinase RfaY, at 2,653,791 to 2,654,498 rfaY NIAGMN_13435: rfaJ - glucosyltransferase, at 2,654,523 to 2,655,536 rfaJ NIAGMN_13440: rfaB - UDP-glucose--(glucosyl)LPS alpha-1,2-glucosyltransferase, at 2,655,545 to 2,656,687 rfaB Position (kb) 2654 2655 2656Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2653.542 kb on + strand, within waaOat 2653.542 kb on + strand, within waaOat 2653.547 kb on + strand, within waaOat 2653.547 kb on + strand, within waaOat 2653.547 kb on + strand, within waaOat 2653.663 kb on + strand, within waaOat 2653.913 kb on + strand, within rfaYat 2654.144 kb on + strand, within rfaYat 2654.144 kb on + strand, within rfaYat 2654.390 kb on + strand, within rfaYat 2654.506 kb on - strandat 2654.719 kb on - strand, within rfaJat 2654.832 kb on - strand, within rfaJat 2654.900 kb on - strand, within rfaJat 2654.995 kb on + strand, within rfaJat 2655.059 kb on + strand, within rfaJat 2655.131 kb on - strand, within rfaJat 2655.160 kb on + strand, within rfaJat 2655.321 kb on + strand, within rfaJat 2655.323 kb on + strand, within rfaJat 2655.323 kb on + strand, within rfaJat 2655.360 kb on + strand, within rfaJat 2655.512 kb on + strandat 2655.517 kb on - strandat 2655.706 kb on + strand, within rfaBat 2655.747 kb on + strand, within rfaBat 2655.749 kb on + strand, within rfaBat 2655.876 kb on + strand, within rfaBat 2655.932 kb on - strand, within rfaBat 2656.224 kb on + strand, within rfaBat 2656.241 kb on + strand, within rfaBat 2656.324 kb on + strand, within rfaBat 2656.325 kb on - strand, within rfaBat 2656.361 kb on + strand, within rfaBat 2656.362 kb on - strand, within rfaBat 2656.381 kb on - strand, within rfaBat 2656.435 kb on + strand, within rfaBat 2656.435 kb on + strand, within rfaBat 2656.458 kb on + strand, within rfaBat 2656.465 kb on + strand, within rfaBat 2656.528 kb on + strand, within rfaB

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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2,653,542 + waaO NIAGMN_13425 0.78 -1.6
2,653,542 + waaO NIAGMN_13425 0.78 -0.1
2,653,547 + waaO NIAGMN_13425 0.78 +0.9
2,653,547 + waaO NIAGMN_13425 0.78 +1.3
2,653,547 + waaO NIAGMN_13425 0.78 +0.3
2,653,663 + waaO NIAGMN_13425 0.90 -0.3
2,653,913 + rfaY NIAGMN_13430 0.17 -2.6
2,654,144 + rfaY NIAGMN_13430 0.50 -1.1
2,654,144 + rfaY NIAGMN_13430 0.50 -0.1
2,654,390 + rfaY NIAGMN_13430 0.85 -1.5
2,654,506 - +0.2
2,654,719 - rfaJ NIAGMN_13435 0.19 +1.9
2,654,832 - rfaJ NIAGMN_13435 0.30 -0.4
2,654,900 - rfaJ NIAGMN_13435 0.37 +1.0
2,654,995 + rfaJ NIAGMN_13435 0.47 +2.9
2,655,059 + rfaJ NIAGMN_13435 0.53 -0.9
2,655,131 - rfaJ NIAGMN_13435 0.60 +2.1
2,655,160 + rfaJ NIAGMN_13435 0.63 +0.5
2,655,321 + rfaJ NIAGMN_13435 0.79 +1.2
2,655,323 + rfaJ NIAGMN_13435 0.79 -0.9
2,655,323 + rfaJ NIAGMN_13435 0.79 -1.4
2,655,360 + rfaJ NIAGMN_13435 0.83 +0.5
2,655,512 + +1.2
2,655,517 - -0.2
2,655,706 + rfaB NIAGMN_13440 0.14 +0.6
2,655,747 + rfaB NIAGMN_13440 0.18 +2.9
2,655,749 + rfaB NIAGMN_13440 0.18 +0.7
2,655,876 + rfaB NIAGMN_13440 0.29 -1.2
2,655,932 - rfaB NIAGMN_13440 0.34 -1.9
2,656,224 + rfaB NIAGMN_13440 0.59 +0.1
2,656,241 + rfaB NIAGMN_13440 0.61 +0.1
2,656,324 + rfaB NIAGMN_13440 0.68 +0.8
2,656,325 - rfaB NIAGMN_13440 0.68 +1.0
2,656,361 + rfaB NIAGMN_13440 0.71 +0.6
2,656,362 - rfaB NIAGMN_13440 0.71 -1.9
2,656,381 - rfaB NIAGMN_13440 0.73 -0.8
2,656,435 + rfaB NIAGMN_13440 0.78 +0.3
2,656,435 + rfaB NIAGMN_13440 0.78 -0.2
2,656,458 + rfaB NIAGMN_13440 0.80 +0.6
2,656,465 + rfaB NIAGMN_13440 0.80 +0.9
2,656,528 + rfaB NIAGMN_13440 0.86 +0.1

Or see this region's nucleotide sequence