Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt waaO and rfaY are separated by 25 nucleotides rfaY and rfaJ are separated by 24 nucleotides rfaJ and rfaB are separated by 8 nucleotides
NIAGMN_13425: waaO - lipopolysaccharide 3-alpha-galactosyltransferase, at 2,652,758 to 2,653,765
waaO
NIAGMN_13430: rfaY - lipopolysaccharide core heptose(II) kinase RfaY, at 2,653,791 to 2,654,498
rfaY
NIAGMN_13435: rfaJ - glucosyltransferase, at 2,654,523 to 2,655,536
rfaJ
NIAGMN_13440: rfaB - UDP-glucose--(glucosyl)LPS alpha-1,2-glucosyltransferase, at 2,655,545 to 2,656,687
rfaB
Position (kb)
2654
2655
2656 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2653.542 kb on + strand, within waaO at 2653.542 kb on + strand, within waaO at 2653.547 kb on + strand, within waaO at 2653.547 kb on + strand, within waaO at 2653.547 kb on + strand, within waaO at 2653.663 kb on + strand, within waaO at 2653.913 kb on + strand, within rfaY at 2654.144 kb on + strand, within rfaY at 2654.144 kb on + strand, within rfaY at 2654.390 kb on + strand, within rfaY at 2654.506 kb on - strand at 2654.719 kb on - strand, within rfaJ at 2654.832 kb on - strand, within rfaJ at 2654.900 kb on - strand, within rfaJ at 2654.995 kb on + strand, within rfaJ at 2655.059 kb on + strand, within rfaJ at 2655.131 kb on - strand, within rfaJ at 2655.160 kb on + strand, within rfaJ at 2655.321 kb on + strand, within rfaJ at 2655.323 kb on + strand, within rfaJ at 2655.323 kb on + strand, within rfaJ at 2655.360 kb on + strand, within rfaJ at 2655.512 kb on + strand at 2655.517 kb on - strand at 2655.706 kb on + strand, within rfaB at 2655.747 kb on + strand, within rfaB at 2655.749 kb on + strand, within rfaB at 2655.876 kb on + strand, within rfaB at 2655.932 kb on - strand, within rfaB at 2656.224 kb on + strand, within rfaB at 2656.241 kb on + strand, within rfaB at 2656.324 kb on + strand, within rfaB at 2656.325 kb on - strand, within rfaB at 2656.361 kb on + strand, within rfaB at 2656.362 kb on - strand, within rfaB at 2656.381 kb on - strand, within rfaB at 2656.435 kb on + strand, within rfaB at 2656.435 kb on + strand, within rfaB at 2656.458 kb on + strand, within rfaB at 2656.465 kb on + strand, within rfaB at 2656.528 kb on + strand, within rfaB
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 2,653,542 + waaO NIAGMN_13425 0.78 -1.6 2,653,542 + waaO NIAGMN_13425 0.78 -0.1 2,653,547 + waaO NIAGMN_13425 0.78 +0.9 2,653,547 + waaO NIAGMN_13425 0.78 +1.3 2,653,547 + waaO NIAGMN_13425 0.78 +0.3 2,653,663 + waaO NIAGMN_13425 0.90 -0.3 2,653,913 + rfaY NIAGMN_13430 0.17 -2.6 2,654,144 + rfaY NIAGMN_13430 0.50 -1.1 2,654,144 + rfaY NIAGMN_13430 0.50 -0.1 2,654,390 + rfaY NIAGMN_13430 0.85 -1.5 2,654,506 - +0.2 2,654,719 - rfaJ NIAGMN_13435 0.19 +1.9 2,654,832 - rfaJ NIAGMN_13435 0.30 -0.4 2,654,900 - rfaJ NIAGMN_13435 0.37 +1.0 2,654,995 + rfaJ NIAGMN_13435 0.47 +2.9 2,655,059 + rfaJ NIAGMN_13435 0.53 -0.9 2,655,131 - rfaJ NIAGMN_13435 0.60 +2.1 2,655,160 + rfaJ NIAGMN_13435 0.63 +0.5 2,655,321 + rfaJ NIAGMN_13435 0.79 +1.2 2,655,323 + rfaJ NIAGMN_13435 0.79 -0.9 2,655,323 + rfaJ NIAGMN_13435 0.79 -1.4 2,655,360 + rfaJ NIAGMN_13435 0.83 +0.5 2,655,512 + +1.2 2,655,517 - -0.2 2,655,706 + rfaB NIAGMN_13440 0.14 +0.6 2,655,747 + rfaB NIAGMN_13440 0.18 +2.9 2,655,749 + rfaB NIAGMN_13440 0.18 +0.7 2,655,876 + rfaB NIAGMN_13440 0.29 -1.2 2,655,932 - rfaB NIAGMN_13440 0.34 -1.9 2,656,224 + rfaB NIAGMN_13440 0.59 +0.1 2,656,241 + rfaB NIAGMN_13440 0.61 +0.1 2,656,324 + rfaB NIAGMN_13440 0.68 +0.8 2,656,325 - rfaB NIAGMN_13440 0.68 +1.0 2,656,361 + rfaB NIAGMN_13440 0.71 +0.6 2,656,362 - rfaB NIAGMN_13440 0.71 -1.9 2,656,381 - rfaB NIAGMN_13440 0.73 -0.8 2,656,435 + rfaB NIAGMN_13440 0.78 +0.3 2,656,435 + rfaB NIAGMN_13440 0.78 -0.2 2,656,458 + rfaB NIAGMN_13440 0.80 +0.6 2,656,465 + rfaB NIAGMN_13440 0.80 +0.9 2,656,528 + rfaB NIAGMN_13440 0.86 +0.1
Or see this region's nucleotide sequence