Strain Fitness in Escherichia coli ECRC102 around NIAGMN_13425

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrfaG and rfaP overlap by 8 nucleotidesrfaP and waaO are separated by 42 nucleotideswaaO and rfaY are separated by 25 nucleotidesrfaY and rfaJ are separated by 24 nucleotides NIAGMN_13415: rfaG - Lipopolysaccharide core biosynthesis protein RfaG, at 2,650,801 to 2,651,925 rfaG NIAGMN_13420: rfaP - lipopolysaccharide core heptose(I) kinase RfaP, at 2,651,918 to 2,652,715 rfaP NIAGMN_13425: waaO - lipopolysaccharide 3-alpha-galactosyltransferase, at 2,652,758 to 2,653,765 waaO NIAGMN_13430: rfaY - lipopolysaccharide core heptose(II) kinase RfaY, at 2,653,791 to 2,654,498 rfaY NIAGMN_13435: rfaJ - glucosyltransferase, at 2,654,523 to 2,655,536 rfaJ Position (kb) 2652 2653 2654Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2651.852 kb on - strandat 2651.860 kb on - strandat 2651.860 kb on - strandat 2651.871 kb on - strandat 2651.871 kb on - strandat 2651.873 kb on - strandat 2651.903 kb on + strandat 2651.904 kb on - strandat 2651.980 kb on + strandat 2652.056 kb on + strand, within rfaPat 2652.057 kb on - strand, within rfaPat 2652.190 kb on + strand, within rfaPat 2652.190 kb on + strand, within rfaPat 2652.191 kb on - strand, within rfaPat 2652.194 kb on + strand, within rfaPat 2652.194 kb on + strand, within rfaPat 2652.194 kb on + strand, within rfaPat 2652.194 kb on + strand, within rfaPat 2652.195 kb on - strand, within rfaPat 2652.195 kb on - strand, within rfaPat 2652.247 kb on + strand, within rfaPat 2652.248 kb on - strand, within rfaPat 2652.248 kb on - strand, within rfaPat 2652.276 kb on + strand, within rfaPat 2652.277 kb on - strand, within rfaPat 2652.277 kb on - strand, within rfaPat 2652.338 kb on - strand, within rfaPat 2652.344 kb on - strand, within rfaPat 2652.346 kb on + strand, within rfaPat 2652.346 kb on + strand, within rfaPat 2652.373 kb on + strand, within rfaPat 2652.373 kb on + strand, within rfaPat 2652.407 kb on + strand, within rfaPat 2652.408 kb on - strand, within rfaPat 2652.408 kb on - strand, within rfaPat 2652.410 kb on - strand, within rfaPat 2652.410 kb on - strand, within rfaPat 2652.535 kb on + strand, within rfaPat 2652.754 kb on - strandat 2652.885 kb on + strand, within waaOat 2653.117 kb on + strand, within waaOat 2653.201 kb on + strand, within waaOat 2653.428 kb on + strand, within waaOat 2653.483 kb on + strand, within waaOat 2653.542 kb on + strand, within waaOat 2653.542 kb on + strand, within waaOat 2653.547 kb on + strand, within waaOat 2653.547 kb on + strand, within waaOat 2653.547 kb on + strand, within waaOat 2653.663 kb on + strand, within waaOat 2653.913 kb on + strand, within rfaYat 2654.144 kb on + strand, within rfaYat 2654.144 kb on + strand, within rfaYat 2654.390 kb on + strand, within rfaYat 2654.506 kb on - strandat 2654.719 kb on - strand, within rfaJ

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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2,651,852 - +0.6
2,651,860 - +1.6
2,651,860 - -2.0
2,651,871 - +0.5
2,651,871 - -0.9
2,651,873 - -1.5
2,651,903 + +1.1
2,651,904 - +0.4
2,651,980 + +0.0
2,652,056 + rfaP NIAGMN_13420 0.17 -1.8
2,652,057 - rfaP NIAGMN_13420 0.17 -1.7
2,652,190 + rfaP NIAGMN_13420 0.34 +2.1
2,652,190 + rfaP NIAGMN_13420 0.34 -0.6
2,652,191 - rfaP NIAGMN_13420 0.34 +2.3
2,652,194 + rfaP NIAGMN_13420 0.35 -1.1
2,652,194 + rfaP NIAGMN_13420 0.35 -1.5
2,652,194 + rfaP NIAGMN_13420 0.35 -1.8
2,652,194 + rfaP NIAGMN_13420 0.35 -1.0
2,652,195 - rfaP NIAGMN_13420 0.35 +0.9
2,652,195 - rfaP NIAGMN_13420 0.35 -1.3
2,652,247 + rfaP NIAGMN_13420 0.41 +2.2
2,652,248 - rfaP NIAGMN_13420 0.41 +0.5
2,652,248 - rfaP NIAGMN_13420 0.41 -0.7
2,652,276 + rfaP NIAGMN_13420 0.45 -0.4
2,652,277 - rfaP NIAGMN_13420 0.45 -0.6
2,652,277 - rfaP NIAGMN_13420 0.45 -0.6
2,652,338 - rfaP NIAGMN_13420 0.53 +1.5
2,652,344 - rfaP NIAGMN_13420 0.53 +2.0
2,652,346 + rfaP NIAGMN_13420 0.54 +0.6
2,652,346 + rfaP NIAGMN_13420 0.54 -0.9
2,652,373 + rfaP NIAGMN_13420 0.57 -1.0
2,652,373 + rfaP NIAGMN_13420 0.57 +0.1
2,652,407 + rfaP NIAGMN_13420 0.61 -0.1
2,652,408 - rfaP NIAGMN_13420 0.61 +1.5
2,652,408 - rfaP NIAGMN_13420 0.61 -1.2
2,652,410 - rfaP NIAGMN_13420 0.62 +0.1
2,652,410 - rfaP NIAGMN_13420 0.62 -0.6
2,652,535 + rfaP NIAGMN_13420 0.77 -0.8
2,652,754 - -1.2
2,652,885 + waaO NIAGMN_13425 0.13 -0.2
2,653,117 + waaO NIAGMN_13425 0.36 +0.6
2,653,201 + waaO NIAGMN_13425 0.44 +0.4
2,653,428 + waaO NIAGMN_13425 0.66 +0.2
2,653,483 + waaO NIAGMN_13425 0.72 -2.9
2,653,542 + waaO NIAGMN_13425 0.78 -1.6
2,653,542 + waaO NIAGMN_13425 0.78 -0.1
2,653,547 + waaO NIAGMN_13425 0.78 +0.9
2,653,547 + waaO NIAGMN_13425 0.78 +1.3
2,653,547 + waaO NIAGMN_13425 0.78 +0.3
2,653,663 + waaO NIAGMN_13425 0.90 -0.3
2,653,913 + rfaY NIAGMN_13430 0.17 -2.6
2,654,144 + rfaY NIAGMN_13430 0.50 -1.1
2,654,144 + rfaY NIAGMN_13430 0.50 -0.1
2,654,390 + rfaY NIAGMN_13430 0.85 -1.5
2,654,506 - +0.2
2,654,719 - rfaJ NIAGMN_13435 0.19 +1.9

Or see this region's nucleotide sequence