Strain Fitness in Escherichia coli ECRC102 around NIAGMN_09720

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpyrB and pyrI are separated by 12 nucleotidespyrI and ridA are separated by 72 nucleotidesridA and mgtA are separated by 205 nucleotides NIAGMN_09715: pyrB - aspartate carbamoyltransferase, at 1,912,545 to 1,913,480 pyrB NIAGMN_09720: pyrI - aspartate carbamoyltransferase regulatory subunit, at 1,913,493 to 1,913,954 pyrI NIAGMN_09725: ridA - 2-iminobutanoate/2-iminopropanoate deaminase, at 1,914,027 to 1,914,413 ridA NIAGMN_09730: mgtA - magnesium-translocating P-type ATPase, at 1,914,619 to 1,917,315 mgtA Position (kb) 1913 1914Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1 2 3at 1912.540 kb on - strandat 1912.559 kb on - strandat 1912.561 kb on - strandat 1912.561 kb on - strandat 1912.572 kb on - strandat 1912.572 kb on - strandat 1912.592 kb on + strandat 1912.781 kb on + strand, within pyrBat 1912.781 kb on + strand, within pyrBat 1912.839 kb on + strand, within pyrBat 1912.839 kb on + strand, within pyrBat 1912.839 kb on + strand, within pyrBat 1912.839 kb on + strand, within pyrBat 1912.839 kb on + strand, within pyrBat 1912.839 kb on + strand, within pyrBat 1912.840 kb on - strand, within pyrBat 1912.853 kb on - strand, within pyrBat 1912.912 kb on + strand, within pyrBat 1912.912 kb on + strand, within pyrBat 1912.912 kb on + strand, within pyrBat 1912.912 kb on + strand, within pyrBat 1912.913 kb on - strand, within pyrBat 1912.913 kb on - strand, within pyrBat 1912.972 kb on + strand, within pyrBat 1912.972 kb on + strand, within pyrBat 1912.973 kb on - strand, within pyrBat 1912.973 kb on - strand, within pyrBat 1912.973 kb on - strand, within pyrBat 1913.040 kb on + strand, within pyrBat 1913.040 kb on + strand, within pyrBat 1913.100 kb on + strand, within pyrBat 1913.101 kb on - strand, within pyrBat 1913.101 kb on - strand, within pyrBat 1913.136 kb on + strand, within pyrBat 1913.137 kb on - strand, within pyrBat 1913.137 kb on - strand, within pyrBat 1913.137 kb on - strand, within pyrBat 1913.189 kb on + strand, within pyrBat 1913.223 kb on + strand, within pyrBat 1913.223 kb on + strand, within pyrBat 1913.223 kb on + strand, within pyrBat 1913.224 kb on - strand, within pyrBat 1913.224 kb on - strand, within pyrBat 1913.328 kb on - strand, within pyrBat 1913.335 kb on + strand, within pyrBat 1913.336 kb on - strand, within pyrBat 1913.381 kb on + strand, within pyrBat 1913.381 kb on + strand, within pyrBat 1913.382 kb on - strand, within pyrBat 1913.382 kb on - strand, within pyrBat 1913.400 kb on + strandat 1913.400 kb on + strandat 1913.473 kb on + strandat 1913.478 kb on + strandat 1913.479 kb on - strandat 1913.488 kb on + strandat 1913.488 kb on + strandat 1913.488 kb on + strandat 1913.507 kb on + strandat 1913.542 kb on + strand, within pyrIat 1913.543 kb on - strand, within pyrIat 1913.573 kb on + strand, within pyrIat 1913.573 kb on + strand, within pyrIat 1913.704 kb on + strand, within pyrIat 1913.705 kb on - strand, within pyrIat 1913.705 kb on - strand, within pyrIat 1913.705 kb on - strand, within pyrIat 1913.705 kb on - strand, within pyrIat 1913.721 kb on + strand, within pyrIat 1913.721 kb on + strand, within pyrIat 1913.722 kb on - strand, within pyrIat 1913.757 kb on + strand, within pyrIat 1913.757 kb on + strand, within pyrIat 1913.771 kb on + strand, within pyrIat 1913.772 kb on - strand, within pyrIat 1913.834 kb on + strand, within pyrIat 1913.834 kb on + strand, within pyrIat 1913.834 kb on + strand, within pyrIat 1913.834 kb on + strand, within pyrIat 1913.835 kb on - strand, within pyrIat 1913.910 kb on + strandat 1913.911 kb on - strandat 1913.933 kb on + strandat 1913.952 kb on + strandat 1913.965 kb on + strandat 1913.965 kb on + strandat 1913.968 kb on + strandat 1913.968 kb on + strandat 1913.984 kb on + strandat 1913.984 kb on + strandat 1913.985 kb on - strandat 1913.987 kb on - strandat 1913.987 kb on - strandat 1914.011 kb on + strandat 1914.011 kb on + strandat 1914.012 kb on - strandat 1914.014 kb on + strandat 1914.066 kb on - strand, within ridAat 1914.075 kb on + strand, within ridAat 1914.075 kb on + strand, within ridAat 1914.076 kb on - strand, within ridAat 1914.079 kb on + strand, within ridAat 1914.080 kb on - strand, within ridAat 1914.080 kb on - strand, within ridAat 1914.080 kb on - strand, within ridAat 1914.101 kb on + strand, within ridAat 1914.101 kb on + strand, within ridAat 1914.101 kb on + strand, within ridAat 1914.101 kb on + strand, within ridAat 1914.101 kb on + strand, within ridAat 1914.102 kb on - strand, within ridAat 1914.102 kb on - strand, within ridAat 1914.102 kb on - strand, within ridAat 1914.260 kb on - strand, within ridAat 1914.297 kb on + strand, within ridAat 1914.297 kb on + strand, within ridAat 1914.298 kb on - strand, within ridAat 1914.433 kb on + strandat 1914.447 kb on + strandat 1914.447 kb on + strandat 1914.448 kb on - strandat 1914.545 kb on + strandat 1914.644 kb on + strandat 1914.644 kb on + strandat 1914.644 kb on + strandat 1914.645 kb on - strandat 1914.645 kb on - strandat 1914.645 kb on - strandat 1914.675 kb on + strandat 1914.675 kb on + strandat 1914.676 kb on - strandat 1914.677 kb on + strandat 1914.677 kb on + strandat 1914.678 kb on - strandat 1914.678 kb on - strandat 1914.678 kb on - strandat 1914.678 kb on - strandat 1914.678 kb on - strandat 1914.678 kb on - strandat 1914.678 kb on - strandat 1914.678 kb on - strandat 1914.678 kb on - strandat 1914.710 kb on + strandat 1914.711 kb on - strandat 1914.711 kb on - strandat 1914.711 kb on - strandat 1914.740 kb on + strandat 1914.741 kb on - strandat 1914.832 kb on - strandat 1914.858 kb on + strandat 1914.859 kb on - strandat 1914.947 kb on - strand, within mgtA

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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1,912,540 - -2.9
1,912,559 - -2.3
1,912,561 - -3.4
1,912,561 - -2.0
1,912,572 - -4.0
1,912,572 - -4.4
1,912,592 + -3.7
1,912,781 + pyrB NIAGMN_09715 0.25 -4.0
1,912,781 + pyrB NIAGMN_09715 0.25 -4.4
1,912,839 + pyrB NIAGMN_09715 0.31 -2.1
1,912,839 + pyrB NIAGMN_09715 0.31 -2.8
1,912,839 + pyrB NIAGMN_09715 0.31 -4.5
1,912,839 + pyrB NIAGMN_09715 0.31 -3.1
1,912,839 + pyrB NIAGMN_09715 0.31 -0.9
1,912,839 + pyrB NIAGMN_09715 0.31 -2.7
1,912,840 - pyrB NIAGMN_09715 0.32 -2.7
1,912,853 - pyrB NIAGMN_09715 0.33 -2.0
1,912,912 + pyrB NIAGMN_09715 0.39 -1.3
1,912,912 + pyrB NIAGMN_09715 0.39 -3.4
1,912,912 + pyrB NIAGMN_09715 0.39 +0.4
1,912,912 + pyrB NIAGMN_09715 0.39 -1.1
1,912,913 - pyrB NIAGMN_09715 0.39 -4.2
1,912,913 - pyrB NIAGMN_09715 0.39 -3.8
1,912,972 + pyrB NIAGMN_09715 0.46 -1.9
1,912,972 + pyrB NIAGMN_09715 0.46 -1.5
1,912,973 - pyrB NIAGMN_09715 0.46 -1.6
1,912,973 - pyrB NIAGMN_09715 0.46 -0.4
1,912,973 - pyrB NIAGMN_09715 0.46 -2.6
1,913,040 + pyrB NIAGMN_09715 0.53 -2.8
1,913,040 + pyrB NIAGMN_09715 0.53 -4.8
1,913,100 + pyrB NIAGMN_09715 0.59 -3.1
1,913,101 - pyrB NIAGMN_09715 0.59 -3.4
1,913,101 - pyrB NIAGMN_09715 0.59 -4.2
1,913,136 + pyrB NIAGMN_09715 0.63 -3.0
1,913,137 - pyrB NIAGMN_09715 0.63 -2.1
1,913,137 - pyrB NIAGMN_09715 0.63 -6.7
1,913,137 - pyrB NIAGMN_09715 0.63 -3.5
1,913,189 + pyrB NIAGMN_09715 0.69 -2.8
1,913,223 + pyrB NIAGMN_09715 0.72 -2.3
1,913,223 + pyrB NIAGMN_09715 0.72 -4.7
1,913,223 + pyrB NIAGMN_09715 0.72 -2.6
1,913,224 - pyrB NIAGMN_09715 0.73 -2.9
1,913,224 - pyrB NIAGMN_09715 0.73 -1.1
1,913,328 - pyrB NIAGMN_09715 0.84 -1.9
1,913,335 + pyrB NIAGMN_09715 0.84 -5.0
1,913,336 - pyrB NIAGMN_09715 0.85 -4.1
1,913,381 + pyrB NIAGMN_09715 0.89 -3.6
1,913,381 + pyrB NIAGMN_09715 0.89 -2.2
1,913,382 - pyrB NIAGMN_09715 0.89 -0.9
1,913,382 - pyrB NIAGMN_09715 0.89 -0.6
1,913,400 + -3.3
1,913,400 + -2.4
1,913,473 + +3.4
1,913,478 + +0.0
1,913,479 - +1.1
1,913,488 + +1.0
1,913,488 + +0.8
1,913,488 + -1.5
1,913,507 + -3.8
1,913,542 + pyrI NIAGMN_09720 0.11 -0.6
1,913,543 - pyrI NIAGMN_09720 0.11 +0.7
1,913,573 + pyrI NIAGMN_09720 0.17 +0.4
1,913,573 + pyrI NIAGMN_09720 0.17 -1.4
1,913,704 + pyrI NIAGMN_09720 0.46 +0.2
1,913,705 - pyrI NIAGMN_09720 0.46 +0.4
1,913,705 - pyrI NIAGMN_09720 0.46 -1.5
1,913,705 - pyrI NIAGMN_09720 0.46 -0.1
1,913,705 - pyrI NIAGMN_09720 0.46 +1.6
1,913,721 + pyrI NIAGMN_09720 0.49 -1.8
1,913,721 + pyrI NIAGMN_09720 0.49 +0.7
1,913,722 - pyrI NIAGMN_09720 0.50 -0.8
1,913,757 + pyrI NIAGMN_09720 0.57 -1.5
1,913,757 + pyrI NIAGMN_09720 0.57 +0.3
1,913,771 + pyrI NIAGMN_09720 0.60 +1.0
1,913,772 - pyrI NIAGMN_09720 0.60 -0.8
1,913,834 + pyrI NIAGMN_09720 0.74 -0.0
1,913,834 + pyrI NIAGMN_09720 0.74 -0.5
1,913,834 + pyrI NIAGMN_09720 0.74 +0.2
1,913,834 + pyrI NIAGMN_09720 0.74 +0.2
1,913,835 - pyrI NIAGMN_09720 0.74 -0.4
1,913,910 + +0.2
1,913,911 - -0.6
1,913,933 + -0.4
1,913,952 + -0.9
1,913,965 + +0.1
1,913,965 + +1.4
1,913,968 + -0.2
1,913,968 + +1.8
1,913,984 + +0.6
1,913,984 + +1.9
1,913,985 - +0.8
1,913,987 - +1.4
1,913,987 - -1.0
1,914,011 + +0.4
1,914,011 + -1.8
1,914,012 - -0.1
1,914,014 + +0.1
1,914,066 - ridA NIAGMN_09725 0.10 +1.3
1,914,075 + ridA NIAGMN_09725 0.12 +0.7
1,914,075 + ridA NIAGMN_09725 0.12 -0.4
1,914,076 - ridA NIAGMN_09725 0.13 +0.3
1,914,079 + ridA NIAGMN_09725 0.13 +0.8
1,914,080 - ridA NIAGMN_09725 0.14 -2.7
1,914,080 - ridA NIAGMN_09725 0.14 -0.6
1,914,080 - ridA NIAGMN_09725 0.14 -0.3
1,914,101 + ridA NIAGMN_09725 0.19 -1.8
1,914,101 + ridA NIAGMN_09725 0.19 -1.0
1,914,101 + ridA NIAGMN_09725 0.19 -1.8
1,914,101 + ridA NIAGMN_09725 0.19 +0.6
1,914,101 + ridA NIAGMN_09725 0.19 +0.8
1,914,102 - ridA NIAGMN_09725 0.19 +1.0
1,914,102 - ridA NIAGMN_09725 0.19 +0.3
1,914,102 - ridA NIAGMN_09725 0.19 +0.5
1,914,260 - ridA NIAGMN_09725 0.60 -1.7
1,914,297 + ridA NIAGMN_09725 0.70 -1.6
1,914,297 + ridA NIAGMN_09725 0.70 +0.2
1,914,298 - ridA NIAGMN_09725 0.70 -1.8
1,914,433 + -0.3
1,914,447 + -0.7
1,914,447 + -1.7
1,914,448 - -0.1
1,914,545 + -1.5
1,914,644 + -0.8
1,914,644 + +0.2
1,914,644 + -2.0
1,914,645 - +0.9
1,914,645 - +0.3
1,914,645 - +0.4
1,914,675 + -1.2
1,914,675 + -0.1
1,914,676 - -1.0
1,914,677 + -0.7
1,914,677 + +0.1
1,914,678 - -0.6
1,914,678 - -0.4
1,914,678 - -0.4
1,914,678 - -0.6
1,914,678 - -1.1
1,914,678 - -2.3
1,914,678 - -2.4
1,914,678 - +2.0
1,914,678 - -0.0
1,914,710 + -1.0
1,914,711 - -1.4
1,914,711 - -1.0
1,914,711 - -0.0
1,914,740 + +1.7
1,914,741 - -1.1
1,914,832 - -1.6
1,914,858 + -0.7
1,914,859 - -1.4
1,914,947 - mgtA NIAGMN_09730 0.12 -1.7

Or see this region's nucleotide sequence