Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rcsF and tsaA overlap by 4 nucleotides tsaA and proS are separated by 110 nucleotides proS and yaeF are separated by 52 nucleotides
NIAGMN_07915: rcsF - Rcs stress response system protein RcsF, at 1,518,719 to 1,519,123
rcsF
NIAGMN_07920: tsaA - tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO, at 1,519,120 to 1,519,827
tsaA
NIAGMN_07925: proS - proline--tRNA ligase, at 1,519,938 to 1,521,656
proS
NIAGMN_07930: yaeF - YaeF family permuted papain-like enzyme, at 1,521,709 to 1,522,533
yaeF
Position (kb)
1519
1520
1521
1522 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1518.949 kb on + strand, within rcsF at 1519.032 kb on + strand, within rcsF at 1519.032 kb on + strand, within rcsF at 1519.032 kb on + strand, within rcsF at 1519.039 kb on + strand, within rcsF at 1519.040 kb on - strand, within rcsF at 1519.051 kb on + strand, within rcsF at 1519.051 kb on + strand, within rcsF at 1519.086 kb on + strand at 1519.086 kb on + strand at 1519.086 kb on + strand at 1519.086 kb on + strand at 1519.086 kb on + strand at 1519.087 kb on - strand at 1519.087 kb on - strand at 1519.105 kb on + strand at 1519.106 kb on - strand at 1519.111 kb on + strand at 1519.145 kb on + strand at 1519.146 kb on - strand at 1519.165 kb on + strand at 1519.166 kb on - strand at 1519.167 kb on + strand at 1519.205 kb on + strand, within tsaA at 1519.227 kb on - strand, within tsaA at 1519.244 kb on - strand, within tsaA at 1519.289 kb on + strand, within tsaA at 1519.289 kb on + strand, within tsaA at 1519.290 kb on - strand, within tsaA at 1519.290 kb on - strand, within tsaA at 1519.422 kb on + strand, within tsaA at 1519.423 kb on - strand, within tsaA at 1519.423 kb on - strand, within tsaA at 1519.423 kb on - strand, within tsaA at 1519.423 kb on - strand, within tsaA at 1519.434 kb on - strand, within tsaA at 1519.434 kb on - strand, within tsaA at 1519.531 kb on + strand, within tsaA at 1519.531 kb on + strand, within tsaA at 1519.573 kb on + strand, within tsaA at 1519.574 kb on - strand, within tsaA at 1519.688 kb on + strand, within tsaA at 1519.720 kb on - strand, within tsaA at 1519.744 kb on + strand, within tsaA at 1519.767 kb on + strand at 1519.826 kb on - strand at 1519.871 kb on + strand at 1519.871 kb on + strand at 1519.871 kb on + strand at 1519.871 kb on + strand at 1521.759 kb on - strand at 1521.781 kb on - strand at 1521.812 kb on + strand, within yaeF at 1521.813 kb on - strand, within yaeF at 1521.813 kb on - strand, within yaeF at 1521.888 kb on - strand, within yaeF at 1521.888 kb on - strand, within yaeF at 1521.888 kb on - strand, within yaeF at 1521.888 kb on - strand, within yaeF at 1521.942 kb on - strand, within yaeF at 1521.998 kb on - strand, within yaeF at 1521.998 kb on - strand, within yaeF at 1522.012 kb on - strand, within yaeF at 1522.074 kb on + strand, within yaeF at 1522.074 kb on + strand, within yaeF at 1522.094 kb on + strand, within yaeF at 1522.094 kb on + strand, within yaeF at 1522.170 kb on + strand, within yaeF at 1522.170 kb on + strand, within yaeF at 1522.170 kb on + strand, within yaeF at 1522.170 kb on + strand, within yaeF at 1522.171 kb on - strand, within yaeF at 1522.203 kb on + strand, within yaeF at 1522.290 kb on - strand, within yaeF at 1522.374 kb on + strand, within yaeF at 1522.398 kb on + strand, within yaeF at 1522.419 kb on + strand, within yaeF at 1522.425 kb on + strand, within yaeF at 1522.463 kb on + strand at 1522.463 kb on + strand at 1522.464 kb on - strand at 1522.468 kb on + strand at 1522.468 kb on + strand at 1522.469 kb on - strand at 1522.495 kb on + strand at 1522.495 kb on + strand at 1522.495 kb on + strand at 1522.496 kb on - strand at 1522.496 kb on - strand at 1522.496 kb on - strand at 1522.497 kb on + strand at 1522.498 kb on - strand at 1522.534 kb on + strand at 1522.539 kb on + strand at 1522.539 kb on + strand at 1522.539 kb on + strand at 1522.562 kb on + strand at 1522.563 kb on - strand at 1522.585 kb on + strand at 1522.585 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 1,518,949 + rcsF NIAGMN_07915 0.57 +1.0 1,519,032 + rcsF NIAGMN_07915 0.77 +2.6 1,519,032 + rcsF NIAGMN_07915 0.77 -1.4 1,519,032 + rcsF NIAGMN_07915 0.77 +0.6 1,519,039 + rcsF NIAGMN_07915 0.79 -1.0 1,519,040 - rcsF NIAGMN_07915 0.79 +0.6 1,519,051 + rcsF NIAGMN_07915 0.82 -1.2 1,519,051 + rcsF NIAGMN_07915 0.82 -1.4 1,519,086 + -0.2 1,519,086 + +0.1 1,519,086 + -0.2 1,519,086 + +3.3 1,519,086 + -0.5 1,519,087 - +0.9 1,519,087 - -1.7 1,519,105 + -0.3 1,519,106 - -0.3 1,519,111 + +0.5 1,519,145 + +0.4 1,519,146 - +0.3 1,519,165 + -0.6 1,519,166 - -0.6 1,519,167 + -0.5 1,519,205 + tsaA NIAGMN_07920 0.12 +0.4 1,519,227 - tsaA NIAGMN_07920 0.15 +0.7 1,519,244 - tsaA NIAGMN_07920 0.18 +1.3 1,519,289 + tsaA NIAGMN_07920 0.24 -0.9 1,519,289 + tsaA NIAGMN_07920 0.24 +0.2 1,519,290 - tsaA NIAGMN_07920 0.24 +0.2 1,519,290 - tsaA NIAGMN_07920 0.24 +2.6 1,519,422 + tsaA NIAGMN_07920 0.43 +0.1 1,519,423 - tsaA NIAGMN_07920 0.43 -0.7 1,519,423 - tsaA NIAGMN_07920 0.43 -3.3 1,519,423 - tsaA NIAGMN_07920 0.43 +3.1 1,519,423 - tsaA NIAGMN_07920 0.43 -0.3 1,519,434 - tsaA NIAGMN_07920 0.44 -0.2 1,519,434 - tsaA NIAGMN_07920 0.44 +0.6 1,519,531 + tsaA NIAGMN_07920 0.58 +0.6 1,519,531 + tsaA NIAGMN_07920 0.58 -0.8 1,519,573 + tsaA NIAGMN_07920 0.64 -0.7 1,519,574 - tsaA NIAGMN_07920 0.64 -0.0 1,519,688 + tsaA NIAGMN_07920 0.80 +0.7 1,519,720 - tsaA NIAGMN_07920 0.85 -2.9 1,519,744 + tsaA NIAGMN_07920 0.88 +1.1 1,519,767 + -0.1 1,519,826 - +0.5 1,519,871 + +1.3 1,519,871 + -0.8 1,519,871 + -0.2 1,519,871 + -0.5 1,521,759 - +0.6 1,521,781 - +0.0 1,521,812 + yaeF NIAGMN_07930 0.12 -0.5 1,521,813 - yaeF NIAGMN_07930 0.13 +0.1 1,521,813 - yaeF NIAGMN_07930 0.13 +0.5 1,521,888 - yaeF NIAGMN_07930 0.22 -1.9 1,521,888 - yaeF NIAGMN_07930 0.22 +1.3 1,521,888 - yaeF NIAGMN_07930 0.22 +0.9 1,521,888 - yaeF NIAGMN_07930 0.22 +1.0 1,521,942 - yaeF NIAGMN_07930 0.28 +0.8 1,521,998 - yaeF NIAGMN_07930 0.35 -2.4 1,521,998 - yaeF NIAGMN_07930 0.35 -2.8 1,522,012 - yaeF NIAGMN_07930 0.37 +0.7 1,522,074 + yaeF NIAGMN_07930 0.44 +0.1 1,522,074 + yaeF NIAGMN_07930 0.44 -1.6 1,522,094 + yaeF NIAGMN_07930 0.47 +3.0 1,522,094 + yaeF NIAGMN_07930 0.47 -0.8 1,522,170 + yaeF NIAGMN_07930 0.56 -0.9 1,522,170 + yaeF NIAGMN_07930 0.56 -0.0 1,522,170 + yaeF NIAGMN_07930 0.56 +3.2 1,522,170 + yaeF NIAGMN_07930 0.56 +2.0 1,522,171 - yaeF NIAGMN_07930 0.56 +0.3 1,522,203 + yaeF NIAGMN_07930 0.60 -0.6 1,522,290 - yaeF NIAGMN_07930 0.70 +0.4 1,522,374 + yaeF NIAGMN_07930 0.81 -0.6 1,522,398 + yaeF NIAGMN_07930 0.84 -0.9 1,522,419 + yaeF NIAGMN_07930 0.86 +0.4 1,522,425 + yaeF NIAGMN_07930 0.87 +1.8 1,522,463 + -1.6 1,522,463 + +3.2 1,522,464 - -0.1 1,522,468 + -0.9 1,522,468 + +0.1 1,522,469 - -2.0 1,522,495 + -1.0 1,522,495 + -1.2 1,522,495 + +0.5 1,522,496 - +1.6 1,522,496 - +0.9 1,522,496 - +3.7 1,522,497 + +1.6 1,522,498 - +0.4 1,522,534 + -2.0 1,522,539 + +0.6 1,522,539 + +0.5 1,522,539 + +1.3 1,522,562 + -0.1 1,522,563 - +0.6 1,522,585 + -0.4 1,522,585 + -0.1
Or see this region's nucleotide sequence