Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dkgB and Asp_trna are separated by 162 nucleotides Asp_trna and 5S_rrna are separated by 54 nucleotides 5S_rrna and 23S_rrna are separated by 94 nucleotides 23S_rrna and Ala_trna are separated by 183 nucleotides Ala_trna and Ile_trna are separated by 42 nucleotides Ile_trna and 16S_rrna are separated by 68 nucleotides
NIAGMN_07855: dkgB - 2,5-didehydrogluconate reductase DkgB, at 1,508,745 to 1,509,548
dkgB
NIAGMN_07860: Asp_trna - tRNA-Asp(gtc), at 1,509,711 to 1,509,787
_trna
NIAGMN_07865: 5S_rrna - 5S ribosomal RNA, at 1,509,842 to 1,509,957
_rrna
NIAGMN_07870: 23S_rrna - 23S ribosomal RNA, at 1,510,052 to 1,512,954
_rrna
NIAGMN_07880: Ala_trna - tRNA-Ala(tgc), at 1,513,138 to 1,513,213
_trna
NIAGMN_07885: Ile_trna - tRNA-Ile(gat), at 1,513,256 to 1,513,332
_trna
NIAGMN_07890: 16S_rrna - 16S ribosomal RNA, at 1,513,401 to 1,514,942
_rrna
Position (kb) 1510
1511
1512
1513 Strain fitness (log2 ratio) -1
0
1 at 1509.280 kb on + strand, within dkgB at 1509.414 kb on + strand, within dkgB at 1509.548 kb on + strand at 1509.548 kb on + strand at 1509.552 kb on - strand at 1509.569 kb on - strand at 1509.666 kb on - strand at 1509.705 kb on - strand at 1509.708 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 1,509,280 + dkgB NIAGMN_07855 0.67 -0.2 1,509,414 + dkgB NIAGMN_07855 0.83 -0.2 1,509,548 + -1.3 1,509,548 + -0.1 1,509,552 - -0.5 1,509,569 - -1.2 1,509,666 - +0.5 1,509,705 - -1.1 1,509,708 - +0.4
Or see this region's nucleotide sequence