Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fdrA and allD are separated by 316 nucleotides allD and allC are separated by 21 nucleotides
NIAGMN_05915: fdrA - Protein FdrA, at 1,100,185 to 1,101,852
fdrA
NIAGMN_05920: allD - ureidoglycolate dehydrogenase, at 1,102,169 to 1,103,218
allD
NIAGMN_05925: allC - allantoate deiminase, at 1,103,240 to 1,104,475
allC
Position (kb) 1102
1103
1104 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1101.325 kb on - strand, within fdrA at 1101.610 kb on - strand, within fdrA at 1101.712 kb on - strand at 1101.716 kb on - strand at 1101.716 kb on - strand at 1101.900 kb on - strand at 1102.101 kb on + strand at 1102.200 kb on + strand at 1102.319 kb on + strand, within allD at 1102.404 kb on + strand, within allD at 1102.404 kb on + strand, within allD at 1102.404 kb on + strand, within allD at 1102.626 kb on - strand, within allD at 1102.628 kb on + strand, within allD at 1102.633 kb on + strand, within allD at 1102.636 kb on + strand, within allD at 1102.681 kb on + strand, within allD at 1102.744 kb on + strand, within allD at 1102.744 kb on + strand, within allD at 1102.744 kb on + strand, within allD at 1102.744 kb on + strand, within allD at 1102.744 kb on + strand, within allD at 1102.744 kb on + strand, within allD at 1102.744 kb on + strand, within allD at 1102.745 kb on - strand, within allD at 1102.809 kb on + strand, within allD at 1102.810 kb on - strand, within allD at 1102.810 kb on - strand, within allD at 1102.810 kb on - strand, within allD at 1102.923 kb on - strand, within allD at 1102.960 kb on - strand, within allD at 1102.966 kb on - strand, within allD at 1103.189 kb on + strand at 1103.245 kb on + strand at 1103.265 kb on + strand at 1103.769 kb on + strand, within allC at 1103.836 kb on + strand, within allC at 1103.837 kb on - strand, within allC at 1103.887 kb on + strand, within allC at 1103.957 kb on + strand, within allC at 1103.958 kb on - strand, within allC at 1104.144 kb on + strand, within allC at 1104.144 kb on + strand, within allC at 1104.201 kb on + strand, within allC at 1104.201 kb on + strand, within allC
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 1,101,325 - fdrA NIAGMN_05915 0.68 +0.5 1,101,610 - fdrA NIAGMN_05915 0.85 -0.0 1,101,712 - -2.5 1,101,716 - -0.7 1,101,716 - +0.5 1,101,900 - +0.3 1,102,101 + +1.4 1,102,200 + +1.5 1,102,319 + allD NIAGMN_05920 0.14 +1.6 1,102,404 + allD NIAGMN_05920 0.22 +1.4 1,102,404 + allD NIAGMN_05920 0.22 +0.0 1,102,404 + allD NIAGMN_05920 0.22 -1.9 1,102,626 - allD NIAGMN_05920 0.44 -0.7 1,102,628 + allD NIAGMN_05920 0.44 -2.8 1,102,633 + allD NIAGMN_05920 0.44 -1.3 1,102,636 + allD NIAGMN_05920 0.44 +1.0 1,102,681 + allD NIAGMN_05920 0.49 +0.0 1,102,744 + allD NIAGMN_05920 0.55 +0.9 1,102,744 + allD NIAGMN_05920 0.55 -0.4 1,102,744 + allD NIAGMN_05920 0.55 +0.5 1,102,744 + allD NIAGMN_05920 0.55 +3.3 1,102,744 + allD NIAGMN_05920 0.55 +0.2 1,102,744 + allD NIAGMN_05920 0.55 -2.1 1,102,744 + allD NIAGMN_05920 0.55 -0.2 1,102,745 - allD NIAGMN_05920 0.55 +0.3 1,102,809 + allD NIAGMN_05920 0.61 +1.6 1,102,810 - allD NIAGMN_05920 0.61 +0.0 1,102,810 - allD NIAGMN_05920 0.61 +1.0 1,102,810 - allD NIAGMN_05920 0.61 +0.3 1,102,923 - allD NIAGMN_05920 0.72 +0.4 1,102,960 - allD NIAGMN_05920 0.75 +2.3 1,102,966 - allD NIAGMN_05920 0.76 -1.5 1,103,189 + +0.3 1,103,245 + -1.1 1,103,265 + -0.4 1,103,769 + allC NIAGMN_05925 0.43 -0.4 1,103,836 + allC NIAGMN_05925 0.48 +3.4 1,103,837 - allC NIAGMN_05925 0.48 -0.1 1,103,887 + allC NIAGMN_05925 0.52 +1.4 1,103,957 + allC NIAGMN_05925 0.58 +1.1 1,103,958 - allC NIAGMN_05925 0.58 -0.5 1,104,144 + allC NIAGMN_05925 0.73 -0.9 1,104,144 + allC NIAGMN_05925 0.73 -1.9 1,104,201 + allC NIAGMN_05925 0.78 -1.0 1,104,201 + allC NIAGMN_05925 0.78 +0.7
Or see this region's nucleotide sequence