Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nagB and nagA are separated by 59 nucleotides nagA and nagC are separated by 8 nucleotides
NIAGMN_05215: nagB - glucosamine-6-phosphate deaminase, at 957,406 to 958,206
nagB
NIAGMN_05220: nagA - N-acetylglucosamine-6-phosphate deacetylase, at 958,266 to 959,414
nagA
NIAGMN_05225: nagC - DNA-binding transcriptional regulator NagC, at 959,423 to 960,643
nagC
Position (kb) 958
959
960 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 957.272 kb on + strand at 957.272 kb on + strand at 957.293 kb on + strand at 957.365 kb on + strand at 957.382 kb on - strand at 957.392 kb on + strand at 957.393 kb on - strand at 957.426 kb on + strand at 957.550 kb on + strand, within nagB at 957.551 kb on - strand, within nagB at 957.574 kb on - strand, within nagB at 957.658 kb on + strand, within nagB at 957.659 kb on - strand, within nagB at 957.659 kb on - strand, within nagB at 957.822 kb on + strand, within nagB at 957.834 kb on + strand, within nagB at 957.834 kb on + strand, within nagB at 957.873 kb on + strand, within nagB at 957.955 kb on + strand, within nagB at 957.978 kb on + strand, within nagB at 957.978 kb on + strand, within nagB at 958.071 kb on + strand, within nagB at 958.071 kb on + strand, within nagB at 958.071 kb on + strand, within nagB at 958.072 kb on - strand, within nagB at 958.204 kb on + strand at 958.269 kb on + strand at 958.269 kb on + strand at 958.269 kb on + strand at 958.541 kb on + strand, within nagA at 958.544 kb on + strand, within nagA at 958.544 kb on + strand, within nagA at 958.544 kb on + strand, within nagA at 958.548 kb on + strand, within nagA at 958.548 kb on + strand, within nagA at 958.562 kb on + strand, within nagA at 958.562 kb on + strand, within nagA at 958.712 kb on + strand, within nagA at 958.898 kb on + strand, within nagA at 958.917 kb on + strand, within nagA at 958.917 kb on + strand, within nagA at 958.932 kb on + strand, within nagA at 958.932 kb on + strand, within nagA at 958.932 kb on + strand, within nagA at 958.932 kb on + strand, within nagA at 958.934 kb on + strand, within nagA at 958.989 kb on + strand, within nagA at 958.989 kb on + strand, within nagA at 958.989 kb on + strand, within nagA at 959.000 kb on + strand, within nagA at 959.045 kb on + strand, within nagA at 959.046 kb on - strand, within nagA at 959.142 kb on + strand, within nagA at 959.142 kb on + strand, within nagA at 959.142 kb on + strand, within nagA at 959.142 kb on + strand, within nagA at 959.142 kb on + strand, within nagA at 959.142 kb on + strand, within nagA at 959.142 kb on + strand, within nagA at 959.142 kb on + strand, within nagA at 959.145 kb on + strand, within nagA at 959.145 kb on + strand, within nagA at 959.219 kb on + strand, within nagA at 959.264 kb on + strand, within nagA at 959.330 kb on + strand at 959.330 kb on + strand at 959.335 kb on + strand at 959.404 kb on + strand at 959.412 kb on + strand at 959.422 kb on + strand at 959.422 kb on + strand at 959.422 kb on + strand at 959.448 kb on + strand at 959.449 kb on - strand at 959.449 kb on - strand at 959.449 kb on - strand at 959.452 kb on + strand at 959.452 kb on + strand at 959.452 kb on + strand at 959.452 kb on + strand at 959.452 kb on + strand at 959.452 kb on + strand at 959.453 kb on - strand at 959.453 kb on - strand at 959.476 kb on + strand at 959.492 kb on + strand at 959.590 kb on + strand, within nagC at 959.650 kb on + strand, within nagC at 959.656 kb on + strand, within nagC at 959.694 kb on + strand, within nagC at 959.840 kb on + strand, within nagC at 959.841 kb on - strand, within nagC at 959.921 kb on + strand, within nagC at 959.921 kb on + strand, within nagC at 959.921 kb on + strand, within nagC at 959.922 kb on - strand, within nagC at 959.932 kb on + strand, within nagC at 959.932 kb on + strand, within nagC at 959.933 kb on - strand, within nagC at 959.958 kb on + strand, within nagC at 960.013 kb on + strand, within nagC at 960.035 kb on + strand, within nagC at 960.035 kb on + strand, within nagC at 960.035 kb on + strand, within nagC at 960.106 kb on + strand, within nagC at 960.163 kb on + strand, within nagC at 960.279 kb on - strand, within nagC at 960.409 kb on + strand, within nagC
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 957,272 + -0.3 957,272 + -1.5 957,293 + +1.7 957,365 + +0.5 957,382 - -1.6 957,392 + -0.2 957,393 - -1.6 957,426 + +1.0 957,550 + nagB NIAGMN_05215 0.18 +1.1 957,551 - nagB NIAGMN_05215 0.18 -1.1 957,574 - nagB NIAGMN_05215 0.21 +0.1 957,658 + nagB NIAGMN_05215 0.31 -2.2 957,659 - nagB NIAGMN_05215 0.32 +0.4 957,659 - nagB NIAGMN_05215 0.32 +3.1 957,822 + nagB NIAGMN_05215 0.52 -0.4 957,834 + nagB NIAGMN_05215 0.53 +2.3 957,834 + nagB NIAGMN_05215 0.53 +1.1 957,873 + nagB NIAGMN_05215 0.58 +0.1 957,955 + nagB NIAGMN_05215 0.69 +0.8 957,978 + nagB NIAGMN_05215 0.71 -3.2 957,978 + nagB NIAGMN_05215 0.71 -0.8 958,071 + nagB NIAGMN_05215 0.83 +0.2 958,071 + nagB NIAGMN_05215 0.83 -0.3 958,071 + nagB NIAGMN_05215 0.83 -1.6 958,072 - nagB NIAGMN_05215 0.83 -1.7 958,204 + +0.9 958,269 + -0.7 958,269 + -2.0 958,269 + +3.2 958,541 + nagA NIAGMN_05220 0.24 -1.4 958,544 + nagA NIAGMN_05220 0.24 -0.3 958,544 + nagA NIAGMN_05220 0.24 -1.0 958,544 + nagA NIAGMN_05220 0.24 +1.3 958,548 + nagA NIAGMN_05220 0.25 -0.4 958,548 + nagA NIAGMN_05220 0.25 +1.4 958,562 + nagA NIAGMN_05220 0.26 -2.0 958,562 + nagA NIAGMN_05220 0.26 -0.1 958,712 + nagA NIAGMN_05220 0.39 +1.8 958,898 + nagA NIAGMN_05220 0.55 -3.2 958,917 + nagA NIAGMN_05220 0.57 -1.5 958,917 + nagA NIAGMN_05220 0.57 -1.4 958,932 + nagA NIAGMN_05220 0.58 -2.0 958,932 + nagA NIAGMN_05220 0.58 -0.3 958,932 + nagA NIAGMN_05220 0.58 -0.1 958,932 + nagA NIAGMN_05220 0.58 -0.8 958,934 + nagA NIAGMN_05220 0.58 -1.0 958,989 + nagA NIAGMN_05220 0.63 -0.3 958,989 + nagA NIAGMN_05220 0.63 -1.9 958,989 + nagA NIAGMN_05220 0.63 -1.4 959,000 + nagA NIAGMN_05220 0.64 +1.2 959,045 + nagA NIAGMN_05220 0.68 -1.3 959,046 - nagA NIAGMN_05220 0.68 +0.6 959,142 + nagA NIAGMN_05220 0.76 -2.2 959,142 + nagA NIAGMN_05220 0.76 -0.1 959,142 + nagA NIAGMN_05220 0.76 -1.8 959,142 + nagA NIAGMN_05220 0.76 -1.3 959,142 + nagA NIAGMN_05220 0.76 -0.6 959,142 + nagA NIAGMN_05220 0.76 -3.1 959,142 + nagA NIAGMN_05220 0.76 +0.1 959,142 + nagA NIAGMN_05220 0.76 -1.8 959,145 + nagA NIAGMN_05220 0.77 -1.6 959,145 + nagA NIAGMN_05220 0.77 -2.4 959,219 + nagA NIAGMN_05220 0.83 -0.6 959,264 + nagA NIAGMN_05220 0.87 -0.1 959,330 + +0.0 959,330 + +1.2 959,335 + -1.8 959,404 + -1.4 959,412 + -0.1 959,422 + +1.0 959,422 + -1.0 959,422 + -1.4 959,448 + -0.5 959,449 - -0.6 959,449 - +1.7 959,449 - +3.2 959,452 + -1.1 959,452 + +0.3 959,452 + +1.2 959,452 + -1.6 959,452 + -1.8 959,452 + -1.3 959,453 - -1.1 959,453 - -2.6 959,476 + -0.9 959,492 + -1.2 959,590 + nagC NIAGMN_05225 0.14 +0.5 959,650 + nagC NIAGMN_05225 0.19 +0.2 959,656 + nagC NIAGMN_05225 0.19 -0.9 959,694 + nagC NIAGMN_05225 0.22 +0.8 959,840 + nagC NIAGMN_05225 0.34 +0.4 959,841 - nagC NIAGMN_05225 0.34 -0.4 959,921 + nagC NIAGMN_05225 0.41 -0.1 959,921 + nagC NIAGMN_05225 0.41 +2.6 959,921 + nagC NIAGMN_05225 0.41 -2.1 959,922 - nagC NIAGMN_05225 0.41 -0.6 959,932 + nagC NIAGMN_05225 0.42 +0.3 959,932 + nagC NIAGMN_05225 0.42 +0.5 959,933 - nagC NIAGMN_05225 0.42 -0.1 959,958 + nagC NIAGMN_05225 0.44 -2.5 960,013 + nagC NIAGMN_05225 0.48 -2.4 960,035 + nagC NIAGMN_05225 0.50 -1.1 960,035 + nagC NIAGMN_05225 0.50 -2.2 960,035 + nagC NIAGMN_05225 0.50 +0.1 960,106 + nagC NIAGMN_05225 0.56 -0.2 960,163 + nagC NIAGMN_05225 0.61 -0.4 960,279 - nagC NIAGMN_05225 0.70 -1.9 960,409 + nagC NIAGMN_05225 0.81 +0.2
Or see this region's nucleotide sequence