Strain Fitness in Escherichia coli ECRC102 around NIAGMN_04445

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntbioF and bioB overlap by 4 nucleotidesbioB and bioA are separated by 86 nucleotides NIAGMN_04440: bioF - 8-amino-7-oxononanoate synthase, at 807,139 to 808,293 bioF NIAGMN_04445: bioB - biotin synthase BioB, at 808,290 to 809,330 bioB NIAGMN_04450: bioA - adenosylmethionine--8-amino-7-oxononanoate transaminase, at 809,417 to 810,706 bioA Position (kb) 808 809 810Strain fitness (log2 ratio) -2 -1 0 1 2at 807.298 kb on + strand, within bioFat 807.361 kb on - strand, within bioFat 807.467 kb on - strand, within bioFat 807.467 kb on - strand, within bioFat 807.493 kb on - strand, within bioFat 807.503 kb on + strand, within bioFat 807.503 kb on + strand, within bioFat 807.504 kb on - strand, within bioFat 807.504 kb on - strand, within bioFat 807.504 kb on - strand, within bioFat 807.504 kb on - strand, within bioFat 807.504 kb on - strand, within bioFat 807.504 kb on - strand, within bioFat 807.547 kb on - strand, within bioFat 807.837 kb on + strand, within bioFat 807.869 kb on - strand, within bioFat 807.979 kb on - strand, within bioFat 807.982 kb on + strand, within bioFat 807.982 kb on + strand, within bioFat 807.983 kb on - strand, within bioFat 807.983 kb on - strand, within bioFat 808.047 kb on + strand, within bioFat 808.048 kb on - strand, within bioFat 808.119 kb on + strand, within bioFat 808.139 kb on - strand, within bioFat 808.148 kb on + strand, within bioFat 808.149 kb on - strand, within bioFat 808.293 kb on + strandat 808.310 kb on - strandat 808.312 kb on + strandat 808.313 kb on - strandat 808.434 kb on - strand, within bioBat 808.559 kb on - strand, within bioBat 808.826 kb on + strand, within bioBat 808.827 kb on - strand, within bioBat 808.827 kb on - strand, within bioBat 808.827 kb on - strand, within bioBat 808.849 kb on + strand, within bioBat 809.155 kb on + strand, within bioBat 809.416 kb on + strandat 809.453 kb on - strandat 809.453 kb on - strandat 809.465 kb on + strandat 809.465 kb on + strandat 809.465 kb on + strandat 809.561 kb on - strand, within bioAat 809.609 kb on - strand, within bioAat 809.858 kb on - strand, within bioAat 810.083 kb on + strand, within bioAat 810.083 kb on + strand, within bioAat 810.133 kb on + strand, within bioAat 810.134 kb on - strand, within bioA

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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807,298 + bioF NIAGMN_04440 0.14 +1.1
807,361 - bioF NIAGMN_04440 0.19 +0.5
807,467 - bioF NIAGMN_04440 0.28 -1.2
807,467 - bioF NIAGMN_04440 0.28 +0.5
807,493 - bioF NIAGMN_04440 0.31 -0.9
807,503 + bioF NIAGMN_04440 0.32 -2.0
807,503 + bioF NIAGMN_04440 0.32 -0.5
807,504 - bioF NIAGMN_04440 0.32 -2.2
807,504 - bioF NIAGMN_04440 0.32 +0.1
807,504 - bioF NIAGMN_04440 0.32 -0.2
807,504 - bioF NIAGMN_04440 0.32 +1.1
807,504 - bioF NIAGMN_04440 0.32 +1.2
807,504 - bioF NIAGMN_04440 0.32 +1.9
807,547 - bioF NIAGMN_04440 0.35 +0.3
807,837 + bioF NIAGMN_04440 0.60 +2.5
807,869 - bioF NIAGMN_04440 0.63 -1.2
807,979 - bioF NIAGMN_04440 0.73 -0.1
807,982 + bioF NIAGMN_04440 0.73 +0.1
807,982 + bioF NIAGMN_04440 0.73 -2.1
807,983 - bioF NIAGMN_04440 0.73 -0.4
807,983 - bioF NIAGMN_04440 0.73 +0.2
808,047 + bioF NIAGMN_04440 0.79 -0.1
808,048 - bioF NIAGMN_04440 0.79 +0.1
808,119 + bioF NIAGMN_04440 0.85 -0.2
808,139 - bioF NIAGMN_04440 0.87 +1.4
808,148 + bioF NIAGMN_04440 0.87 -1.2
808,149 - bioF NIAGMN_04440 0.87 -1.6
808,293 + -1.4
808,310 - +0.1
808,312 + +0.1
808,313 - -1.1
808,434 - bioB NIAGMN_04445 0.14 +1.8
808,559 - bioB NIAGMN_04445 0.26 -0.4
808,826 + bioB NIAGMN_04445 0.51 -1.1
808,827 - bioB NIAGMN_04445 0.52 -1.5
808,827 - bioB NIAGMN_04445 0.52 -0.7
808,827 - bioB NIAGMN_04445 0.52 -2.2
808,849 + bioB NIAGMN_04445 0.54 +0.1
809,155 + bioB NIAGMN_04445 0.83 +0.3
809,416 + -0.6
809,453 - -2.5
809,453 - +1.1
809,465 + +0.6
809,465 + +1.6
809,465 + -0.2
809,561 - bioA NIAGMN_04450 0.11 -1.7
809,609 - bioA NIAGMN_04450 0.15 -0.1
809,858 - bioA NIAGMN_04450 0.34 +2.6
810,083 + bioA NIAGMN_04450 0.52 +1.2
810,083 + bioA NIAGMN_04450 0.52 +0.2
810,133 + bioA NIAGMN_04450 0.56 +1.1
810,134 - bioA NIAGMN_04450 0.56 +0.4

Or see this region's nucleotide sequence