Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt grxA and ybjM are separated by 29 nucleotides ybjM and ybjL are separated by 269 nucleotides ybjL and rcdA are separated by 174 nucleotides rcdA and ybjJ are separated by 83 nucleotides
NIAGMN_04030: grxA - glutaredoxin 1, at 725,000 to 725,257
grxA
NIAGMN_04035: ybjM - Inner membrane protein YbjM, at 725,287 to 725,664
ybjM
NIAGMN_04040: ybjL - aspartate:alanine antiporter, at 725,934 to 727,619
ybjL
NIAGMN_04050: rcdA - DNA-binding transcriptional regulator RcdA, at 727,794 to 728,330
rcdA
NIAGMN_04055: ybjJ - Inner membrane protein YbjJ, at 728,414 to 729,622
ybjJ
Position (kb) 725
726
727
728 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 724.978 kb on + strand at 724.978 kb on + strand at 724.978 kb on + strand at 725.036 kb on + strand, within grxA at 725.036 kb on + strand, within grxA at 725.096 kb on + strand, within grxA at 725.102 kb on + strand, within grxA at 725.102 kb on + strand, within grxA at 725.102 kb on + strand, within grxA at 725.106 kb on + strand, within grxA at 725.106 kb on + strand, within grxA at 725.106 kb on + strand, within grxA at 725.106 kb on + strand, within grxA at 725.110 kb on + strand, within grxA at 725.131 kb on + strand, within grxA at 725.142 kb on + strand, within grxA at 725.142 kb on + strand, within grxA at 725.166 kb on + strand, within grxA at 725.203 kb on + strand, within grxA at 725.329 kb on - strand, within ybjM at 725.375 kb on - strand, within ybjM at 725.377 kb on + strand, within ybjM at 725.378 kb on - strand, within ybjM at 725.433 kb on + strand, within ybjM at 725.433 kb on + strand, within ybjM at 725.434 kb on - strand, within ybjM at 725.434 kb on - strand, within ybjM at 725.687 kb on + strand at 725.749 kb on + strand at 725.755 kb on - strand at 725.755 kb on - strand at 725.772 kb on - strand at 725.774 kb on - strand at 725.774 kb on - strand at 725.810 kb on - strand at 725.811 kb on + strand at 725.812 kb on - strand at 725.823 kb on - strand at 726.082 kb on + strand at 726.211 kb on + strand, within ybjL at 726.211 kb on + strand, within ybjL at 726.262 kb on + strand, within ybjL at 726.262 kb on + strand, within ybjL at 726.263 kb on - strand, within ybjL at 726.266 kb on + strand, within ybjL at 726.307 kb on + strand, within ybjL at 726.318 kb on - strand, within ybjL at 726.432 kb on + strand, within ybjL at 726.436 kb on + strand, within ybjL at 726.436 kb on + strand, within ybjL at 726.436 kb on + strand, within ybjL at 726.882 kb on - strand, within ybjL at 726.926 kb on + strand, within ybjL at 727.255 kb on + strand, within ybjL at 727.255 kb on + strand, within ybjL at 727.289 kb on + strand, within ybjL at 727.289 kb on + strand, within ybjL at 727.419 kb on + strand, within ybjL at 727.419 kb on + strand, within ybjL at 727.419 kb on + strand, within ybjL at 727.423 kb on + strand, within ybjL at 727.548 kb on + strand at 727.548 kb on + strand at 727.549 kb on - strand at 727.600 kb on + strand at 727.688 kb on + strand at 727.793 kb on - strand at 727.793 kb on - strand at 727.796 kb on - strand at 727.796 kb on - strand at 727.848 kb on - strand, within rcdA at 727.884 kb on - strand, within rcdA at 728.054 kb on - strand, within rcdA at 728.189 kb on - strand, within rcdA at 728.291 kb on + strand at 728.435 kb on - strand at 728.435 kb on - strand at 728.525 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 724,978 + -0.8 724,978 + +0.1 724,978 + +0.2 725,036 + grxA NIAGMN_04030 0.14 -1.0 725,036 + grxA NIAGMN_04030 0.14 +2.4 725,096 + grxA NIAGMN_04030 0.37 -0.7 725,102 + grxA NIAGMN_04030 0.40 +0.8 725,102 + grxA NIAGMN_04030 0.40 -0.4 725,102 + grxA NIAGMN_04030 0.40 +2.1 725,106 + grxA NIAGMN_04030 0.41 -2.1 725,106 + grxA NIAGMN_04030 0.41 -1.2 725,106 + grxA NIAGMN_04030 0.41 +0.6 725,106 + grxA NIAGMN_04030 0.41 +0.2 725,110 + grxA NIAGMN_04030 0.43 +0.1 725,131 + grxA NIAGMN_04030 0.51 -2.9 725,142 + grxA NIAGMN_04030 0.55 -0.0 725,142 + grxA NIAGMN_04030 0.55 -0.2 725,166 + grxA NIAGMN_04030 0.64 -0.6 725,203 + grxA NIAGMN_04030 0.79 -0.6 725,329 - ybjM NIAGMN_04035 0.11 -1.1 725,375 - ybjM NIAGMN_04035 0.23 -0.4 725,377 + ybjM NIAGMN_04035 0.24 +2.7 725,378 - ybjM NIAGMN_04035 0.24 -0.1 725,433 + ybjM NIAGMN_04035 0.39 +0.6 725,433 + ybjM NIAGMN_04035 0.39 -0.2 725,434 - ybjM NIAGMN_04035 0.39 +0.8 725,434 - ybjM NIAGMN_04035 0.39 -1.8 725,687 + +0.2 725,749 + -0.2 725,755 - +1.9 725,755 - -2.2 725,772 - +0.2 725,774 - -1.8 725,774 - +0.4 725,810 - -2.5 725,811 + -0.8 725,812 - +0.0 725,823 - +2.4 726,082 + +0.0 726,211 + ybjL NIAGMN_04040 0.16 +1.0 726,211 + ybjL NIAGMN_04040 0.16 -1.1 726,262 + ybjL NIAGMN_04040 0.19 +0.0 726,262 + ybjL NIAGMN_04040 0.19 -1.2 726,263 - ybjL NIAGMN_04040 0.20 +1.1 726,266 + ybjL NIAGMN_04040 0.20 +0.4 726,307 + ybjL NIAGMN_04040 0.22 +0.6 726,318 - ybjL NIAGMN_04040 0.23 +0.5 726,432 + ybjL NIAGMN_04040 0.30 -1.2 726,436 + ybjL NIAGMN_04040 0.30 -1.2 726,436 + ybjL NIAGMN_04040 0.30 +1.0 726,436 + ybjL NIAGMN_04040 0.30 -1.4 726,882 - ybjL NIAGMN_04040 0.56 +0.1 726,926 + ybjL NIAGMN_04040 0.59 +0.2 727,255 + ybjL NIAGMN_04040 0.78 +3.1 727,255 + ybjL NIAGMN_04040 0.78 -0.9 727,289 + ybjL NIAGMN_04040 0.80 -2.3 727,289 + ybjL NIAGMN_04040 0.80 +0.3 727,419 + ybjL NIAGMN_04040 0.88 -0.2 727,419 + ybjL NIAGMN_04040 0.88 -1.0 727,419 + ybjL NIAGMN_04040 0.88 -2.5 727,423 + ybjL NIAGMN_04040 0.88 +0.6 727,548 + +0.6 727,548 + +0.8 727,549 - +1.8 727,600 + +0.9 727,688 + +0.5 727,793 - -0.9 727,793 - +0.5 727,796 - -0.4 727,796 - -0.6 727,848 - rcdA NIAGMN_04050 0.10 +2.2 727,884 - rcdA NIAGMN_04050 0.17 -0.6 728,054 - rcdA NIAGMN_04050 0.48 -2.6 728,189 - rcdA NIAGMN_04050 0.74 +0.4 728,291 + -0.8 728,435 - -1.4 728,435 - -2.4 728,525 - +0.2
Or see this region's nucleotide sequence