Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycbX and zapC overlap by 4 nucleotides zapC and pyrD are separated by 173 nucleotides pyrD and NIAGMN_03020 are separated by 277 nucleotides NIAGMN_03020 and fimA are separated by 13 nucleotides
NIAGMN_03005: ycbX - 6-N-hydroxylaminopurine resistance protein YcbX, at 517,721 to 518,830
ycbX
NIAGMN_03010: zapC - cell division protein ZapC, at 518,827 to 519,369
zapC
NIAGMN_03015: pyrD - quinone-dependent dihydroorotate dehydrogenase, at 519,543 to 520,553
pyrD
NIAGMN_03020: NIAGMN_03020 - PapD-N domain-containing protein, at 520,831 to 521,352
_03020
NIAGMN_03025: fimA - fimbrial protein, at 521,366 to 521,929
fimA
Position (kb) 519
520
521 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2
3
4 at 518.895 kb on + strand, within zapC at 518.959 kb on + strand, within zapC at 519.200 kb on + strand, within zapC at 519.289 kb on - strand, within zapC at 519.429 kb on - strand at 519.461 kb on + strand at 519.514 kb on - strand at 519.519 kb on - strand at 519.816 kb on - strand, within pyrD at 519.853 kb on - strand, within pyrD at 520.023 kb on - strand, within pyrD at 520.183 kb on - strand, within pyrD at 520.413 kb on - strand, within pyrD at 520.532 kb on + strand at 520.546 kb on + strand at 520.549 kb on + strand at 520.549 kb on + strand at 520.742 kb on + strand at 520.798 kb on + strand at 520.818 kb on - strand at 520.842 kb on + strand at 520.842 kb on + strand at 520.843 kb on - strand at 520.845 kb on + strand at 520.845 kb on + strand at 520.954 kb on - strand, within NIAGMN_03020 at 520.979 kb on - strand, within NIAGMN_03020 at 521.024 kb on - strand, within NIAGMN_03020 at 521.239 kb on - strand, within NIAGMN_03020 at 521.268 kb on + strand, within NIAGMN_03020 at 521.364 kb on + strand at 521.364 kb on + strand at 521.365 kb on - strand at 521.373 kb on + strand at 521.373 kb on + strand at 521.381 kb on + strand at 521.382 kb on - strand at 521.474 kb on - strand, within fimA at 521.477 kb on + strand, within fimA at 521.477 kb on + strand, within fimA at 521.478 kb on - strand, within fimA
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 518,895 + zapC NIAGMN_03010 0.13 -2.2 518,959 + zapC NIAGMN_03010 0.24 -0.4 519,200 + zapC NIAGMN_03010 0.69 -0.6 519,289 - zapC NIAGMN_03010 0.85 +2.6 519,429 - +0.2 519,461 + -1.2 519,514 - +0.0 519,519 - +0.2 519,816 - pyrD NIAGMN_03015 0.27 -1.2 519,853 - pyrD NIAGMN_03015 0.31 -1.3 520,023 - pyrD NIAGMN_03015 0.47 -5.8 520,183 - pyrD NIAGMN_03015 0.63 +0.0 520,413 - pyrD NIAGMN_03015 0.86 -1.1 520,532 + -1.0 520,546 + -3.1 520,549 + -3.5 520,549 + -2.9 520,742 + -1.0 520,798 + +0.9 520,818 - -0.0 520,842 + -0.8 520,842 + +0.4 520,843 - +0.6 520,845 + +4.6 520,845 + -2.3 520,954 - NIAGMN_03020 0.24 -0.6 520,979 - NIAGMN_03020 0.28 -0.6 521,024 - NIAGMN_03020 0.37 -1.0 521,239 - NIAGMN_03020 0.78 +1.4 521,268 + NIAGMN_03020 0.84 -0.5 521,364 + +1.0 521,364 + -1.2 521,365 - +2.5 521,373 + -0.4 521,373 + +0.8 521,381 + +0.8 521,382 - -0.4 521,474 - fimA NIAGMN_03025 0.19 +1.1 521,477 + fimA NIAGMN_03025 0.20 -2.8 521,477 + fimA NIAGMN_03025 0.20 -0.7 521,478 - fimA NIAGMN_03025 0.20 +3.9
Or see this region's nucleotide sequence