Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nusB and ribE are separated by 19 nucleotides ribE and ribD are separated by 88 nucleotides ribD and nrdR are separated by 3 nucleotides nrdR and yajI are separated by 150 nucleotides
MCAODC_27670: nusB - transcription antitermination factor NusB, at 2,280,671 to 2,281,090
nusB
MCAODC_27675: ribE - 6,7-dimethyl-8-ribityllumazine synthase, at 2,281,110 to 2,281,580
ribE
MCAODC_27680: ribD - bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD, at 2,281,669 to 2,282,772
ribD
MCAODC_27685: nrdR - transcriptional regulator NrdR, at 2,282,776 to 2,283,225
nrdR
MCAODC_27690: yajI - Uncharacterized lipoprotein YajI, at 2,283,376 to 2,283,915
yajI
Position (kb)
2281
2282
2283 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2280.823 kb on - strand, within nusB at 2281.627 kb on - strand at 2281.635 kb on - strand at 2281.635 kb on - strand at 2282.818 kb on - strand at 2282.818 kb on - strand at 2282.856 kb on - strand, within nrdR at 2282.866 kb on - strand, within nrdR at 2282.953 kb on - strand, within nrdR at 2282.953 kb on - strand, within nrdR at 2282.956 kb on - strand, within nrdR at 2283.124 kb on - strand, within nrdR at 2283.124 kb on - strand, within nrdR at 2283.193 kb on - strand at 2283.226 kb on - strand at 2283.280 kb on - strand at 2283.307 kb on - strand at 2283.333 kb on - strand at 2283.340 kb on - strand at 2283.350 kb on + strand at 2283.375 kb on + strand at 2283.376 kb on - strand at 2283.381 kb on + strand at 2283.381 kb on + strand at 2283.382 kb on - strand at 2283.382 kb on - strand at 2283.384 kb on + strand at 2283.568 kb on + strand, within yajI at 2283.618 kb on + strand, within yajI at 2283.618 kb on + strand, within yajI at 2283.623 kb on + strand, within yajI at 2283.623 kb on + strand, within yajI at 2283.623 kb on + strand, within yajI at 2283.623 kb on + strand, within yajI at 2283.623 kb on + strand, within yajI at 2283.624 kb on - strand, within yajI at 2283.624 kb on - strand, within yajI at 2283.624 kb on - strand, within yajI at 2283.627 kb on + strand, within yajI at 2283.627 kb on + strand, within yajI at 2283.628 kb on - strand, within yajI at 2283.628 kb on - strand, within yajI at 2283.642 kb on + strand, within yajI at 2283.642 kb on + strand, within yajI at 2283.645 kb on + strand, within yajI at 2283.646 kb on - strand, within yajI at 2283.654 kb on + strand, within yajI at 2283.677 kb on + strand, within yajI at 2283.677 kb on + strand, within yajI at 2283.677 kb on + strand, within yajI at 2283.678 kb on - strand, within yajI at 2283.681 kb on + strand, within yajI at 2283.682 kb on - strand, within yajI at 2283.755 kb on + strand, within yajI
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 2,280,823 - nusB MCAODC_27670 0.36 -1.5 2,281,627 - +0.6 2,281,635 - +0.1 2,281,635 - -1.7 2,282,818 - -0.4 2,282,818 - +0.3 2,282,856 - nrdR MCAODC_27685 0.18 -1.2 2,282,866 - nrdR MCAODC_27685 0.20 +0.3 2,282,953 - nrdR MCAODC_27685 0.39 +0.3 2,282,953 - nrdR MCAODC_27685 0.39 -0.7 2,282,956 - nrdR MCAODC_27685 0.40 +0.6 2,283,124 - nrdR MCAODC_27685 0.77 +0.4 2,283,124 - nrdR MCAODC_27685 0.77 -1.1 2,283,193 - +1.0 2,283,226 - +0.4 2,283,280 - +0.6 2,283,307 - +1.4 2,283,333 - +0.1 2,283,340 - +0.4 2,283,350 + +1.9 2,283,375 + +0.7 2,283,376 - -0.3 2,283,381 + +0.3 2,283,381 + +0.0 2,283,382 - -0.4 2,283,382 - +0.1 2,283,384 + +0.3 2,283,568 + yajI MCAODC_27690 0.36 +0.7 2,283,618 + yajI MCAODC_27690 0.45 +0.0 2,283,618 + yajI MCAODC_27690 0.45 +0.8 2,283,623 + yajI MCAODC_27690 0.46 +2.1 2,283,623 + yajI MCAODC_27690 0.46 -1.4 2,283,623 + yajI MCAODC_27690 0.46 -0.5 2,283,623 + yajI MCAODC_27690 0.46 +0.9 2,283,623 + yajI MCAODC_27690 0.46 -0.2 2,283,624 - yajI MCAODC_27690 0.46 +0.1 2,283,624 - yajI MCAODC_27690 0.46 -0.3 2,283,624 - yajI MCAODC_27690 0.46 -0.7 2,283,627 + yajI MCAODC_27690 0.46 -0.6 2,283,627 + yajI MCAODC_27690 0.46 -0.6 2,283,628 - yajI MCAODC_27690 0.47 +1.0 2,283,628 - yajI MCAODC_27690 0.47 +1.3 2,283,642 + yajI MCAODC_27690 0.49 -0.5 2,283,642 + yajI MCAODC_27690 0.49 +0.7 2,283,645 + yajI MCAODC_27690 0.50 +0.3 2,283,646 - yajI MCAODC_27690 0.50 +2.7 2,283,654 + yajI MCAODC_27690 0.51 -2.5 2,283,677 + yajI MCAODC_27690 0.56 -1.1 2,283,677 + yajI MCAODC_27690 0.56 -1.4 2,283,677 + yajI MCAODC_27690 0.56 -0.1 2,283,678 - yajI MCAODC_27690 0.56 -0.2 2,283,681 + yajI MCAODC_27690 0.56 -0.0 2,283,682 - yajI MCAODC_27690 0.57 +1.1 2,283,755 + yajI MCAODC_27690 0.70 -2.1
Or see this region's nucleotide sequence