Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yecN and cmoA are separated by 40 nucleotides cmoA and cmoB overlap by 4 nucleotides cmoB and torZ are separated by 164 nucleotides
MCAODC_19175: yecN - Inner membrane protein YecN, at 683,463 to 683,858
yecN
MCAODC_19180: cmoA - carboxy-S-adenosyl-L-methionine synthase CmoA, at 683,899 to 684,642
cmoA
MCAODC_19185: cmoB - tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB, at 684,639 to 685,610
cmoB
MCAODC_19195: torZ - trimethylamine N-oxide reductase TorZ, at 685,775 to 688,204
torZ
Position (kb) 684
685
686 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 683.687 kb on + strand, within yecN at 683.687 kb on + strand, within yecN at 683.730 kb on + strand, within yecN at 683.730 kb on + strand, within yecN at 683.823 kb on + strand at 683.823 kb on + strand at 683.823 kb on + strand at 683.823 kb on + strand at 683.824 kb on - strand at 683.825 kb on + strand at 683.826 kb on - strand at 683.866 kb on + strand at 683.898 kb on + strand at 684.039 kb on + strand, within cmoA at 684.040 kb on - strand, within cmoA at 684.141 kb on + strand, within cmoA at 684.142 kb on - strand, within cmoA at 684.202 kb on - strand, within cmoA at 684.211 kb on + strand, within cmoA at 684.222 kb on + strand, within cmoA at 684.223 kb on - strand, within cmoA at 684.230 kb on + strand, within cmoA at 684.556 kb on - strand, within cmoA at 684.597 kb on + strand at 684.620 kb on + strand at 684.722 kb on + strand at 684.722 kb on + strand at 685.001 kb on - strand, within cmoB at 685.133 kb on + strand, within cmoB at 685.138 kb on + strand, within cmoB at 685.174 kb on + strand, within cmoB at 685.174 kb on + strand, within cmoB at 685.174 kb on + strand, within cmoB at 685.178 kb on + strand, within cmoB at 685.178 kb on + strand, within cmoB at 685.212 kb on - strand, within cmoB at 685.379 kb on + strand, within cmoB at 685.442 kb on - strand, within cmoB at 685.475 kb on + strand, within cmoB at 685.476 kb on - strand, within cmoB at 685.696 kb on + strand at 685.697 kb on - strand at 685.730 kb on - strand at 685.730 kb on - strand at 685.776 kb on + strand at 685.842 kb on + strand at 685.842 kb on + strand at 685.843 kb on - strand at 685.843 kb on - strand at 685.843 kb on - strand at 685.928 kb on - strand at 685.973 kb on + strand at 685.974 kb on - strand at 685.974 kb on - strand at 686.096 kb on - strand, within torZ at 686.150 kb on - strand, within torZ at 686.266 kb on + strand, within torZ at 686.296 kb on - strand, within torZ at 686.370 kb on + strand, within torZ at 686.371 kb on - strand, within torZ at 686.527 kb on - strand, within torZ
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 683,687 + yecN MCAODC_19175 0.57 -2.5 683,687 + yecN MCAODC_19175 0.57 +0.9 683,730 + yecN MCAODC_19175 0.67 +1.1 683,730 + yecN MCAODC_19175 0.67 -1.0 683,823 + -0.2 683,823 + -3.1 683,823 + +0.2 683,823 + +1.4 683,824 - +0.3 683,825 + -0.1 683,826 - -0.1 683,866 + +0.5 683,898 + -2.7 684,039 + cmoA MCAODC_19180 0.19 +0.5 684,040 - cmoA MCAODC_19180 0.19 +1.4 684,141 + cmoA MCAODC_19180 0.33 +0.5 684,142 - cmoA MCAODC_19180 0.33 -0.3 684,202 - cmoA MCAODC_19180 0.41 +2.0 684,211 + cmoA MCAODC_19180 0.42 +0.5 684,222 + cmoA MCAODC_19180 0.43 +0.6 684,223 - cmoA MCAODC_19180 0.44 +1.7 684,230 + cmoA MCAODC_19180 0.44 -1.4 684,556 - cmoA MCAODC_19180 0.88 +0.1 684,597 + +0.1 684,620 + +0.5 684,722 + -0.6 684,722 + -2.0 685,001 - cmoB MCAODC_19185 0.37 +0.4 685,133 + cmoB MCAODC_19185 0.51 -3.1 685,138 + cmoB MCAODC_19185 0.51 -1.5 685,174 + cmoB MCAODC_19185 0.55 -1.5 685,174 + cmoB MCAODC_19185 0.55 +1.4 685,174 + cmoB MCAODC_19185 0.55 -0.0 685,178 + cmoB MCAODC_19185 0.55 -1.4 685,178 + cmoB MCAODC_19185 0.55 -0.8 685,212 - cmoB MCAODC_19185 0.59 -0.7 685,379 + cmoB MCAODC_19185 0.76 -0.1 685,442 - cmoB MCAODC_19185 0.83 +0.4 685,475 + cmoB MCAODC_19185 0.86 +1.4 685,476 - cmoB MCAODC_19185 0.86 +1.1 685,696 + -1.2 685,697 - +0.3 685,730 - -2.0 685,730 - -0.5 685,776 + +0.7 685,842 + -0.0 685,842 + +1.6 685,843 - +1.3 685,843 - -0.2 685,843 - +0.5 685,928 - +1.4 685,973 + +0.9 685,974 - +0.1 685,974 - +0.8 686,096 - torZ MCAODC_19195 0.13 +1.1 686,150 - torZ MCAODC_19195 0.15 -0.0 686,266 + torZ MCAODC_19195 0.20 +0.9 686,296 - torZ MCAODC_19195 0.21 +0.3 686,370 + torZ MCAODC_19195 0.24 +0.1 686,371 - torZ MCAODC_19195 0.25 -1.8 686,527 - torZ MCAODC_19195 0.31 +3.6
Or see this region's nucleotide sequence