Strain Fitness in Escherichia coli ECRC101 around MCAODC_17590

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttnp-IS3 and tnp-IS3 overlap by 1 nucleotidestnp-IS3 and MCAODC_17590 overlap by 35 nucleotidesMCAODC_17590 and MCAODC_17595 are separated by 87 nucleotidesMCAODC_17595 and MCAODC_17600 are separated by 34 nucleotides MCAODC_17580: tnp-IS3 - IS3 family transposase, at 380,457 to 380,783 tnp-IS3 MCAODC_17585: tnp-IS3 - IS3 family transposase, at 380,783 to 381,670 tnp-IS3 MCAODC_17590: MCAODC_17590 - exodeoxyribonuclease VIII, at 381,636 to 383,105 _17590 MCAODC_17595: MCAODC_17595 - DNA-binding protein, at 383,193 to 383,429 _17595 MCAODC_17600: MCAODC_17600 - Integrase, cryptic prophage CP-933P, at 383,464 to 383,619 _17600 Position (kb) 381 382 383 384Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 381.805 kb on - strand, within MCAODC_17590at 381.805 kb on - strand, within MCAODC_17590at 382.353 kb on + strand, within MCAODC_17590at 382.422 kb on - strand, within MCAODC_17590at 382.608 kb on - strand, within MCAODC_17590at 383.103 kb on + strandat 383.103 kb on + strandat 383.103 kb on + strandat 383.167 kb on + strandat 383.188 kb on + strandat 383.309 kb on + strand, within MCAODC_17595at 383.309 kb on + strand, within MCAODC_17595at 383.309 kb on + strand, within MCAODC_17595at 383.309 kb on + strand, within MCAODC_17595at 383.309 kb on + strand, within MCAODC_17595at 383.310 kb on - strand, within MCAODC_17595at 383.509 kb on - strand, within MCAODC_17600at 383.521 kb on + strand, within MCAODC_17600at 383.523 kb on + strand, within MCAODC_17600at 383.638 kb on - strandat 383.698 kb on + strandat 383.699 kb on - strandat 383.901 kb on + strandat 383.938 kb on + strandat 383.946 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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381,805 - MCAODC_17590 0.11 -0.5
381,805 - MCAODC_17590 0.11 +1.0
382,353 + MCAODC_17590 0.49 -0.3
382,422 - MCAODC_17590 0.53 +0.4
382,608 - MCAODC_17590 0.66 -0.2
383,103 + +0.6
383,103 + -0.2
383,103 + -1.6
383,167 + -0.7
383,188 + -1.4
383,309 + MCAODC_17595 0.49 +0.8
383,309 + MCAODC_17595 0.49 -1.3
383,309 + MCAODC_17595 0.49 +1.8
383,309 + MCAODC_17595 0.49 +1.8
383,309 + MCAODC_17595 0.49 +0.4
383,310 - MCAODC_17595 0.49 +0.3
383,509 - MCAODC_17600 0.29 -0.0
383,521 + MCAODC_17600 0.37 -3.8
383,523 + MCAODC_17600 0.38 -0.7
383,638 - -0.4
383,698 + -1.0
383,699 - +0.6
383,901 + -0.4
383,938 + +0.1
383,946 + +0.7

Or see this region's nucleotide sequence