Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pdxI and MCAODC_16175 are separated by 63 nucleotides MCAODC_16175 and ynbA are separated by 170 nucleotides ynbA and ynbB overlap by 1 nucleotides
MCAODC_16170: pdxI - pyridoxine 4-dehydrogenase, at 127,616 to 128,476
pdxI
MCAODC_16175: MCAODC_16175 - DUF2773 domain-containing protein, at 128,540 to 130,846
_16175
MCAODC_16180: ynbA - Inner membrane protein YnbA, at 131,017 to 131,622
ynbA
MCAODC_16185: ynbB - Uncharacterized protein YnbB, at 131,622 to 132,518
ynbB
Position (kb) 128
129
130
131 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 127.553 kb on - strand at 127.557 kb on - strand at 127.573 kb on - strand at 127.575 kb on - strand at 127.601 kb on - strand at 127.627 kb on + strand at 127.634 kb on - strand at 127.634 kb on - strand at 127.791 kb on - strand, within pdxI at 127.791 kb on - strand, within pdxI at 128.169 kb on - strand, within pdxI at 128.343 kb on + strand, within pdxI at 128.360 kb on - strand, within pdxI at 128.449 kb on + strand at 128.461 kb on + strand at 128.481 kb on + strand at 128.540 kb on - strand at 128.540 kb on - strand at 128.575 kb on - strand at 128.775 kb on + strand, within MCAODC_16175 at 128.787 kb on + strand, within MCAODC_16175 at 128.972 kb on + strand, within MCAODC_16175 at 129.458 kb on + strand, within MCAODC_16175 at 129.459 kb on - strand, within MCAODC_16175 at 129.461 kb on - strand, within MCAODC_16175 at 129.579 kb on + strand, within MCAODC_16175 at 129.920 kb on + strand, within MCAODC_16175 at 129.955 kb on - strand, within MCAODC_16175 at 129.990 kb on + strand, within MCAODC_16175 at 130.296 kb on + strand, within MCAODC_16175 at 130.354 kb on + strand, within MCAODC_16175 at 130.527 kb on + strand, within MCAODC_16175 at 130.527 kb on + strand, within MCAODC_16175 at 130.528 kb on - strand, within MCAODC_16175 at 130.551 kb on + strand, within MCAODC_16175 at 130.678 kb on + strand at 130.701 kb on + strand at 130.767 kb on + strand at 130.848 kb on + strand at 131.083 kb on - strand, within ynbA at 131.137 kb on - strand, within ynbA at 131.440 kb on + strand, within ynbA at 131.509 kb on + strand, within ynbA at 131.731 kb on + strand, within ynbB
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 127,553 - +0.5 127,557 - +1.6 127,573 - +1.5 127,575 - -0.7 127,601 - -0.4 127,627 + -3.2 127,634 - -0.2 127,634 - -0.4 127,791 - pdxI MCAODC_16170 0.20 -2.8 127,791 - pdxI MCAODC_16170 0.20 +0.8 128,169 - pdxI MCAODC_16170 0.64 +1.0 128,343 + pdxI MCAODC_16170 0.84 +0.8 128,360 - pdxI MCAODC_16170 0.86 +0.5 128,449 + -0.2 128,461 + -1.0 128,481 + -0.4 128,540 - -0.6 128,540 - +1.0 128,575 - -1.9 128,775 + MCAODC_16175 0.10 +0.8 128,787 + MCAODC_16175 0.11 -2.6 128,972 + MCAODC_16175 0.19 +1.3 129,458 + MCAODC_16175 0.40 +1.1 129,459 - MCAODC_16175 0.40 -0.1 129,461 - MCAODC_16175 0.40 +0.6 129,579 + MCAODC_16175 0.45 +2.0 129,920 + MCAODC_16175 0.60 -0.7 129,955 - MCAODC_16175 0.61 +0.9 129,990 + MCAODC_16175 0.63 +1.4 130,296 + MCAODC_16175 0.76 -1.8 130,354 + MCAODC_16175 0.79 +1.2 130,527 + MCAODC_16175 0.86 -0.3 130,527 + MCAODC_16175 0.86 +0.4 130,528 - MCAODC_16175 0.86 +0.9 130,551 + MCAODC_16175 0.87 -0.2 130,678 + +1.0 130,701 + -0.7 130,767 + +1.4 130,848 + -2.6 131,083 - ynbA MCAODC_16180 0.11 +0.9 131,137 - ynbA MCAODC_16180 0.20 -0.7 131,440 + ynbA MCAODC_16180 0.70 -1.0 131,509 + ynbA MCAODC_16180 0.81 +1.6 131,731 + ynbB MCAODC_16185 0.12 +1.8
Or see this region's nucleotide sequence