Strain Fitness in Escherichia coli ECRC101 around MCAODC_15610

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttrpD and trpE overlap by 1 nucleotidestrpE and trpL are separated by 91 nucleotidestrpL and yciV are separated by 137 nucleotides MCAODC_15605: trpD - bifunctional anthranilate synthase glutamate amidotransferase component TrpG/anthranilate phosphoribosyltransferase TrpD, at 10,796 to 12,391 trpD MCAODC_15610: trpE - anthranilate synthase component I, at 12,391 to 13,953 trpE MCAODC_15615: trpL - trp operon leader peptide, at 14,045 to 14,089 trpL MCAODC_15620: yciV - 5'-3' exoribonuclease, at 14,227 to 15,108 yciV Position (kb) 12 13 14Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3at 11.410 kb on + strand, within trpDat 11.715 kb on - strand, within trpDat 11.978 kb on - strand, within trpDat 12.102 kb on + strand, within trpDat 12.103 kb on - strand, within trpDat 12.154 kb on + strand, within trpDat 12.154 kb on + strand, within trpDat 12.193 kb on + strand, within trpDat 12.193 kb on + strand, within trpDat 12.194 kb on - strand, within trpDat 12.194 kb on - strand, within trpDat 12.194 kb on - strand, within trpDat 12.273 kb on - strandat 12.313 kb on + strandat 12.349 kb on - strandat 12.353 kb on - strandat 12.365 kb on - strandat 12.392 kb on + strandat 12.392 kb on + strandat 12.392 kb on + strandat 12.426 kb on + strandat 12.590 kb on + strand, within trpEat 12.591 kb on - strand, within trpEat 12.595 kb on + strand, within trpEat 12.596 kb on - strand, within trpEat 12.658 kb on + strand, within trpEat 12.770 kb on + strand, within trpEat 12.770 kb on + strand, within trpEat 12.771 kb on - strand, within trpEat 12.886 kb on - strand, within trpEat 12.984 kb on - strand, within trpEat 13.013 kb on + strand, within trpEat 13.014 kb on - strand, within trpEat 13.079 kb on + strand, within trpEat 13.080 kb on - strand, within trpEat 13.109 kb on + strand, within trpEat 13.110 kb on - strand, within trpEat 13.113 kb on - strand, within trpEat 13.449 kb on + strand, within trpEat 13.500 kb on - strand, within trpEat 13.600 kb on + strand, within trpEat 13.600 kb on + strand, within trpEat 13.664 kb on - strand, within trpEat 13.790 kb on - strand, within trpEat 13.807 kb on + strandat 13.808 kb on - strandat 13.958 kb on - strandat 14.243 kb on - strandat 14.271 kb on + strandat 14.499 kb on + strand, within yciVat 14.499 kb on + strand, within yciVat 14.592 kb on + strand, within yciVat 14.593 kb on - strand, within yciVat 14.593 kb on - strand, within yciVat 14.593 kb on - strand, within yciVat 14.593 kb on - strand, within yciVat 14.594 kb on + strand, within yciVat 14.595 kb on - strand, within yciVat 14.765 kb on + strand, within yciVat 14.775 kb on + strand, within yciVat 14.775 kb on + strand, within yciVat 14.775 kb on + strand, within yciVat 14.813 kb on + strand, within yciVat 14.934 kb on + strand, within yciV

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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11,410 + trpD MCAODC_15605 0.38 -4.6
11,715 - trpD MCAODC_15605 0.58 -3.4
11,978 - trpD MCAODC_15605 0.74 -2.9
12,102 + trpD MCAODC_15605 0.82 -1.2
12,103 - trpD MCAODC_15605 0.82 -4.5
12,154 + trpD MCAODC_15605 0.85 -4.6
12,154 + trpD MCAODC_15605 0.85 -3.7
12,193 + trpD MCAODC_15605 0.88 -3.4
12,193 + trpD MCAODC_15605 0.88 -2.9
12,194 - trpD MCAODC_15605 0.88 -3.0
12,194 - trpD MCAODC_15605 0.88 -2.1
12,194 - trpD MCAODC_15605 0.88 -5.1
12,273 - -2.5
12,313 + -3.4
12,349 - -2.6
12,353 - -1.3
12,365 - -0.3
12,392 + -1.5
12,392 + -5.3
12,392 + -3.6
12,426 + -3.3
12,590 + trpE MCAODC_15610 0.13 -0.8
12,591 - trpE MCAODC_15610 0.13 -2.8
12,595 + trpE MCAODC_15610 0.13 -1.5
12,596 - trpE MCAODC_15610 0.13 -2.0
12,658 + trpE MCAODC_15610 0.17 -3.1
12,770 + trpE MCAODC_15610 0.24 -0.8
12,770 + trpE MCAODC_15610 0.24 -3.4
12,771 - trpE MCAODC_15610 0.24 -0.8
12,886 - trpE MCAODC_15610 0.32 -3.2
12,984 - trpE MCAODC_15610 0.38 -4.8
13,013 + trpE MCAODC_15610 0.40 -1.1
13,014 - trpE MCAODC_15610 0.40 -3.0
13,079 + trpE MCAODC_15610 0.44 -4.0
13,080 - trpE MCAODC_15610 0.44 -3.8
13,109 + trpE MCAODC_15610 0.46 -4.7
13,110 - trpE MCAODC_15610 0.46 -5.6
13,113 - trpE MCAODC_15610 0.46 -0.9
13,449 + trpE MCAODC_15610 0.68 -5.5
13,500 - trpE MCAODC_15610 0.71 -4.2
13,600 + trpE MCAODC_15610 0.77 -2.2
13,600 + trpE MCAODC_15610 0.77 -3.9
13,664 - trpE MCAODC_15610 0.81 -1.6
13,790 - trpE MCAODC_15610 0.90 -1.7
13,807 + -3.7
13,808 - -1.9
13,958 - -0.6
14,243 - -0.7
14,271 + +0.1
14,499 + yciV MCAODC_15620 0.31 -0.9
14,499 + yciV MCAODC_15620 0.31 -0.7
14,592 + yciV MCAODC_15620 0.41 +0.2
14,593 - yciV MCAODC_15620 0.41 +0.4
14,593 - yciV MCAODC_15620 0.41 -0.8
14,593 - yciV MCAODC_15620 0.41 -0.8
14,593 - yciV MCAODC_15620 0.41 +3.0
14,594 + yciV MCAODC_15620 0.42 -0.3
14,595 - yciV MCAODC_15620 0.42 +0.3
14,765 + yciV MCAODC_15620 0.61 +0.9
14,775 + yciV MCAODC_15620 0.62 -1.2
14,775 + yciV MCAODC_15620 0.62 +0.3
14,775 + yciV MCAODC_15620 0.62 +1.6
14,813 + yciV MCAODC_15620 0.66 -0.4
14,934 + yciV MCAODC_15620 0.80 +1.5

Or see this region's nucleotide sequence