Strain Fitness in Escherichia coli ECRC101 around MCAODC_15600

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttrpB and trpCF are separated by 11 nucleotidestrpCF and trpD are separated by 3 nucleotides MCAODC_15595: trpB - tryptophan synthase subunit beta, at 8,229 to 9,422 trpB MCAODC_15600: trpCF - bifunctional indole-3-glycerol-phosphate synthase TrpC/phosphoribosylanthranilate isomerase TrpF, at 9,434 to 10,792 trpCF MCAODC_15605: trpD - bifunctional anthranilate synthase glutamate amidotransferase component TrpG/anthranilate phosphoribosyltransferase TrpD, at 10,796 to 12,391 trpD Position (kb) 9 10 11Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 8.475 kb on - strand, within trpBat 8.531 kb on - strand, within trpBat 8.587 kb on + strand, within trpBat 8.587 kb on + strand, within trpBat 8.864 kb on + strand, within trpBat 8.865 kb on - strand, within trpBat 8.866 kb on + strand, within trpBat 8.866 kb on + strand, within trpBat 9.025 kb on + strand, within trpBat 9.025 kb on + strand, within trpBat 9.025 kb on + strand, within trpBat 9.025 kb on + strand, within trpBat 9.116 kb on + strand, within trpBat 9.135 kb on + strand, within trpBat 9.376 kb on + strandat 9.377 kb on - strandat 9.377 kb on - strandat 9.401 kb on - strandat 9.407 kb on + strandat 9.411 kb on + strandat 9.412 kb on - strandat 9.412 kb on - strandat 9.439 kb on - strandat 9.503 kb on + strandat 9.612 kb on - strand, within trpCFat 9.612 kb on - strand, within trpCFat 9.660 kb on - strand, within trpCFat 9.667 kb on - strand, within trpCFat 9.667 kb on - strand, within trpCFat 9.671 kb on - strand, within trpCFat 9.717 kb on - strand, within trpCFat 9.717 kb on - strand, within trpCFat 9.874 kb on - strand, within trpCFat 10.017 kb on - strand, within trpCFat 10.017 kb on - strand, within trpCFat 10.017 kb on - strand, within trpCFat 10.017 kb on - strand, within trpCFat 10.134 kb on + strand, within trpCFat 10.135 kb on - strand, within trpCFat 10.139 kb on - strand, within trpCFat 10.139 kb on - strand, within trpCFat 10.202 kb on - strand, within trpCFat 10.388 kb on + strand, within trpCFat 10.389 kb on - strand, within trpCFat 10.422 kb on + strand, within trpCFat 10.423 kb on - strand, within trpCFat 10.423 kb on - strand, within trpCFat 10.454 kb on - strand, within trpCFat 10.454 kb on - strand, within trpCFat 10.555 kb on - strand, within trpCFat 10.643 kb on - strand, within trpCFat 10.831 kb on - strandat 11.064 kb on + strand, within trpDat 11.065 kb on - strand, within trpDat 11.072 kb on - strand, within trpDat 11.114 kb on + strand, within trpDat 11.164 kb on - strand, within trpDat 11.213 kb on - strand, within trpDat 11.213 kb on - strand, within trpDat 11.226 kb on - strand, within trpDat 11.357 kb on + strand, within trpDat 11.357 kb on + strand, within trpDat 11.357 kb on + strand, within trpDat 11.358 kb on - strand, within trpDat 11.358 kb on - strand, within trpDat 11.358 kb on - strand, within trpDat 11.363 kb on + strand, within trpDat 11.363 kb on + strand, within trpDat 11.390 kb on - strand, within trpDat 11.390 kb on - strand, within trpDat 11.410 kb on + strand, within trpDat 11.715 kb on - strand, within trpD

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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8,475 - trpB MCAODC_15595 0.21 -4.4
8,531 - trpB MCAODC_15595 0.25 -3.5
8,587 + trpB MCAODC_15595 0.30 -1.3
8,587 + trpB MCAODC_15595 0.30 -2.2
8,864 + trpB MCAODC_15595 0.53 -4.5
8,865 - trpB MCAODC_15595 0.53 -1.7
8,866 + trpB MCAODC_15595 0.53 -4.4
8,866 + trpB MCAODC_15595 0.53 -3.2
9,025 + trpB MCAODC_15595 0.67 -2.7
9,025 + trpB MCAODC_15595 0.67 -1.2
9,025 + trpB MCAODC_15595 0.67 -2.2
9,025 + trpB MCAODC_15595 0.67 -1.3
9,116 + trpB MCAODC_15595 0.74 -1.7
9,135 + trpB MCAODC_15595 0.76 -0.8
9,376 + -2.9
9,377 - -4.3
9,377 - -0.0
9,401 - -0.8
9,407 + -1.2
9,411 + -1.6
9,412 - -2.5
9,412 - -1.4
9,439 - -0.3
9,503 + -5.0
9,612 - trpCF MCAODC_15600 0.13 -3.3
9,612 - trpCF MCAODC_15600 0.13 -3.4
9,660 - trpCF MCAODC_15600 0.17 -5.4
9,667 - trpCF MCAODC_15600 0.17 -3.2
9,667 - trpCF MCAODC_15600 0.17 -3.0
9,671 - trpCF MCAODC_15600 0.17 -3.7
9,717 - trpCF MCAODC_15600 0.21 -1.0
9,717 - trpCF MCAODC_15600 0.21 -1.4
9,874 - trpCF MCAODC_15600 0.32 -2.1
10,017 - trpCF MCAODC_15600 0.43 -2.9
10,017 - trpCF MCAODC_15600 0.43 -1.6
10,017 - trpCF MCAODC_15600 0.43 -1.7
10,017 - trpCF MCAODC_15600 0.43 -3.0
10,134 + trpCF MCAODC_15600 0.52 -4.2
10,135 - trpCF MCAODC_15600 0.52 -3.9
10,139 - trpCF MCAODC_15600 0.52 -1.9
10,139 - trpCF MCAODC_15600 0.52 -2.2
10,202 - trpCF MCAODC_15600 0.57 -0.8
10,388 + trpCF MCAODC_15600 0.70 -1.6
10,389 - trpCF MCAODC_15600 0.70 -2.5
10,422 + trpCF MCAODC_15600 0.73 -5.6
10,423 - trpCF MCAODC_15600 0.73 -4.3
10,423 - trpCF MCAODC_15600 0.73 -2.2
10,454 - trpCF MCAODC_15600 0.75 -5.9
10,454 - trpCF MCAODC_15600 0.75 -2.6
10,555 - trpCF MCAODC_15600 0.82 -3.2
10,643 - trpCF MCAODC_15600 0.89 -1.5
10,831 - -3.5
11,064 + trpD MCAODC_15605 0.17 -1.3
11,065 - trpD MCAODC_15605 0.17 -3.8
11,072 - trpD MCAODC_15605 0.17 -2.8
11,114 + trpD MCAODC_15605 0.20 -2.5
11,164 - trpD MCAODC_15605 0.23 -3.4
11,213 - trpD MCAODC_15605 0.26 -3.2
11,213 - trpD MCAODC_15605 0.26 -1.2
11,226 - trpD MCAODC_15605 0.27 -2.7
11,357 + trpD MCAODC_15605 0.35 -2.5
11,357 + trpD MCAODC_15605 0.35 -2.3
11,357 + trpD MCAODC_15605 0.35 -2.4
11,358 - trpD MCAODC_15605 0.35 -2.9
11,358 - trpD MCAODC_15605 0.35 -1.9
11,358 - trpD MCAODC_15605 0.35 -1.7
11,363 + trpD MCAODC_15605 0.36 -0.8
11,363 + trpD MCAODC_15605 0.36 -4.2
11,390 - trpD MCAODC_15605 0.37 -1.8
11,390 - trpD MCAODC_15605 0.37 -2.6
11,410 + trpD MCAODC_15605 0.38 -4.6
11,715 - trpD MCAODC_15605 0.58 -3.4

Or see this region's nucleotide sequence