Strain Fitness in Escherichia coli ECRC101 around MCAODC_11030

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrpoS and hosA are separated by 119 nucleotideshosA and ecdB are separated by 146 nucleotidesecdB and edcC overlap by 1 nucleotides MCAODC_11020: rpoS - RNA polymerase sigma factor RpoS, at 2,196,675 to 2,197,667 rpoS MCAODC_11025: hosA - Transcriptional regulator HosA, at 2,197,787 to 2,198,194 hosA MCAODC_11030: ecdB - putative UbiX-like flavin prenyltransferase, at 2,198,341 to 2,198,934 ecdB MCAODC_11035: edcC - Phenolic acid decarboxylase, at 2,198,934 to 2,200,361 edcC Position (kb) 2198 2199Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2197.418 kb on + strand, within rpoSat 2197.436 kb on + strand, within rpoSat 2197.436 kb on + strand, within rpoSat 2197.436 kb on + strand, within rpoSat 2197.437 kb on - strand, within rpoSat 2197.437 kb on - strand, within rpoSat 2197.437 kb on - strand, within rpoSat 2197.493 kb on - strand, within rpoSat 2197.546 kb on + strand, within rpoSat 2197.546 kb on + strand, within rpoSat 2197.546 kb on + strand, within rpoSat 2197.546 kb on + strand, within rpoSat 2197.546 kb on + strand, within rpoSat 2197.546 kb on + strand, within rpoSat 2197.546 kb on + strand, within rpoSat 2197.547 kb on - strand, within rpoSat 2197.665 kb on + strandat 2197.666 kb on - strandat 2197.669 kb on + strandat 2197.669 kb on + strandat 2197.669 kb on + strandat 2197.669 kb on + strandat 2197.669 kb on + strandat 2197.669 kb on + strandat 2197.669 kb on + strandat 2197.670 kb on - strandat 2197.670 kb on - strandat 2197.670 kb on - strandat 2197.670 kb on - strandat 2197.788 kb on + strandat 2197.788 kb on + strandat 2197.789 kb on - strandat 2197.789 kb on - strandat 2197.789 kb on - strandat 2197.901 kb on + strand, within hosAat 2198.040 kb on - strand, within hosAat 2198.040 kb on - strand, within hosAat 2198.040 kb on - strand, within hosAat 2198.069 kb on - strand, within hosAat 2198.117 kb on + strand, within hosAat 2198.118 kb on - strand, within hosAat 2198.196 kb on + strandat 2198.213 kb on - strandat 2198.213 kb on - strandat 2198.295 kb on + strandat 2198.296 kb on - strandat 2198.300 kb on + strandat 2198.335 kb on + strandat 2198.335 kb on + strandat 2198.336 kb on - strandat 2198.336 kb on - strandat 2198.336 kb on - strandat 2198.396 kb on + strandat 2198.602 kb on - strand, within ecdBat 2198.649 kb on - strand, within ecdBat 2198.673 kb on + strand, within ecdBat 2198.674 kb on - strand, within ecdBat 2198.718 kb on + strand, within ecdBat 2198.739 kb on + strand, within ecdBat 2198.794 kb on + strand, within ecdBat 2198.794 kb on + strand, within ecdBat 2198.794 kb on + strand, within ecdBat 2198.794 kb on + strand, within ecdBat 2198.794 kb on + strand, within ecdBat 2198.795 kb on - strand, within ecdBat 2198.796 kb on + strand, within ecdBat 2198.813 kb on + strand, within ecdBat 2198.814 kb on - strand, within ecdBat 2198.814 kb on - strand, within ecdBat 2198.950 kb on + strandat 2198.951 kb on - strandat 2198.963 kb on - strandat 2198.963 kb on - strandat 2198.963 kb on - strandat 2199.089 kb on + strand, within edcCat 2199.105 kb on - strand, within edcCat 2199.105 kb on - strand, within edcCat 2199.129 kb on - strand, within edcCat 2199.296 kb on + strand, within edcCat 2199.297 kb on - strand, within edcCat 2199.352 kb on - strand, within edcCat 2199.352 kb on - strand, within edcCat 2199.427 kb on - strand, within edcCat 2199.427 kb on - strand, within edcCat 2199.427 kb on - strand, within edcCat 2199.452 kb on + strand, within edcCat 2199.503 kb on + strand, within edcCat 2199.768 kb on + strand, within edcCat 2199.769 kb on - strand, within edcCat 2199.782 kb on + strand, within edcCat 2199.783 kb on - strand, within edcCat 2199.807 kb on - strand, within edcCat 2199.817 kb on - strand, within edcCat 2199.846 kb on + strand, within edcCat 2199.910 kb on - strand, within edcC

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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2,197,418 + rpoS MCAODC_11020 0.75 +2.0
2,197,436 + rpoS MCAODC_11020 0.77 +2.1
2,197,436 + rpoS MCAODC_11020 0.77 +2.7
2,197,436 + rpoS MCAODC_11020 0.77 +2.3
2,197,437 - rpoS MCAODC_11020 0.77 -1.0
2,197,437 - rpoS MCAODC_11020 0.77 +3.0
2,197,437 - rpoS MCAODC_11020 0.77 +2.1
2,197,493 - rpoS MCAODC_11020 0.82 +2.1
2,197,546 + rpoS MCAODC_11020 0.88 +2.8
2,197,546 + rpoS MCAODC_11020 0.88 +2.2
2,197,546 + rpoS MCAODC_11020 0.88 +2.5
2,197,546 + rpoS MCAODC_11020 0.88 +1.9
2,197,546 + rpoS MCAODC_11020 0.88 +2.4
2,197,546 + rpoS MCAODC_11020 0.88 +1.7
2,197,546 + rpoS MCAODC_11020 0.88 +2.4
2,197,547 - rpoS MCAODC_11020 0.88 +2.5
2,197,665 + +1.3
2,197,666 - -0.5
2,197,669 + -0.0
2,197,669 + +1.6
2,197,669 + +2.0
2,197,669 + +1.4
2,197,669 + +2.4
2,197,669 + +1.5
2,197,669 + +1.4
2,197,670 - +2.4
2,197,670 - +1.5
2,197,670 - +1.3
2,197,670 - +1.4
2,197,788 + +0.5
2,197,788 + -0.5
2,197,789 - -0.6
2,197,789 - -1.3
2,197,789 - +0.3
2,197,901 + hosA MCAODC_11025 0.28 +0.5
2,198,040 - hosA MCAODC_11025 0.62 -0.8
2,198,040 - hosA MCAODC_11025 0.62 -1.0
2,198,040 - hosA MCAODC_11025 0.62 +0.4
2,198,069 - hosA MCAODC_11025 0.69 -1.3
2,198,117 + hosA MCAODC_11025 0.81 -0.0
2,198,118 - hosA MCAODC_11025 0.81 -0.3
2,198,196 + -0.3
2,198,213 - -2.0
2,198,213 - -0.2
2,198,295 + -0.5
2,198,296 - -0.2
2,198,300 + +0.0
2,198,335 + -0.1
2,198,335 + +0.2
2,198,336 - +0.7
2,198,336 - -0.0
2,198,336 - +0.9
2,198,396 + -3.1
2,198,602 - ecdB MCAODC_11030 0.44 -2.3
2,198,649 - ecdB MCAODC_11030 0.52 -0.0
2,198,673 + ecdB MCAODC_11030 0.56 +0.3
2,198,674 - ecdB MCAODC_11030 0.56 +0.0
2,198,718 + ecdB MCAODC_11030 0.63 +0.7
2,198,739 + ecdB MCAODC_11030 0.67 +0.1
2,198,794 + ecdB MCAODC_11030 0.76 -0.3
2,198,794 + ecdB MCAODC_11030 0.76 +0.4
2,198,794 + ecdB MCAODC_11030 0.76 +3.1
2,198,794 + ecdB MCAODC_11030 0.76 -0.6
2,198,794 + ecdB MCAODC_11030 0.76 -0.1
2,198,795 - ecdB MCAODC_11030 0.76 -1.5
2,198,796 + ecdB MCAODC_11030 0.77 +0.6
2,198,813 + ecdB MCAODC_11030 0.79 +0.3
2,198,814 - ecdB MCAODC_11030 0.80 +0.3
2,198,814 - ecdB MCAODC_11030 0.80 -0.1
2,198,950 + -0.6
2,198,951 - -1.1
2,198,963 - -0.1
2,198,963 - +0.6
2,198,963 - +1.0
2,199,089 + edcC MCAODC_11035 0.11 -1.6
2,199,105 - edcC MCAODC_11035 0.12 +1.6
2,199,105 - edcC MCAODC_11035 0.12 -0.2
2,199,129 - edcC MCAODC_11035 0.14 +0.5
2,199,296 + edcC MCAODC_11035 0.25 +0.1
2,199,297 - edcC MCAODC_11035 0.25 +1.4
2,199,352 - edcC MCAODC_11035 0.29 +0.6
2,199,352 - edcC MCAODC_11035 0.29 -1.3
2,199,427 - edcC MCAODC_11035 0.35 +0.9
2,199,427 - edcC MCAODC_11035 0.35 +0.5
2,199,427 - edcC MCAODC_11035 0.35 +0.2
2,199,452 + edcC MCAODC_11035 0.36 +0.4
2,199,503 + edcC MCAODC_11035 0.40 +0.7
2,199,768 + edcC MCAODC_11035 0.58 +1.1
2,199,769 - edcC MCAODC_11035 0.58 -1.0
2,199,782 + edcC MCAODC_11035 0.59 +0.5
2,199,783 - edcC MCAODC_11035 0.59 -0.2
2,199,807 - edcC MCAODC_11035 0.61 +1.2
2,199,817 - edcC MCAODC_11035 0.62 -0.2
2,199,846 + edcC MCAODC_11035 0.64 -0.1
2,199,910 - edcC MCAODC_11035 0.68 -0.4

Or see this region's nucleotide sequence