Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ygfZ and sdhE are separated by 242 nucleotides sdhE and ygfX overlap by 20 nucleotides ygfX and fldB are separated by 39 nucleotides fldB and xerD are separated by 111 nucleotides
MCAODC_10070: ygfZ - tRNA-modifying protein YgfZ, at 2,004,355 to 2,005,335
ygfZ
MCAODC_10075: sdhE - FAD assembly factor SdhE, at 2,005,578 to 2,005,844
sdhE
MCAODC_10080: ygfX - Inner membrane protein YgfX, at 2,005,825 to 2,006,232
ygfX
MCAODC_10085: fldB - flavodoxin FldB, at 2,006,272 to 2,006,793
fldB
MCAODC_10090: xerD - site-specific tyrosine recombinase XerD, at 2,006,905 to 2,007,801
xerD
Position (kb)
2006
2007 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2005.339 kb on - strand at 2005.339 kb on - strand at 2005.364 kb on - strand at 2005.367 kb on - strand at 2005.370 kb on - strand at 2005.370 kb on - strand at 2005.370 kb on - strand at 2005.370 kb on - strand at 2005.374 kb on - strand at 2005.374 kb on - strand at 2005.374 kb on - strand at 2005.380 kb on - strand at 2005.388 kb on + strand at 2005.388 kb on + strand at 2005.388 kb on + strand at 2005.389 kb on - strand at 2005.502 kb on - strand at 2005.507 kb on + strand at 2005.507 kb on + strand at 2005.507 kb on + strand at 2005.508 kb on - strand at 2005.537 kb on + strand at 2005.537 kb on + strand at 2005.537 kb on + strand at 2005.538 kb on - strand at 2005.538 kb on - strand at 2005.538 kb on - strand at 2005.538 kb on - strand at 2005.538 kb on - strand at 2005.538 kb on - strand at 2005.671 kb on + strand, within sdhE at 2005.707 kb on - strand, within sdhE at 2005.908 kb on + strand, within ygfX at 2005.914 kb on - strand, within ygfX at 2005.936 kb on + strand, within ygfX at 2005.958 kb on + strand, within ygfX at 2005.959 kb on - strand, within ygfX at 2005.959 kb on - strand, within ygfX at 2006.001 kb on + strand, within ygfX at 2006.002 kb on - strand, within ygfX at 2006.004 kb on + strand, within ygfX at 2006.004 kb on + strand, within ygfX at 2006.004 kb on + strand, within ygfX at 2006.005 kb on - strand, within ygfX at 2006.005 kb on - strand, within ygfX at 2006.120 kb on + strand, within ygfX at 2006.121 kb on - strand, within ygfX at 2006.150 kb on + strand, within ygfX at 2006.151 kb on - strand, within ygfX at 2006.151 kb on - strand, within ygfX at 2006.230 kb on + strand at 2006.230 kb on + strand at 2006.249 kb on + strand at 2006.249 kb on + strand at 2006.249 kb on + strand at 2006.249 kb on + strand at 2006.249 kb on + strand at 2006.250 kb on - strand at 2006.271 kb on - strand at 2006.280 kb on - strand at 2006.346 kb on - strand, within fldB at 2006.424 kb on - strand, within fldB at 2006.657 kb on + strand, within fldB at 2006.657 kb on + strand, within fldB at 2006.694 kb on + strand, within fldB at 2006.695 kb on - strand, within fldB at 2006.698 kb on + strand, within fldB at 2006.699 kb on - strand, within fldB at 2006.699 kb on - strand, within fldB at 2006.756 kb on + strand at 2006.798 kb on + strand at 2006.798 kb on + strand at 2006.799 kb on - strand at 2006.799 kb on - strand at 2006.799 kb on - strand at 2006.799 kb on - strand at 2006.836 kb on + strand at 2006.837 kb on - strand at 2006.865 kb on - strand at 2006.865 kb on - strand at 2006.871 kb on + strand at 2006.871 kb on + strand at 2006.872 kb on - strand at 2006.872 kb on - strand at 2006.872 kb on - strand at 2006.872 kb on - strand at 2006.893 kb on + strand at 2007.269 kb on + strand, within xerD at 2007.310 kb on - strand, within xerD at 2007.315 kb on + strand, within xerD at 2007.592 kb on + strand, within xerD
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 2,005,339 - +0.9 2,005,339 - -1.4 2,005,364 - +0.1 2,005,367 - +0.6 2,005,370 - +0.3 2,005,370 - -0.2 2,005,370 - +0.4 2,005,370 - +0.6 2,005,374 - +0.0 2,005,374 - +0.1 2,005,374 - -1.3 2,005,380 - -0.1 2,005,388 + +2.3 2,005,388 + +0.8 2,005,388 + -1.0 2,005,389 - +0.3 2,005,502 - +0.8 2,005,507 + -0.5 2,005,507 + -0.1 2,005,507 + +0.3 2,005,508 - -0.9 2,005,537 + -2.5 2,005,537 + +0.4 2,005,537 + +0.6 2,005,538 - -1.3 2,005,538 - +0.2 2,005,538 - -0.6 2,005,538 - -0.1 2,005,538 - +0.6 2,005,538 - +0.9 2,005,671 + sdhE MCAODC_10075 0.35 -0.6 2,005,707 - sdhE MCAODC_10075 0.48 +0.8 2,005,908 + ygfX MCAODC_10080 0.20 -0.2 2,005,914 - ygfX MCAODC_10080 0.22 -0.2 2,005,936 + ygfX MCAODC_10080 0.27 +0.5 2,005,958 + ygfX MCAODC_10080 0.33 +1.2 2,005,959 - ygfX MCAODC_10080 0.33 +0.1 2,005,959 - ygfX MCAODC_10080 0.33 -0.0 2,006,001 + ygfX MCAODC_10080 0.43 -2.0 2,006,002 - ygfX MCAODC_10080 0.43 +1.5 2,006,004 + ygfX MCAODC_10080 0.44 +2.1 2,006,004 + ygfX MCAODC_10080 0.44 -0.2 2,006,004 + ygfX MCAODC_10080 0.44 -2.2 2,006,005 - ygfX MCAODC_10080 0.44 -0.6 2,006,005 - ygfX MCAODC_10080 0.44 +1.0 2,006,120 + ygfX MCAODC_10080 0.72 +0.1 2,006,121 - ygfX MCAODC_10080 0.73 -1.1 2,006,150 + ygfX MCAODC_10080 0.80 -0.3 2,006,151 - ygfX MCAODC_10080 0.80 +0.0 2,006,151 - ygfX MCAODC_10080 0.80 +0.6 2,006,230 + +0.1 2,006,230 + -0.7 2,006,249 + +0.0 2,006,249 + -0.2 2,006,249 + +0.4 2,006,249 + +1.0 2,006,249 + -0.6 2,006,250 - +1.3 2,006,271 - -4.6 2,006,280 - -0.4 2,006,346 - fldB MCAODC_10085 0.14 -1.8 2,006,424 - fldB MCAODC_10085 0.29 +3.1 2,006,657 + fldB MCAODC_10085 0.74 -0.7 2,006,657 + fldB MCAODC_10085 0.74 -1.6 2,006,694 + fldB MCAODC_10085 0.81 -0.1 2,006,695 - fldB MCAODC_10085 0.81 +0.9 2,006,698 + fldB MCAODC_10085 0.82 -1.0 2,006,699 - fldB MCAODC_10085 0.82 -1.3 2,006,699 - fldB MCAODC_10085 0.82 +0.4 2,006,756 + +2.6 2,006,798 + -0.4 2,006,798 + -1.0 2,006,799 - -0.5 2,006,799 - +2.4 2,006,799 - -0.1 2,006,799 - -1.0 2,006,836 + -0.7 2,006,837 - +0.5 2,006,865 - +0.1 2,006,865 - +1.2 2,006,871 + +0.1 2,006,871 + -0.7 2,006,872 - -0.9 2,006,872 - -2.0 2,006,872 - -1.9 2,006,872 - -4.3 2,006,893 + -0.6 2,007,269 + xerD MCAODC_10090 0.41 -1.6 2,007,310 - xerD MCAODC_10090 0.45 -1.8 2,007,315 + xerD MCAODC_10090 0.46 -0.9 2,007,592 + xerD MCAODC_10090 0.77 -0.7
Or see this region's nucleotide sequence