Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fbaA and mscS are separated by 357 nucleotides mscS and argO are separated by 138 nucleotides argO and yggE are separated by 92 nucleotides
MCAODC_09915: fbaA - class II fructose-bisphosphate aldolase, at 1,976,077 to 1,977,156
fbaA
MCAODC_09925: mscS - small-conductance mechanosensitive channel MscS, at 1,977,514 to 1,978,374
mscS
MCAODC_09930: argO - arginine exporter ArgO, at 1,978,513 to 1,979,148
argO
MCAODC_09935: yggE - oxidative stress defense protein, at 1,979,241 to 1,979,981
yggE
Position (kb)
1977
1978
1979 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1977.154 kb on + strand at 1977.155 kb on - strand at 1977.173 kb on + strand at 1977.173 kb on + strand at 1977.174 kb on - strand at 1977.174 kb on - strand at 1977.239 kb on - strand at 1977.338 kb on + strand at 1977.339 kb on - strand at 1977.339 kb on - strand at 1977.411 kb on + strand at 1977.412 kb on - strand at 1977.424 kb on + strand at 1977.431 kb on + strand at 1977.432 kb on - strand at 1977.432 kb on - strand at 1977.432 kb on - strand at 1977.432 kb on - strand at 1977.476 kb on + strand at 1977.508 kb on + strand at 1977.508 kb on + strand at 1977.508 kb on + strand at 1977.567 kb on - strand at 1977.600 kb on - strand, within mscS at 1977.600 kb on - strand, within mscS at 1977.728 kb on + strand, within mscS at 1977.744 kb on + strand, within mscS at 1977.827 kb on + strand, within mscS at 1977.827 kb on + strand, within mscS at 1977.828 kb on - strand, within mscS at 1977.828 kb on - strand, within mscS at 1977.862 kb on - strand, within mscS at 1977.882 kb on - strand, within mscS at 1977.916 kb on + strand, within mscS at 1977.917 kb on - strand, within mscS at 1977.917 kb on - strand, within mscS at 1977.942 kb on + strand, within mscS at 1978.017 kb on + strand, within mscS at 1978.035 kb on + strand, within mscS at 1978.041 kb on + strand, within mscS at 1978.042 kb on - strand, within mscS at 1978.077 kb on + strand, within mscS at 1978.077 kb on + strand, within mscS at 1978.080 kb on + strand, within mscS at 1978.093 kb on + strand, within mscS at 1978.134 kb on + strand, within mscS at 1978.137 kb on + strand, within mscS at 1978.261 kb on + strand, within mscS at 1978.281 kb on + strand, within mscS at 1978.281 kb on + strand, within mscS at 1978.284 kb on + strand, within mscS at 1978.284 kb on + strand, within mscS at 1978.285 kb on - strand, within mscS at 1978.392 kb on + strand at 1978.396 kb on - strand at 1978.396 kb on - strand at 1978.437 kb on + strand at 1978.438 kb on - strand at 1978.459 kb on + strand at 1978.460 kb on - strand at 1978.473 kb on + strand at 1978.474 kb on - strand at 1978.475 kb on + strand at 1978.554 kb on + strand at 1978.624 kb on - strand, within argO at 1978.624 kb on - strand, within argO at 1978.634 kb on + strand, within argO at 1978.634 kb on + strand, within argO at 1978.634 kb on + strand, within argO at 1978.686 kb on + strand, within argO at 1978.742 kb on + strand, within argO at 1978.743 kb on - strand, within argO at 1978.743 kb on - strand, within argO at 1978.743 kb on - strand, within argO at 1978.794 kb on + strand, within argO at 1978.795 kb on - strand, within argO at 1978.795 kb on - strand, within argO at 1978.797 kb on + strand, within argO at 1978.797 kb on + strand, within argO at 1978.798 kb on - strand, within argO at 1978.805 kb on + strand, within argO at 1978.806 kb on - strand, within argO at 1978.806 kb on - strand, within argO at 1978.809 kb on + strand, within argO at 1978.810 kb on - strand, within argO at 1978.810 kb on - strand, within argO at 1978.810 kb on - strand, within argO at 1978.810 kb on - strand, within argO at 1978.810 kb on - strand, within argO at 1978.810 kb on - strand, within argO at 1978.845 kb on + strand, within argO at 1978.889 kb on - strand, within argO at 1978.892 kb on + strand, within argO at 1978.893 kb on - strand, within argO at 1978.893 kb on - strand, within argO at 1978.896 kb on + strand, within argO at 1978.896 kb on + strand, within argO at 1978.897 kb on - strand, within argO at 1978.907 kb on + strand, within argO at 1978.908 kb on - strand, within argO at 1979.005 kb on - strand, within argO at 1979.058 kb on + strand, within argO at 1979.058 kb on + strand, within argO at 1979.059 kb on - strand, within argO at 1979.059 kb on - strand, within argO at 1979.059 kb on - strand, within argO at 1979.091 kb on + strand at 1979.091 kb on + strand at 1979.119 kb on - strand at 1979.154 kb on - strand at 1979.182 kb on + strand at 1979.223 kb on + strand at 1979.224 kb on - strand at 1979.327 kb on + strand, within yggE at 1979.327 kb on + strand, within yggE at 1979.328 kb on - strand, within yggE at 1979.328 kb on - strand, within yggE at 1979.328 kb on - strand, within yggE
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 1,977,154 + -0.2 1,977,155 - -0.7 1,977,173 + +0.3 1,977,173 + -1.8 1,977,174 - +4.0 1,977,174 - -0.3 1,977,239 - +0.3 1,977,338 + -0.1 1,977,339 - -0.5 1,977,339 - +0.4 1,977,411 + +1.0 1,977,412 - -0.5 1,977,424 + -0.8 1,977,431 + -0.4 1,977,432 - -2.9 1,977,432 - -3.1 1,977,432 - +0.1 1,977,432 - +2.1 1,977,476 + -0.1 1,977,508 + -2.1 1,977,508 + +0.2 1,977,508 + -0.2 1,977,567 - -0.4 1,977,600 - mscS MCAODC_09925 0.10 +0.1 1,977,600 - mscS MCAODC_09925 0.10 +0.1 1,977,728 + mscS MCAODC_09925 0.25 -0.0 1,977,744 + mscS MCAODC_09925 0.27 +0.2 1,977,827 + mscS MCAODC_09925 0.36 -0.6 1,977,827 + mscS MCAODC_09925 0.36 -0.0 1,977,828 - mscS MCAODC_09925 0.36 -0.2 1,977,828 - mscS MCAODC_09925 0.36 +0.9 1,977,862 - mscS MCAODC_09925 0.40 -0.4 1,977,882 - mscS MCAODC_09925 0.43 +1.6 1,977,916 + mscS MCAODC_09925 0.47 -1.0 1,977,917 - mscS MCAODC_09925 0.47 -0.9 1,977,917 - mscS MCAODC_09925 0.47 +0.8 1,977,942 + mscS MCAODC_09925 0.50 -1.0 1,978,017 + mscS MCAODC_09925 0.58 -0.4 1,978,035 + mscS MCAODC_09925 0.61 -0.6 1,978,041 + mscS MCAODC_09925 0.61 +0.6 1,978,042 - mscS MCAODC_09925 0.61 -0.5 1,978,077 + mscS MCAODC_09925 0.65 +1.6 1,978,077 + mscS MCAODC_09925 0.65 -1.2 1,978,080 + mscS MCAODC_09925 0.66 +0.8 1,978,093 + mscS MCAODC_09925 0.67 -0.2 1,978,134 + mscS MCAODC_09925 0.72 -1.1 1,978,137 + mscS MCAODC_09925 0.72 +0.2 1,978,261 + mscS MCAODC_09925 0.87 -2.1 1,978,281 + mscS MCAODC_09925 0.89 +0.7 1,978,281 + mscS MCAODC_09925 0.89 +1.0 1,978,284 + mscS MCAODC_09925 0.89 -0.2 1,978,284 + mscS MCAODC_09925 0.89 +0.7 1,978,285 - mscS MCAODC_09925 0.90 -0.3 1,978,392 + -0.3 1,978,396 - +0.8 1,978,396 - -1.5 1,978,437 + +0.1 1,978,438 - +0.2 1,978,459 + +0.2 1,978,460 - +1.1 1,978,473 + +0.8 1,978,474 - +0.2 1,978,475 + +0.4 1,978,554 + -1.0 1,978,624 - argO MCAODC_09930 0.17 +0.5 1,978,624 - argO MCAODC_09930 0.17 -0.9 1,978,634 + argO MCAODC_09930 0.19 -0.5 1,978,634 + argO MCAODC_09930 0.19 +0.9 1,978,634 + argO MCAODC_09930 0.19 +0.5 1,978,686 + argO MCAODC_09930 0.27 -0.5 1,978,742 + argO MCAODC_09930 0.36 +0.0 1,978,743 - argO MCAODC_09930 0.36 +0.8 1,978,743 - argO MCAODC_09930 0.36 +2.1 1,978,743 - argO MCAODC_09930 0.36 +0.4 1,978,794 + argO MCAODC_09930 0.44 -0.1 1,978,795 - argO MCAODC_09930 0.44 -0.9 1,978,795 - argO MCAODC_09930 0.44 -0.4 1,978,797 + argO MCAODC_09930 0.45 -1.2 1,978,797 + argO MCAODC_09930 0.45 +1.0 1,978,798 - argO MCAODC_09930 0.45 +1.6 1,978,805 + argO MCAODC_09930 0.46 +0.7 1,978,806 - argO MCAODC_09930 0.46 -1.1 1,978,806 - argO MCAODC_09930 0.46 +1.4 1,978,809 + argO MCAODC_09930 0.47 +0.2 1,978,810 - argO MCAODC_09930 0.47 +0.2 1,978,810 - argO MCAODC_09930 0.47 +0.4 1,978,810 - argO MCAODC_09930 0.47 -0.5 1,978,810 - argO MCAODC_09930 0.47 +1.0 1,978,810 - argO MCAODC_09930 0.47 +1.3 1,978,810 - argO MCAODC_09930 0.47 -0.4 1,978,845 + argO MCAODC_09930 0.52 -1.1 1,978,889 - argO MCAODC_09930 0.59 -0.3 1,978,892 + argO MCAODC_09930 0.60 -0.7 1,978,893 - argO MCAODC_09930 0.60 +0.8 1,978,893 - argO MCAODC_09930 0.60 -0.2 1,978,896 + argO MCAODC_09930 0.60 -0.0 1,978,896 + argO MCAODC_09930 0.60 +0.1 1,978,897 - argO MCAODC_09930 0.60 +1.5 1,978,907 + argO MCAODC_09930 0.62 -2.0 1,978,908 - argO MCAODC_09930 0.62 -0.3 1,979,005 - argO MCAODC_09930 0.77 +1.1 1,979,058 + argO MCAODC_09930 0.86 -0.8 1,979,058 + argO MCAODC_09930 0.86 +0.2 1,979,059 - argO MCAODC_09930 0.86 +1.1 1,979,059 - argO MCAODC_09930 0.86 -3.0 1,979,059 - argO MCAODC_09930 0.86 -2.0 1,979,091 + +0.6 1,979,091 + -0.2 1,979,119 - -1.0 1,979,154 - -1.2 1,979,182 + -0.4 1,979,223 + -0.1 1,979,224 - +0.7 1,979,327 + yggE MCAODC_09935 0.12 -0.9 1,979,327 + yggE MCAODC_09935 0.12 -0.6 1,979,328 - yggE MCAODC_09935 0.12 +1.0 1,979,328 - yggE MCAODC_09935 0.12 +0.2 1,979,328 - yggE MCAODC_09935 0.12 +0.3
Or see this region's nucleotide sequence