Strain Fitness in Escherichia coli ECRC101 around MCAODC_09555

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthybD and hybE overlap by 8 nucleotideshybE and hypA overlap by 8 nucleotideshypA and hybG are separated by 12 nucleotideshybG and yghU are separated by 122 nucleotides MCAODC_09540: hybD - HyaD/HybD family hydrogenase maturation endopeptidase, at 1,903,128 to 1,903,622 hybD MCAODC_09545: hybE - hydrogenase-2 assembly chaperone, at 1,903,615 to 1,904,103 hybE MCAODC_09550: hypA - hydrogenase maturation nickel metallochaperone HypA, at 1,904,096 to 1,904,437 hypA MCAODC_09555: hybG - hydrogenase maturation factor HybG, at 1,904,450 to 1,904,698 hybG MCAODC_09565: yghU - glutathione-dependent disulfide-bond oxidoreductase, at 1,904,821 to 1,905,687 yghU Position (kb) 1904 1905Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 1903.483 kb on + strand, within hybDat 1903.628 kb on + strandat 1903.629 kb on - strandat 1903.662 kb on - strandat 1903.662 kb on - strandat 1903.735 kb on + strand, within hybEat 1903.735 kb on + strand, within hybEat 1903.736 kb on - strand, within hybEat 1903.809 kb on + strand, within hybEat 1903.809 kb on + strand, within hybEat 1903.880 kb on - strand, within hybEat 1903.927 kb on + strand, within hybEat 1903.965 kb on + strand, within hybEat 1903.965 kb on + strand, within hybEat 1903.966 kb on - strand, within hybEat 1904.165 kb on - strand, within hypAat 1904.233 kb on + strand, within hypAat 1904.280 kb on + strand, within hypAat 1904.280 kb on + strand, within hypAat 1904.280 kb on + strand, within hypAat 1904.281 kb on - strand, within hypAat 1904.281 kb on - strand, within hypAat 1904.284 kb on + strand, within hypAat 1904.284 kb on + strand, within hypAat 1904.284 kb on + strand, within hypAat 1904.285 kb on - strand, within hypAat 1904.285 kb on - strand, within hypAat 1904.291 kb on + strand, within hypAat 1904.422 kb on + strandat 1904.422 kb on + strandat 1904.422 kb on + strandat 1904.423 kb on - strandat 1904.445 kb on + strandat 1904.455 kb on + strandat 1904.455 kb on + strandat 1904.455 kb on + strandat 1904.456 kb on - strandat 1904.494 kb on + strand, within hybGat 1904.495 kb on - strand, within hybGat 1904.520 kb on + strand, within hybGat 1904.520 kb on + strand, within hybGat 1904.520 kb on + strand, within hybGat 1904.520 kb on + strand, within hybGat 1904.520 kb on + strand, within hybGat 1904.521 kb on - strand, within hybGat 1904.521 kb on - strand, within hybGat 1904.521 kb on - strand, within hybGat 1904.521 kb on - strand, within hybGat 1904.567 kb on - strand, within hybGat 1904.679 kb on - strandat 1904.713 kb on - strandat 1904.836 kb on - strandat 1904.836 kb on - strandat 1904.836 kb on - strandat 1904.979 kb on + strand, within yghUat 1904.980 kb on - strand, within yghUat 1904.980 kb on - strand, within yghUat 1904.980 kb on - strand, within yghUat 1904.982 kb on - strand, within yghUat 1904.982 kb on - strand, within yghUat 1905.014 kb on + strand, within yghUat 1905.014 kb on + strand, within yghUat 1905.015 kb on - strand, within yghUat 1905.015 kb on - strand, within yghUat 1905.015 kb on - strand, within yghUat 1905.074 kb on + strand, within yghUat 1905.074 kb on + strand, within yghUat 1905.074 kb on + strand, within yghUat 1905.074 kb on + strand, within yghUat 1905.074 kb on + strand, within yghUat 1905.075 kb on - strand, within yghUat 1905.075 kb on - strand, within yghUat 1905.096 kb on + strand, within yghUat 1905.096 kb on + strand, within yghUat 1905.096 kb on + strand, within yghUat 1905.096 kb on + strand, within yghUat 1905.097 kb on - strand, within yghUat 1905.097 kb on - strand, within yghUat 1905.150 kb on + strand, within yghUat 1905.194 kb on + strand, within yghUat 1905.297 kb on - strand, within yghUat 1905.318 kb on - strand, within yghUat 1905.360 kb on + strand, within yghUat 1905.366 kb on + strand, within yghUat 1905.448 kb on + strand, within yghUat 1905.464 kb on + strand, within yghUat 1905.464 kb on + strand, within yghUat 1905.465 kb on - strand, within yghUat 1905.507 kb on + strand, within yghUat 1905.514 kb on + strand, within yghUat 1905.514 kb on + strand, within yghUat 1905.554 kb on - strand, within yghUat 1905.554 kb on - strand, within yghUat 1905.616 kb on - strandat 1905.671 kb on + strandat 1905.671 kb on + strandat 1905.672 kb on - strandat 1905.672 kb on - strandat 1905.672 kb on - strandat 1905.672 kb on - strandat 1905.675 kb on + strandat 1905.675 kb on + strandat 1905.675 kb on + strandat 1905.675 kb on + strandat 1905.675 kb on + strandat 1905.675 kb on + strandat 1905.676 kb on - strandat 1905.687 kb on + strandat 1905.687 kb on + strandat 1905.687 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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1,903,483 + hybD MCAODC_09540 0.72 +0.7
1,903,628 + -1.1
1,903,629 - -0.7
1,903,662 - -0.4
1,903,662 - -2.0
1,903,735 + hybE MCAODC_09545 0.25 -3.4
1,903,735 + hybE MCAODC_09545 0.25 +0.5
1,903,736 - hybE MCAODC_09545 0.25 +0.2
1,903,809 + hybE MCAODC_09545 0.40 -0.0
1,903,809 + hybE MCAODC_09545 0.40 -0.7
1,903,880 - hybE MCAODC_09545 0.54 -0.6
1,903,927 + hybE MCAODC_09545 0.64 -0.1
1,903,965 + hybE MCAODC_09545 0.72 +0.7
1,903,965 + hybE MCAODC_09545 0.72 -1.8
1,903,966 - hybE MCAODC_09545 0.72 -2.0
1,904,165 - hypA MCAODC_09550 0.20 -0.4
1,904,233 + hypA MCAODC_09550 0.40 -0.7
1,904,280 + hypA MCAODC_09550 0.54 -2.2
1,904,280 + hypA MCAODC_09550 0.54 +1.3
1,904,280 + hypA MCAODC_09550 0.54 -4.4
1,904,281 - hypA MCAODC_09550 0.54 +0.4
1,904,281 - hypA MCAODC_09550 0.54 -0.3
1,904,284 + hypA MCAODC_09550 0.55 -0.3
1,904,284 + hypA MCAODC_09550 0.55 -0.4
1,904,284 + hypA MCAODC_09550 0.55 -0.5
1,904,285 - hypA MCAODC_09550 0.55 -3.1
1,904,285 - hypA MCAODC_09550 0.55 -0.3
1,904,291 + hypA MCAODC_09550 0.57 -1.4
1,904,422 + +0.2
1,904,422 + +0.3
1,904,422 + +0.8
1,904,423 - +0.2
1,904,445 + +0.3
1,904,455 + -1.5
1,904,455 + +0.2
1,904,455 + -0.6
1,904,456 - -1.6
1,904,494 + hybG MCAODC_09555 0.18 +1.6
1,904,495 - hybG MCAODC_09555 0.18 -0.2
1,904,520 + hybG MCAODC_09555 0.28 +0.3
1,904,520 + hybG MCAODC_09555 0.28 -0.7
1,904,520 + hybG MCAODC_09555 0.28 +0.1
1,904,520 + hybG MCAODC_09555 0.28 -0.7
1,904,520 + hybG MCAODC_09555 0.28 -0.8
1,904,521 - hybG MCAODC_09555 0.29 +0.3
1,904,521 - hybG MCAODC_09555 0.29 +0.2
1,904,521 - hybG MCAODC_09555 0.29 +0.9
1,904,521 - hybG MCAODC_09555 0.29 +0.5
1,904,567 - hybG MCAODC_09555 0.47 +2.3
1,904,679 - +0.5
1,904,713 - -0.3
1,904,836 - +1.2
1,904,836 - +1.0
1,904,836 - +0.0
1,904,979 + yghU MCAODC_09565 0.18 +1.2
1,904,980 - yghU MCAODC_09565 0.18 +0.3
1,904,980 - yghU MCAODC_09565 0.18 +0.4
1,904,980 - yghU MCAODC_09565 0.18 +0.5
1,904,982 - yghU MCAODC_09565 0.19 -0.6
1,904,982 - yghU MCAODC_09565 0.19 -0.7
1,905,014 + yghU MCAODC_09565 0.22 +0.1
1,905,014 + yghU MCAODC_09565 0.22 +0.8
1,905,015 - yghU MCAODC_09565 0.22 -0.0
1,905,015 - yghU MCAODC_09565 0.22 +0.4
1,905,015 - yghU MCAODC_09565 0.22 +0.5
1,905,074 + yghU MCAODC_09565 0.29 -2.3
1,905,074 + yghU MCAODC_09565 0.29 +0.9
1,905,074 + yghU MCAODC_09565 0.29 -1.2
1,905,074 + yghU MCAODC_09565 0.29 +1.3
1,905,074 + yghU MCAODC_09565 0.29 -0.7
1,905,075 - yghU MCAODC_09565 0.29 -0.1
1,905,075 - yghU MCAODC_09565 0.29 +0.0
1,905,096 + yghU MCAODC_09565 0.32 -0.3
1,905,096 + yghU MCAODC_09565 0.32 -0.0
1,905,096 + yghU MCAODC_09565 0.32 -0.0
1,905,096 + yghU MCAODC_09565 0.32 +0.7
1,905,097 - yghU MCAODC_09565 0.32 -0.3
1,905,097 - yghU MCAODC_09565 0.32 +0.2
1,905,150 + yghU MCAODC_09565 0.38 +1.4
1,905,194 + yghU MCAODC_09565 0.43 +0.8
1,905,297 - yghU MCAODC_09565 0.55 -0.8
1,905,318 - yghU MCAODC_09565 0.57 -0.1
1,905,360 + yghU MCAODC_09565 0.62 +1.5
1,905,366 + yghU MCAODC_09565 0.63 -1.3
1,905,448 + yghU MCAODC_09565 0.72 +0.2
1,905,464 + yghU MCAODC_09565 0.74 +1.0
1,905,464 + yghU MCAODC_09565 0.74 -1.0
1,905,465 - yghU MCAODC_09565 0.74 +0.5
1,905,507 + yghU MCAODC_09565 0.79 -1.2
1,905,514 + yghU MCAODC_09565 0.80 -0.2
1,905,514 + yghU MCAODC_09565 0.80 +0.2
1,905,554 - yghU MCAODC_09565 0.85 +3.1
1,905,554 - yghU MCAODC_09565 0.85 +0.7
1,905,616 - -1.0
1,905,671 + -0.1
1,905,671 + +0.3
1,905,672 - +0.2
1,905,672 - +0.2
1,905,672 - +0.5
1,905,672 - -0.4
1,905,675 + -0.5
1,905,675 + -0.0
1,905,675 + -0.4
1,905,675 + -0.0
1,905,675 + +0.1
1,905,675 + +0.3
1,905,676 - -2.0
1,905,687 + -1.1
1,905,687 + -1.2
1,905,687 + -0.3

Or see this region's nucleotide sequence