Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt adiA and adiY are separated by 324 nucleotides adiY and adiC are separated by 136 nucleotides
MCAODC_03060: adiA - arginine decarboxylase, at 619,095 to 621,362
adiA
MCAODC_03065: adiY - DNA-binding transcriptional activator AdiY, at 621,687 to 622,448
adiY
MCAODC_03070: adiC - arginine/agmatine antiporter, at 622,585 to 623,922
adiC
Position (kb) 621
622
623 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 620.829 kb on - strand, within adiA at 620.843 kb on + strand, within adiA at 620.852 kb on - strand, within adiA at 620.908 kb on - strand, within adiA at 620.959 kb on - strand, within adiA at 620.970 kb on + strand, within adiA at 620.971 kb on - strand, within adiA at 621.057 kb on + strand, within adiA at 621.099 kb on + strand, within adiA at 621.248 kb on - strand at 621.252 kb on + strand at 621.252 kb on + strand at 621.252 kb on + strand at 621.253 kb on - strand at 621.257 kb on - strand at 621.324 kb on - strand at 621.324 kb on - strand at 621.333 kb on - strand at 621.344 kb on + strand at 621.344 kb on + strand at 621.345 kb on - strand at 621.391 kb on + strand at 621.391 kb on + strand at 621.395 kb on + strand at 621.402 kb on - strand at 621.616 kb on - strand at 621.680 kb on + strand at 621.681 kb on - strand at 621.686 kb on + strand at 621.686 kb on + strand at 621.687 kb on - strand at 621.713 kb on + strand at 621.714 kb on - strand at 621.714 kb on - strand at 621.767 kb on + strand, within adiY at 621.770 kb on + strand, within adiY at 621.771 kb on - strand, within adiY at 621.799 kb on + strand, within adiY at 621.857 kb on + strand, within adiY at 621.900 kb on - strand, within adiY at 621.932 kb on + strand, within adiY at 622.100 kb on - strand, within adiY at 622.110 kb on - strand, within adiY at 622.118 kb on + strand, within adiY at 622.181 kb on + strand, within adiY at 622.184 kb on + strand, within adiY at 622.185 kb on - strand, within adiY at 622.202 kb on - strand, within adiY at 622.255 kb on - strand, within adiY at 622.255 kb on - strand, within adiY at 622.257 kb on - strand, within adiY at 622.304 kb on - strand, within adiY at 622.322 kb on + strand, within adiY at 622.382 kb on + strand at 622.413 kb on + strand at 622.413 kb on + strand at 622.413 kb on + strand at 622.419 kb on - strand at 622.419 kb on - strand at 622.459 kb on + strand at 622.470 kb on + strand at 622.470 kb on + strand at 622.470 kb on + strand at 622.470 kb on + strand at 622.470 kb on + strand at 622.471 kb on - strand at 622.471 kb on - strand at 622.472 kb on + strand at 622.476 kb on - strand at 622.476 kb on - strand at 622.508 kb on + strand at 622.509 kb on - strand at 622.532 kb on + strand at 622.538 kb on - strand at 622.619 kb on + strand at 622.654 kb on - strand at 622.714 kb on + strand at 622.714 kb on + strand at 622.714 kb on + strand at 622.715 kb on - strand at 622.715 kb on - strand at 622.761 kb on - strand, within adiC at 622.761 kb on - strand, within adiC at 622.763 kb on - strand, within adiC at 622.804 kb on + strand, within adiC at 622.805 kb on - strand, within adiC at 622.805 kb on - strand, within adiC at 622.805 kb on - strand, within adiC at 622.810 kb on + strand, within adiC at 622.916 kb on - strand, within adiC at 622.919 kb on + strand, within adiC at 622.919 kb on + strand, within adiC at 622.919 kb on + strand, within adiC at 622.920 kb on - strand, within adiC at 622.920 kb on - strand, within adiC at 622.920 kb on - strand, within adiC at 622.924 kb on + strand, within adiC at 622.925 kb on - strand, within adiC at 622.925 kb on - strand, within adiC at 622.925 kb on - strand, within adiC at 623.084 kb on + strand, within adiC at 623.085 kb on - strand, within adiC at 623.116 kb on + strand, within adiC at 623.116 kb on + strand, within adiC at 623.116 kb on + strand, within adiC at 623.116 kb on + strand, within adiC at 623.116 kb on + strand, within adiC at 623.116 kb on + strand, within adiC at 623.117 kb on - strand, within adiC at 623.117 kb on - strand, within adiC at 623.170 kb on - strand, within adiC at 623.170 kb on - strand, within adiC at 623.170 kb on - strand, within adiC at 623.175 kb on + strand, within adiC at 623.175 kb on + strand, within adiC at 623.176 kb on - strand, within adiC at 623.182 kb on - strand, within adiC at 623.259 kb on + strand, within adiC at 623.261 kb on + strand, within adiC at 623.300 kb on - strand, within adiC at 623.300 kb on - strand, within adiC at 623.300 kb on - strand, within adiC at 623.300 kb on - strand, within adiC at 623.337 kb on + strand, within adiC at 623.444 kb on + strand, within adiC at 623.444 kb on + strand, within adiC at 623.445 kb on - strand, within adiC at 623.445 kb on - strand, within adiC at 623.445 kb on - strand, within adiC
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 620,829 - adiA MCAODC_03060 0.76 +0.5 620,843 + adiA MCAODC_03060 0.77 +0.8 620,852 - adiA MCAODC_03060 0.77 +0.4 620,908 - adiA MCAODC_03060 0.80 -2.5 620,959 - adiA MCAODC_03060 0.82 -0.2 620,970 + adiA MCAODC_03060 0.83 +0.8 620,971 - adiA MCAODC_03060 0.83 -0.2 621,057 + adiA MCAODC_03060 0.87 -0.4 621,099 + adiA MCAODC_03060 0.88 +0.1 621,248 - +1.0 621,252 + +0.3 621,252 + +0.5 621,252 + -0.9 621,253 - -0.6 621,257 - +1.4 621,324 - +0.7 621,324 - -1.4 621,333 - -0.4 621,344 + +0.0 621,344 + -1.5 621,345 - +0.8 621,391 + -1.2 621,391 + -0.5 621,395 + +0.4 621,402 - +0.1 621,616 - -0.1 621,680 + +0.1 621,681 - -0.7 621,686 + +0.0 621,686 + +0.5 621,687 - -1.1 621,713 + -1.3 621,714 - +0.4 621,714 - +1.2 621,767 + adiY MCAODC_03065 0.10 -0.3 621,770 + adiY MCAODC_03065 0.11 +0.6 621,771 - adiY MCAODC_03065 0.11 -0.2 621,799 + adiY MCAODC_03065 0.15 -0.9 621,857 + adiY MCAODC_03065 0.22 +0.2 621,900 - adiY MCAODC_03065 0.28 -1.3 621,932 + adiY MCAODC_03065 0.32 -0.8 622,100 - adiY MCAODC_03065 0.54 -3.4 622,110 - adiY MCAODC_03065 0.56 +0.3 622,118 + adiY MCAODC_03065 0.57 -0.5 622,181 + adiY MCAODC_03065 0.65 +0.3 622,184 + adiY MCAODC_03065 0.65 +1.2 622,185 - adiY MCAODC_03065 0.65 -1.0 622,202 - adiY MCAODC_03065 0.68 -1.5 622,255 - adiY MCAODC_03065 0.75 +0.3 622,255 - adiY MCAODC_03065 0.75 -0.3 622,257 - adiY MCAODC_03065 0.75 -0.5 622,304 - adiY MCAODC_03065 0.81 +0.7 622,322 + adiY MCAODC_03065 0.83 +0.4 622,382 + +0.4 622,413 + +1.1 622,413 + -0.3 622,413 + +0.5 622,419 - +1.5 622,419 - -0.3 622,459 + -0.2 622,470 + -1.1 622,470 + -0.2 622,470 + +1.2 622,470 + +1.0 622,470 + +0.7 622,471 - -0.4 622,471 - +1.3 622,472 + +0.9 622,476 - -1.7 622,476 - +0.5 622,508 + -0.2 622,509 - -1.3 622,532 + -2.2 622,538 - -2.6 622,619 + +0.4 622,654 - +0.6 622,714 + +0.5 622,714 + +0.2 622,714 + +0.2 622,715 - +0.8 622,715 - -2.8 622,761 - adiC MCAODC_03070 0.13 +0.1 622,761 - adiC MCAODC_03070 0.13 -1.2 622,763 - adiC MCAODC_03070 0.13 -0.5 622,804 + adiC MCAODC_03070 0.16 +0.1 622,805 - adiC MCAODC_03070 0.16 -0.2 622,805 - adiC MCAODC_03070 0.16 +0.0 622,805 - adiC MCAODC_03070 0.16 +0.3 622,810 + adiC MCAODC_03070 0.17 +0.9 622,916 - adiC MCAODC_03070 0.25 +0.6 622,919 + adiC MCAODC_03070 0.25 +0.0 622,919 + adiC MCAODC_03070 0.25 +0.5 622,919 + adiC MCAODC_03070 0.25 +1.1 622,920 - adiC MCAODC_03070 0.25 -0.4 622,920 - adiC MCAODC_03070 0.25 -0.5 622,920 - adiC MCAODC_03070 0.25 -2.1 622,924 + adiC MCAODC_03070 0.25 -1.4 622,925 - adiC MCAODC_03070 0.25 -0.2 622,925 - adiC MCAODC_03070 0.25 -0.4 622,925 - adiC MCAODC_03070 0.25 -0.2 623,084 + adiC MCAODC_03070 0.37 -1.3 623,085 - adiC MCAODC_03070 0.37 +0.1 623,116 + adiC MCAODC_03070 0.40 +0.3 623,116 + adiC MCAODC_03070 0.40 +0.6 623,116 + adiC MCAODC_03070 0.40 -1.9 623,116 + adiC MCAODC_03070 0.40 -0.2 623,116 + adiC MCAODC_03070 0.40 +0.1 623,116 + adiC MCAODC_03070 0.40 +0.4 623,117 - adiC MCAODC_03070 0.40 -0.2 623,117 - adiC MCAODC_03070 0.40 +0.1 623,170 - adiC MCAODC_03070 0.44 +0.3 623,170 - adiC MCAODC_03070 0.44 -0.4 623,170 - adiC MCAODC_03070 0.44 +1.0 623,175 + adiC MCAODC_03070 0.44 -0.2 623,175 + adiC MCAODC_03070 0.44 -1.8 623,176 - adiC MCAODC_03070 0.44 +2.1 623,182 - adiC MCAODC_03070 0.45 -1.1 623,259 + adiC MCAODC_03070 0.50 -0.5 623,261 + adiC MCAODC_03070 0.51 -0.3 623,300 - adiC MCAODC_03070 0.53 -0.3 623,300 - adiC MCAODC_03070 0.53 -1.1 623,300 - adiC MCAODC_03070 0.53 -0.3 623,300 - adiC MCAODC_03070 0.53 -2.7 623,337 + adiC MCAODC_03070 0.56 -0.0 623,444 + adiC MCAODC_03070 0.64 +0.4 623,444 + adiC MCAODC_03070 0.64 +0.2 623,445 - adiC MCAODC_03070 0.64 -0.4 623,445 - adiC MCAODC_03070 0.64 -0.1 623,445 - adiC MCAODC_03070 0.64 +0.3
Or see this region's nucleotide sequence