Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ytfI and cysQ are separated by 211 nucleotides cysQ and cpdB are separated by 189 nucleotides
MCAODC_02545: ytfI - Uncharacterized protein YtfI, at 519,957 to 520,895
ytfI
MCAODC_02550: cysQ - 3'(2'),5'-bisphosphate nucleotidase CysQ, at 521,107 to 521,847
cysQ
MCAODC_02560: cpdB - 2',3'-cyclic-nucleotide 2'-phosphodiesterase, at 522,037 to 523,980
cpdB
Position (kb) 521
522 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 520.161 kb on - strand, within ytfI at 520.296 kb on - strand, within ytfI at 520.296 kb on - strand, within ytfI at 520.359 kb on + strand, within ytfI at 520.372 kb on - strand, within ytfI at 520.391 kb on - strand, within ytfI at 520.483 kb on + strand, within ytfI at 520.542 kb on - strand, within ytfI at 520.542 kb on - strand, within ytfI at 520.574 kb on - strand, within ytfI at 520.662 kb on - strand, within ytfI at 520.907 kb on - strand at 520.987 kb on + strand at 521.084 kb on + strand at 521.108 kb on + strand at 521.115 kb on + strand at 521.116 kb on - strand at 521.159 kb on + strand at 521.160 kb on - strand at 521.477 kb on + strand, within cysQ at 521.482 kb on + strand, within cysQ at 521.482 kb on + strand, within cysQ at 521.482 kb on + strand, within cysQ at 521.482 kb on + strand, within cysQ at 521.482 kb on + strand, within cysQ at 521.482 kb on + strand, within cysQ at 521.483 kb on - strand, within cysQ at 521.483 kb on - strand, within cysQ at 521.483 kb on - strand, within cysQ at 521.483 kb on - strand, within cysQ at 521.483 kb on - strand, within cysQ at 521.483 kb on - strand, within cysQ at 521.501 kb on + strand, within cysQ at 521.513 kb on - strand, within cysQ at 521.513 kb on - strand, within cysQ at 521.571 kb on + strand, within cysQ at 521.602 kb on + strand, within cysQ at 521.603 kb on - strand, within cysQ at 521.603 kb on - strand, within cysQ at 521.603 kb on - strand, within cysQ at 521.603 kb on - strand, within cysQ at 521.603 kb on - strand, within cysQ at 521.688 kb on + strand, within cysQ at 521.688 kb on + strand, within cysQ at 521.692 kb on + strand, within cysQ at 521.692 kb on + strand, within cysQ at 521.693 kb on - strand, within cysQ at 521.693 kb on - strand, within cysQ at 521.789 kb on + strand at 521.799 kb on + strand at 521.799 kb on + strand at 521.800 kb on - strand at 521.800 kb on - strand at 521.833 kb on + strand at 521.834 kb on - strand at 521.834 kb on - strand at 521.834 kb on - strand at 521.834 kb on - strand at 521.889 kb on + strand at 521.890 kb on - strand at 521.894 kb on - strand at 521.927 kb on + strand at 521.975 kb on + strand at 521.977 kb on + strand at 521.977 kb on + strand at 521.977 kb on + strand at 521.978 kb on - strand at 521.978 kb on - strand at 521.978 kb on - strand at 521.978 kb on - strand at 521.981 kb on + strand at 521.982 kb on - strand at 522.009 kb on + strand at 522.021 kb on + strand at 522.022 kb on - strand at 522.043 kb on - strand at 522.043 kb on - strand at 522.111 kb on + strand at 522.112 kb on - strand at 522.135 kb on + strand at 522.135 kb on + strand at 522.160 kb on + strand at 522.160 kb on + strand at 522.160 kb on + strand at 522.161 kb on - strand at 522.194 kb on + strand at 522.194 kb on + strand at 522.194 kb on + strand at 522.194 kb on + strand at 522.194 kb on + strand at 522.194 kb on + strand at 522.194 kb on + strand at 522.195 kb on - strand at 522.195 kb on - strand at 522.195 kb on - strand at 522.198 kb on + strand at 522.292 kb on + strand, within cpdB at 522.292 kb on + strand, within cpdB at 522.292 kb on + strand, within cpdB at 522.292 kb on + strand, within cpdB at 522.293 kb on - strand, within cpdB at 522.293 kb on - strand, within cpdB at 522.337 kb on + strand, within cpdB at 522.338 kb on - strand, within cpdB at 522.339 kb on + strand, within cpdB at 522.339 kb on + strand, within cpdB at 522.347 kb on + strand, within cpdB at 522.347 kb on + strand, within cpdB at 522.352 kb on - strand, within cpdB at 522.361 kb on + strand, within cpdB at 522.362 kb on - strand, within cpdB at 522.394 kb on - strand, within cpdB at 522.410 kb on - strand, within cpdB at 522.445 kb on + strand, within cpdB at 522.449 kb on + strand, within cpdB at 522.449 kb on + strand, within cpdB at 522.449 kb on + strand, within cpdB at 522.449 kb on + strand, within cpdB at 522.449 kb on + strand, within cpdB at 522.450 kb on - strand, within cpdB at 522.450 kb on - strand, within cpdB at 522.450 kb on - strand, within cpdB at 522.450 kb on - strand, within cpdB at 522.450 kb on - strand, within cpdB at 522.492 kb on + strand, within cpdB at 522.504 kb on - strand, within cpdB at 522.504 kb on - strand, within cpdB at 522.508 kb on - strand, within cpdB at 522.562 kb on + strand, within cpdB at 522.562 kb on + strand, within cpdB at 522.565 kb on - strand, within cpdB at 522.606 kb on + strand, within cpdB at 522.607 kb on - strand, within cpdB at 522.637 kb on - strand, within cpdB at 522.639 kb on + strand, within cpdB at 522.733 kb on + strand, within cpdB at 522.733 kb on + strand, within cpdB at 522.733 kb on + strand, within cpdB at 522.733 kb on + strand, within cpdB at 522.734 kb on - strand, within cpdB at 522.734 kb on - strand, within cpdB at 522.736 kb on - strand, within cpdB at 522.736 kb on - strand, within cpdB at 522.740 kb on + strand, within cpdB at 522.741 kb on - strand, within cpdB at 522.760 kb on + strand, within cpdB at 522.760 kb on + strand, within cpdB at 522.763 kb on + strand, within cpdB at 522.763 kb on + strand, within cpdB at 522.764 kb on - strand, within cpdB at 522.796 kb on - strand, within cpdB
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 520,161 - ytfI MCAODC_02545 0.22 -0.8 520,296 - ytfI MCAODC_02545 0.36 +0.5 520,296 - ytfI MCAODC_02545 0.36 +0.4 520,359 + ytfI MCAODC_02545 0.43 +0.6 520,372 - ytfI MCAODC_02545 0.44 -0.6 520,391 - ytfI MCAODC_02545 0.46 -0.2 520,483 + ytfI MCAODC_02545 0.56 +0.4 520,542 - ytfI MCAODC_02545 0.62 +1.9 520,542 - ytfI MCAODC_02545 0.62 -0.0 520,574 - ytfI MCAODC_02545 0.66 -2.2 520,662 - ytfI MCAODC_02545 0.75 -0.3 520,907 - -0.8 520,987 + +0.4 521,084 + +0.7 521,108 + +0.4 521,115 + +0.3 521,116 - -0.9 521,159 + -1.4 521,160 - -2.0 521,477 + cysQ MCAODC_02550 0.50 -4.8 521,482 + cysQ MCAODC_02550 0.51 -2.5 521,482 + cysQ MCAODC_02550 0.51 -3.3 521,482 + cysQ MCAODC_02550 0.51 -4.0 521,482 + cysQ MCAODC_02550 0.51 -2.4 521,482 + cysQ MCAODC_02550 0.51 -0.2 521,482 + cysQ MCAODC_02550 0.51 -4.0 521,483 - cysQ MCAODC_02550 0.51 -3.0 521,483 - cysQ MCAODC_02550 0.51 -3.2 521,483 - cysQ MCAODC_02550 0.51 -1.0 521,483 - cysQ MCAODC_02550 0.51 -1.7 521,483 - cysQ MCAODC_02550 0.51 -3.5 521,483 - cysQ MCAODC_02550 0.51 -3.0 521,501 + cysQ MCAODC_02550 0.53 -1.4 521,513 - cysQ MCAODC_02550 0.55 -1.8 521,513 - cysQ MCAODC_02550 0.55 -2.7 521,571 + cysQ MCAODC_02550 0.63 -2.4 521,602 + cysQ MCAODC_02550 0.67 -3.4 521,603 - cysQ MCAODC_02550 0.67 -1.6 521,603 - cysQ MCAODC_02550 0.67 -2.2 521,603 - cysQ MCAODC_02550 0.67 -2.3 521,603 - cysQ MCAODC_02550 0.67 -4.7 521,603 - cysQ MCAODC_02550 0.67 -4.2 521,688 + cysQ MCAODC_02550 0.78 -1.7 521,688 + cysQ MCAODC_02550 0.78 -1.0 521,692 + cysQ MCAODC_02550 0.79 -0.9 521,692 + cysQ MCAODC_02550 0.79 -0.3 521,693 - cysQ MCAODC_02550 0.79 -3.0 521,693 - cysQ MCAODC_02550 0.79 -3.3 521,789 + -3.9 521,799 + -2.0 521,799 + -4.0 521,800 - -2.5 521,800 - +0.6 521,833 + -1.2 521,834 - -3.0 521,834 - -3.0 521,834 - -4.8 521,834 - -5.1 521,889 + -0.8 521,890 - +0.5 521,894 - -0.4 521,927 + -1.1 521,975 + -0.6 521,977 + +0.7 521,977 + -0.8 521,977 + +0.3 521,978 - +0.7 521,978 - -0.5 521,978 - +1.5 521,978 - +0.4 521,981 + -0.7 521,982 - -2.5 522,009 + +0.2 522,021 + +0.9 522,022 - -0.5 522,043 - +0.9 522,043 - -0.1 522,111 + +1.1 522,112 - +0.3 522,135 + -0.4 522,135 + -0.4 522,160 + +0.7 522,160 + +0.2 522,160 + +0.8 522,161 - +0.1 522,194 + -0.7 522,194 + +0.3 522,194 + -0.1 522,194 + -0.2 522,194 + +0.5 522,194 + +2.6 522,194 + -0.4 522,195 - -0.5 522,195 - -0.6 522,195 - +0.5 522,198 + +0.5 522,292 + cpdB MCAODC_02560 0.13 -0.6 522,292 + cpdB MCAODC_02560 0.13 +0.1 522,292 + cpdB MCAODC_02560 0.13 +0.0 522,292 + cpdB MCAODC_02560 0.13 +0.0 522,293 - cpdB MCAODC_02560 0.13 +0.2 522,293 - cpdB MCAODC_02560 0.13 +2.6 522,337 + cpdB MCAODC_02560 0.15 +0.8 522,338 - cpdB MCAODC_02560 0.15 +0.2 522,339 + cpdB MCAODC_02560 0.16 -0.2 522,339 + cpdB MCAODC_02560 0.16 -0.6 522,347 + cpdB MCAODC_02560 0.16 +0.9 522,347 + cpdB MCAODC_02560 0.16 +0.2 522,352 - cpdB MCAODC_02560 0.16 -0.6 522,361 + cpdB MCAODC_02560 0.17 +1.2 522,362 - cpdB MCAODC_02560 0.17 -0.0 522,394 - cpdB MCAODC_02560 0.18 -2.3 522,410 - cpdB MCAODC_02560 0.19 +0.6 522,445 + cpdB MCAODC_02560 0.21 +1.7 522,449 + cpdB MCAODC_02560 0.21 +0.7 522,449 + cpdB MCAODC_02560 0.21 -0.1 522,449 + cpdB MCAODC_02560 0.21 +0.6 522,449 + cpdB MCAODC_02560 0.21 -1.2 522,449 + cpdB MCAODC_02560 0.21 -0.2 522,450 - cpdB MCAODC_02560 0.21 +1.0 522,450 - cpdB MCAODC_02560 0.21 -0.3 522,450 - cpdB MCAODC_02560 0.21 -0.2 522,450 - cpdB MCAODC_02560 0.21 -1.2 522,450 - cpdB MCAODC_02560 0.21 -0.7 522,492 + cpdB MCAODC_02560 0.23 -0.2 522,504 - cpdB MCAODC_02560 0.24 -0.6 522,504 - cpdB MCAODC_02560 0.24 -0.1 522,508 - cpdB MCAODC_02560 0.24 -1.2 522,562 + cpdB MCAODC_02560 0.27 +0.7 522,562 + cpdB MCAODC_02560 0.27 +0.9 522,565 - cpdB MCAODC_02560 0.27 -0.5 522,606 + cpdB MCAODC_02560 0.29 +0.1 522,607 - cpdB MCAODC_02560 0.29 -0.0 522,637 - cpdB MCAODC_02560 0.31 +0.7 522,639 + cpdB MCAODC_02560 0.31 -0.4 522,733 + cpdB MCAODC_02560 0.36 +1.4 522,733 + cpdB MCAODC_02560 0.36 +0.8 522,733 + cpdB MCAODC_02560 0.36 +0.5 522,733 + cpdB MCAODC_02560 0.36 -0.3 522,734 - cpdB MCAODC_02560 0.36 +1.2 522,734 - cpdB MCAODC_02560 0.36 +0.4 522,736 - cpdB MCAODC_02560 0.36 +1.0 522,736 - cpdB MCAODC_02560 0.36 +0.4 522,740 + cpdB MCAODC_02560 0.36 -0.5 522,741 - cpdB MCAODC_02560 0.36 +1.4 522,760 + cpdB MCAODC_02560 0.37 -0.6 522,760 + cpdB MCAODC_02560 0.37 -2.4 522,763 + cpdB MCAODC_02560 0.37 -1.0 522,763 + cpdB MCAODC_02560 0.37 -0.3 522,764 - cpdB MCAODC_02560 0.37 -0.9 522,796 - cpdB MCAODC_02560 0.39 +0.6
Or see this region's nucleotide sequence