Strain Fitness in Escherichia coli ECRC101 around MCAODC_01415

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfixA and caiT are separated by 473 nucleotidescaiT and caiA are separated by 30 nucleotides MCAODC_01410: fixA - Protein FixA, at 269,609 to 270,379 fixA MCAODC_01415: caiT - L-carnitine/gamma-butyrobetaine antiporter, at 270,853 to 272,367 caiT MCAODC_01420: caiA - crotonobetainyl-CoA dehydrogenase, at 272,398 to 273,540 caiA Position (kb) 270 271 272 273Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 269.870 kb on + strand, within fixAat 269.915 kb on - strand, within fixAat 269.932 kb on + strand, within fixAat 270.161 kb on - strand, within fixAat 270.188 kb on - strand, within fixAat 270.237 kb on - strand, within fixAat 270.237 kb on - strand, within fixAat 270.271 kb on - strand, within fixAat 270.278 kb on + strand, within fixAat 270.279 kb on - strand, within fixAat 270.279 kb on - strand, within fixAat 270.360 kb on + strandat 270.980 kb on + strandat 271.024 kb on + strand, within caiTat 271.025 kb on - strand, within caiTat 271.026 kb on + strand, within caiTat 271.026 kb on + strand, within caiTat 271.026 kb on + strand, within caiTat 271.027 kb on - strand, within caiTat 271.027 kb on - strand, within caiTat 271.131 kb on + strand, within caiTat 271.149 kb on + strand, within caiTat 271.189 kb on + strand, within caiTat 271.189 kb on + strand, within caiTat 271.192 kb on + strand, within caiTat 271.192 kb on + strand, within caiTat 271.193 kb on - strand, within caiTat 271.195 kb on + strand, within caiTat 271.195 kb on + strand, within caiTat 271.195 kb on + strand, within caiTat 271.196 kb on - strand, within caiTat 271.196 kb on - strand, within caiTat 271.196 kb on - strand, within caiTat 271.300 kb on + strand, within caiTat 271.300 kb on + strand, within caiTat 271.301 kb on - strand, within caiTat 271.301 kb on - strand, within caiTat 271.301 kb on - strand, within caiTat 271.327 kb on + strand, within caiTat 271.328 kb on - strand, within caiTat 271.328 kb on - strand, within caiTat 271.439 kb on - strand, within caiTat 271.439 kb on - strand, within caiTat 271.506 kb on + strand, within caiTat 271.506 kb on + strand, within caiTat 271.507 kb on - strand, within caiTat 271.507 kb on - strand, within caiTat 271.507 kb on - strand, within caiTat 271.507 kb on - strand, within caiTat 271.507 kb on - strand, within caiTat 271.533 kb on + strand, within caiTat 271.551 kb on + strand, within caiTat 271.552 kb on - strand, within caiTat 271.552 kb on - strand, within caiTat 271.560 kb on + strand, within caiTat 271.561 kb on - strand, within caiTat 271.572 kb on + strand, within caiTat 271.620 kb on + strand, within caiTat 271.621 kb on - strand, within caiTat 271.638 kb on + strand, within caiTat 271.639 kb on - strand, within caiTat 271.639 kb on - strand, within caiTat 271.642 kb on + strand, within caiTat 271.643 kb on - strand, within caiTat 271.643 kb on - strand, within caiTat 271.706 kb on - strand, within caiTat 271.706 kb on - strand, within caiTat 271.738 kb on + strand, within caiTat 271.738 kb on + strand, within caiTat 271.739 kb on - strand, within caiTat 271.759 kb on + strand, within caiTat 271.759 kb on + strand, within caiTat 271.760 kb on - strand, within caiTat 271.762 kb on - strand, within caiTat 271.810 kb on + strand, within caiTat 271.811 kb on - strand, within caiTat 271.831 kb on + strand, within caiTat 271.832 kb on - strand, within caiTat 271.878 kb on + strand, within caiTat 271.878 kb on + strand, within caiTat 271.946 kb on + strand, within caiTat 271.946 kb on + strand, within caiTat 271.947 kb on - strand, within caiTat 271.956 kb on + strand, within caiTat 271.957 kb on - strand, within caiTat 271.974 kb on - strand, within caiTat 271.977 kb on + strand, within caiTat 272.014 kb on + strand, within caiTat 272.134 kb on + strand, within caiTat 272.134 kb on + strand, within caiTat 272.134 kb on + strand, within caiTat 272.135 kb on - strand, within caiTat 272.135 kb on - strand, within caiTat 272.135 kb on - strand, within caiTat 272.135 kb on - strand, within caiTat 272.136 kb on + strand, within caiTat 272.136 kb on + strand, within caiTat 272.137 kb on - strand, within caiTat 272.137 kb on - strand, within caiTat 272.232 kb on + strandat 272.317 kb on - strandat 272.317 kb on - strandat 272.334 kb on + strandat 272.411 kb on + strandat 272.479 kb on + strandat 272.509 kb on + strandat 272.601 kb on + strand, within caiAat 272.601 kb on + strand, within caiAat 272.601 kb on + strand, within caiAat 272.602 kb on - strand, within caiAat 272.650 kb on + strand, within caiAat 272.650 kb on + strand, within caiAat 272.650 kb on + strand, within caiAat 272.650 kb on + strand, within caiAat 272.650 kb on + strand, within caiAat 272.650 kb on + strand, within caiAat 272.650 kb on + strand, within caiAat 272.650 kb on + strand, within caiAat 272.650 kb on + strand, within caiAat 272.650 kb on + strand, within caiAat 272.651 kb on - strand, within caiAat 272.651 kb on - strand, within caiAat 272.651 kb on - strand, within caiAat 272.651 kb on - strand, within caiAat 272.651 kb on - strand, within caiAat 272.651 kb on - strand, within caiAat 272.651 kb on - strand, within caiAat 272.651 kb on - strand, within caiAat 272.651 kb on - strand, within caiAat 272.651 kb on - strand, within caiAat 272.651 kb on - strand, within caiAat 272.651 kb on - strand, within caiAat 272.654 kb on + strand, within caiAat 272.654 kb on + strand, within caiAat 272.654 kb on + strand, within caiAat 272.654 kb on + strand, within caiAat 272.654 kb on + strand, within caiAat 272.654 kb on + strand, within caiAat 272.654 kb on + strand, within caiAat 272.654 kb on + strand, within caiAat 272.654 kb on + strand, within caiAat 272.654 kb on + strand, within caiAat 272.654 kb on + strand, within caiAat 272.655 kb on - strand, within caiAat 272.655 kb on - strand, within caiAat 272.655 kb on - strand, within caiAat 272.655 kb on - strand, within caiAat 272.655 kb on - strand, within caiAat 272.655 kb on - strand, within caiAat 272.655 kb on - strand, within caiAat 272.659 kb on + strand, within caiAat 272.682 kb on + strand, within caiAat 272.682 kb on + strand, within caiAat 272.682 kb on + strand, within caiAat 272.725 kb on - strand, within caiAat 272.815 kb on + strand, within caiAat 272.815 kb on + strand, within caiAat 272.815 kb on + strand, within caiAat 272.815 kb on + strand, within caiAat 272.815 kb on + strand, within caiAat 272.815 kb on + strand, within caiAat 272.815 kb on + strand, within caiAat 272.816 kb on - strand, within caiAat 272.816 kb on - strand, within caiAat 272.816 kb on - strand, within caiAat 272.816 kb on - strand, within caiAat 272.818 kb on - strand, within caiAat 272.839 kb on + strand, within caiAat 272.839 kb on + strand, within caiAat 272.839 kb on + strand, within caiAat 272.840 kb on - strand, within caiAat 272.844 kb on + strand, within caiAat 272.845 kb on - strand, within caiAat 272.845 kb on - strand, within caiAat 272.853 kb on + strand, within caiAat 272.853 kb on + strand, within caiAat 272.853 kb on + strand, within caiAat 272.853 kb on + strand, within caiAat 272.854 kb on - strand, within caiAat 272.854 kb on - strand, within caiAat 272.854 kb on - strand, within caiAat 272.863 kb on - strand, within caiAat 272.863 kb on - strand, within caiAat 272.863 kb on - strand, within caiAat 272.866 kb on - strand, within caiAat 272.866 kb on - strand, within caiAat 272.872 kb on - strand, within caiAat 272.887 kb on + strand, within caiAat 272.887 kb on + strand, within caiAat 272.888 kb on - strand, within caiAat 272.888 kb on - strand, within caiAat 272.888 kb on - strand, within caiAat 272.888 kb on - strand, within caiAat 272.955 kb on + strand, within caiAat 272.955 kb on + strand, within caiAat 272.956 kb on - strand, within caiAat 272.956 kb on - strand, within caiAat 273.013 kb on - strand, within caiAat 273.142 kb on + strand, within caiAat 273.142 kb on + strand, within caiAat 273.142 kb on + strand, within caiAat 273.142 kb on + strand, within caiAat 273.142 kb on + strand, within caiAat 273.142 kb on + strand, within caiAat 273.142 kb on + strand, within caiAat 273.143 kb on - strand, within caiAat 273.143 kb on - strand, within caiAat 273.213 kb on + strand, within caiAat 273.266 kb on + strand, within caiAat 273.289 kb on + strand, within caiAat 273.289 kb on + strand, within caiAat 273.289 kb on + strand, within caiAat 273.289 kb on + strand, within caiAat 273.289 kb on + strand, within caiAat 273.289 kb on + strand, within caiAat 273.290 kb on - strand, within caiAat 273.353 kb on - strand, within caiA

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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269,870 + fixA MCAODC_01410 0.34 +0.4
269,915 - fixA MCAODC_01410 0.40 -1.3
269,932 + fixA MCAODC_01410 0.42 -0.2
270,161 - fixA MCAODC_01410 0.72 -1.4
270,188 - fixA MCAODC_01410 0.75 -1.0
270,237 - fixA MCAODC_01410 0.81 -0.4
270,237 - fixA MCAODC_01410 0.81 -1.2
270,271 - fixA MCAODC_01410 0.86 +0.6
270,278 + fixA MCAODC_01410 0.87 -0.8
270,279 - fixA MCAODC_01410 0.87 +0.4
270,279 - fixA MCAODC_01410 0.87 +0.9
270,360 + -0.2
270,980 + +0.5
271,024 + caiT MCAODC_01415 0.11 -0.2
271,025 - caiT MCAODC_01415 0.11 -0.2
271,026 + caiT MCAODC_01415 0.11 +2.7
271,026 + caiT MCAODC_01415 0.11 +0.9
271,026 + caiT MCAODC_01415 0.11 +1.8
271,027 - caiT MCAODC_01415 0.11 -0.3
271,027 - caiT MCAODC_01415 0.11 -0.2
271,131 + caiT MCAODC_01415 0.18 +0.6
271,149 + caiT MCAODC_01415 0.20 -2.4
271,189 + caiT MCAODC_01415 0.22 +1.5
271,189 + caiT MCAODC_01415 0.22 +0.9
271,192 + caiT MCAODC_01415 0.22 +2.2
271,192 + caiT MCAODC_01415 0.22 +0.1
271,193 - caiT MCAODC_01415 0.22 +0.3
271,195 + caiT MCAODC_01415 0.23 -0.8
271,195 + caiT MCAODC_01415 0.23 +1.2
271,195 + caiT MCAODC_01415 0.23 -1.2
271,196 - caiT MCAODC_01415 0.23 -0.8
271,196 - caiT MCAODC_01415 0.23 +0.2
271,196 - caiT MCAODC_01415 0.23 -0.7
271,300 + caiT MCAODC_01415 0.30 +0.2
271,300 + caiT MCAODC_01415 0.30 -0.6
271,301 - caiT MCAODC_01415 0.30 -0.9
271,301 - caiT MCAODC_01415 0.30 +0.0
271,301 - caiT MCAODC_01415 0.30 -0.4
271,327 + caiT MCAODC_01415 0.31 +1.2
271,328 - caiT MCAODC_01415 0.31 +0.5
271,328 - caiT MCAODC_01415 0.31 -0.6
271,439 - caiT MCAODC_01415 0.39 -1.4
271,439 - caiT MCAODC_01415 0.39 -2.8
271,506 + caiT MCAODC_01415 0.43 -0.2
271,506 + caiT MCAODC_01415 0.43 -1.4
271,507 - caiT MCAODC_01415 0.43 +0.3
271,507 - caiT MCAODC_01415 0.43 -1.9
271,507 - caiT MCAODC_01415 0.43 -0.1
271,507 - caiT MCAODC_01415 0.43 +0.9
271,507 - caiT MCAODC_01415 0.43 +0.7
271,533 + caiT MCAODC_01415 0.45 +0.1
271,551 + caiT MCAODC_01415 0.46 +0.3
271,552 - caiT MCAODC_01415 0.46 -1.6
271,552 - caiT MCAODC_01415 0.46 +0.8
271,560 + caiT MCAODC_01415 0.47 +1.5
271,561 - caiT MCAODC_01415 0.47 -1.4
271,572 + caiT MCAODC_01415 0.47 +0.3
271,620 + caiT MCAODC_01415 0.51 -2.0
271,621 - caiT MCAODC_01415 0.51 +0.2
271,638 + caiT MCAODC_01415 0.52 +0.0
271,639 - caiT MCAODC_01415 0.52 +0.6
271,639 - caiT MCAODC_01415 0.52 +0.6
271,642 + caiT MCAODC_01415 0.52 -1.3
271,643 - caiT MCAODC_01415 0.52 +0.7
271,643 - caiT MCAODC_01415 0.52 -1.0
271,706 - caiT MCAODC_01415 0.56 +1.6
271,706 - caiT MCAODC_01415 0.56 +0.1
271,738 + caiT MCAODC_01415 0.58 -1.8
271,738 + caiT MCAODC_01415 0.58 -0.3
271,739 - caiT MCAODC_01415 0.58 -2.4
271,759 + caiT MCAODC_01415 0.60 -0.4
271,759 + caiT MCAODC_01415 0.60 -0.5
271,760 - caiT MCAODC_01415 0.60 -0.1
271,762 - caiT MCAODC_01415 0.60 +0.4
271,810 + caiT MCAODC_01415 0.63 +1.7
271,811 - caiT MCAODC_01415 0.63 +0.8
271,831 + caiT MCAODC_01415 0.65 -1.5
271,832 - caiT MCAODC_01415 0.65 -0.3
271,878 + caiT MCAODC_01415 0.68 -3.5
271,878 + caiT MCAODC_01415 0.68 +0.0
271,946 + caiT MCAODC_01415 0.72 +2.3
271,946 + caiT MCAODC_01415 0.72 +1.9
271,947 - caiT MCAODC_01415 0.72 -1.9
271,956 + caiT MCAODC_01415 0.73 -0.7
271,957 - caiT MCAODC_01415 0.73 +1.3
271,974 - caiT MCAODC_01415 0.74 +0.5
271,977 + caiT MCAODC_01415 0.74 +0.5
272,014 + caiT MCAODC_01415 0.77 +0.4
272,134 + caiT MCAODC_01415 0.85 +0.3
272,134 + caiT MCAODC_01415 0.85 -0.0
272,134 + caiT MCAODC_01415 0.85 -1.4
272,135 - caiT MCAODC_01415 0.85 -1.7
272,135 - caiT MCAODC_01415 0.85 -0.8
272,135 - caiT MCAODC_01415 0.85 -0.7
272,135 - caiT MCAODC_01415 0.85 -0.5
272,136 + caiT MCAODC_01415 0.85 +0.1
272,136 + caiT MCAODC_01415 0.85 +0.1
272,137 - caiT MCAODC_01415 0.85 +0.4
272,137 - caiT MCAODC_01415 0.85 -1.2
272,232 + -2.9
272,317 - -2.5
272,317 - +0.2
272,334 + -0.0
272,411 + -0.4
272,479 + +0.0
272,509 + +0.3
272,601 + caiA MCAODC_01420 0.18 +0.5
272,601 + caiA MCAODC_01420 0.18 -1.6
272,601 + caiA MCAODC_01420 0.18 -0.6
272,602 - caiA MCAODC_01420 0.18 +1.4
272,650 + caiA MCAODC_01420 0.22 -0.5
272,650 + caiA MCAODC_01420 0.22 +0.1
272,650 + caiA MCAODC_01420 0.22 -0.4
272,650 + caiA MCAODC_01420 0.22 +1.0
272,650 + caiA MCAODC_01420 0.22 +0.1
272,650 + caiA MCAODC_01420 0.22 +1.3
272,650 + caiA MCAODC_01420 0.22 +0.2
272,650 + caiA MCAODC_01420 0.22 -1.5
272,650 + caiA MCAODC_01420 0.22 +0.6
272,650 + caiA MCAODC_01420 0.22 -0.5
272,651 - caiA MCAODC_01420 0.22 +0.9
272,651 - caiA MCAODC_01420 0.22 +1.4
272,651 - caiA MCAODC_01420 0.22 +0.3
272,651 - caiA MCAODC_01420 0.22 +0.4
272,651 - caiA MCAODC_01420 0.22 -0.9
272,651 - caiA MCAODC_01420 0.22 -0.3
272,651 - caiA MCAODC_01420 0.22 +2.0
272,651 - caiA MCAODC_01420 0.22 +1.3
272,651 - caiA MCAODC_01420 0.22 +0.4
272,651 - caiA MCAODC_01420 0.22 -0.1
272,651 - caiA MCAODC_01420 0.22 +0.6
272,651 - caiA MCAODC_01420 0.22 +1.6
272,654 + caiA MCAODC_01420 0.22 -0.6
272,654 + caiA MCAODC_01420 0.22 +0.5
272,654 + caiA MCAODC_01420 0.22 +1.1
272,654 + caiA MCAODC_01420 0.22 +0.1
272,654 + caiA MCAODC_01420 0.22 +0.2
272,654 + caiA MCAODC_01420 0.22 +0.3
272,654 + caiA MCAODC_01420 0.22 -0.1
272,654 + caiA MCAODC_01420 0.22 -0.7
272,654 + caiA MCAODC_01420 0.22 +1.5
272,654 + caiA MCAODC_01420 0.22 +2.5
272,654 + caiA MCAODC_01420 0.22 -0.5
272,655 - caiA MCAODC_01420 0.22 +0.2
272,655 - caiA MCAODC_01420 0.22 +0.3
272,655 - caiA MCAODC_01420 0.22 +0.6
272,655 - caiA MCAODC_01420 0.22 +1.3
272,655 - caiA MCAODC_01420 0.22 -1.1
272,655 - caiA MCAODC_01420 0.22 -1.8
272,655 - caiA MCAODC_01420 0.22 +1.3
272,659 + caiA MCAODC_01420 0.23 +0.3
272,682 + caiA MCAODC_01420 0.25 +1.6
272,682 + caiA MCAODC_01420 0.25 -1.7
272,682 + caiA MCAODC_01420 0.25 -1.1
272,725 - caiA MCAODC_01420 0.29 -1.0
272,815 + caiA MCAODC_01420 0.36 +2.1
272,815 + caiA MCAODC_01420 0.36 +1.5
272,815 + caiA MCAODC_01420 0.36 -0.3
272,815 + caiA MCAODC_01420 0.36 -2.2
272,815 + caiA MCAODC_01420 0.36 +0.1
272,815 + caiA MCAODC_01420 0.36 -0.8
272,815 + caiA MCAODC_01420 0.36 +1.7
272,816 - caiA MCAODC_01420 0.37 -0.1
272,816 - caiA MCAODC_01420 0.37 -0.3
272,816 - caiA MCAODC_01420 0.37 +0.7
272,816 - caiA MCAODC_01420 0.37 -2.8
272,818 - caiA MCAODC_01420 0.37 -1.4
272,839 + caiA MCAODC_01420 0.39 +1.4
272,839 + caiA MCAODC_01420 0.39 -1.1
272,839 + caiA MCAODC_01420 0.39 +0.2
272,840 - caiA MCAODC_01420 0.39 +0.4
272,844 + caiA MCAODC_01420 0.39 -0.3
272,845 - caiA MCAODC_01420 0.39 +1.0
272,845 - caiA MCAODC_01420 0.39 -1.5
272,853 + caiA MCAODC_01420 0.40 +0.4
272,853 + caiA MCAODC_01420 0.40 +2.2
272,853 + caiA MCAODC_01420 0.40 -0.3
272,853 + caiA MCAODC_01420 0.40 -0.3
272,854 - caiA MCAODC_01420 0.40 +0.0
272,854 - caiA MCAODC_01420 0.40 +0.1
272,854 - caiA MCAODC_01420 0.40 +0.1
272,863 - caiA MCAODC_01420 0.41 +0.4
272,863 - caiA MCAODC_01420 0.41 +0.3
272,863 - caiA MCAODC_01420 0.41 +0.4
272,866 - caiA MCAODC_01420 0.41 +0.9
272,866 - caiA MCAODC_01420 0.41 +1.8
272,872 - caiA MCAODC_01420 0.41 +0.4
272,887 + caiA MCAODC_01420 0.43 -0.9
272,887 + caiA MCAODC_01420 0.43 -0.6
272,888 - caiA MCAODC_01420 0.43 +0.3
272,888 - caiA MCAODC_01420 0.43 -0.2
272,888 - caiA MCAODC_01420 0.43 +0.9
272,888 - caiA MCAODC_01420 0.43 -1.4
272,955 + caiA MCAODC_01420 0.49 -0.4
272,955 + caiA MCAODC_01420 0.49 -0.5
272,956 - caiA MCAODC_01420 0.49 +0.9
272,956 - caiA MCAODC_01420 0.49 -0.5
273,013 - caiA MCAODC_01420 0.54 +0.3
273,142 + caiA MCAODC_01420 0.65 -0.1
273,142 + caiA MCAODC_01420 0.65 +0.0
273,142 + caiA MCAODC_01420 0.65 +1.0
273,142 + caiA MCAODC_01420 0.65 -1.2
273,142 + caiA MCAODC_01420 0.65 +0.1
273,142 + caiA MCAODC_01420 0.65 -0.5
273,142 + caiA MCAODC_01420 0.65 -2.5
273,143 - caiA MCAODC_01420 0.65 -0.6
273,143 - caiA MCAODC_01420 0.65 -0.1
273,213 + caiA MCAODC_01420 0.71 -0.3
273,266 + caiA MCAODC_01420 0.76 +1.7
273,289 + caiA MCAODC_01420 0.78 +0.2
273,289 + caiA MCAODC_01420 0.78 +0.4
273,289 + caiA MCAODC_01420 0.78 -2.9
273,289 + caiA MCAODC_01420 0.78 -1.3
273,289 + caiA MCAODC_01420 0.78 -0.8
273,289 + caiA MCAODC_01420 0.78 -0.7
273,290 - caiA MCAODC_01420 0.78 -0.4
273,353 - caiA MCAODC_01420 0.84 +0.0

Or see this region's nucleotide sequence