Experiment: Cytosine 0.9 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS10700 and A4249_RS10705 overlap by 4 nucleotides A4249_RS10705 and A4249_RS10710 overlap by 7 nucleotides A4249_RS10710 and A4249_RS10715 are separated by 31 nucleotides
A4249_RS10700: A4249_RS10700 - imidazoleglycerol-phosphate dehydratase HisB, at 2,151,084 to 2,152,520
_RS10700
A4249_RS10705: A4249_RS10705 - imidazole glycerol phosphate synthase subunit HisH, at 2,152,517 to 2,153,119
_RS10705
A4249_RS10710: A4249_RS10710 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase, at 2,153,113 to 2,153,850
_RS10710
A4249_RS10715: A4249_RS10715 - imidazole glycerol phosphate synthase subunit HisF, at 2,153,882 to 2,154,649
_RS10715
Position (kb)
2152
2153
2154 Strain fitness (log2 ratio)
-1
0
1 at 2151.538 kb on - strand, within A4249_RS10700 at 2151.619 kb on - strand, within A4249_RS10700 at 2151.619 kb on - strand, within A4249_RS10700 at 2151.619 kb on - strand, within A4249_RS10700 at 2151.619 kb on - strand, within A4249_RS10700 at 2151.831 kb on + strand, within A4249_RS10700 at 2151.831 kb on + strand, within A4249_RS10700 at 2151.832 kb on - strand, within A4249_RS10700 at 2151.832 kb on - strand, within A4249_RS10700 at 2152.284 kb on + strand, within A4249_RS10700 at 2152.376 kb on + strand, within A4249_RS10700 at 2152.440 kb on + strand at 2152.441 kb on - strand at 2152.441 kb on - strand at 2152.441 kb on - strand at 2152.441 kb on - strand at 2152.659 kb on + strand, within A4249_RS10705 at 2152.659 kb on + strand, within A4249_RS10705 at 2152.660 kb on - strand, within A4249_RS10705 at 2152.813 kb on + strand, within A4249_RS10705 at 2152.935 kb on - strand, within A4249_RS10705 at 2152.935 kb on - strand, within A4249_RS10705 at 2152.935 kb on - strand, within A4249_RS10705 at 2152.935 kb on - strand, within A4249_RS10705 at 2152.935 kb on - strand, within A4249_RS10705 at 2152.935 kb on - strand, within A4249_RS10705 at 2152.940 kb on + strand, within A4249_RS10705 at 2152.941 kb on - strand, within A4249_RS10705 at 2152.941 kb on - strand, within A4249_RS10705 at 2152.997 kb on + strand, within A4249_RS10705 at 2152.998 kb on - strand, within A4249_RS10705 at 2153.122 kb on + strand at 2153.123 kb on - strand at 2153.194 kb on + strand, within A4249_RS10710 at 2153.195 kb on - strand, within A4249_RS10710 at 2153.797 kb on + strand at 2153.916 kb on + strand at 2153.916 kb on + strand at 2153.917 kb on - strand at 2153.917 kb on - strand at 2154.029 kb on + strand, within A4249_RS10715 at 2154.029 kb on + strand, within A4249_RS10715 at 2154.029 kb on + strand, within A4249_RS10715 at 2154.029 kb on + strand, within A4249_RS10715 at 2154.029 kb on + strand, within A4249_RS10715 at 2154.030 kb on - strand, within A4249_RS10715 at 2154.030 kb on - strand, within A4249_RS10715 at 2154.030 kb on - strand, within A4249_RS10715 at 2154.030 kb on - strand, within A4249_RS10715 at 2154.030 kb on - strand, within A4249_RS10715 at 2154.030 kb on - strand, within A4249_RS10715 at 2154.068 kb on + strand, within A4249_RS10715 at 2154.068 kb on + strand, within A4249_RS10715 at 2154.069 kb on - strand, within A4249_RS10715
Per-strain Table
Position Strand Gene LocusTag Fraction Cytosine 0.9 mM (C) remove 2,151,538 - A4249_RS10700 0.32 -0.5 2,151,619 - A4249_RS10700 0.37 +0.1 2,151,619 - A4249_RS10700 0.37 +0.1 2,151,619 - A4249_RS10700 0.37 -0.4 2,151,619 - A4249_RS10700 0.37 -1.2 2,151,831 + A4249_RS10700 0.52 +0.1 2,151,831 + A4249_RS10700 0.52 +0.1 2,151,832 - A4249_RS10700 0.52 +0.2 2,151,832 - A4249_RS10700 0.52 +0.2 2,152,284 + A4249_RS10700 0.84 +1.0 2,152,376 + A4249_RS10700 0.90 +1.6 2,152,440 + +0.9 2,152,441 - +0.5 2,152,441 - +0.2 2,152,441 - +0.4 2,152,441 - -0.9 2,152,659 + A4249_RS10705 0.24 -0.1 2,152,659 + A4249_RS10705 0.24 -0.1 2,152,660 - A4249_RS10705 0.24 -0.6 2,152,813 + A4249_RS10705 0.49 -0.2 2,152,935 - A4249_RS10705 0.69 +0.0 2,152,935 - A4249_RS10705 0.69 +0.2 2,152,935 - A4249_RS10705 0.69 -0.2 2,152,935 - A4249_RS10705 0.69 -1.1 2,152,935 - A4249_RS10705 0.69 +0.7 2,152,935 - A4249_RS10705 0.69 -0.1 2,152,940 + A4249_RS10705 0.70 +0.8 2,152,941 - A4249_RS10705 0.70 -0.6 2,152,941 - A4249_RS10705 0.70 -0.3 2,152,997 + A4249_RS10705 0.80 +0.1 2,152,998 - A4249_RS10705 0.80 -0.5 2,153,122 + +0.1 2,153,123 - -0.1 2,153,194 + A4249_RS10710 0.11 -0.6 2,153,195 - A4249_RS10710 0.11 -0.2 2,153,797 + +1.0 2,153,916 + -0.2 2,153,916 + +0.4 2,153,917 - +1.6 2,153,917 - +0.5 2,154,029 + A4249_RS10715 0.19 +0.1 2,154,029 + A4249_RS10715 0.19 +0.5 2,154,029 + A4249_RS10715 0.19 -1.1 2,154,029 + A4249_RS10715 0.19 -1.2 2,154,029 + A4249_RS10715 0.19 -0.4 2,154,030 - A4249_RS10715 0.19 -0.2 2,154,030 - A4249_RS10715 0.19 -0.0 2,154,030 - A4249_RS10715 0.19 +0.7 2,154,030 - A4249_RS10715 0.19 +0.4 2,154,030 - A4249_RS10715 0.19 +0.2 2,154,030 - A4249_RS10715 0.19 -0.7 2,154,068 + A4249_RS10715 0.24 -0.5 2,154,068 + A4249_RS10715 0.24 -0.6 2,154,069 - A4249_RS10715 0.24 -0.9
Or see this region's nucleotide sequence