Experiment: Cytosine 0.9 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS01020 and A4249_RS01025 are separated by 60 nucleotides A4249_RS01025 and A4249_RS01030 are separated by 143 nucleotides A4249_RS01030 and A4249_RS01035 are separated by 261 nucleotides
A4249_RS01020: A4249_RS01020 - SDR family NAD(P)-dependent oxidoreductase, at 193,546 to 194,223
_RS01020
A4249_RS01025: A4249_RS01025 - GAF domain-containing protein, at 194,284 to 194,781
_RS01025
A4249_RS01030: A4249_RS01030 - hypothetical protein, at 194,925 to 195,422
_RS01030
A4249_RS01035: A4249_RS01035 - S8 family serine peptidase, at 195,684 to 198,932
_RS01035
Position (kb) 194
195
196 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 194.152 kb on + strand, within A4249_RS01020 at 194.152 kb on + strand, within A4249_RS01020 at 194.153 kb on - strand, within A4249_RS01020 at 194.153 kb on - strand, within A4249_RS01020 at 194.199 kb on + strand at 194.200 kb on - strand at 194.250 kb on + strand at 194.250 kb on + strand at 194.250 kb on + strand at 194.250 kb on + strand at 194.250 kb on + strand at 194.250 kb on + strand at 194.250 kb on + strand at 194.250 kb on + strand at 194.250 kb on + strand at 194.250 kb on + strand at 194.250 kb on + strand at 194.251 kb on - strand at 194.251 kb on - strand at 194.321 kb on - strand at 194.321 kb on - strand at 194.564 kb on + strand, within A4249_RS01025 at 194.564 kb on + strand, within A4249_RS01025 at 194.564 kb on + strand, within A4249_RS01025 at 194.609 kb on + strand, within A4249_RS01025 at 194.609 kb on + strand, within A4249_RS01025 at 194.609 kb on + strand, within A4249_RS01025 at 194.609 kb on + strand, within A4249_RS01025 at 194.609 kb on + strand, within A4249_RS01025 at 194.609 kb on + strand, within A4249_RS01025 at 194.609 kb on + strand, within A4249_RS01025 at 194.609 kb on + strand, within A4249_RS01025 at 194.609 kb on + strand, within A4249_RS01025 at 194.609 kb on + strand, within A4249_RS01025 at 194.609 kb on + strand, within A4249_RS01025 at 194.609 kb on + strand, within A4249_RS01025 at 194.609 kb on + strand, within A4249_RS01025 at 194.609 kb on + strand, within A4249_RS01025 at 194.609 kb on + strand, within A4249_RS01025 at 194.610 kb on - strand, within A4249_RS01025 at 194.610 kb on - strand, within A4249_RS01025 at 194.610 kb on - strand, within A4249_RS01025 at 194.610 kb on - strand, within A4249_RS01025 at 194.610 kb on - strand, within A4249_RS01025 at 194.610 kb on - strand, within A4249_RS01025 at 194.610 kb on - strand, within A4249_RS01025 at 194.610 kb on - strand, within A4249_RS01025 at 194.610 kb on - strand, within A4249_RS01025 at 194.610 kb on - strand, within A4249_RS01025 at 194.610 kb on - strand, within A4249_RS01025 at 194.627 kb on + strand, within A4249_RS01025 at 194.628 kb on - strand, within A4249_RS01025 at 194.628 kb on - strand, within A4249_RS01025 at 194.680 kb on + strand, within A4249_RS01025 at 194.680 kb on + strand, within A4249_RS01025 at 194.681 kb on - strand, within A4249_RS01025 at 194.732 kb on + strand at 194.774 kb on + strand at 194.774 kb on + strand at 194.775 kb on - strand at 194.775 kb on - strand at 194.775 kb on - strand at 194.775 kb on - strand at 194.775 kb on - strand at 194.775 kb on - strand at 194.775 kb on - strand at 194.813 kb on + strand at 194.814 kb on - strand at 194.814 kb on - strand at 194.815 kb on + strand at 194.815 kb on + strand at 194.815 kb on + strand at 194.816 kb on - strand at 194.816 kb on - strand at 194.816 kb on - strand at 195.159 kb on + strand, within A4249_RS01030 at 195.159 kb on + strand, within A4249_RS01030 at 195.159 kb on + strand, within A4249_RS01030 at 195.159 kb on + strand, within A4249_RS01030 at 195.159 kb on + strand, within A4249_RS01030 at 195.159 kb on + strand, within A4249_RS01030 at 195.159 kb on + strand, within A4249_RS01030 at 195.160 kb on - strand, within A4249_RS01030 at 195.160 kb on - strand, within A4249_RS01030 at 195.160 kb on - strand, within A4249_RS01030 at 195.160 kb on - strand, within A4249_RS01030 at 195.160 kb on - strand, within A4249_RS01030 at 195.160 kb on - strand, within A4249_RS01030 at 195.160 kb on - strand, within A4249_RS01030 at 195.160 kb on - strand, within A4249_RS01030 at 195.160 kb on - strand, within A4249_RS01030 at 195.160 kb on - strand, within A4249_RS01030 at 195.207 kb on + strand, within A4249_RS01030 at 195.207 kb on + strand, within A4249_RS01030 at 195.207 kb on + strand, within A4249_RS01030 at 195.228 kb on + strand, within A4249_RS01030 at 195.229 kb on - strand, within A4249_RS01030 at 195.487 kb on + strand at 195.595 kb on + strand at 195.595 kb on + strand at 195.595 kb on + strand at 195.595 kb on + strand at 195.596 kb on - strand at 195.624 kb on + strand at 195.624 kb on + strand at 195.658 kb on + strand at 195.747 kb on + strand at 195.747 kb on + strand at 195.747 kb on + strand at 195.747 kb on + strand at 195.747 kb on + strand at 195.748 kb on - strand at 195.748 kb on - strand at 195.748 kb on - strand at 195.748 kb on - strand at 195.748 kb on - strand at 195.748 kb on - strand at 195.748 kb on - strand at 195.777 kb on + strand at 195.777 kb on + strand at 195.777 kb on + strand at 195.778 kb on - strand at 195.778 kb on - strand at 195.778 kb on - strand at 195.778 kb on - strand at 195.778 kb on - strand at 195.778 kb on - strand at 195.861 kb on + strand at 195.861 kb on + strand at 195.861 kb on + strand at 195.862 kb on - strand at 195.862 kb on - strand at 195.862 kb on - strand at 195.862 kb on - strand at 195.918 kb on + strand at 195.918 kb on + strand at 195.918 kb on + strand at 195.918 kb on + strand at 195.918 kb on + strand at 195.918 kb on + strand at 195.918 kb on + strand at 195.918 kb on + strand at 195.919 kb on - strand at 195.919 kb on - strand at 195.919 kb on - strand at 195.919 kb on - strand at 195.919 kb on - strand at 196.158 kb on + strand, within A4249_RS01035 at 196.158 kb on + strand, within A4249_RS01035 at 196.158 kb on + strand, within A4249_RS01035 at 196.158 kb on + strand, within A4249_RS01035 at 196.159 kb on - strand, within A4249_RS01035 at 196.159 kb on - strand, within A4249_RS01035 at 196.326 kb on + strand, within A4249_RS01035 at 196.326 kb on + strand, within A4249_RS01035 at 196.327 kb on - strand, within A4249_RS01035 at 196.327 kb on - strand, within A4249_RS01035 at 196.327 kb on - strand, within A4249_RS01035 at 196.327 kb on - strand, within A4249_RS01035 at 196.338 kb on + strand, within A4249_RS01035 at 196.338 kb on + strand, within A4249_RS01035 at 196.339 kb on - strand, within A4249_RS01035 at 196.339 kb on - strand, within A4249_RS01035
Per-strain Table
Position Strand Gene LocusTag Fraction Cytosine 0.9 mM (C) remove 194,152 + A4249_RS01020 0.89 +0.3 194,152 + A4249_RS01020 0.89 +0.2 194,153 - A4249_RS01020 0.90 -0.1 194,153 - A4249_RS01020 0.90 -1.1 194,199 + -1.2 194,200 - -0.1 194,250 + -0.8 194,250 + -0.4 194,250 + +0.1 194,250 + -0.2 194,250 + +0.3 194,250 + +0.5 194,250 + +0.0 194,250 + -0.5 194,250 + -0.3 194,250 + +0.4 194,250 + +1.0 194,251 - +0.3 194,251 - -0.8 194,321 - +0.3 194,321 - +0.0 194,564 + A4249_RS01025 0.56 -0.1 194,564 + A4249_RS01025 0.56 -0.1 194,564 + A4249_RS01025 0.56 -0.5 194,609 + A4249_RS01025 0.65 +0.4 194,609 + A4249_RS01025 0.65 +0.1 194,609 + A4249_RS01025 0.65 +0.1 194,609 + A4249_RS01025 0.65 -0.2 194,609 + A4249_RS01025 0.65 +0.1 194,609 + A4249_RS01025 0.65 +0.8 194,609 + A4249_RS01025 0.65 +0.1 194,609 + A4249_RS01025 0.65 -0.6 194,609 + A4249_RS01025 0.65 -1.0 194,609 + A4249_RS01025 0.65 +0.2 194,609 + A4249_RS01025 0.65 +0.5 194,609 + A4249_RS01025 0.65 -2.1 194,609 + A4249_RS01025 0.65 -0.2 194,609 + A4249_RS01025 0.65 +0.4 194,609 + A4249_RS01025 0.65 -0.3 194,610 - A4249_RS01025 0.65 +0.2 194,610 - A4249_RS01025 0.65 -0.5 194,610 - A4249_RS01025 0.65 +0.2 194,610 - A4249_RS01025 0.65 -0.6 194,610 - A4249_RS01025 0.65 -1.0 194,610 - A4249_RS01025 0.65 -0.1 194,610 - A4249_RS01025 0.65 +0.0 194,610 - A4249_RS01025 0.65 +0.4 194,610 - A4249_RS01025 0.65 -0.1 194,610 - A4249_RS01025 0.65 -0.0 194,610 - A4249_RS01025 0.65 -0.7 194,627 + A4249_RS01025 0.69 +0.8 194,628 - A4249_RS01025 0.69 -0.5 194,628 - A4249_RS01025 0.69 -0.4 194,680 + A4249_RS01025 0.80 -0.1 194,680 + A4249_RS01025 0.80 +0.3 194,681 - A4249_RS01025 0.80 -0.1 194,732 + -0.3 194,774 + -0.0 194,774 + -0.4 194,775 - -0.3 194,775 - +0.2 194,775 - +1.0 194,775 - +0.2 194,775 - -0.0 194,775 - +0.4 194,775 - +0.2 194,813 + -0.5 194,814 - -0.1 194,814 - +0.5 194,815 + -0.3 194,815 + +0.0 194,815 + -0.4 194,816 - -0.1 194,816 - -0.5 194,816 - +0.6 195,159 + A4249_RS01030 0.47 +0.0 195,159 + A4249_RS01030 0.47 +0.2 195,159 + A4249_RS01030 0.47 +0.7 195,159 + A4249_RS01030 0.47 -0.5 195,159 + A4249_RS01030 0.47 +0.2 195,159 + A4249_RS01030 0.47 +0.4 195,159 + A4249_RS01030 0.47 -0.4 195,160 - A4249_RS01030 0.47 +0.0 195,160 - A4249_RS01030 0.47 +0.6 195,160 - A4249_RS01030 0.47 -1.1 195,160 - A4249_RS01030 0.47 -0.3 195,160 - A4249_RS01030 0.47 -0.4 195,160 - A4249_RS01030 0.47 -0.7 195,160 - A4249_RS01030 0.47 -0.2 195,160 - A4249_RS01030 0.47 +0.3 195,160 - A4249_RS01030 0.47 +0.2 195,160 - A4249_RS01030 0.47 +1.1 195,207 + A4249_RS01030 0.57 +1.0 195,207 + A4249_RS01030 0.57 +1.3 195,207 + A4249_RS01030 0.57 -0.7 195,228 + A4249_RS01030 0.61 +0.2 195,229 - A4249_RS01030 0.61 -0.3 195,487 + +1.4 195,595 + -0.7 195,595 + -0.2 195,595 + -0.1 195,595 + -0.2 195,596 - -0.1 195,624 + -0.2 195,624 + -0.5 195,658 + +0.2 195,747 + -0.7 195,747 + -0.2 195,747 + -1.9 195,747 + -0.1 195,747 + -0.1 195,748 - +0.0 195,748 - -0.1 195,748 - +1.1 195,748 - +0.4 195,748 - +0.0 195,748 - +0.0 195,748 - +1.1 195,777 + -0.5 195,777 + +0.3 195,777 + +0.2 195,778 - +0.6 195,778 - +0.1 195,778 - +0.1 195,778 - -0.8 195,778 - -0.5 195,778 - -0.1 195,861 + +0.3 195,861 + -0.3 195,861 + +1.1 195,862 - -0.5 195,862 - -0.1 195,862 - +0.0 195,862 - -0.5 195,918 + +0.2 195,918 + -2.9 195,918 + -0.6 195,918 + +0.2 195,918 + +0.1 195,918 + +0.2 195,918 + +0.0 195,918 + +0.2 195,919 - -0.9 195,919 - +0.1 195,919 - -0.3 195,919 - -2.1 195,919 - +0.6 196,158 + A4249_RS01035 0.15 -0.1 196,158 + A4249_RS01035 0.15 +0.2 196,158 + A4249_RS01035 0.15 +0.5 196,158 + A4249_RS01035 0.15 +0.1 196,159 - A4249_RS01035 0.15 -0.0 196,159 - A4249_RS01035 0.15 -0.3 196,326 + A4249_RS01035 0.20 +0.1 196,326 + A4249_RS01035 0.20 +0.1 196,327 - A4249_RS01035 0.20 +0.5 196,327 - A4249_RS01035 0.20 +0.1 196,327 - A4249_RS01035 0.20 -0.1 196,327 - A4249_RS01035 0.20 +0.4 196,338 + A4249_RS01035 0.20 -0.3 196,338 + A4249_RS01035 0.20 -0.7 196,339 - A4249_RS01035 0.20 -0.3 196,339 - A4249_RS01035 0.20 +0.4
Or see this region's nucleotide sequence